NIN-like proteins in poplar play roles in responding to abiotic stresses and nitrate availability

IF 6.8 Q1 PLANT SCIENCES

Plant Stress Pub Date : 2025-05-04 DOI:10.1016/j.stress.2025.100878

Lianke Guan , Yan Lu , Hang Wang , Zihui Li , Qi Li , Jie Luo

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引用次数: 0

Abstract

NIN-like proteins (NLPs) play crucial roles in NO₃^- signaling pathway, however, knowledge of NLPs in woody plants remains limited. A total of 13 NLP genes were identified in each haplotype of 717 poplar. All NLPs contained the PB1 and RWP-RK domains. Eight and nine duplicated gene pairs were detected for PtNLPs and PaNLPs, respectively. Promoter analysis of NLP genes in 717 poplar revealed the existence of many cis-elements of light and stress responses. The NLP genes exhibited distinct expression profiles across different tissues. For instance, NLP1-NLP3 were nearly undetectable in leaves but highly accumulated in roots. The response of NLP genes to NO₃^- availability also showed complex patterns: NLP1, NLP3, NLP5, NLP10 and NLP11, were induced by high NO₃⁻ availability in roots, but NLP6, NLP12 and NLP13 genes were inhibited by high NO₃^- conditions in both roots and leaves. Additionally, a protein interaction network centered around poplar NLP proteins was constructed, highlighting their potential roles in carbon and nitrogen (N) metabolism. These findings underscore the potential roles for poplar NLP genes in NO₃^- signaling and stress adaptation and lay a foundation for stimulating N use efficiency (NUE) in woody plants.

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杨树nin样蛋白在响应非生物胁迫和硝态氮有效性中起重要作用

nlp在NO3-信号通路中发挥着重要作用，但对木本植物nlp的了解仍然有限。在717株杨树的每个单倍型中共鉴定出13个NLP基因。所有nlp均含有PB1和RWP-RK结构域。PtNLPs和PaNLPs分别检测到8对和9对重复基因。对717株杨树NLP基因的启动子分析表明，NLP基因中存在许多光逆境响应的顺式元件。NLP基因在不同组织中表现出不同的表达谱。例如，NLP1-NLP3在叶片中几乎检测不到，但在根中大量积累。NLP基因对NO3-可用性的反应也表现出复杂的模式：NLP1、NLP3、NLP5、NLP10和NLP11是由根的高NO₃⁻可用性诱导的，而NLP6、NLP12和NLP13基因是由根和叶的高NO3-可用性抑制的。此外，构建了以杨树NLP蛋白为中心的蛋白质相互作用网络，突出了它们在碳氮代谢中的潜在作用。这些发现提示了杨树NLP基因在NO3-信号转导和胁迫适应中的潜在作用，为提高木本植物氮素利用效率（NUE）奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Stress PLANT SCIENCES-

CiteScore

5.20

自引率

8.00%

发文量

审稿时长

63 days

期刊介绍： The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.