Plant Stress最新文献

{"title":"Multi-level approach to screen tomato inbred lines for resilience to Ni-enriched soils and water deficit","authors":"Daniela Fortini ,&nbsp;Roberto Chignola ,&nbsp;Giulio Zanni ,&nbsp;Alice Brunazzi ,&nbsp;Anita Zamboni ,&nbsp;Gianni Zoccatelli ,&nbsp;Marco Ciulu ,&nbsp;Diana Vanessa Santisteban Soto ,&nbsp;Tommaso Sanson ,&nbsp;Tiziana Pandolfini ,&nbsp;Barbara Molesini","doi":"10.1016/j.stress.2025.100794","DOIUrl":"10.1016/j.stress.2025.100794","url":null,"abstract":"<div><div>Climate-driven increases in drought occurrence and the contamination of soils by heavy metals adversely affect tomato production both qualitatively and quantitatively. The aim of the present study was to investigate the responses of four inbred lines of <em>Solanum lycopersicum</em> to Ni toxicity and water deprivation. To evaluate the effects of Ni, we used two different growth approaches, in hydroponics and in soil, associating a morphological analysis of the lines with Ni and mineral nutrient quantification in both shoots and fruits. The effects of water stress were tested on germination capacity <em>in vitro</em> and on adult plants grown in soil. The responses of the different lines to water stress were assessed by physiological and phenotypic analyses, expression of drought-related genes and quantification of ABA. The multi-level approach allowed us to identify two lines, among the four investigated, as good candidates for future breeding programs due to their ability to accumulate less Ni and maintain fruit quality parameters and capacity to acclimate to repeated water stress.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100794"},"PeriodicalIF":6.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Rice sucrose non-fermenting related protein kinase (SnRK1) has a limited role in defense against Fall armyworm (Spodoptera frugiperda)” [Plant Stress, Volume 14, December 2024, 100667]","authors":"Devi Balakrishnan ,&nbsp;Vibha Srivastava ,&nbsp;Rupesh Kariyat","doi":"10.1016/j.stress.2025.100758","DOIUrl":"10.1016/j.stress.2025.100758","url":null,"abstract":"","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100758"},"PeriodicalIF":6.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143632172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated analysis of metabolomics and transcriptomics reveals the metabolites responsible for the antioxidant activity of water caltrop hull","authors":"Mingya Fang ,&nbsp;Guanhua Liu ,&nbsp;Wei Sheng ,&nbsp;Xiaoyang Chen ,&nbsp;Lin Shi ,&nbsp;Xiangtan Yao ,&nbsp;Lingyun Wang ,&nbsp;Zhaisheng Zheng","doi":"10.1016/j.stress.2025.100746","DOIUrl":"10.1016/j.stress.2025.100746","url":null,"abstract":"<div><div>The water caltrop (<em>Trapa</em> genus, Lythraceae family) fruit hulls are used as traditional herb medicines in China, Korea, and India due to their secondary metabolites. Although the hulls of multiple water caltrop varieties are applied as herb in these countries, few studies have assessed the metabolite diversity across water caltrop varieties. Here, six representative water caltrop varieties which were distributed in China and significant phenotypic difference, were chosen as the model to analysis the metabolome variation. The wild water caltrop and cultivated water caltrop have significant differences in size, volume, and weight of the fruit. The hulls of water caltrop had abundant secondary metabolites, including flavonoids, polyphenols, polysaccharides, and terpenoids. The metabolomes of the cultivated water caltrops were quite different from those of the wild ones. The abundance of flavonoids in the cultivated water caltrop hulls was lower than that in the wild hulls based on the UPL-MS analysis. The flavonoid composition also differed significantly among the water caltrop varieties. For instance, kaempferol-4′-<em>O</em>-glucoside was absent in three wild water caltrop varieties (Sijiaoyeling, Sliuling, and Geling). The antioxidant activities of the hull extracts of the cultivated water caltrop, SHL and WL, were similar. On the contrary, the extract of the wild water caltrops showed variation in both DPPH radical-scavenging and ferric reducing/antioxidant power. Subsequent analysis indicated that the flavonoids abundance was strongly correlated with the antioxidant activity. Besides, variations were detected in the expression levels of genes associated with flavonoid biosynthesis among the six water caltrop hulls. Comprehensively analysis of the metabolome and antioxidant activity of wild and cultivated species of water caltrop provide a new perspective for utilizing water caltrop varieties.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"16 ","pages":"Article 100746"},"PeriodicalIF":6.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal and plant-growth promoting potency of Streptomyces rochei against biotic stress caused by Race 4 Fusarium wilt on banana","authors":"Periakaruppan Jegan ,&nbsp;Saraswathy Sethurathinam ,&nbsp;Muthuvel Iyyamperumal ,&nbsp;Rajangam Jacob ,&nbsp;Angappan Kathithachalam ,&nbsp;Jayakanthan Mannu ,&nbsp;Soman Padmanabhan ,&nbsp;Manimaran Gajendiran","doi":"10.1016/j.stress.2025.100779","DOIUrl":"10.1016/j.stress.2025.100779","url":null,"abstract":"<div><div>Banana is a staple food for millions of individuals, especially in regions with tropical and subtropical climates. Nevertheless, the cultivation of banana is under significant threat from <em>Fusarium</em> wilt, a harmful biotic stress transmitted through the soil and caused by <em>Fusarium oxysporum</em> f. sp. <em>cubense</em> (<em>Foc</em>). This disease has the potential to devastate and infect almost all the varieties of bananas, especially with the emergence of <em>Foc</em> race 4. This study investigates the possibility of <em>Streptomyces rochei</em> AMBEAROOT2, isolated from the banana, used as a biocontrol agent against <em>Fusarium</em> wilt in banana. The molecular characterization of ten <em>Foc</em> isolates identified <em>Fusarium oxysporum</em> f. sp. <em>cubense</em> TFOC6 as the most virulent and confirmed as race 4. <em>S. rochei</em> AMBEAROOT2 exhibited significant mycelial inhibition (62.3%) of <em>F. oxysporum</em> f. sp. <em>cubense</em> TFOC6 and plant growth-promoting characteristics, including indole-3-acetic acid, biofilm formation, and exopolysaccharide synthesis. Gas Chromatography-Mass Spectroscopy analysis identified metabolites produced by <em>S. rochei</em> AMBEAROOT2, with molecular docking investigations revealed strong binding affinities of compounds <em>viz.,</em> 3-phenyl-3-p-tolyl-1-(2,4,6-trimethyl-phenyl)-propane-1-one, 19-acetoxy-4,4-dimethyl-, oxime, Androst-5-en-3-one, and Pyrano [4,3] benzopyran-1,9-dione to key virulence proteins (Catalase-peroxidase, Kynureninase, Penta functional AROM polypeptide, and Ribose phosphate pyrophosphokinase) of <em>Foc</em>. In greenhouse conditions, micro-propagated banana plantlets treated with <em>S. rochei</em> AMBEAROOT2 demonstrated enhanced growth, improved physiological traits, and higher levels of defense enzymes compared to those inoculated with <em>F. oxysporum</em> f. sp. <em>cubense</em> TFOC6 alone. It also induced systemic resistance and a 100% decrease in the incidence of <em>Fusarium</em> wilt. In conclusion, <em>S. rochei</em> AMBEAROOT2 showed potential antifungal activities, promoted plant growth, and could be used to manage wilt disease in banana after field evaluation.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100779"},"PeriodicalIF":6.8,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing macroalgal cell walls to trigger immunity in Arabidopsis thaliana","authors":"Jorge Peláez ,&nbsp;Carlos Frey ,&nbsp;Diego Rebaque ,&nbsp;Francisco Vilaplana ,&nbsp;Antonio Encina ,&nbsp;Hugo Mélida","doi":"10.1016/j.stress.2025.100783","DOIUrl":"10.1016/j.stress.2025.100783","url":null,"abstract":"<div><div>There is an increasing need to find sustainable alternatives to conventional agrochemicals to reduce biotic stress in crops. One possible strategy is based on promoting the innate defences of plants by stimulating their immune system. The plant immune system relies on the perception of molecules, which trigger a cascade of biochemical responses known as pattern-triggered immunity (PTI). This study investigated the potential of marine macroalgal cell wall components to be perceived by plants, act as elicitors of plant immune responses and induce disease resistance.</div><div>Cell walls of green, red, and brown algae species were chemically fractionated, and the research focused on testing their ability to induce immune responses in <em>Arabidopsis thaliana</em>. Different PTI hallmarks were tested, including H₂O₂ production, mitogen-activated protein kinases (MAPKs) phosphorylation, and defence gene expression analysis. The results showed that the CaCl₂-extracted fraction was particularly efficacious in inducing H₂O₂ production. As the CaCl₂ fraction of all phylogenetic groups also triggered additional immune responses, its ability to protect Arabidopsis against the bacterial pathogen <em>Pseudomonas syringae</em> was evaluated, confirming that certain CaCl₂ fractions successfully provided resistance to the pathogen. The monosaccharide and glycosidic linkage analysis of these fractions pointed to some specific algal cell wall glycans (e.g. porphyrans and fucoidans) that could contribute to the immunostimulatory capacity, thereby paving the way for the identification of distinct structures with potential agrobiological applications.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100783"},"PeriodicalIF":6.8,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143511675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing lodging resistance in maize: Integrating genetic, hormonal, and agronomic insights for sustainable crop productivity","authors":"Shumila Ishfaq ,&nbsp;Yi Ding ,&nbsp;Xiaoyan Liang ,&nbsp;Wei Guo","doi":"10.1016/j.stress.2025.100777","DOIUrl":"10.1016/j.stress.2025.100777","url":null,"abstract":"<div><div>Crop lodging, characterized by the bending or breaking of plant stems, poses a significant challenge to global food security by reducing crop yields and complicating harvesting processes. This review explores the factors influencing lodging susceptibility, including environmental conditions, genetic traits, fertilizer management, pathogens, and hormonal regulation. Recent advancements in maize research have uncovered critical genetic traits and elucidated the roles of key hormonal pathways—such as gibberellin (GA), strigolactone (SL), auxin, and ethylene—in modulating stem elongation, tillering angles, and root system architecture. These pathways collectively shape crop architecture, with GA and SL contributing to stalk strength, and auxin and ethylene enhancing root development and plant stability. Concurrently, agronomic interventions, such as optimized planting density and nutrient management, have improved stem integrity and mitigated lodging risk. By integrating genetic, hormonal, and agronomic knowledge, researchers have made remarkable progress in developing maize varieties that resist lodging, enhancing crop resilience and yield stability under various environmental conditions. Future research should focus on unraveling the molecular and genetic mechanisms underlying lodging resistance, addressing technical limitations in implementation, and advancing sustainable agricultural practices to secure global food production and ensure long-term productivity.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100777"},"PeriodicalIF":6.8,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143463334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNA-Seq-based analysis of transcriptomic signatures elicited by mutations conferring salt tolerance in Cucurbita pepo","authors":"Keshav Gautam,&nbsp;Sonsoles Alonso,&nbsp;Alicia García,&nbsp;María Segura,&nbsp;Álvaro Benítez,&nbsp;Cecilia Martínez,&nbsp;Manuel Jamilena","doi":"10.1016/j.stress.2025.100775","DOIUrl":"10.1016/j.stress.2025.100775","url":null,"abstract":"<div><div>Salinity is a major determinant of plant growth and crop productivity, resulting in significant economic losses in agriculture. Improving salinity tolerance in plant breeding programs requires not only donor tolerant genotypes but also a thorough knowledge of the genes controlling the trait. Taking advantage of two recently identified salinity-tolerant EMS mutants of squash (<em>sal-1</em> and <em>sal-2</em>), this study aimed to analyse whether these two sources of salt tolerance are associated with similar transcriptomic changes in leaves. RNA sequencing revealed that the two mutants have a very distinct transcriptomic response to salt stress compared to the WT, with 154 and 1068 salt-tolerance-associated differentially expressed genes (DEGs) in <em>sal-1</em> and <em>sal-2</em>, respectively. GO and KEGG enrichment analyses revealed the importance of several phytohormone biosynthesis, signalling and transport genes (<em>CpAUX22B/22D, CpSAUR32–2, CpARR5/12, CpAHK2/3, CpBZR1, CpTCH4, CpNCED1, CpCYP707A1, CpPP2C, CpSnRK1/2, CpLOX2</em> and <em>CpACX</em>) in the salt tolerance response. MAPK genes (<em>CpMPK3</em> and <em>CpMEKK1</em>) and the Ca²⁺ signalling network (<em>CpCPK26/28/34, CpCML31/36/48, CpPBP1, CpCBL1</em> and <em>CpRBOHD</em>) were also specifically activated in salt-tolerant mutants, indicating their contribution to salt tolerance. Genes for antioxidant enzymes (PP2, POD, CAT, PRX, GST and GRX) and cell wall metabolism were also up-regulated in salt-tolerant mutants, reducing oxidative stress and maintaining the integrity of membranes and other cellular structures. Genes for ion transporters were significantly up-regulated in response to salt stress in <em>sal-2</em>, probably involved in maintaining ion homeostasis. Several genes encoding transcription factors of the ERF, C3H, Dof, HD-ZIP, MYB, HSF, NAC, knotted and WRKY families, as well as long non-coding RNA, were also found to positively or negatively regulate salt stress tolerance in the <em>sal-1</em> and <em>sal-2</em> mutants. Overall, the results highlight the complexity of the molecular response involved in salt stress tolerance in <em>C. pepo</em> and prioritise further investigation of specific genes that contribute to the resilience of crops under saline conditions.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100775"},"PeriodicalIF":6.8,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morpho-physiological and transcriptomic analyses reveal adaptive responses of Neopyropia yezoensis to long-term high temperature","authors":"Tian Gao ,&nbsp;Xianghai Tang ,&nbsp;Dongmei Wang ,&nbsp;Yahui Yu ,&nbsp;Yunxiang Mao","doi":"10.1016/j.stress.2025.100778","DOIUrl":"10.1016/j.stress.2025.100778","url":null,"abstract":"<div><div>High temperature has a significant impact on growth and yield of <em>Neopyropia yezoensis</em>, and its effects are expected to increase in the near future due to global warming. Thus, understanding the high-temperature response of <em>N. yezoensis</em> and enhancing its ability to adapt to high temperature have become of critical importance to aquaculture development. In this study, we investigated the changes in morphology, physiology and transcriptome of a high-temperature tolerant strain of <em>N. yezoensis</em> under high temperature to explore its response mechanism to high temperature. Notable changes have been observed in thallus color, relative growth rates, pigment composition, chlorophyll fluorescence, ROS levels and gene expression. Our results suggested that thallus color change was one of the most important adaptive responses of <em>N. yezoensis</em> to long-term high temperature, and was reported for the first time in our present study. Moreover, accumulation of phycoerythrin could confer high temperature tolerance to <em>N. yezoensis</em>. Finally, combining our data with previous reports, we proposed a model of possible mechanisms of <em>N. yezoensis</em> adapting to long-term high temperature. Our findings provide new insights into the adaptative mechanisms of <em>N. yezoensis</em> to high temperature environments, which could be helpful for improving the high-temperature tolerance of <em>N. yezoensis</em> and other economic algae in the aquaculture industry.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100778"},"PeriodicalIF":6.8,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ApHKT1 confers salinity tolerance in Apocynum by restraining the intake of Na+/K+in root tissues","authors":"Haohan Zhao ,&nbsp;Xiaoyu Huang ,&nbsp;Yue Wang ,&nbsp;Aiguo Zhu ,&nbsp;Xiaofei Wang ,&nbsp;Hanipa Hazaisi ,&nbsp;Gang Gao ,&nbsp;Li Jiang ,&nbsp;Jikang Chen","doi":"10.1016/j.stress.2025.100776","DOIUrl":"10.1016/j.stress.2025.100776","url":null,"abstract":"<div><div><em>Apocynum</em>, exhibiting tolerance to severe salt stress, provides important materials for textile, medicine and biofuels. However, the underlying mechanism of <em>Apocynum</em> adapting to salt stresses remains poorly understood. In this study, physiological methods combined with bioinformatics tools were employed to insight the mechanism of <em>Apocynum</em> responding to salt stresses. Three representative species, <em>A. hendersonii, A. venetum</em> and tetraploid of <em>A. venetum</em> and NaCl treatments ranges from 50 mM to 400 mM were compared to reveal a comprehensive profile of the plants under salt stresses. As a crucial phenotypic characteristic for selecting plants that exhibit resilience to salinity and drought stress, the root-to-shoot ration of <em>Apocynum</em> was increased significantly while the growth of <em>Apocynum</em> was markedly inhibited as the degree of salt stress intensifying. The stomatal apertures on the leaf epidermis of <em>Apocynum</em> significantly narrowed in response to salt stress, and the chlorophyll content exhibited an overall declining trend. Salt stress notably elevated the Na⁺ and K⁺ content in the roots, stems, and leaves of <em>Apocynum</em>, with a significant decrease in the K⁺/Na⁺ ratio, while <em>A. hendersonii</em> showing the greatest change in this ratio. Phenotypic analysis indicated that <em>A. hendersonii</em> possessed the strongest salt tolerance among the species. ApHKT1, the highly conservative protein in the three species which primarily expressed in roots were hypothesized to adapt to salt stress by regulating the transportation of Na⁺ and K⁺. Although the content of Na⁺ and K⁺were increased in stem and leaf, there was no significant accumulation of Na⁺ and K⁺in root tissues. Expression pattern analysis found that <em>ApHKT1</em> were significantly down-regulated under the rising salt stress in the root. These results suggested that <em>Apocynum</em> mainly take the strategy of reducing of <em>ApHKT1</em> expression and the Na⁺/K⁺ intake to maintain the ion balance under salt stress.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100776"},"PeriodicalIF":6.8,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide analysis of the C2H2-type zinc finger protein family in rice (Oryza sativa) and the role of OsC2H2.35 in cold stress response","authors":"Songguo Wu ,&nbsp;Yuzhang Chen ,&nbsp;Jianguo Li ,&nbsp;Chunli Fu ,&nbsp;Xiaoying Luo ,&nbsp;Jingzhen Wang ,&nbsp;Xincheng Wan ,&nbsp;Ke Huang ,&nbsp;Hailian Zhou ,&nbsp;Guosheng Xie ,&nbsp;Zhengdan Wu ,&nbsp;Lingqiang Wang","doi":"10.1016/j.stress.2025.100772","DOIUrl":"10.1016/j.stress.2025.100772","url":null,"abstract":"<div><div>Cold can be a tough challenge for rice cultivation, impacting its growth and overall productivity. The Cys2His2 (C2H2) zinc finger (ZF) genes are essential for plants’ responses to abiotic stress. In this study, we identified 99 <em>OsC2H2</em> genes within the <em>Oryza sativa japonica</em> genome, detailing their gene structure, conserved C2H2-ZF domains, and motif compositions for the first time. We also examined the temporal expression patterns of these genes under cold, heat, drought, flooding, and salt stress. Interestingly, we found that <em>OsC2H2.35</em> was upregulated during cold stress, and CRISPR/Cas9 editing of this gene enhances rice cold tolerance in seedlings. RNA-seq results showed that OsC2H2.35 negatively regulates several <em>COR</em> genes, including <em>DEHYDRATION-RESPONSIVE ELEMENT BINDING FACTORS 1</em> <em>s</em> (<em>OsDREB1A, OsDREB1B</em>, and <em>OsDREB1C</em>). Specifically, OsC2H2.35 can directly bind to the promoters of <em>OsDREB1A</em> and <em>OsDREB1C. Osc2h2.35</em> greatly enhances cold tolerance while preserving all essential agronomic traits, making it a valuable gene target for the genetic improvement of rice.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100772"},"PeriodicalIF":6.8,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}