TaPUB57 confers drought tolerance, governs grain size and salt sensitivity by ubiquitinating TaEXPB3 in rice

IF 6.8 Q1 PLANT SCIENCES

Plant Stress Pub Date : 2025-05-03 DOI:10.1016/j.stress.2025.100877

Chunmei Yu , Min Wang , Long Li , Yuying Li , Yanfei Zhang , Jie Zhang , Xin Chen , Lili Yang , Chaonan Li , Jingyi Wang , Guangchao Liu , Matthew P Reynolds , Qi Xie , Ruilian Jing , Xinguo Mao

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引用次数: 0

Abstract

Wheat (Triticum aestivum L.) is the widest cultivated crop in the world. Abiotic stress, such as drought and high salinity, dramatically impacts the growth and development of wheat and leads to remarkable yield loss. Understanding the underlying mechanisms of abiotic stress tolerance is of great importance to develop high yield varieties with wide adaptability. Ubiquitination is a major type of post-translational modification in eukaryotes. The plant U-Box (PUB) protein is the smallest family in the E3 ligase superfamily, and involved in the responses to various environmental stimuli. Currently, TaPUB57 has been cloned from wheat. It was induced by multiple abiotic stresses and phytohormone. Its ectopic expression increased grain size and drought tolerance, but caused hypersensitive to salt stress in rice. TaPUB57 interacted with and ubiquitinated TaEXPB3. Constitutive expression of TaEXPB3 resulted in small grain size and remarkably enhanced salt tolerance. Moreover, TaPUB57/TaEXPB3 co-expressing rice plants exhibited phenotypes of salt sensitivity and larger grain size relative to TaEXPB3 transgenic lines. Therefore, it is speculated that TaPUB57 acts on grain size and the salt tolerance by ubiquitinating TaEXPB3.

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taub57通过泛素化TaEXPB3调控水稻的抗旱性、晶粒大小和盐敏感性

小麦（Triticum aestivum L.）是世界上种植最广的作物。干旱、高盐等非生物胁迫严重影响小麦的生长发育，造成显著的产量损失。了解非生物抗逆性的潜在机制对培育具有广泛适应性的高产品种具有重要意义。泛素化是真核生物翻译后修饰的主要类型。植物U-Box （PUB）蛋白是E3连接酶超家族中最小的家族，参与对各种环境刺激的反应。目前，TaPUB57已经从小麦中克隆出来。它是由多种非生物胁迫和植物激素诱导的。其异位表达增加了水稻的晶粒尺寸和耐旱性，但引起水稻对盐胁迫的超敏反应。TaPUB57与TaEXPB3相互作用并泛素化。TaEXPB3的组成性表达使玉米籽粒变小，耐盐性显著增强。此外，与TaEXPB3转基因株系相比，共表达TaPUB57/TaEXPB3的水稻表现出盐敏感表型和更大的晶粒尺寸。因此，推测TaPUB57通过泛素化TaEXPB3影响晶粒尺寸和耐盐性。

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来源期刊

Plant Stress PLANT SCIENCES-

CiteScore

5.20

自引率

8.00%

发文量

审稿时长

63 days

期刊介绍： The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.