Transcriptome analysis revealed the molecular mechanism of the response to leaf spot disease in Camellia sasanqua

IF 6.8 Q1 PLANT SCIENCES

Plant Stress Pub Date : 2025-08-27 DOI:10.1016/j.stress.2025.101015

Hongye Zhang, Cheng Yang, Lei Zong, Longqing Chen, Tian Wu

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Abstract

The pathogens on the leaves of the Camellia sasanqua seriously affected the ornamental value. Illuminating the underlying molecular mechanisms is of extraordinary importance in C. sasanqua to improve resistance variety. We isolated the pathogens of Fusarium sambucinum, Alternaria alternata, Phyllosticta capitalensis, and Diaporthe amygdali from the diseased leaves of C. sasanqua. These four pathogens might cause the leaf spot of C. sasanqua by working together. The transcriptomic analysis detected differences between healthy and diseased leaf samples of C. sasanqua, and 8139 DEGs were identified, including 4544 up-regulated genes and 3595 down-regulated genes. KEGG enrichment analysis of the canonical defensive pathways and the genes with high expression levels indicated that the response of C. sasanqua to pathogens was a complex signal network, including signal recognition and transmission, plant hormones including SA and ABA, activation of transcription factors of MYB, AP2/ERF, WRKY, and secondary metabolic accumulation. The genes of the phenylpropanoid biosynthesis pathway were expressed significantly in the response processes. We further cloned the genes of CAD (Cinnamic alcohol dehydrogenase) and COMT (Caffeic acid 3-O-methyltransferase), respectively, in the phenylpropanoid biosynthesis pathway, and then characterized their functions in tobacco. The results showed that overexpressing CsCAD5 and CsCOMT1 could influence the accumulation of lignins. We speculated that the increased lignin content in plants might be achieved by thickening and lignifying the cell wall. In summary, our findings discussed the complexities and interactions of C. sasanqua responses to leaf spot, identifying potential resistance genes and molecular mechanisms for preventing and controlling plant diseases.

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转录组分析揭示了山茶对叶斑病反应的分子机制

山茶叶片病原菌严重影响其观赏价值。阐明其潜在的分子机制，对提高柽柳抗病品种具有重要意义。从三瓜病叶中分离出三灰镰刀菌、互花镰刀菌、毛条镰刀菌和桃核镰刀菌。这四种病原菌可能通过共同作用而引起沙三瓜叶斑病。转录组学分析结果显示，三瓜健康和患病叶片样本存在差异，共鉴定出8139个基因，其中上调基因4544个，下调基因3595个。对典型防御途径和高表达基因的KEGG富集分析表明，沙三瓜对病原菌的响应是一个复杂的信号网络，包括信号识别和传递、SA和ABA等植物激素、MYB、AP2/ERF、WRKY等转录因子的激活以及次生代谢积累。在响应过程中，苯丙素生物合成途径相关基因显著表达。我们进一步克隆了苯丙素生物合成途径中的肉桂醇脱氢酶（Cinnamic alcohol dehydrogenase）和咖啡酸3- o -甲基转移酶（COMT）基因，并对其在烟草中的功能进行了表征。结果表明，过表达CsCAD5和CsCOMT1可以影响木质素的积累。我们推测植物中木质素含量的增加可能是通过细胞壁增厚和木质化来实现的。综上所述，我们的研究结果讨论了沙三瓜对叶斑病反应的复杂性和相互作用，鉴定了潜在的抗性基因和预防和控制植物病害的分子机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Stress PLANT SCIENCES-

CiteScore

5.20

自引率

8.00%

发文量

审稿时长

63 days

期刊介绍： The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.