VIEWPub Date : 2024-02-01DOI: 10.1002/viw.20230086
Jie Luo, Binpan Wang, Xiaoqi Tang, Ping Huang, Sha Yang, Shuang Zhao, Shuang Xie, Qiaofeng Li, Kai Chang, Ming Chen
{"title":"CRISPR/Cas12a-loaded intelligent DNA hydrogel for universal and ultrasensitive exosome assay","authors":"Jie Luo, Binpan Wang, Xiaoqi Tang, Ping Huang, Sha Yang, Shuang Zhao, Shuang Xie, Qiaofeng Li, Kai Chang, Ming Chen","doi":"10.1002/viw.20230086","DOIUrl":"https://doi.org/10.1002/viw.20230086","url":null,"abstract":"Tumor-derived exosomes are crucial for early non-invasive and accurate tumor diagnosis in clinical diagnostics. The development of highly sensitive, simple, and intuitive exosome assays has sparked a research upsurge in clinical diagnostics. Here, we develop a bio-responsive intelligent DNA hydrogel loaded with CRISPR/Cas12a for universal and ultrasensitive detection of the exosomes. The aptamer serves as the target response unit and switch, competitively disintegrating the region of the DNA linkers and then Cas12a/crRNA was released and activated, resulting in a high fluorescent intensity for exosome detection at the detection limit of 119 particles/μL. Moreover, a constructed colorimetric tube is made by loading a colorimetric filter membrane on the tube lid and intelligent DNA hydrogel on the tube bottom, which enables one-pot portable colorimetric detection. Without the need for laboratory instruments and professionals, this strategy allows for naked eye detection with limit of detection as low as 10<sup>4</sup> particles/μL, and shows great applicability in distinguishing between healthy individuals, pretreatment patients, and post-treatment patients after obtaining a testable analyte. In this study, an ultrasensitive detection platform for exosomes that enables one-step sensing and dual signal output was introduced. The findings here suggest that this platform is a promising tool for the application of liquid biopsy based on exosomes in clinical diagnosis.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139677490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
VIEWPub Date : 2024-01-15DOI: 10.1002/viw.20230046
Hongcheng Mai, Dan Lu
{"title":"Tissue clearing and its applications in human tissues: A review","authors":"Hongcheng Mai, Dan Lu","doi":"10.1002/viw.20230046","DOIUrl":"https://doi.org/10.1002/viw.20230046","url":null,"abstract":"In three-dimensional (3D) space, an unbiased and systemic view of human specimens between structures and functions is required. However, conventional histological sections from specimens have made only limited progress in exploring intact information about 3D biological tissues. With significant advances in optical physics and chemical engineering, state-of-the-art tissue-clearing methods can revolutionize the intact subcellular level of human tissue histological analysis, from thick human tissues to intact human organs. The present review summarizes the principle of tissue clearing so that a trainee researcher can implement effective human tissue-clearing protocols. Furthermore, this review highlights existing tissue-clearing methods in specific human tissue applications, describes imaging strategies, and presents various efficient computational approaches for processing and visualizing large image data. Finally, potential future directions for developing tissue-clearing methods for human tissues are also discussed.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139469117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Super-resolution ultrasound localization microscopy for the non-invasive imaging of human testicular microcirculation and its differential diagnosis role in male infertility","authors":"Maoyao Li, Lei Chen, Jipeng Yan, Channa Nalin Jayasena, Zhangshun Liu, Jia Li, Ao Li, Jiang Zhu, Ronghui Wang, Jianchun Li, Chaoxue Zhang, Jingyi Guo, Yuwu Zhao, Chao Feng, Mengxing Tang, Yuanyi Zheng","doi":"10.1002/viw.20230093","DOIUrl":"https://doi.org/10.1002/viw.20230093","url":null,"abstract":"Testicular microcirculation is closely related to spermatogenic function and seminiferous tubular function. The diagnosis and monitoring of testicular diseases can be associated with testicular microcirculation; however, there are currently no effective non-invasive methods for super-resolution imaging of testicular microcirculation. In this study, we introduced state-of-the-art graph-based tracking with the Kalman motion model algorithm to non-invasively image human testicular microcirculation for the first time with a regular frame-rate clinical ultrasound imaging system (37 Hz). Two distinct testicular vessels with an 81 µm separation were resolved in the testicular vasculature, surpassing all other imaging modalities. In a retrospective study, we performed contrast-enhanced ultrasound examinations(CEUS) and ultrasound localization microscopy (ULM) processing on the included 76 infertile patients and 15 healthy controls from August 2021 to May 2023 and obtained super-resolution images of testicular microcirculation with sub-diffraction resolution. Through the results of one-way analysis of variance tests and receiver operating characteristic analyses, we found that the ULM-based parameters hold promise as clinical guidance for differentiating between non-obstructive and obstructive male infertility. The mean vessel diameter achieved an area under the curve (AUC) of 0.920 (95% confidence interval [CI]: 0.847–0.994, <i>p</i> < .001) with a cut-off value of 170.9 µm in oligoasthenospermia, and an AUC of 0.952 (95% CI: 0.875–1.000, <i>p</i> < .001) with a cut-off value of 169.9 µm in azoospermia patients, respectively, addressing a significant clinical challenge.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139461633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Three-dimensional printed biomimetic multilayer scaffolds coordinated with sleep-related small extracellular vesicles: A strategy for extracellular matrix homeostasis and macrophage polarization to enhance osteochondral regeneration","authors":"Xu-Ran Li, Qing-Song Deng, Po-Lin Liu, Shu-Hang He, Yuan Gao, Zhan-Ying Wei, Chang-Ru Zhang, Fei Wang, Xiao-Qiu Dou, Helen Dawes, Shang-Chun Guo, Shi-Cong Tao","doi":"10.1002/viw.20230069","DOIUrl":"https://doi.org/10.1002/viw.20230069","url":null,"abstract":"Cartilage defects resulting from injury or degeneration are a common clinical problem, and due to its avascular nature, articular cartilage has poor self-healing capacity. Three-dimensional (3D) bioprinting has attracted great attention in tissue engineering. Melatonin (MT), a hormone mainly secreted at night, plays an important role in tissue repair. Small extracellular vesicles (sEV) are considered ideal drug delivery vehicles and MT-sEV (sleep-related sEV) have the potential ability to promote cartilage regeneration. Here, biomimetic multilayer scaffolds were fabricated using 3D bioprinting. A double network hydrogel, composed of methacrylated hyaluronic acid and gelatin methacryloyl (HG), was prepared. MT-sEV and HG hydrogel were used to create a cartilage layer. A bone layer was formed using poly(ε-caprolactone) and hydroxyapatite ultralong nanowires. Additionally, two bioinks were alternately printed at the interface layer. The results of RNA sequencing revealed the potential regulatory mechanisms. MT-sEV showed promotional effects on cell migration, proliferation, chondrogenic differentiation, and extracellular matrix (ECM) deposition. Moreover, MT-sEV altered macrophage polarization and regulated the expression of inflammatory cytokines. In vivo experiments demonstrated that the biomimetic multilayer scaffolds promoted cartilage regeneration. These experiments demonstrated the ability of MT-sEV to regulate the immune microenvironment and promote the secretion of ECM, providing a promising strategy for cartilage regeneration.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139422465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
VIEWPub Date : 2023-12-29DOI: 10.1002/viw.20230100
Yuanyuan Wu, Pingping Wang, Xinyu Zhao, Ti Liu, Bo Situ, Lei Zheng
{"title":"Dynamic interplay of intratumoral microbiota within the colorectal cancer microenvironment","authors":"Yuanyuan Wu, Pingping Wang, Xinyu Zhao, Ti Liu, Bo Situ, Lei Zheng","doi":"10.1002/viw.20230100","DOIUrl":"https://doi.org/10.1002/viw.20230100","url":null,"abstract":"Colorectal cancer (CRC) is a complex malignancy, influenced not only by cancer cells but also by the tumor microenvironment (TME). Within the TME, emerging evidence highlights the presence and functional roles of diverse microbial entities, referred to as intratumoral microbiota. The distribution of these microbiota exhibits significant heterogeneity and engages in dynamic interactions with tumor cells, forming a unique ecosystem. Certain bacterial strains distinctly influence the TME of CRC, affecting the characteristics and progression of the tumor. This review summarizes the intricate roles of intratumoral microbiota within CRC's TME, emphasizing their importance in the disease's development and progression, and discuss the opportunities and challenges in the field.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139068044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Inside Front Cover: Portable non-enzymatic electrochemical biosensor based on caffeine for Alzheimer's disease diagnosis (View 6/2023)","authors":"Xindan Zhang, Audrey Wang, Chaojie Wang, Xiaoying Tang, Zhenqi Jiang, Jieling Qin","doi":"10.1002/viw2.328","DOIUrl":"https://doi.org/10.1002/viw2.328","url":null,"abstract":"In article number 20230052, Jieling Qin, Zhenqi Jiang and their co-workers have described caffeine as a biorecognition element to detect amyloid beta 1-42 (Aβ1-42), a biomarker of Alzheimer's disease. In this cover, caffeine was bound to a gold electrode modified with pine polythiophene-3-acetic acid and recognized Aβ1-42 by intermolecular forces. This strategy demonstrates good detection performance in real sample analysis and enables the development of portable instruments to achieve rapid detection of Aβ1-42, suggesting the potential for practical applications.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139068364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
VIEWPub Date : 2023-12-20DOI: 10.1002/viw.20230053
Miaomiao Fei, Hui Zhang, Fanbing Meng, Guanghui An, Jinxuan Tang, Jianbin Tong, Lize Xiong, Qidong Liu, Cheng Li
{"title":"Enhanced lactate accumulation upregulates PD-L1 expression to delay neutrophil apoptosis in sepsis","authors":"Miaomiao Fei, Hui Zhang, Fanbing Meng, Guanghui An, Jinxuan Tang, Jianbin Tong, Lize Xiong, Qidong Liu, Cheng Li","doi":"10.1002/viw.20230053","DOIUrl":"https://doi.org/10.1002/viw.20230053","url":null,"abstract":"Malfunction of neutrophil apoptosis and elevated serum lactate levels are the key cellular mechanism of immune suppressive status in septic patients. However, whether increased lactate affects apoptosis of neutrophils and aggravates sepsis development, and the molecular mechanism remain unknown. In this study, first, we analyzed the transcriptional profiles of blood cells in sepsis patients (n = 39) and healthy volunteers (n = 40) to reveal that there is close correlation between the lactate-related gene expression changes associated with lactate production and immune function in leukocytes, especially in neutrophils. Further, we explored the close relationship between lactate and delayed neutrophil apoptosis in human neutrophils. Programmed cell death 1 legand (PD-L1) was highly expressed in septic patients compared with healthy volunteers. Then, we indicated that elevated levels of lactate in human neutrophils decreased neutrophil apoptosis (<i>P</i> < .001) by upregulating PD-L1 expression. Inhibition of monocarboxylate transporter 1 (MCT1) significantly attenuated neutrophil apoptosis caused by lactate (<i>P</i> < .001). We further performed in vivo experiments in sepsis mice model and determined that increased lactate decreased neutrophil apoptosis (<i>P</i> < .05) and reduces mice survival rate (<i>P</i> < .001), which could also be rescued by MCT1 inhibitor (<i>P</i> < .05). This study revealed that elevated level of lactate in sepsis upregulates PD-L1 expression to decrease apoptosis through MCT1 in neutrophils, which provides new insight into sepsis treatment strategy by reducing lactate accumulation.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138823408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
VIEWPub Date : 2023-12-17DOI: 10.1002/viw.20230070
Lei Xu, Yujiao Xie, Jie Lin, Aiguo Wu, Tianan Jiang
{"title":"Advancements in SERS-based biological detection and its application and perspectives in pancreatic cancer","authors":"Lei Xu, Yujiao Xie, Jie Lin, Aiguo Wu, Tianan Jiang","doi":"10.1002/viw.20230070","DOIUrl":"https://doi.org/10.1002/viw.20230070","url":null,"abstract":"Surface-enhanced Raman spectroscopy (SERS) has become an essential bio-detection technique. Due to its high sensitivity, good signal specificity, and resistance to photobleaching, SERS has been widely used in biomedical research fields such as molecular imaging, tumor diagnosis, and drug monitoring. This review focuses on the progress of SERS in biomedical applications. We first introduce the basic principle of SERS and the progress of substrate research. Then, we summarize the latest research progress on SERS in drug monitoring, cell and exosome detection, tumor imaging, and detection platforms combining microfluidic and lateral flow technologies. Subsequently, the applied research of SERS in early diagnosis of pancreatic cancer and drug efficacy monitoring is described. Finally, the future development direction and possible challenges of SERS in tumor diagnosis and treatment are proposed.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2023-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138680380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The PES1/FOXM1 heterodimer suppresses TCF21 and ERβ expression in ovarian endometriosis","authors":"Jingwen Zhu, Peili Wu, Ruihui Lu, Cheng Zeng, Chao Peng, Yingfang Zhou, Qing Xue","doi":"10.1002/viw.20230090","DOIUrl":"https://doi.org/10.1002/viw.20230090","url":null,"abstract":"Transcription factor 21 (TCF21) and estrogen receptor beta (ERβ, encoded by ESR2) are highly expressed in endometriotic stromal cells (ESCs) and contribute to the pathogenesis of endometriosis. However, the exploration of TCF21 and ERβ expression regulation at the molecular level remains limited. Here, by using bioinformatics analysis and experimental verification, we identified PES1, also known as Pescadillo, as a negative regulator in the development of endometriosis that downregulates TCF21 and ERβ expression in ESCs. PES1 overexpression regulated critical biological processes involved in endometriosis development, such as invasion and apoptosis. A coimmunoprecipitation assay showed that PES1 could form a complex with Forkhead box M1 (FOXM1). Further analyses elucidated that siPES1 in ectopic lesions decreased the stability of FOXM1 protein and reduced the binding activities of FOXM1 to TCF21 and ESR2 promoters, thus weakening the transcriptional inhibition of TCF21 and ERβ by FOXM1. Moreover, in an endometriosis mouse model, overexpressing PES1 effectively reduced the growth of ectopic lesions and suppressed TCF21 and ERβ expression, which suggests a promising therapeutic strategy for endometriosis. Collectively, our results indicate that the loss of PES1 in ectopic lesions contributes to endometriosis progression by upregulating ERβ and TCF21 expression through heterodimer formation with FOXM1. Moreover, targeting PES1 could serve as a treatment method for endometriosis.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2023-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138572201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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