Synergistic effects of ectoine and biostimulants combinations on tomato seedling growth and heat stress resilience

IF 6.8 Q1 PLANT SCIENCES

Plant Stress Pub Date : 2025-04-27 DOI:10.1016/j.stress.2025.100873

Yanyan Chen , Weimin Xu , Chuankun Han , Miao Zhang , Ying Chen , Yujia Ma , Xinjun Zhang , Huhu Liu , Wenna Zhang

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Abstract

Biostimulants play a crucial role in promoting plant growth and stress tolerance. In this study, we investigated the effects of single and combined applications of ectoine (E), myo-inositol (MI), corn steep liquor (CSL), and hydrogen-rich water (HRW) on the growth and heat tolerance of tomato seedlings. Our results demonstrated that the combined application of these biostimulants significantly enhanced plant growth and physiological characteristics. Among the treatments, HCE (HRW + CSL + E) exhibited the most pronounced effects on root growth, increasing root length, volume, and surface area, while E and HE (HRW + E) demonstrated the highest phenotypic index and stress resilience under heat stress conditions. Transcriptomic analysis revealed significant differences in differentially expressed genes (DEGs) among treatments. The HCE treatment exhibited the highest number of DEGs, particularly in pathways related to photosynthesis, carbon fixation, plant hormone signal transduction. Functional enrichment analysis showed that H and E treatments were primarily involved in plant-pathogen interactions and hormone signaling, whereas HE and HCE treatments were associated with carbon and energy metabolism. The combined application of biostimulants demonstrated synergistic effects, enhancing multiple physiological pathways that contribute to plant heat stress adaptation. These findings provide valuable insights of the molecular mechanisms underlying biostimulant-induced growth promotion and stress resilience in tomato seedlings. The results highlight the potential of biostimulant combinations for optimizing crop production in controlled environments, offering a promising strategy for sustainable and high-efficiency agriculture.

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异托碱和生物刺激剂组合对番茄幼苗生长和热胁迫抗逆性的协同效应

生物刺激素在促进植物生长和抗逆性方面起着至关重要的作用。本试验研究了异托因(E)、肌醇（MI）、玉米浸泡液（CSL）和富氢水（HRW）单独和联合施用对番茄幼苗生长和耐热性的影响。我们的研究结果表明，这些生物刺激素的联合应用显著提高了植物的生长和生理特性。在热胁迫条件下，HCE （HRW + CSL + E）对根生长、根长、根体积和根表面积的影响最为显著，而E和HE （HRW + E）表现出最高的表型指数和抗逆性。转录组学分析显示，不同处理间差异表达基因（DEGs）存在显著差异。HCE处理的deg数量最多，特别是在光合作用、碳固定和植物激素信号转导相关的途径中。功能富集分析表明，H和E处理主要参与植物与病原体的相互作用和激素信号传导，而HE和HCE处理则与碳和能量代谢有关。生物刺激素的联合应用显示出协同效应，增强了植物适应热胁迫的多种生理途径。这些发现为揭示生物刺激素诱导的番茄幼苗生长促进和抗逆性的分子机制提供了有价值的见解。研究结果强调了生物刺激素组合在受控环境下优化作物生产的潜力，为可持续和高效农业提供了一种有前途的策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Stress PLANT SCIENCES-

CiteScore

5.20

自引率

8.00%

发文量

审稿时长

63 days

期刊介绍： The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.