Plant Stress最新文献

{"title":"Recover and surpass: The mechanisms of plants transition upon rehydration from drought","authors":"Xubo Ke ,&nbsp;Jia Yao ,&nbsp;Zhihan Jiang,&nbsp;Xinyue Gu,&nbsp;Pei Xu","doi":"10.1016/j.stress.2025.100782","DOIUrl":"10.1016/j.stress.2025.100782","url":null,"abstract":"<div><div>As the global climate shifts and precipitation patterns evolve, the drought resistance of plants is being increasingly tested. There is a notable variation in the post-drought rehydration recovery capabilities among different plant species, which significantly influences both plant growth and the regulation of ecological environments. Researchers have achieved considerable advancements in the field of drought resistance; however, our comprehension of the processes involved in post-drought rehydration remains limited. Rehydration following a drought event is crucial not only for compensating plant growth but also for facilitating the recovery of terrestrial ecosystems. This paper begins by reviewing the current state of research on plant drought resistance and then delves into the progress and associated strategies for post-drought rehydration. Identifying the existing gaps in research, we propose potential directions for future studies to provide insights into how plants respond to drought and rehydration under changing climatic conditions. This collection of information is valuable to enhance our understanding of the rehydration mechanisms post-drought and to lay a theoretical foundation for the mining of rehydration genes and the improvement of drought-resistant plant varieties in the future.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100782"},"PeriodicalIF":6.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143535111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping spatial heterogeneity of non-structural carbohydrates in Haloxylon ammodendron using remote sensing in extreme desert environments","authors":"Weiyi Zhou ,&nbsp;Jing Zhang ,&nbsp;Benfeng Yin ,&nbsp;Lan Peng ,&nbsp;Lingyue Wang ,&nbsp;Xiaobing Zhou ,&nbsp;Yaoli Zhou ,&nbsp;Yanfeng Di ,&nbsp;Hongwei Zheng ,&nbsp;Yuanming Zhang","doi":"10.1016/j.stress.2025.100790","DOIUrl":"10.1016/j.stress.2025.100790","url":null,"abstract":"<div><div>Arid regions occupy 40 % of the Earth's land surface and play a significant role in the global carbon budget. Dominant desert shrub <em>Haloxylon ammodendron</em>, which thrives under extreme climatic conditions, contributes substantially to the stability of desert ecosystems. However, its species distribution patterns and carbon spatial dynamics in deserts remain unknown. Based on our newly development “Satellite-Airborne-Field” Ensemble learning platform for Systematic plant CArbohydrate estimation in Nature (SAFESCAN), we accurately estimated the spatial patterns of non-structural carbohydrate (NSC) content in leaves (LNSC) and branches (BNSC), with <em>R</em>² values of 0.83 and 0.72, respectively. This analysis incorporated 51 environmental variables and 564 samples collected from 81 subplots across multiple temperate deserts between 75°E and 115°E. The results identified the northern Gurbantunggut Desert and western edges of the Taklamakan Desert in Xinjiang as primary distribution areas for <em>H. ammodendron</em>, with northern Inner Mongolia and northern Qinghai also suitable for its survival. LNSC showed a west-to-east decreasing trend, while BNSC exhibited an increasing trend. The SAFESCAN platform demonstrated strong predictive capacity for shrub-dominated arid regions but may overestimate the relationship between NSC content and certain environmental variables. By integrating macro-scale species distribution patterns and local individual patterns of <em>H. ammodendron</em>, SAFESCAN provides NSC content maps, laying the foundation for future efforts in desert plant conservation and cultivation.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100790"},"PeriodicalIF":6.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143563234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the regulatory mechanisms of morphogenesis in grape berries with different fruit shapes based on transcriptome","authors":"Xintong Nan,&nbsp;Ming Xie,&nbsp;Wenfang Li,&nbsp;Lizhen Chen,&nbsp;Shan Jiang,&nbsp;YanE Zhao,&nbsp;Zonghuan Ma","doi":"10.1016/j.stress.2025.100792","DOIUrl":"10.1016/j.stress.2025.100792","url":null,"abstract":"<div><div>Fruit shape is one of the important appearance quality, which directly affects the choice of consumers, so it is of great significance to study the formation mechanism of appearance shape for improving grapevines appearance quality. In this study, three grapevine varieties, namely 'Rizamat', 'Victoria', and 'Fujiminori', exhibiting distinct grapevine shapes, were selected as the research subjects. Transcriptome sequencing analysis was conducted on key stages of grapevines morphogenesis, while the endogenous hormone content in berries at different developmental stages was also determined. The results showed that there was no significant difference in vertical and horizontal diameters between the three berry varieties at the young fruit stage and the veraison, and the difference in vertical and horizontal diameters of grapevine at the enlargement stage was the most significant among the three grapevine varieties. At the fruit enlargement stage, the longitudinal diameter of 'Rizamat' reached 25.0 mm, followed by 'Victoria' (22.9 mm), and 'Fujiminoru' was the smallest (18.2 mm). The transverse diameter of 'Victoria' was 17.4 mm and 'Fujiminoru' was 16.1 mm, which was significantly higher than that of 'Rizamat' (14.9 mm), and the shape index showed that 'Rizamat'&gt; 'Victoria'&gt; 'Fujiminoru'. A total of 2288 differential genes were obtained by transcriptome sequencing of the pulp 25 days after anthesis, and KEGG was significantly enriched in the \"plant hormone signal transduction\" pathway, including 13 auxin signaling pathways, 2 cytokinin signaling pathways, 2 abscisic acid signaling pathways, 1 brassinolide signaling pathway, and 2 differentially expressed genes related to methyl jasmonate signaling pathway. A total of 1520 differentially expressed transcription factors were recorded, including 115 and 112 AP2/ERF-ERF and bHLH, respectively. In addition, the oblong variety 'Rizamat' was significantly higher than in the other two varieties. The contents of IAA, GA3 and ZT were significantly correlated with differential gene <em>Vitis_vinifera_newGene_3009, SAUR71-like, TIFY9</em> and <em>VIT_14s0030g02310</em>. In conclusion, the difference in the expression of gene expression related to plant hormone signal transduction regulates the difference in ZT and GA3 accumulation in fruits, which is one of the reasons for the morphological differences of 'Rizamat', 'Victoria' and 'Fujiminori'.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100792"},"PeriodicalIF":6.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143593400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-level approach to screen tomato inbred lines for resilience to Ni-enriched soils and water deficit","authors":"Daniela Fortini ,&nbsp;Roberto Chignola ,&nbsp;Giulio Zanni ,&nbsp;Alice Brunazzi ,&nbsp;Anita Zamboni ,&nbsp;Gianni Zoccatelli ,&nbsp;Marco Ciulu ,&nbsp;Diana Vanessa Santisteban Soto ,&nbsp;Tommaso Sanson ,&nbsp;Tiziana Pandolfini ,&nbsp;Barbara Molesini","doi":"10.1016/j.stress.2025.100794","DOIUrl":"10.1016/j.stress.2025.100794","url":null,"abstract":"<div><div>Climate-driven increases in drought occurrence and the contamination of soils by heavy metals adversely affect tomato production both qualitatively and quantitatively. The aim of the present study was to investigate the responses of four inbred lines of <em>Solanum lycopersicum</em> to Ni toxicity and water deprivation. To evaluate the effects of Ni, we used two different growth approaches, in hydroponics and in soil, associating a morphological analysis of the lines with Ni and mineral nutrient quantification in both shoots and fruits. The effects of water stress were tested on germination capacity <em>in vitro</em> and on adult plants grown in soil. The responses of the different lines to water stress were assessed by physiological and phenotypic analyses, expression of drought-related genes and quantification of ABA. The multi-level approach allowed us to identify two lines, among the four investigated, as good candidates for future breeding programs due to their ability to accumulate less Ni and maintain fruit quality parameters and capacity to acclimate to repeated water stress.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100794"},"PeriodicalIF":6.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Rice sucrose non-fermenting related protein kinase (SnRK1) has a limited role in defense against Fall armyworm (Spodoptera frugiperda)” [Plant Stress, Volume 14, December 2024, 100667]","authors":"Devi Balakrishnan ,&nbsp;Vibha Srivastava ,&nbsp;Rupesh Kariyat","doi":"10.1016/j.stress.2025.100758","DOIUrl":"10.1016/j.stress.2025.100758","url":null,"abstract":"","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100758"},"PeriodicalIF":6.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143632172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated analysis of metabolomics and transcriptomics reveals the metabolites responsible for the antioxidant activity of water caltrop hull","authors":"Mingya Fang ,&nbsp;Guanhua Liu ,&nbsp;Wei Sheng ,&nbsp;Xiaoyang Chen ,&nbsp;Lin Shi ,&nbsp;Xiangtan Yao ,&nbsp;Lingyun Wang ,&nbsp;Zhaisheng Zheng","doi":"10.1016/j.stress.2025.100746","DOIUrl":"10.1016/j.stress.2025.100746","url":null,"abstract":"<div><div>The water caltrop (<em>Trapa</em> genus, Lythraceae family) fruit hulls are used as traditional herb medicines in China, Korea, and India due to their secondary metabolites. Although the hulls of multiple water caltrop varieties are applied as herb in these countries, few studies have assessed the metabolite diversity across water caltrop varieties. Here, six representative water caltrop varieties which were distributed in China and significant phenotypic difference, were chosen as the model to analysis the metabolome variation. The wild water caltrop and cultivated water caltrop have significant differences in size, volume, and weight of the fruit. The hulls of water caltrop had abundant secondary metabolites, including flavonoids, polyphenols, polysaccharides, and terpenoids. The metabolomes of the cultivated water caltrops were quite different from those of the wild ones. The abundance of flavonoids in the cultivated water caltrop hulls was lower than that in the wild hulls based on the UPL-MS analysis. The flavonoid composition also differed significantly among the water caltrop varieties. For instance, kaempferol-4′-<em>O</em>-glucoside was absent in three wild water caltrop varieties (Sijiaoyeling, Sliuling, and Geling). The antioxidant activities of the hull extracts of the cultivated water caltrop, SHL and WL, were similar. On the contrary, the extract of the wild water caltrops showed variation in both DPPH radical-scavenging and ferric reducing/antioxidant power. Subsequent analysis indicated that the flavonoids abundance was strongly correlated with the antioxidant activity. Besides, variations were detected in the expression levels of genes associated with flavonoid biosynthesis among the six water caltrop hulls. Comprehensively analysis of the metabolome and antioxidant activity of wild and cultivated species of water caltrop provide a new perspective for utilizing water caltrop varieties.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"16 ","pages":"Article 100746"},"PeriodicalIF":6.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal and plant-growth promoting potency of Streptomyces rochei against biotic stress caused by Race 4 Fusarium wilt on banana","authors":"Periakaruppan Jegan ,&nbsp;Saraswathy Sethurathinam ,&nbsp;Muthuvel Iyyamperumal ,&nbsp;Rajangam Jacob ,&nbsp;Angappan Kathithachalam ,&nbsp;Jayakanthan Mannu ,&nbsp;Soman Padmanabhan ,&nbsp;Manimaran Gajendiran","doi":"10.1016/j.stress.2025.100779","DOIUrl":"10.1016/j.stress.2025.100779","url":null,"abstract":"<div><div>Banana is a staple food for millions of individuals, especially in regions with tropical and subtropical climates. Nevertheless, the cultivation of banana is under significant threat from <em>Fusarium</em> wilt, a harmful biotic stress transmitted through the soil and caused by <em>Fusarium oxysporum</em> f. sp. <em>cubense</em> (<em>Foc</em>). This disease has the potential to devastate and infect almost all the varieties of bananas, especially with the emergence of <em>Foc</em> race 4. This study investigates the possibility of <em>Streptomyces rochei</em> AMBEAROOT2, isolated from the banana, used as a biocontrol agent against <em>Fusarium</em> wilt in banana. The molecular characterization of ten <em>Foc</em> isolates identified <em>Fusarium oxysporum</em> f. sp. <em>cubense</em> TFOC6 as the most virulent and confirmed as race 4. <em>S. rochei</em> AMBEAROOT2 exhibited significant mycelial inhibition (62.3%) of <em>F. oxysporum</em> f. sp. <em>cubense</em> TFOC6 and plant growth-promoting characteristics, including indole-3-acetic acid, biofilm formation, and exopolysaccharide synthesis. Gas Chromatography-Mass Spectroscopy analysis identified metabolites produced by <em>S. rochei</em> AMBEAROOT2, with molecular docking investigations revealed strong binding affinities of compounds <em>viz.,</em> 3-phenyl-3-p-tolyl-1-(2,4,6-trimethyl-phenyl)-propane-1-one, 19-acetoxy-4,4-dimethyl-, oxime, Androst-5-en-3-one, and Pyrano [4,3] benzopyran-1,9-dione to key virulence proteins (Catalase-peroxidase, Kynureninase, Penta functional AROM polypeptide, and Ribose phosphate pyrophosphokinase) of <em>Foc</em>. In greenhouse conditions, micro-propagated banana plantlets treated with <em>S. rochei</em> AMBEAROOT2 demonstrated enhanced growth, improved physiological traits, and higher levels of defense enzymes compared to those inoculated with <em>F. oxysporum</em> f. sp. <em>cubense</em> TFOC6 alone. It also induced systemic resistance and a 100% decrease in the incidence of <em>Fusarium</em> wilt. In conclusion, <em>S. rochei</em> AMBEAROOT2 showed potential antifungal activities, promoted plant growth, and could be used to manage wilt disease in banana after field evaluation.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100779"},"PeriodicalIF":6.8,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing macroalgal cell walls to trigger immunity in Arabidopsis thaliana","authors":"Jorge Peláez ,&nbsp;Carlos Frey ,&nbsp;Diego Rebaque ,&nbsp;Francisco Vilaplana ,&nbsp;Antonio Encina ,&nbsp;Hugo Mélida","doi":"10.1016/j.stress.2025.100783","DOIUrl":"10.1016/j.stress.2025.100783","url":null,"abstract":"<div><div>There is an increasing need to find sustainable alternatives to conventional agrochemicals to reduce biotic stress in crops. One possible strategy is based on promoting the innate defences of plants by stimulating their immune system. The plant immune system relies on the perception of molecules, which trigger a cascade of biochemical responses known as pattern-triggered immunity (PTI). This study investigated the potential of marine macroalgal cell wall components to be perceived by plants, act as elicitors of plant immune responses and induce disease resistance.</div><div>Cell walls of green, red, and brown algae species were chemically fractionated, and the research focused on testing their ability to induce immune responses in <em>Arabidopsis thaliana</em>. Different PTI hallmarks were tested, including H₂O₂ production, mitogen-activated protein kinases (MAPKs) phosphorylation, and defence gene expression analysis. The results showed that the CaCl₂-extracted fraction was particularly efficacious in inducing H₂O₂ production. As the CaCl₂ fraction of all phylogenetic groups also triggered additional immune responses, its ability to protect Arabidopsis against the bacterial pathogen <em>Pseudomonas syringae</em> was evaluated, confirming that certain CaCl₂ fractions successfully provided resistance to the pathogen. The monosaccharide and glycosidic linkage analysis of these fractions pointed to some specific algal cell wall glycans (e.g. porphyrans and fucoidans) that could contribute to the immunostimulatory capacity, thereby paving the way for the identification of distinct structures with potential agrobiological applications.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100783"},"PeriodicalIF":6.8,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143511675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing lodging resistance in maize: Integrating genetic, hormonal, and agronomic insights for sustainable crop productivity","authors":"Shumila Ishfaq ,&nbsp;Yi Ding ,&nbsp;Xiaoyan Liang ,&nbsp;Wei Guo","doi":"10.1016/j.stress.2025.100777","DOIUrl":"10.1016/j.stress.2025.100777","url":null,"abstract":"<div><div>Crop lodging, characterized by the bending or breaking of plant stems, poses a significant challenge to global food security by reducing crop yields and complicating harvesting processes. This review explores the factors influencing lodging susceptibility, including environmental conditions, genetic traits, fertilizer management, pathogens, and hormonal regulation. Recent advancements in maize research have uncovered critical genetic traits and elucidated the roles of key hormonal pathways—such as gibberellin (GA), strigolactone (SL), auxin, and ethylene—in modulating stem elongation, tillering angles, and root system architecture. These pathways collectively shape crop architecture, with GA and SL contributing to stalk strength, and auxin and ethylene enhancing root development and plant stability. Concurrently, agronomic interventions, such as optimized planting density and nutrient management, have improved stem integrity and mitigated lodging risk. By integrating genetic, hormonal, and agronomic knowledge, researchers have made remarkable progress in developing maize varieties that resist lodging, enhancing crop resilience and yield stability under various environmental conditions. Future research should focus on unraveling the molecular and genetic mechanisms underlying lodging resistance, addressing technical limitations in implementation, and advancing sustainable agricultural practices to secure global food production and ensure long-term productivity.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100777"},"PeriodicalIF":6.8,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143463334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNA-Seq-based analysis of transcriptomic signatures elicited by mutations conferring salt tolerance in Cucurbita pepo","authors":"Keshav Gautam,&nbsp;Sonsoles Alonso,&nbsp;Alicia García,&nbsp;María Segura,&nbsp;Álvaro Benítez,&nbsp;Cecilia Martínez,&nbsp;Manuel Jamilena","doi":"10.1016/j.stress.2025.100775","DOIUrl":"10.1016/j.stress.2025.100775","url":null,"abstract":"<div><div>Salinity is a major determinant of plant growth and crop productivity, resulting in significant economic losses in agriculture. Improving salinity tolerance in plant breeding programs requires not only donor tolerant genotypes but also a thorough knowledge of the genes controlling the trait. Taking advantage of two recently identified salinity-tolerant EMS mutants of squash (<em>sal-1</em> and <em>sal-2</em>), this study aimed to analyse whether these two sources of salt tolerance are associated with similar transcriptomic changes in leaves. RNA sequencing revealed that the two mutants have a very distinct transcriptomic response to salt stress compared to the WT, with 154 and 1068 salt-tolerance-associated differentially expressed genes (DEGs) in <em>sal-1</em> and <em>sal-2</em>, respectively. GO and KEGG enrichment analyses revealed the importance of several phytohormone biosynthesis, signalling and transport genes (<em>CpAUX22B/22D, CpSAUR32–2, CpARR5/12, CpAHK2/3, CpBZR1, CpTCH4, CpNCED1, CpCYP707A1, CpPP2C, CpSnRK1/2, CpLOX2</em> and <em>CpACX</em>) in the salt tolerance response. MAPK genes (<em>CpMPK3</em> and <em>CpMEKK1</em>) and the Ca²⁺ signalling network (<em>CpCPK26/28/34, CpCML31/36/48, CpPBP1, CpCBL1</em> and <em>CpRBOHD</em>) were also specifically activated in salt-tolerant mutants, indicating their contribution to salt tolerance. Genes for antioxidant enzymes (PP2, POD, CAT, PRX, GST and GRX) and cell wall metabolism were also up-regulated in salt-tolerant mutants, reducing oxidative stress and maintaining the integrity of membranes and other cellular structures. Genes for ion transporters were significantly up-regulated in response to salt stress in <em>sal-2</em>, probably involved in maintaining ion homeostasis. Several genes encoding transcription factors of the ERF, C3H, Dof, HD-ZIP, MYB, HSF, NAC, knotted and WRKY families, as well as long non-coding RNA, were also found to positively or negatively regulate salt stress tolerance in the <em>sal-1</em> and <em>sal-2</em> mutants. Overall, the results highlight the complexity of the molecular response involved in salt stress tolerance in <em>C. pepo</em> and prioritise further investigation of specific genes that contribute to the resilience of crops under saline conditions.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100775"},"PeriodicalIF":6.8,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}