IF 8.6 4区医学

VIEW Pub Date : 2024-06-25 DOI: 10.1002/viw.20240017

Jing Wang, Qitao Song, Tao Yang, Yuanli Li, Lihua Zhang, Jiayan Li, Feifei Liu, Yanyin Lin, Xiaoxia Xu, Yu Heng, Lulai Xu, Shun Zhang, Jiahui Zhou, Yunbo Liu, Lingyuan Kong, Dingbin Tang, Chengdong Ji, Bing Tan, Pu Liao, Nengke Pan, Weijing Yi, Zhanhui Wang

{"title":"The challenges and breakthroughs in the development of diagnostic monoclonal antibodies","authors":"Jing Wang, Qitao Song, Tao Yang, Yuanli Li, Lihua Zhang, Jiayan Li, Feifei Liu, Yanyin Lin, Xiaoxia Xu, Yu Heng, Lulai Xu, Shun Zhang, Jiahui Zhou, Yunbo Liu, Lingyuan Kong, Dingbin Tang, Chengdong Ji, Bing Tan, Pu Liao, Nengke Pan, Weijing Yi, Zhanhui Wang","doi":"10.1002/viw.20240017","DOIUrl":"https://doi.org/10.1002/viw.20240017","url":null,"abstract":"Over the past century, the field of antibody discovery has undergone significant evolution, excluding the current exploration stage of artificial intelligence-based antibody generation and the often overlooked non-animal sourced antibody discovery, which typically requires mature in vitro affinity and the selection of high-quality antigen formulations. This journey has traversed various stages, from methods involving serum-based antibody acquisition, the isolation of B cells capable of perpetual antibody production through hybridoma technology, to the in-depth exploration of genetic material using the phage display system, and the current stage involving diverse single B cell screening techniques. Additionally, the emergence of machine learning has brought impressive scientific and technological breakthroughs across research domains, proving to be a powerful application in the field of antibody discovery. However, each technique comes with its limitations, such as variability and control challenges in serum-based acquisition, lengthy and difficult hybridoma-derived antibody development, potential limitations in sequence and epitope diversity due to immunization biases in phage display techniques, and costly single B cell screening. Protein mass spectrometry sequencing, with shorter acquisition time and lower costs, is seen as a shortcut by diagnostic companies, impacting traditional antibody development. In diagnostic antibody development, methodological differences in downstream assays and the impact of constant regions outside the Fv core are often neglected. This paper deeply analyzes challenges, proposing innovative strategies for the next generation of diagnostic antibody development. Aimed at moving closer to the gold standard of antibody discovery, these strategies enhance the competitiveness of diagnostic reagent products.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141510502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An intelligent biosensing platform using phase space reconstruction-assisted convolutional neural network for drug-induced cardiotoxicity assessment 利用相空间重构辅助卷积神经网络评估药物诱导的心脏毒性的智能生物传感平台

IF 8.6 4区医学

VIEW Pub Date : 2024-06-20 DOI: 10.1002/viw.20230096

Wenjian Yang, Xinyu Guo, Ruochen Wu, Yue Wu, Minzhi Fan, Bihu Lv, Diming Zhang, Zhijing Zhu

{"title":"An intelligent biosensing platform using phase space reconstruction-assisted convolutional neural network for drug-induced cardiotoxicity assessment","authors":"Wenjian Yang, Xinyu Guo, Ruochen Wu, Yue Wu, Minzhi Fan, Bihu Lv, Diming Zhang, Zhijing Zhu","doi":"10.1002/viw.20230096","DOIUrl":"https://doi.org/10.1002/viw.20230096","url":null,"abstract":"Drug-induced cardiotoxicity often leads to patient deaths and drug recalls. Interdigitated electrode (IDE)-based cellular impedance detection instrument has been integrated with intelligent algorithms to screen cardiotoxicity based on cardiomyocytes. These intelligent biosensing systems generally employ traditional machine learning methods to assess drug-induced cardiotoxicity by analyzing cardiomyocytes mechanical beating signals. However, modern deep learning methods with robustness and flexibility have not been integrated in IDE-based platform to screen cardiotoxicity. Here, for the first time, we implemented deep convolutional neural network (CNN) to analyze cardiomyocytes mechanical beating signals for cardiotoxicity assessment. This method can eliminate the feature engineering procedures, such as manual design and extraction of signal features required by traditional machine learning methods. To facilitate two-dimensional (2-D) CNN analysis, we utilized phase space reconstruction to convert one-dimensional beating signals into 2-D image representations as well as take into account nonlinear dynamic information. The phase space reconstruction-assisted convolutional neural network (PSRCNN) is capable of accurately categorizing drug-induced cardiotoxicities and predicting cardiotoxicity levels. It obtains accuracies ranging from 0.82 to 0.99 when recognizing the cardiotoxicity of newly developed drugs, which are represented by drugs that are not used during model training. Furthermore, we explore in depth to compare the performance of CNN with other traditional machine learning methods. The PSRCNN method can achieve higher accuracies when compared to other methods. The PSRCNN-based biosensing platform is highly potential in improving the efficiency and accuracy of high-throughput screening of newly developed drugs for cardiotoxicity during drug discovery.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141510503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pulmonary coinfection by Pneumocystis jirovecii and Aspergillus terreus in an ITP patient after corticosteroid therapy: A case report 一名接受皮质类固醇治疗的 ITP 患者合并感染肺孢子虫和土曲霉：病例报告

IF 8.6 4区医学

VIEW Pub Date : 2024-06-03 DOI: 10.1002/viw.20230051

Lili Wang, Fengling Wang, Enqiang Mao, Erzhen Chen, Dayu Chen, Linyu Wang, Yusi Qiu, Xiaolan Bian, Yan Li, Juan He

{"title":"Pulmonary coinfection by Pneumocystis jirovecii and Aspergillus terreus in an ITP patient after corticosteroid therapy: A case report","authors":"Lili Wang, Fengling Wang, Enqiang Mao, Erzhen Chen, Dayu Chen, Linyu Wang, Yusi Qiu, Xiaolan Bian, Yan Li, Juan He","doi":"10.1002/viw.20230051","DOIUrl":"https://doi.org/10.1002/viw.20230051","url":null,"abstract":"Pneumocystis jirovecii pneumonia and invasive pulmonary aspergillosis are both life-threatening opportunistic fungal infections. There are only few reports of coinfection by these two fungi in the literature, and Aspergillus fumigatus is the predominant Aspergillus species in the coinfection. We report here the first case of coinfection by Aspergillus terreus and P. jirovecii pneumonia and caspofungin can be an appropriate choice for salvage treatment of the coinfection. A 51-year-old man with a history of immune thrombocytopenia treated with prednisone over 2 months was admitted to emergency intensive care unit for acute respiratory failure and a cavity was found on chest computed tomography. Therefore, his trachea was immediately intubated. The patient was treated with a large spectrum of antibiotic regimen, consisting initially of imipenem/cilastatin, moxifloxacin and fluconazole followed by fluconazole, imipenem/cilastatin, vancomycin, trimethoprim–sulphamethoxazole (TMP-SMZ) and azithromycin. When the polymerase chain reaction analysis of the bronchoalveolar lavage sample revealed P. jirovecii and A. terreus, all the antibiotics were stopped except TMP-SMZ, and voriconazole was added. Two weeks later, the patient showed clinical improvement but radiological deterioration. Consequently, caspofungin was started for salvage therapy, then the patient showed gradual clinical improvement. He was discharged with oral voriconazole and TMP-SMZ. The antifungal treatment was continued for 6 months until complete radiological absorption. In conclusion, early bronchoscopy with bronchoalveolar lavage fluid should be considered in order to diagnose and treat promptly in those treated with corticosteroids combined with immunocompromised and caspofungin could be an appropriate choice for salvage treatment of coinfection by P. jirovecii and A. terreus.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141258213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Postoperative tumor treatment strategies: From basic research to clinical therapy 肿瘤术后治疗策略：从基础研究到临床治疗

IF 8.6 4区医学

VIEW Pub Date : 2024-05-29 DOI: 10.1002/viw.20230117

Minna Xiao, Lin Wang, Qinglai Tang, Qian Yang, Xinming Yang, Gangcai Zhu, Lanjie Lei, Shisheng Li

{"title":"Postoperative tumor treatment strategies: From basic research to clinical therapy","authors":"Minna Xiao, Lin Wang, Qinglai Tang, Qian Yang, Xinming Yang, Gangcai Zhu, Lanjie Lei, Shisheng Li","doi":"10.1002/viw.20230117","DOIUrl":"https://doi.org/10.1002/viw.20230117","url":null,"abstract":"Despite progression in advanced treatments for malignant tumors, surgery remains the primary treatment intervention, which removes a large portion of firm tumor tissues; however, the postoperative phase poses a possible risk for provincial tumor recurrence and metastasis. Consequently, the prevention of tumor recurrence and metastasis has attracted research attention. In this review, we summarized the postoperative treatment strategies for various tumors from both basic research and clinical perspectives. We delineated the underlying factors contributing to the recurrence of malignant tumors with a substantial prevalence rate, related molecular mechanisms of tumor recurrence post-surgery, and related means of monitoring recurrence and metastasis after surgery. Furthermore, we described relevant therapeutic approaches for postoperative tumor recurrence, including chemotherapy, radiation therapy, immunotherapy, targeted therapy, and photodynamic therapy. This review focused on the emerging technologies used for postoperative tumor treatment in recent years in terms of functional classification, including the prevention of postoperative tumor recurrence, functional reconstruction, and monitoring of recurrence. Finally, we discussed the future development and deficiencies of postoperative tumor therapy. To understand postoperative treatment strategies for tumors from clinical treatment and basic research and further guide the research directions for postoperative tumors.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141192317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid and sensitive diagnosis of live Mycobacterium tuberculosis using clustered regularly interspaced short palindromic repeat‐Cas13a point‐of‐care RNA testing 利用聚类规律性间隔短回文重复-Cas13a 床旁 RNA 检测快速灵敏地诊断活结核分枝杆菌

IF 8.6 4区医学

VIEW Pub Date : 2024-05-15 DOI: 10.1002/viw.20230109

Yu Wang, Huihuang Lin, Anqi Yang, Jiaming Huang, Wei-cong Ren, Jiajun Dong, Shaojie Wang, Wenxue Xu, Yu Pang, Jieming Qu, Jia Liu

{"title":"Rapid and sensitive diagnosis of live Mycobacterium tuberculosis using clustered regularly interspaced short palindromic repeat‐Cas13a point‐of‐care RNA testing","authors":"Yu Wang, Huihuang Lin, Anqi Yang, Jiaming Huang, Wei-cong Ren, Jiajun Dong, Shaojie Wang, Wenxue Xu, Yu Pang, Jieming Qu, Jia Liu","doi":"10.1002/viw.20230109","DOIUrl":"https://doi.org/10.1002/viw.20230109","url":null,"abstract":"Mycobacterium tuberculosis (MTB) is the causal pathogen of tuberculosis (TB). Rapid and accurate detection of live MTB is important for transmission control and patient treatment. Here, we described a clustered regularly interspaced short palindromic repeat (CRISPR)‐Cas13a‐based molecular diagnosis approach for rapid and specific detection of live MTB. This detection method, which we termed CRISPR‐Live‐MTB, contained two consecutive reactions including nuclear acid sequence‐based amplification (NASBA) and CRISPR‐Cas13a collateral cleavage reaction. CRISPR‐Live‐MTB could efficiently detect MTB single‐stranded RNA (ssRNA) in 2 hours with high specificity over double‐stranded DNA (dsDNA). Importantly, CRISPR‐Live‐MTB exhibited a limit of detection of 2.4 copies for MTB ssRNA, which was 1000 times lower than that of the clinically used NASBA method. Moreover, lateral flow was integrated into the CRISPR‐Live‐MTB method to enable point‐of‐care testing application with a sensitivity of 95% and a specificity of 100%. Overall, our study demonstrated the feasibility of CRISPR‐Live‐MTB as a rapid, sensitive, and specific approach for live MTB detection.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140975455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MALDI‐TOF MS analysis of nasal swabs for the characterization of patients infected with SARS‐CoV‐2 Omicron 对鼻拭子进行 MALDI-TOF MS 分析以确定 SARS-CoV-2 Omicron 感染者的特征

IF 8.6 4区医学

VIEW Pub Date : 2024-05-15 DOI: 10.1002/viw.20240015

Rui Song, Dandan Li, Xiaohua Hao, Qian Lyu, Qingwei Ma, Xiaoyou Chen, Liang Qiao

{"title":"MALDI‐TOF MS analysis of nasal swabs for the characterization of patients infected with SARS‐CoV‐2 Omicron","authors":"Rui Song, Dandan Li, Xiaohua Hao, Qian Lyu, Qingwei Ma, Xiaoyou Chen, Liang Qiao","doi":"10.1002/viw.20240015","DOIUrl":"https://doi.org/10.1002/viw.20240015","url":null,"abstract":"With the ongoing mutation of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) leading to various variants, there is an urgent need for new diagnostic methods for SARS‐CoV‐2 infection. The existing nucleic acid test and antigen test suffer from long assay time and low sensitivity, respectively. Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS)‐based nasal swabs analysis have been demonstrated as a promising technique in SARS‐CoV‐2 infection screening. However, the applicability of the technique in the different variants of SARS‐CoV‐2 is uncertain. Given the prevalence of the Omicron variant since 2022, we developed a MALDI‐TOF‐based diagnosis method with nasal swab samples to detect the infection by this variant. We collected 325 SARS‐CoV‐2‐positive and 221 SARS‐CoV‐2‐negative nasal swab samples, and the molecular mass fingerprints were acquired from the samples by MALDI‐TOF MS. Using a random forest machine learning classification model to analyze the molecular mass fingerprints MALDI‐TOF mass spectra, the accuracy of 97%, false negative rate of 0%, and false positive rate of 7.6% were achieved for the diagnosis of SARS‐CoV‐2 infection. Combining the MALDI‐TOF analysis with top‐down proteomics, we identified four potential protein biomarkers, that is, humanin‐like 4, thymosin beta‐10, thymosin beta‐4 and statherin, in the nasal swab for the diagnosis of coronavirus disease 2019. It was further found that the four protein biomarkers can also differentiate the SARS‐CoV‐2 original strains infection and Omicron strains infection. These results suggest that the MALDI‐TOF MS‐based nasal swab analysis holds effective diagnostic capabilities of SARS‐CoV‐2 infection, and shows promising potential for global application and extension to other infectious diseases.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140974311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inside Front Cover: Super-resolution ultrasound localization microscopy for the non-invasive imaging of human testicular microcirculation and its differential diagnosis role in male infertility (View 2/2024) 封面内页：超分辨率超声定位显微镜对人体睾丸微循环的无创成像及其在男性不育症中的鉴别诊断作用（查看2/2024）

IF 8.6 4区医学

VIEW Pub Date : 2024-04-23 DOI: 10.1002/viw2.349

Maoyao Li, Lei Chen, Jipeng Yan, Channa Nalin Jayasena, Zhangshun Liu, Jia Li, Ao Li, Jiang Zhu, Ronghui Wang, Jianchun Li, Chaoxue Zhang, Jingyi Guo, Yuwu Zhao, Chao Feng, Mengxing Tang, Yuanyi Zheng

引用次数: 0

A high-performance cell-labeling NIR-II dye for in vivo cell tracking 用于体内细胞追踪的高性能细胞标记近红外-II 染料

IF 8.6 4区医学

VIEW Pub Date : 2024-04-03 DOI: 10.1002/viw.20230097

Bin Sun, Rui Ma, Xin Wang, Shengjie Ma, Wenzhong Li, Tianyi Liu, Wenhao Zhu, Zhengchao Ji, Kenneth S. Hettie, Chunchen Liu, Yongye Liang, Shoujun Zhu

{"title":"A high-performance cell-labeling NIR-II dye for in vivo cell tracking","authors":"Bin Sun, Rui Ma, Xin Wang, Shengjie Ma, Wenzhong Li, Tianyi Liu, Wenhao Zhu, Zhengchao Ji, Kenneth S. Hettie, Chunchen Liu, Yongye Liang, Shoujun Zhu","doi":"10.1002/viw.20230097","DOIUrl":"https://doi.org/10.1002/viw.20230097","url":null,"abstract":"Fluorescent dyes that emit in the second near-infrared (NIR-II, 1000–3000 nm) region have provided significant advances toward real-time and high-resolution imaging of vessel and lymphatic system. However, in vivo NIR-II tracking of the fate of labeled cells still remains challenging. Here, we develop a shielding unit–donor–acceptor–donor–shielding unit (S-D-A-D-S) NIR-II fluorophore (FE-4ZW) with zwitterionic terminal groups for high-efficiency cell labeling without using cell-penetrating peptides, which provides for enhanced non-invasive in vivo determination of the location of cell migration. The tethering terminal sulfoammonium inner salts are featured with its high affinity for cell membranes, thereby enabling the stable labeling even for fixed cells. The fate of transplanted stem cell and the tumor cell migration along lymphatic system in brain or periphery tissues are clearly monitored by the cell-internalized FE-4ZW. We also confirmed that a clinically used surfactant, D-α-tocopheryl polyethylene glycol-1000 succinate, can reduce the liver and spleen uptake of FE-4ZW. The fluorophore design strategy and cell-labeling technology reported here open a new realm in the visualization of cell migration and insight into the relocation process, thereby ultimately providing an opportunity to investigate in greater detail of the underlying mechanisms of stem cell therapy and tumor metastasis.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140582148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inside Front Cover: Advancements in SERS-based biological detection and its application and perspectives in pancreatic cancer (View 1/2024) 封面内页：基于 SERS 的生物检测进展及其在胰腺癌中的应用和前景（查看 1/2024）

IF 8.6 4区医学

VIEW Pub Date : 2024-02-14 DOI: 10.1002/viw2.339

Lei Xu, Yujiao Xie, Jie Lin, Aiguo Wu, Tianan Jiang

引用次数: 0

Succession of the oral microbiome with the increasing severity of periodontitis 口腔微生物群的继承与牙周炎严重程度的增加

IF 8.6 4区医学

VIEW Pub Date : 2024-02-01 DOI: 10.1002/viw.20230118

Jun Mi, Mengfan Zhi, Wenyan Kang, Qianyu Liang, Di Tang, Ting Wang, Wenjing Song, Tianyong Sun, Meihui Li, Jinlong Shao, Shaohua Ge, Qiang Feng

{"title":"Succession of the oral microbiome with the increasing severity of periodontitis","authors":"Jun Mi, Mengfan Zhi, Wenyan Kang, Qianyu Liang, Di Tang, Ting Wang, Wenjing Song, Tianyong Sun, Meihui Li, Jinlong Shao, Shaohua Ge, Qiang Feng","doi":"10.1002/viw.20230118","DOIUrl":"https://doi.org/10.1002/viw.20230118","url":null,"abstract":"Periodontitis development is strongly associated with the succession of the oral microbiome. However, the knowledge about the succession of the oral microbiome in the development of periodontitis remains insufficient. In the present study, an analysis was conducted on the succession of tongue back, the saliva (Sal) microbiome, and gingival crevicular fluid (GCF) from healthy individuals and patients with mild (CPL), moderate (CPM), severe chronic (CPH), and generalized aggressive periodontitis (GAgP). The composition and structure of the oral microbiome gradually changed with the increasing severity of periodontitis, among which GCF showed the highest correlation with periodontitis. With an ecological preference, pathogens in the mouth varied with the development of periodontitis. In healthy and CPL patients, Sal-derived microorganisms accounted for a large proportion of GCF but exhibited a decrease in patients with CPM, CPH, and GAgP. Permutation and time course sequencing analysis revealed that a variety of microorganisms changed with the severity of periodontitis. A majority of these microorganisms are closely related to clinical periodontal indices. Ecological analysis suggested that the composition of oral microbial communities at different stages of periodontitis is controlled by random processes. The comparison of microbial interaction networks demonstrated that a series of key microorganisms drive oral health to severe periodontitis. In this study, the relationship between the succession process of the oral microbiota and the development of periodontitis was revealed.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139680327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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