Plant Stress最新文献_第7页

Untapped potential of calcium and nano-calcium to develop abiotic stress resilience in photosynthetic machinery: The primary source of plant food and fuels

IF 6.8

Plant Stress Pub Date : 2024-12-17 DOI: 10.1016/j.stress.2024.100718

Mohammad Faizan , Bhavya Somaplara Gangadharappa , Pravej Alam , Sadia Haque Tonny , Katenahalli Rudrappa Maruthi , Shamsul Hayat

{"title":"Untapped potential of calcium and nano-calcium to develop abiotic stress resilience in photosynthetic machinery: The primary source of plant food and fuels","authors":"Mohammad Faizan , Bhavya Somaplara Gangadharappa , Pravej Alam , Sadia Haque Tonny , Katenahalli Rudrappa Maruthi , Shamsul Hayat","doi":"10.1016/j.stress.2024.100718","DOIUrl":"10.1016/j.stress.2024.100718","url":null,"abstract":"<div><div>Photosynthesis is a special mechanism that has formed life on earth and created the conditions for all known life. The function of calcium (Ca) as a secondary messenger in plants has been the subject of substantial research in recent decades. Due to their sessile nature, plants are subject to a variety of abiotic stresses, such as pesticide pollution, heavy metals, salt, drought, nutrient deficiencies, light intensity, and severe temperatures. Abiotic stresses mainly lower plants' photosynthetic efficiency because they have detrimental effects on gas exchange parameters, electron transport processes, photosystem performance, and chlorophyll production. The decline in photosynthetic capacity of plants due to these stresses is directly associated with reduction in yield. Therefore, detailed information of the role of calcium (Ca) and nano-Ca on photosynthetic machinery and better understanding of the photosynthetic machinery could help in developing new strategies with higher yield even under stressed environments. Interestingly, in this review, we provide an overview of insight into mechanism affecting photosynthesis under abiotic stresses. The present review explains how several abiotic stressors can negatively affect the photosynthesis mechanism and Ca and nano-Ca mediated-regulation in plant photosynthesis. The review also highlights the advantages of using nano-Ca to increase photosynthetic efficiency.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100718"},"PeriodicalIF":6.8,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143098943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the deleterious effects of heavy metal cadmium on antioxidant defense and photosynthetic pathways in higher plants

IF 6.8

Plant Stress Pub Date : 2024-12-16 DOI: 10.1016/j.stress.2024.100716

Jinxiao Song , Zhaomei Sun , Shah Saud , Shah Fahad , Taufiq Nawaz

{"title":"Exploring the deleterious effects of heavy metal cadmium on antioxidant defense and photosynthetic pathways in higher plants","authors":"Jinxiao Song , Zhaomei Sun , Shah Saud , Shah Fahad , Taufiq Nawaz","doi":"10.1016/j.stress.2024.100716","DOIUrl":"10.1016/j.stress.2024.100716","url":null,"abstract":"<div><div>Cadmium (Cd) is a highly toxic heavy metal and a major inorganic pollutant in soil ecosystems. Due to its high mobility and solubility, plants easily absorb Cd, affecting their physiological and biochemical processes, crop quality, and ultimately human health through bioaccumulation in the food chain. This review provides a comprehensive analysis of recent advances in understanding Cd toxicity in soil. It studies the influence of Cd on plant growth and development, focusing on disruptions in physiological and biochemical processes, changes in cellular ultrastructure, changes in biomass accumulation, and changes in nutritional quality. The review summarizes current findings on the mechanisms of Cd-induced toxicity, particularly its effects on antioxidant and photosynthetic systems. The broader ecological consequences of Cd contamination on ecosystem health and biodiversity are also examined. In addition, the article discusses new phytoremediation and genetic engineering strategies aimed at increasing plant resistance to Cd stress. Future research directions are suggested to address existing knowledge gaps and improve remediation efforts.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100716"},"PeriodicalIF":6.8,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143098248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zinc-chitosan nanocomposites as guardians against the dreaded phytopathogenic fungus Macrophomina phaseolina in Vigna radiata L.

IF 6.8

Plant Stress Pub Date : 2024-12-16 DOI: 10.1016/j.stress.2024.100710

Uswa Fatima , Amna Shoaib , Qudsia Fatima , Abdulaziz Abdullah Alsahli , Parvaiz Ahmad

{"title":"Zinc-chitosan nanocomposites as guardians against the dreaded phytopathogenic fungus Macrophomina phaseolina in Vigna radiata L.","authors":"Uswa Fatima , Amna Shoaib , Qudsia Fatima , Abdulaziz Abdullah Alsahli , Parvaiz Ahmad","doi":"10.1016/j.stress.2024.100710","DOIUrl":"10.1016/j.stress.2024.100710","url":null,"abstract":"<div><div><em>Macrophomina phaseolina,</em> a phytopathogenic fungus responsible for root rot in mung beans (<em>Vigna radiata</em> L.), produces resilient sclerotia that are not effectively managed by chemical fungicides. In this study, as an alternative management approach, zinc-chitosan nanoparticles (Zn-ChNPs) were prepared using the ionic gelation method and evaluated for their antifungal activity against <em>M. phaseolina.</em> The synthesis of Zn-ChNPs was confirmed by UV–visible spectroscopy with absorption peaks at 215 nm and 265 nm. XRD indicated hexagonal crystalline planes, verifying nanoparticle crystallinity, while FTIR showed strong ZnO-chitosan interactions with peaks at 3495 cm⁻¹ and 678 cm⁻¹. The particles averaged 80–100 nm in size<em>.</em> Antifungal bioassays demonstrated significant inhibition of fungal growth, achieving 50–100 % reduction at concentrations of 0.11 % and above, and an EC<sub>50</sub> (effective concentration) value of 0.08 %. Microscopic analysis revealed sclerotia distortion at 0.15 % Zn-ChNPs, while enzymatic assays showed a 20–60 % increase in catalase, peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase activities at concentrations of 0.03–0.11 %, followed by a sharp decrease beyond 0.11 %. <em>In planta</em> bioassays indicated that 0.4–0.6 % Zn-ChNPs reduced disease by 97 % and improved growth up to 100 %, surpassing the performance of chemical fungicides (Carbendazim). Multivariate analysis further underscored the superior efficacy of Zn-ChNPs in enhancing plant defense mechanisms and managing root rot disease. These findings highlighted the potential of Zn-ChNPs as a sustainable and effective alternative to chemical fungicides, offering dual benefits of disease control and growth enhancement in mung bean plants.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100710"},"PeriodicalIF":6.8,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143097828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Root colonizing microbes associated with notable abiotic stress of global food and cash crops

IF 6.8

Plant Stress Pub Date : 2024-12-15 DOI: 10.1016/j.stress.2024.100714

Oghoye Priscilla Oyedoh , Stéphane Compant , Sharon L. Doty , Gustavo Santoyo , Bernard R. Glick , Olubukola Oluranti Babalola

{"title":"Root colonizing microbes associated with notable abiotic stress of global food and cash crops","authors":"Oghoye Priscilla Oyedoh , Stéphane Compant , Sharon L. Doty , Gustavo Santoyo , Bernard R. Glick , Olubukola Oluranti Babalola","doi":"10.1016/j.stress.2024.100714","DOIUrl":"10.1016/j.stress.2024.100714","url":null,"abstract":"<div><div>The increase in cash crop productivity has helped improve farmers, communities, and economic streams and has served as a source of income. However, the erratic global climate change deeply affects crops' productivity due to the exacerbating high temperatures predominant in some geographical regions, which has translated into other environmental issues. Combinatorial impacts of these climate-associated problems have exposed agricultural lands to multiple abiotic stresses, placing massive pressure on cultivatable arable lands and reducing crop productivity. Agronomists, through an understanding of single abiotic stressors, have innovated new concepts like nanotechnology, molecular modified and cross-bred varieties to unravel these problems. However, the complexity, toxicity, and resistance development associated with these approaches have consistently left the search window open for novel, natural, and more sustainable alternatives. To this end, the roles of root microbiomes, particularly root endophytes, in ensuring plant growth promotion through siderophore production, phytohormone release and regulation, and nutrient fertilization have been harnessed in an effort to resolve single, double, and multiple abiotic stressors without a complete understanding of the pathways involved in each mechanism of plant growth promotion. Thus, to successfully leverage root endophytes for growth promotion during multi-stresses, this review is targeted to reveal single abiotic stress mechanism for each cash crop group, as this could help conceptualize the details involved in stress control and the alleviation of multi-stresses through nutrient fertilization pathways.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100714"},"PeriodicalIF":6.8,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143445701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PsDUF6A from Populus simonii enhances drought tolerance in transgenic Arabidopsis and poplar by increasing ROS scavenging

IF 6.8

Plant Stress Pub Date : 2024-12-13 DOI: 10.1016/j.stress.2024.100706

Yanfei Yang , Jinna Zhao , Xingrong Ren , Xueqi Bai , Tao Li , Jianbo Li

{"title":"PsDUF6A from Populus simonii enhances drought tolerance in transgenic Arabidopsis and poplar by increasing ROS scavenging","authors":"Yanfei Yang , Jinna Zhao , Xingrong Ren , Xueqi Bai , Tao Li , Jianbo Li","doi":"10.1016/j.stress.2024.100706","DOIUrl":"10.1016/j.stress.2024.100706","url":null,"abstract":"<div><div>Domain of unknown function (DUF) proteins play roles in a range of plant biological processes, including growth and development, and adaptation to abiotic stresses. However, their function was largely unknown in woody plants. <em>Populus simonii</em> is a notable native tree species in northern China and is highly tolerance to drought stress. In this study, <em>PsDUF6A</em> was isolated and functionally characterized from <em>P. simonii</em>. This gene was highly expressed in mature leaves and its expression was induced under drought condition. Transgenic <em>Arabidopsis</em> and 84 K poplar lines overexpressing <em>PsDUF6A</em> were constructed to investigate the function of <em>PsDUF6A</em> in drought tolerance. Under drought conditions, the survival rate and relative water content were higher in <em>PsDUF6A</em>-overexpressing <em>Arabidopsis</em> than in wild-type <em>Arabidopsis</em>, whereas the opposite trend was observed for relative electrical conductivity, indicative of increased drought tolerance. Compared with 84 K poplar, transgenic poplar had a higher photosynthetic activity, lower water loss rate, and higher root biomass. Moreover, <em>PsDUF6A-</em>overexpressing increased antioxidant enzyme activities and the reactive oxygen species scavenging. In addition, the yeast one-hybrid assay indicated that PsC2H213, PsC2H214, PsC2H215, PsC2H217, and PsC2H218 can directly bind to <em>PsDUF6A</em> promoter. These results indicated that <em>PsDUF6A</em> enhances drought tolerance by maintaining ROS homeostasis, and its expression might regulate by C2H2-type ZFPs. These findings revealed the positive contributions of <em>PsDUF6A</em> to drought tolerance and provided insights into the underlying regulatory network of <em>P. simonii</em> response to drought stress.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100706"},"PeriodicalIF":6.8,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143098247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mapping QTLs for aluminium and phosphorus tolerances at seedling and reproductive stages in lentil (Lens culinaris Medikus)

IF 6.8

Plant Stress Pub Date : 2024-12-12 DOI: 10.1016/j.stress.2024.100709

Noren Singh Konjengbam , Dharmendra Singh , Anima Mahato , Vikram Jeet Singh , Jyoti Taunk

{"title":"Mapping QTLs for aluminium and phosphorus tolerances at seedling and reproductive stages in lentil (Lens culinaris Medikus)","authors":"Noren Singh Konjengbam , Dharmendra Singh , Anima Mahato , Vikram Jeet Singh , Jyoti Taunk","doi":"10.1016/j.stress.2024.100709","DOIUrl":"10.1016/j.stress.2024.100709","url":null,"abstract":"<div><div>Aluminium toxicity and phosphorus deficiency are primary limitations to crop yield under acidic soil conditions. Developing aluminium (Al) and phosphorous (P) stress tolerant cultivars is one of the effective way to increase plant productivity under acidic soils. Hence, the present study was conducted at 6 different acidic environments during 2020–21 and 2021–22 to map quantitative trait loci (QTLs) associated with Al and low P tolerances in 150 F<sub>7</sub> recombinant inbred lines derived from BM-4 (Al-sensitive) x L-4602 (Al-tolerant) genotypes. Sixteen morpho-physiological and yield related traits were studied in response to Al and P stresses under hydroponic and field conditions. Seven QTLs were identified using composite interval mapping on linkage group 1 for six morpho-physiological traits including root re-growth (RRG), fluorescent signals (callose accumulation), aluminium content, phosphorus content, days to flowering and days to maturity under Al stress (E-1), Al with P stresses (E-2) and acidic field condition (E-6). Individual QTLs accounted for logarithm of odd (LOD) values of 2.73 to 6.85 and phenotypic variation between 5.4 % to 19.8 %. The major QTLs with Al tolerance and P efficiency components can be integrated into elite lentil cultivars using molecular breeding. Improved lentil lines for Al tolerance and P efficiency will reduce the input need for lime application and phosphate fertilizers, significantly cutting costs, together with increased productivity in acidic, and P deficient soils, altogether boosting farmers’ and seed industry profitability.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100709"},"PeriodicalIF":6.8,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143098940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanoencapsulated nitric oxide donor triggers a dose-dependent effect on the responses of maize seedlings to high light stress

IF 6.8

Plant Stress Pub Date : 2024-12-12 DOI: 10.1016/j.stress.2024.100711

Diego G. Gomes , Bruno T. Sousa , Joana C. Pieretti , Roney H. Pereira , Wagner R. de Souza , Halley C. Oliveira , Amedea B Seabra

{"title":"Nanoencapsulated nitric oxide donor triggers a dose-dependent effect on the responses of maize seedlings to high light stress","authors":"Diego G. Gomes , Bruno T. Sousa , Joana C. Pieretti , Roney H. Pereira , Wagner R. de Souza , Halley C. Oliveira , Amedea B Seabra","doi":"10.1016/j.stress.2024.100711","DOIUrl":"10.1016/j.stress.2024.100711","url":null,"abstract":"<div><div>Nanoencapsulation of nitric oxide (NO) donors provides sustained release of NO, prolonging its action on plants. Here, we evaluated the action of NO-releasing chitosan nanoparticles containing GSNO (S-nitrosoglutathione) on the protection of maize plants (<em>Zea mays</em> cv. Balu 787) against high light-induced stress. Experiment 1 was used to compare maize plants under different light intensities. Experiment 2 evaluated the protective effect of chitosan nanoparticles containing GSNO (NPNO) in different concentrations (200 or 400 µM). Experiment 3 compared the protective effect of NPNO to non-nanoencapsulated GSNO (NO) and nanoparticles without NO-releasing molecule (NP). In experiments 1 and 2, chlorophyll <em>a</em> fluorescence and gas exchange measurements were performed. In experiment 3, chlorophyll <em>a</em> fluorescence and biochemical analyses were carried out. In experiment 1, increases in dynamic photoinhibition (DP) of 135 % (Day 1), 370 % (Day 2), 206 % (Day 3), and 100 % (Day 5) were observed from sun plants. In experiment 2, NPNO400 showed higher levels of DP on the first (+ 148 %) and second days (+ 171 %), followed by a reduction on the fifth day (- 22 %). For gas exchange parameters, NPNO400 attenuated the reduction in <em>A</em> at noon and significantly increased <em>k</em>, while NPNO200 decreased the <em>k</em> value. The differences in the effects induced by NPNO treatments are dose-dependent. In experiment 3, NPNO was the only treatment that significantly increased NO bioavailability and the activity of antioxidant enzymes (SOD; POD), contributing to mitigating stress caused by excess light on plants. The nanoencapsulation of NO donors protected maize plants against photoinhibition.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100711"},"PeriodicalIF":6.8,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143097827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering the genomic regions associated with seedling cold tolerance traits in rice (Oryza sativa L.)

IF 6.8

Plant Stress Pub Date : 2024-12-12 DOI: 10.1016/j.stress.2024.100707

Gurjeet Singh , Anjan Kumar Pradhan , Subroto Das Jyoti , Chersty L. Harper , Punniakotti Elumalai , Darlene L. Sanchez , Stanley Omar PB. Samonte , Shyamal K. Talukder

{"title":"Deciphering the genomic regions associated with seedling cold tolerance traits in rice (Oryza sativa L.)","authors":"Gurjeet Singh , Anjan Kumar Pradhan , Subroto Das Jyoti , Chersty L. Harper , Punniakotti Elumalai , Darlene L. Sanchez , Stanley Omar PB. Samonte , Shyamal K. Talukder","doi":"10.1016/j.stress.2024.100707","DOIUrl":"10.1016/j.stress.2024.100707","url":null,"abstract":"<div><div>Seedling cold tolerance (SCT) in rice is important for planting rice in colder temperatures that occur during early planting and ratooning in some rice-growing regions in the world. Genome-wide association study (GWAS) has the potential to understand the genetic mechanisms of complex traits like SCT. A set of 204 rice accessions were screened for SCT traits in three environments: natural cold conditions (E1; temperature 6.3 °C-23.3 °C), growth chamber cold conditions (E2; 10 °C in 1st and 2nd weeks followed by 17 °C in 3rd and 4th weeks), and normal growth condition in the greenhouse (E3; day and night temperature maintained at 28–30 °C). Data collected on the number of emerged seedlings at six, eight, and twelve days after sowing, and seedling length (SL) was measured at two and four weeks after sowing. GWAS analysis identified nine quantitative trait nucleotides (QTNs) with phenotypic variation ranging from 10.98 to 20.72%. Among them, S06_22947376, S07_27594541, and S07_3833577 showed pleiotropic responses for multiple traits in different experiments. Candidate gene analysis of S06_22947376 identified four putative genes, i.e., <em>Os06g0585950, Os06g0585982, Os06g0586150</em>, and <em>Os06g0587200</em> around the region to be associated with a protein kinase responsible for increasing the SCT. The results of this study provide valuable information for understanding the genetic control of SCT and the further development of molecular markers that are useful for breeding programs for the development of rice cultivars tolerant to cold stress. At the same time, rice accessions showing potential SCT will be integrated into the breeding program for varietal development.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100707"},"PeriodicalIF":6.8,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143098245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined transcriptome and metabolome analysis provides insight into the ERF073 – Malic acid network in pakchoi under submergence stress

IF 6.8

Plant Stress Pub Date : 2024-12-12 DOI: 10.1016/j.stress.2024.100708

Dan-dan Xi , Lu Gao , Li-ao Ge , Hong-fang Zhu , Li-ming Miao , Ding-yu Zhang , Chang-wei Zhang , Ying Li , Yan-xiao Dong , Xi-lin Hou , Yu-ying Zhu , Zhao-hui Zhang , Xiao-feng Li

{"title":"Combined transcriptome and metabolome analysis provides insight into the ERF073 – Malic acid network in pakchoi under submergence stress","authors":"Dan-dan Xi , Lu Gao , Li-ao Ge , Hong-fang Zhu , Li-ming Miao , Ding-yu Zhang , Chang-wei Zhang , Ying Li , Yan-xiao Dong , Xi-lin Hou , Yu-ying Zhu , Zhao-hui Zhang , Xiao-feng Li","doi":"10.1016/j.stress.2024.100708","DOIUrl":"10.1016/j.stress.2024.100708","url":null,"abstract":"<div><div>Pakchoi is sensitive to oxygen deficiency caused by submergence; however, the molecular mechanisms underlying its response to these conditions remains unclear. To determine these mechanisms, two pakchoi cultivars, Heiyoudong (HYD) and Suzhouqing (SZQ), were subjected to submergence for 1 h and 5 h, respectively. Following this treatment, the plants were transferred back to the control conditions for recovery. HYD exhibited higher survival rate than SZQ after recovery. Leaves from 1 h stress, 5 h stress, and subsequent 5-h recovery phases were harvested for metabolome and transcriptome analyses. Data analysis revealed that 206 differentially accumulated metabolites (DAMs) were identified between SZQ and HYD after 1 h stress (S1 vs H1) and 330 DAMs were identified between SZQ and HYD after 5 h stress (S5 vs H5) at stress stage. During recovery stage, 124 and 310 DAMs were found in S1 vs H1 and S5 vs H5, respectively. Additionally, 553 commonly differentially expressed genes (DEGs) were found both in S1 vs H1 and S5 vs H5 at the stress stage, while 458 DEGs were commonly found in the two comparison groups at the recovery stage. Among these DEGs, ERF073 was down-regulated, as further confirmed by qRT-PCR. KEGG pathway analysis revealed that DAMs and DEGs were mainly enriched in metabolic pathways. Taken together, these findings indicate that ERF073 may regulate malic acid accumulation in pakchoi, increasing its resistance to submergence.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100708"},"PeriodicalIF":6.8,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143098941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiological response and transcriptomic analysis of red-fleshed apple seedlings to low temperature stress

IF 6.8

Plant Stress Pub Date : 2024-12-11 DOI: 10.1016/j.stress.2024.100696

LingYu Meng , Rui Zhang , YanPing Wei , Tingting Gu , Jiahao Zhao , Zijing Chen , Zongying Zhang , Nan Wang , Xuesen Chen , Wenjun Liu

{"title":"Physiological response and transcriptomic analysis of red-fleshed apple seedlings to low temperature stress","authors":"LingYu Meng , Rui Zhang , YanPing Wei , Tingting Gu , Jiahao Zhao , Zijing Chen , Zongying Zhang , Nan Wang , Xuesen Chen , Wenjun Liu","doi":"10.1016/j.stress.2024.100696","DOIUrl":"10.1016/j.stress.2024.100696","url":null,"abstract":"<div><div>Low temperature (LT) stress negatively impacts the yield and quality of key horticultural crops such as apples. While previous research has predominantly focused on white-fleshed apple varieties with limited cold tolerance, the mechanisms underlying low temperature responses in red-fleshed apples remain largely unexplored. In this study, we used red-fleshed apple seedlings that we developed previously to investigate the biochemical responses to varying temperature conditions. Notably, LT stress significantly enhanced the accumulation of anthocyanins, soluble sugars, soluble proteins, and other metabolites in the red-fleshed apple seedlings. Leveraging RNA-seq data, we identified a potential LT stress-responsive transcription factor, which we designated as MdbHLH51. Functional characterization revealed that overexpression of <em>MdbHLH51</em> in ‘Orin’ calli significantly promoted anthocyanin accumulation and up-regulated the expression of all <em>MdCBFs</em> genes, thereby bolstering tolerance to cold stress. These findings provide valuable insights into the cold stress response mechanisms in red-fleshed apples, offering a theoretical foundation for the genetic breeding of cold-resistant red apple varieties.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100696"},"PeriodicalIF":6.8,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143097826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0