IF 8.6 4区医学

VIEW Pub Date : 2023-11-23 DOI: 10.1002/viw.20230063

Sebastian Sittl, Nicolas Helfricht, Georg Papastavrou

{"title":"Contactless calibration of microchanneled AFM cantilevers for fluidic force microscopy","authors":"Sebastian Sittl, Nicolas Helfricht, Georg Papastavrou","doi":"10.1002/viw.20230063","DOIUrl":"https://doi.org/10.1002/viw.20230063","url":null,"abstract":"Atomic force microscopy (AFM) is an analytical technique that is increasingly utilized to determine interaction forces on the colloidal and cellular level. Fluidic force microscopy, also called FluidFM, became a vital tool for biomedical applications. FluidFM combines AFM and nanofluidics by means of a microchanneled cantilever that bears an aperture instead of a tip at its end. Thereby, single colloids or cells can be aspirated and immobilized to the cantilever, for example, to determine adhesion forces. To allow for quantitative measurements, the so-called (inverse) optical lever sensitivity (OLS and InvOLS, respectively) must be determined, which is typically done in a separate set of measurements on a hard, non-deformable substrate. Here, we present a different approach that is entirely based on hydrodynamic principles and does make use of the internal microfluidic channel of a FluidFM-cantilever and an external pressure control. Thereby, a contact-free calibration of the (inverse) optical lever sensitivity (InvOLS) becomes possible in under a minute. A quantitative model based on the thrust equation, which is well-known in avionics, and finite element simulations, is provided to describe the deflection of the cantilever as a function of the externally applied pressure. A comparison between the classical and the here-presented hydrodynamic method demonstrates equal accuracy.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":"28 1","pages":""},"PeriodicalIF":8.6,"publicationDate":"2023-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138539191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A self-evaluated predictive model: A Bayesian neural network approach to colorectal cancer diagnosis 自评估预测模型:贝叶斯神经网络在结直肠癌诊断中的应用

IF 8.6 4区医学

VIEW Pub Date : 2023-11-16 DOI: 10.1002/viw.20230050

Jie Guo, Zihao Wu, Yin Jia, Hongwei Cao, Qin Qin, Tingting Sun, Xinru Zhou, Xue Pan, Cheng Hua, Chuanbin Mao, Shanrong Liu

{"title":"A self-evaluated predictive model: A Bayesian neural network approach to colorectal cancer diagnosis","authors":"Jie Guo, Zihao Wu, Yin Jia, Hongwei Cao, Qin Qin, Tingting Sun, Xinru Zhou, Xue Pan, Cheng Hua, Chuanbin Mao, Shanrong Liu","doi":"10.1002/viw.20230050","DOIUrl":"https://doi.org/10.1002/viw.20230050","url":null,"abstract":"Artificial intelligence has shown immense potential in cancer prediction, but existing models cannot estimate prediction uncertainty by themselves. Here, we developed a Bayesian neural network (BNN) model, BNN‐CRC15, for colorectal cancer (CRC) prediction while assessing its reliability. The model was trained on routine laboratory data obtained from 27,911 participants and provided quantified prediction uncertainty, allowing identification of a subset of participants in which the model was confident, mimicking the diagnostic process of human practitioners. Our model exhibited superior performance (area under the curve = 0.918) in the confident participant group, which accounted for 46.4% of the patients, indicating that routine laboratory data alone are sufficient for accurate predictions in this subset. For the non‐confident group, further advanced tests, such as colonoscopy, could be recommended to achieve more accurate predictions. In addition, our model demonstrated superior overall accuracy (0.848) in all patients, outperforming other five traditional algorithms (extreme gradient boosting, support vector machine, logistic regression, random forest, and artificial neural network) and fecal immunochemical test in distinguishing CRC from non‐CRC. These findings suggest that our BNN‐CRC15 model could serve as a valuable tool for improving CRC diagnosis and prevention.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":"63 1","pages":""},"PeriodicalIF":8.6,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138539108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pancreatic cancer cell line in responsive hydrogel microcapsules for drug evaluation 反应性水凝胶微胶囊用于胰腺癌细胞系的药物评价

IF 8.6 4区医学

VIEW Pub Date : 2023-11-15 DOI: 10.1002/viw.20230048

Taiyu Song, Hui Zhang, Guangling Liu, Yudong Qiu, Huan Wang

{"title":"Pancreatic cancer cell line in responsive hydrogel microcapsules for drug evaluation","authors":"Taiyu Song, Hui Zhang, Guangling Liu, Yudong Qiu, Huan Wang","doi":"10.1002/viw.20230048","DOIUrl":"https://doi.org/10.1002/viw.20230048","url":null,"abstract":"Drug therapies are the cornerstone of systemic treatment for pancreatic cancer patients. However, the relative outcome of drug evaluation is often hampered by the complex microenvironment of pancreatic cancer due to the lack of reasonable tumor models. Here, we proposed a novel platform that integrated pancreatic adenocarcinoma cells encapsulated into hydrogel microcapsules for three-dimensional (3D) tumor cultivation and antitumor agent evaluation. These hydrogel microcapsules contain alginate/poly (<i>N</i>-isopropyl acrylamide) (alginate/PNIPAM) shells and carboxymethyl cellulose cores, which are generated through the microfluidic electrospray technique. The microcapsules have the feature of rapid response to temperature, by which they can regulate the internal pressure environment. Besides, benefiting from good monodispersity, precise size control, and biocompatibility of these microcapsules, these wrapped tumor cells have the capacity for proliferating spontaneously and forming 3D tumor spheroids with good cell viability. We have demonstrated that pancreatic adenocarcinoma cells encapsulated in the composite microcapsules with different PNIPAM concentrations showed different drug sensitivity, which could be ascribed to the influence of external pressures environment. These results indicate that the tumor spheroids coated in these responsive microcapsules have great potential in the analysis of antitumor drug sensitivity.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":"25 1","pages":""},"PeriodicalIF":8.6,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138539109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Visualizing reactive astrocytes: Positron emission tomography imaging ligands for imidazoline‐2 binding sites 可视化活性星形胶质细胞:咪唑啉- 2结合位点的正电子发射断层成像配体

4区医学

VIEW Pub Date : 2023-11-09 DOI: 10.1002/viw.20230037

Wanqing Li, Yuqing Qiao, Jiahui Chen, Yinlong Li, Xiaoyu Du, Steven H. Liang, Hualong Fu

{"title":"Visualizing reactive astrocytes: Positron emission tomography imaging ligands for imidazoline‐2 binding sites","authors":"Wanqing Li, Yuqing Qiao, Jiahui Chen, Yinlong Li, Xiaoyu Du, Steven H. Liang, Hualong Fu","doi":"10.1002/viw.20230037","DOIUrl":"https://doi.org/10.1002/viw.20230037","url":null,"abstract":"Abstract Imidazoline‐2 binding sites (I 2 BS) exhibit specific expression in reactive astrocytes of the brain and are implicated in various pathophysiological processes, including analgesia, inflammation, Alzheimer's disease, Parkinson's disease, and glial tumors. However, the lack of available protein structural data poses a challenge in the exploration of I 2 BS functions and pharmacological characterizations, indicating the need for the discovery of selective ligands. As a non‐invasive and translational molecular imaging tool, positron emission tomography (PET) has found wide application in clinical diagnosis and drug discovery. Consequently, several PET ligands have been developed to “visualize” I 2 BS in the living brain, thereby elucidating the biological implications of I 2 BS and facilitating I 2 BS‐directed drug development. This review offers a comprehensive update on I 2 BS PET ligands, with a focus on their chemotypes and PET imaging outcomes. Furthermore, the review provides a summary of recent I 2 BS drug discoveries, which could serve as a catalyst for the development of more potent PET ligands.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":" 11","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135290525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Portable non‐enzymatic electrochemical biosensor based on caffeine for Alzheimer's disease diagnosis 基于咖啡因的便携式非酶电化学生物传感器用于阿尔茨海默病诊断

4区医学

VIEW Pub Date : 2023-10-13 DOI: 10.1002/viw.20230052

Xindan Zhang, Audrey Wang, Chaojie Wang, Xiaoying Tang, Zhenqi Jiang, Jieling Qin

{"title":"Portable non‐enzymatic electrochemical biosensor based on caffeine for Alzheimer's disease diagnosis","authors":"Xindan Zhang, Audrey Wang, Chaojie Wang, Xiaoying Tang, Zhenqi Jiang, Jieling Qin","doi":"10.1002/viw.20230052","DOIUrl":"https://doi.org/10.1002/viw.20230052","url":null,"abstract":"Abstract Caffeine and its derivatives can effectively bind amyloid beta 16–22 (Aβ 16–22 ) fragment of amyloid beta 1–42 (Aβ 1–42 ), a biomarker for the early diagnosis of Alzheimer's disease (AD), by means of conformation selection, π–π stacking, van der Waals forces, and hydrogen bonding, so as to achieve high specificity and quantitative detection of Aβ 1–42 . In this study, 3‐mercaptopropionic acid (MPA), conductive polymer poly(thiophene‐3‐acetic acid) (PTAA), and pine‐like/Au pine PTAA (pine PTAA) were applied to modify the electrodes, and the non‐enzymatic caffeine was used as specific biorecognition element to study the analytical performance of the electrochemical sensor platform for Aβ 1–42 oligomer (AβO). It was found that caffeine/pine PTAA‐based sensor with large surface area, high active sites, and excellent electrical conductivity demonstrated the widest linear range (10 −8 to 100 nM) and highest sensitivity (743.77 Ω/log nM) in comparison. The prepared caffeine‐based sensor was afterward applied to cerebrospinal fluid and blood tests for real sample analysis, demonstrating its potential for practical use in detecting AβO at the attomolar level. Furthermore, the non‐enzymatic caffeine was constructed on the pine‐like PTAA‐modified screen‐printed electrodes for the rapid detection of AβO using portable meter.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135854548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alginate supramolecular hydrogels based on viologen and cucurbit[8]uril: Host‐induced caveolae‐mediated endocytosis to white blood cancer cells 基于紫紫素和葫芦素的海藻酸盐超分子水凝胶[8]。宿主诱导的小泡介导的白细胞内吞作用

4区医学

VIEW Pub Date : 2023-10-13 DOI: 10.1002/viw.20230058

Falguni Chandra, Maria Bykova, Vijo Poulose, Paltan Laha, Alina Aktanova, Olga Boeva, Margarita Barkovskaya, Ekaterina Pashkina, Na'il Saleh

{"title":"Alginate supramolecular hydrogels based on viologen and cucurbit[8]uril: Host‐induced caveolae‐mediated endocytosis to white blood cancer cells","authors":"Falguni Chandra, Maria Bykova, Vijo Poulose, Paltan Laha, Alina Aktanova, Olga Boeva, Margarita Barkovskaya, Ekaterina Pashkina, Na'il Saleh","doi":"10.1002/viw.20230058","DOIUrl":"https://doi.org/10.1002/viw.20230058","url":null,"abstract":"Abstract The cellular uptake of drug carriers to the cytosol of a specific cell remains challenging, and a non‐classical supramolecular strategy is motivated. Here, we select a model host–guest complex in which a diamino‐viologen (1,1′‐bis(4‐aminophenyl)‐[4,4′‐bipyridine]−1,1′‐diium dichloride [VG]) fluorescent tag was engulfed by cucurbit[8]uril (CB8) and covalently linked to alginate polysaccharides (alginic acid [ALG]) as the modified drug vehicle. When adsorbed on the ALG surface, the encapsulation of VG was first confirmed utilizing Fourier transform infrared and nuclear magnetic resonance spectroscopic methods. Solid optical measurements (diffuse reflectance spectroscopy, photoluminescence, and time‐resolved photoluminescence) revealed emissive materials at around 650 nm and that CB8 enhanced the rigidity of the modified hydrogel. The molar composition of 2:1 for the complexation of VG to CB8 on the alginate surface and the thermal stabilities were also confirmed using thermogravimetric analysis and differential scanning calorimetry techniques. CB8 induced a dramatic decrease in the average size of the VGALG polysaccharides from 485 to 165 nm and a turnover in their charge from –19.8 to +14.4 mV. Flow cytometry with inhibitors of various endocytosis pathways was employed to track the cellular uptake across different blood cell types: human T‐cell leukemia 1301 and peripheral blood mononuclear cells. Noticeably, complexation of VG with the CB8 host on top of the sugar platform dramatically enhanced the internalization into 1301 cells (viz. from 1% to 99%) at a concentration of 1.8 mg/mL via caveolae‐mediated endocytosis because of the size reduction, turnover in the charge from negative to positive, and rigidity induction. These observations reveal a more profound understanding of the macrocyclic effects on drug delivery.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135853496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Back Cover: Value of [⁶⁸Ga]Ga‐FAPI‐04 PET imaging in acute coronary syndrome complicated by suspected gastrointestinal malignancies (View 5/2023) [68Ga]Ga‐FAPI‐04 PET成像在急性冠状动脉综合征合并疑似胃肠道恶性肿瘤中的价值(View 5/2023)

4区医学

VIEW Pub Date : 2023-10-01 DOI: 10.1002/viw2.317

Mingzhen Ying, Qinqin Yang, Xudong Xu, Shengyong Wu, Wei Yin, Siyu Liang, Guixia Pan, Changjing Zuo, Zhifu Guo, Chao Cheng, Suxuan Liu

引用次数: 0

The application advances of dendrimers in biomedical field 树状大分子在生物医学领域的应用进展

4区医学

VIEW Pub Date : 2023-09-18 DOI: 10.1002/viw.20230023

Longjie Li, Yukai Deng, Yonghui Zeng, Bei Yan, Yulian Deng, Ziyang Zheng, Siqi Li, Yuhang Yang, Jinwei Hao, Xianjin Xiao, Xinyu Wang

引用次数: 0

Back Cover: CBT‐Cys click reaction for optical bioimaging in vivo (View 4/2023) 封底:体内光学生物成像的CBT‐Cys点击反应(View 4/2023)