VIEW最新文献 - Book学术

Frontiers in artificial intelligence-directed light-sheet microscopy for uncovering biological phenomena and multiorgan imaging 用于揭示生物现象和多器官成像的人工智能定向光片显微镜的前沿领域

IF 8.6 4区医学

VIEW Pub Date : 2024-09-03 DOI: 10.1002/viw.20230087

Enbo Zhu, Yan-Ruide Li, Samuel Margolis, Jing Wang, Kaidong Wang, Yaran Zhang, Shaolei Wang, Jongchan Park, Charlie Zheng, Lili Yang, Alison Chu, Yuhua Zhang, Liang Gao, Tzung K. Hsiai

{"title":"Frontiers in artificial intelligence-directed light-sheet microscopy for uncovering biological phenomena and multiorgan imaging","authors":"Enbo Zhu, Yan-Ruide Li, Samuel Margolis, Jing Wang, Kaidong Wang, Yaran Zhang, Shaolei Wang, Jongchan Park, Charlie Zheng, Lili Yang, Alison Chu, Yuhua Zhang, Liang Gao, Tzung K. Hsiai","doi":"10.1002/viw.20230087","DOIUrl":"https://doi.org/10.1002/viw.20230087","url":null,"abstract":"Light-sheet fluorescence microscopy (LSFM) introduces fast scanning of biological phenomena with deep photon penetration and minimal phototoxicity. This advancement represents a significant shift in 3-D imaging of large-scale biological tissues and 4-D (space + time) imaging of small live animals. The large data associated with LSFM require efficient imaging acquisition and analysis with the use of artificial intelligence (AI)/machine learning (ML) algorithms. To this end, AI/ML-directed LSFM is an emerging area for multiorgan imaging and tumor diagnostics. This review will present the development of LSFM and highlight various LSFM configurations and designs for multiscale imaging. Optical clearance techniques will be compared for effective reduction in light scattering and optimal deep-tissue imaging. This review will further depict a diverse range of research and translational applications, from small live organisms to multiorgan imaging to tumor diagnosis. In addition, this review will address AI/ML-directed imaging reconstruction, including the application of convolutional neural networks (CNNs) and generative adversarial networks (GANs). In summary, the advancements of LSFM have enabled effective and efficient post-imaging reconstruction and data analyses, underscoring LSFM's contribution to advancing fundamental and translational research.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142205090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exosomes of immune cell origin and their therapeutic potential for tumors 免疫细胞外泌体及其对肿瘤的治疗潜力

IF 8.6 4区医学

VIEW Pub Date : 2024-09-02 DOI: 10.1002/viw.20240029

Hongru Ai, Yitan Zou, Xinya Zheng, Guangyao Li, Changhai Lei, Wenyan Fu, Shi Hu

引用次数: 0

Optical imaging for brown or beige adipose tissue 棕色或米色脂肪组织的光学成像

IF 8.6 4区医学

VIEW Pub Date : 2024-08-23 DOI: 10.1002/viw.20240022

Jiamin Liu, Linjie Ni, Minmin Peng, Yiying Liang, Chan Lu, Hanying Zheng, Zicheng Huang, Jinde Zhang, Ronghe Chen

{"title":"Optical imaging for brown or beige adipose tissue","authors":"Jiamin Liu, Linjie Ni, Minmin Peng, Yiying Liang, Chan Lu, Hanying Zheng, Zicheng Huang, Jinde Zhang, Ronghe Chen","doi":"10.1002/viw.20240022","DOIUrl":"https://doi.org/10.1002/viw.20240022","url":null,"abstract":"Currently, one in three adults worldwide may be obese or overweight. Obesity is characterized by an excess of energy-storing white fat. An ingenious and sought-after strategy against obesity is to activate energy-consuming beige fat, which is converted from white fat or brown fat. However, existing tools for assessing brown or beige fat activation in vivo have certain limitations, such as being cumbersome and expensive. Optical imaging is a relatively straightforward and economical imaging technique that utilizes light to peer into the structural–functional information of living organisms at multiple scales. Despite the availability of various optical imaging modalities for detecting brown or beige fat, there is a dearth of literature summarizing relevant studies. Accordingly, this review focuses on these optical modalities and elaborates on their imaging principles, characteristics, and recent research advances in the detection of brown or beige fat. Their imaging targets, advantages, and disadvantages are further concluded. As a methodological reference, this review can guide the selection of optimal optical modalities to noninvasively profile brown or beige fat activation from a specific biological perspective, maximizing the potential of optical imaging in anti-obesity assessment.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142205144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical detection of the cardiac biomarker cardiac troponin I 心脏生物标记物心肌肌钙蛋白 I 的电化学检测

IF 8.6 4区医学

VIEW Pub Date : 2024-08-22 DOI: 10.1002/viw.20240025

Xiaoyun Qin, Dongyang Li, Xiaomei Qin, Fenghua Chen, Huishi Guo, Yanghai Gui, Jianbo Zhao, Liying Jiang, Dan Luo

{"title":"Electrochemical detection of the cardiac biomarker cardiac troponin I","authors":"Xiaoyun Qin, Dongyang Li, Xiaomei Qin, Fenghua Chen, Huishi Guo, Yanghai Gui, Jianbo Zhao, Liying Jiang, Dan Luo","doi":"10.1002/viw.20240025","DOIUrl":"https://doi.org/10.1002/viw.20240025","url":null,"abstract":"Acute myocardial infarction (AMI) is a major cause of cardiovascular disease-related death. It is essential for patients with cardiovascular disease to receive an early diagnosis of AMI. The most popular technique for the early detection of AMI is the use of biosensors to monitor the concentration of pertinent biomarkers, such as cardiac troponin I (cTnI), in the blood. The electrochemical detection methods hold great promise because of their simplicity, miniaturization, ease of integration, high sensitivity, and rapid response. The prime motive of this review is to present a comprehensive understanding of the pros and cons of methodologies employed for the electrochemical approaches toward the detection of cTnI. A detailed summary is provided for the immunosensors, aptamer sensors, molecular imprinting sensors, and peptide sensors based on various affinity elements. We enumerate the modified electrode materials for electrochemical sensors as well as popular detection techniques. Furthermore, this paper reviews some recent significant advances in point-of-care assays for rapid, accurate detection of cTnI as a smart integrated device for home monitoring. The accumulation of knowledge about these functions will lead to new insights into and concepts for the design of portable miniature sensors for cardiovascular patients at risk of AMI. It is anticipated that the interdisciplinary collaboration can bring more enlightenment to the progress of cardiac biomarkers sensor in the future.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142205143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantification of cellular phototoxicity of organelle stains by the dynamics of microtubule polymerization 通过微管聚合动态量化细胞器染色剂的细胞光毒性

IF 8.6 4区医学

VIEW Pub Date : 2024-08-22 DOI: 10.1002/viw.20240013

Shivam Mahapatra, Seohee Ma, Bin Dong, Chi Zhang

{"title":"Quantification of cellular phototoxicity of organelle stains by the dynamics of microtubule polymerization","authors":"Shivam Mahapatra, Seohee Ma, Bin Dong, Chi Zhang","doi":"10.1002/viw.20240013","DOIUrl":"https://doi.org/10.1002/viw.20240013","url":null,"abstract":"Being able to quantify the phototoxicity of dyes and drugs in live cells allows biologists to better understand cell responses to exogenous stimuli during imaging. This capability further helps to design fluorescent labels with lower phototoxicity and drugs with better efficacy. Conventional ways to evaluate cellular phototoxicity rely on late-stage measurements of individual or different populations of cells. Here, we developed a quantitative method using intracellular microtubule polymerization as a rapid and sensitive marker to quantify early-stage phototoxicity. Implementing this method, we assessed the photosensitization induced by organelle dyes illuminated with different excitation wavelengths. Notably, fluorescent markers targeting mitochondria, nuclei, and endoplasmic reticulum exhibited diverse levels of phototoxicity. Furthermore, leveraging a real-time precision opto-control technology allowed us to evaluate the synergistic effect of light and dyes on specific organelles. Studies in hypoxia revealed enhanced phototoxicity of Mito-Tracker Red CMXRos that is not correlated with the generation of reactive oxygen species but a different deleterious pathway in low oxygen conditions.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142226269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrogels loaded with MSC-derived small extracellular vesicles: A novel cell-free tissue engineering system for diabetic wound management 含有间充质干细胞衍生小细胞外囊泡的水凝胶：用于糖尿病伤口管理的新型无细胞组织工程系统

IF 8.6 4区医学

VIEW Pub Date : 2024-07-18 DOI: 10.1002/viw.20230110

Weicheng Zhong, Hao Meng, Liqian Ma, Xizi Wan, Shengqiu Chen, Kui Ma, Lu Lu, Jianlong Su, Kailu Guo, Yufeng Jiang, Xi Liu, Xiaobing Fu, Cuiping Zhang

{"title":"Hydrogels loaded with MSC-derived small extracellular vesicles: A novel cell-free tissue engineering system for diabetic wound management","authors":"Weicheng Zhong, Hao Meng, Liqian Ma, Xizi Wan, Shengqiu Chen, Kui Ma, Lu Lu, Jianlong Su, Kailu Guo, Yufeng Jiang, Xi Liu, Xiaobing Fu, Cuiping Zhang","doi":"10.1002/viw.20230110","DOIUrl":"https://doi.org/10.1002/viw.20230110","url":null,"abstract":"With the aging and obesity era, the increasing incidence of diabetes and diabetic complications, especially the non-healing wounds, imposes a serious economic burden on both patients and society. The complex microenvironments, including hyperglycemia, bacterial infection, ischemia, and nerve damage, lead to the prolonged inflammation and proliferation phase of diabetic wounds. Mesenchymal stem cell-derived small extracellular vesicles (MSC-sEVs), which contain a rich variety of therapeutic molecules, have been chased for decades because of their potential roles in cellular communication, tissue regeneration, and drug delivery. As powerful tools for the controlled-sustained release of sEVs, biocompatible hydrogels have been applied in a wide range of biomedical applications. Herein, we first summarize the pathological features of diabetic wounds, such as angiopathy, neuropathy, and immune cell dysfunction. Then, we discuss the biological properties, therapeutic performance, and stability of pure MSC-sEVs. After that, we discuss the components, application patterns, and responsiveness of hydrogels. Next, we discuss the loading avenues of MSC-sEVs into hydrogel, the release behaviors of sEVs from hydrogels, and the influence of the crosslinking method on the hydrogel-sEV composites. Finally, we provide an overview of the current applications of hydrogels loaded with MSC-sEVs as a novel cell-free tissue engineering system in managing diabetic wounds and propose the critical unsolved issues. This review is expected to provide meaningful guidance for developing a novel cell-free tissue engineering system for diabetic wound management.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141738512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep learning-enhanced microwell array biochip for rapid and precise quantification of Cryptococcus subtypes 用于快速精确量化隐球菌亚型的深度学习增强型微孔阵列生物芯片

IF 8.6 4区医学

VIEW Pub Date : 2024-07-18 DOI: 10.1002/viw.20240032

Yihang Tong, Yu Zeng, Yinuo Lu, Yemei Huang, Zhiyuan Jin, Zhiying Wang, Yusen Wang, Xuelei Zang, Lingqian Chang, Wei Mu, Xinying Xue, Zaizai Dong

{"title":"Deep learning-enhanced microwell array biochip for rapid and precise quantification of Cryptococcus subtypes","authors":"Yihang Tong, Yu Zeng, Yinuo Lu, Yemei Huang, Zhiyuan Jin, Zhiying Wang, Yusen Wang, Xuelei Zang, Lingqian Chang, Wei Mu, Xinying Xue, Zaizai Dong","doi":"10.1002/viw.20240032","DOIUrl":"https://doi.org/10.1002/viw.20240032","url":null,"abstract":"Cryptococcus is a family of strongly infectious pathogens that results in a wide variety of symptoms, particularly threatening the patients undergoing the immune-deficiency or medical treatment. Rapidly identifying Cryptococcus subtypes and accurately quantifying their contents remain urgent needs for infection control and timely therapy. However, traditional detection techniques heavily rely on expensive, specialized instruments, significantly compromising their applicability for large-scale population screening. In this work, we report a portable microwell array chip platform integrated with a deep learning-based image recognition program, which enables rapid, precise quantification of the specific subtypes of Cryptococcus. The platform features four zones of microwell arrays preloaded with the subtype-targeted CRISPR–Cas12a system that avoid dependence on slow, instrumental-mediated target amplification, achieving rapid (10 min), high specificity for identifying the sequence of Cryptococcus. The deep learning-based image recognition program utilizing segment anything model (SAM) significantly enhances automation and accuracy in identifying target concentrations, which eventually achieves ultra-low limit of detection (0.5 pM) by personal smartphones. This platform can be further customized to adapt to various scenarios in clinical settings.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141753964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oleic and linoleic acids induce oxidative stress in chondrocytes by inhibiting autophagy-regulated ciliogenesis 油酸和亚油酸通过抑制自噬调控的纤毛生成诱导软骨细胞氧化应激

IF 8.6 4区医学

VIEW Pub Date : 2024-06-28 DOI: 10.1002/viw.20240019

Xiaodong Li, Chuanyuan Mao, Guantong Sun, Chen Zhao, Jingjing Dai, Fei Yang, Lei Wang, Xiaoqing Wang

{"title":"Oleic and linoleic acids induce oxidative stress in chondrocytes by inhibiting autophagy-regulated ciliogenesis","authors":"Xiaodong Li, Chuanyuan Mao, Guantong Sun, Chen Zhao, Jingjing Dai, Fei Yang, Lei Wang, Xiaoqing Wang","doi":"10.1002/viw.20240019","DOIUrl":"https://doi.org/10.1002/viw.20240019","url":null,"abstract":"The lack of a cure for osteoarthritis (OA), a prevalent joint disease among older individuals, remains an ongoing challenge. Obesity is a common risk factor for OA. Chondrocyte autophagy plays a crucial role in delaying the onset of OA. Our previous studies have demonstrated a significant elevation in the levels of oleic acid (OLA) and linoleic acid (LA) in the synovial fluid (SF) of patients with OA and obesity compared to those with OA alone, and an inhibitory effect of these molecules on the activation of autophagy. Accumulating evidence indicates a reciprocal regulatory relationship between autophagy and ciliogenesis; however, whether autophagy-mediated ciliogenesis plays a significant role in the pathogenesis of OA remains unclear. In this study, we aimed to determine whether OLA and LA affect OA development via the regulation of chondrocyte autophagy-mediated ciliogenesis. We found that both molecules inhibited this process in chondrocytes. Moreover, intracellular calcium and reactive oxygen species (ROS) levels increased simultaneously. Further, we explored the relationship between autophagy and ciliogenesis in chondrocytes. Activation of autophagy by rapamycin significantly attenuated the ciliogenesis inhibition caused by OLA and LA. Importantly, the downregulation of AKT and mTOR expression in chondrocytes reversed the autophagy-mediated inhibition of ciliogenesis and the ROS-accumulation-mediated inflammation induced by OLA and LA. Taken together, our results suggest that OLA and LA induce calcium-overload-driven ROS accumulation via autophagy-mediated ciliogenic disorders during OA pathogenesis. These findings demonstrate that targeting autophagy and ciliogenesis in chondrocytes is a protective strategy in the OA pathogenesis induced by OLA and LA.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141510500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photoacoustic imaging in brain disorders: Current progress and clinical applications 脑部疾病的光声成像：当前进展与临床应用

IF 8.6 4区医学

VIEW Pub Date : 2024-06-27 DOI: 10.1002/viw.20240023

Xiuyun Liu, Haodong Li, Meijun Pang, Jinzhen Liu, Xizi Song, Runnan He, Mengqi He, Xiqi Jian, Cheng Ma, Handi Deng, Yixuan Wu, Dong Ming

{"title":"Photoacoustic imaging in brain disorders: Current progress and clinical applications","authors":"Xiuyun Liu, Haodong Li, Meijun Pang, Jinzhen Liu, Xizi Song, Runnan He, Mengqi He, Xiqi Jian, Cheng Ma, Handi Deng, Yixuan Wu, Dong Ming","doi":"10.1002/viw.20240023","DOIUrl":"https://doi.org/10.1002/viw.20240023","url":null,"abstract":"Over the past few decades, the number of patients with neurological diseases has increased significantly, posing huge challenges and opportunities for the development of brain imaging technology. As a hybrid imaging method combining optical excitation and acoustic detection techniques, photoacoustic tomography (PAT), has experienced rapid development, due to high optical contrast and spatial resolution at depth inside tissues. With the development of lasers, ultrasonic detectors, and data computations, PAT has been widely applied for the diagnosis of oncology, dermatosis, etc. However, the energy of light and ultrasound would be greatly attenuated while penetrating the skull, due to the reflection, absorption, and scattering effects, resulting in limited application of PAT in brain imaging. In this review, we summarized the achievements of PAT and its application in the detection of brain diseases including glioma, stroke, traumatic brain injury, Alzheimer's disease, epilepsy, and Parkinson's disease. Moreover, various PAT systems and multi-modality photoacoustic imaging are introduced for potential clinical applications. Finally, the challenges and current limitations of PAT for further brain imaging are also discussed.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141510501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-material direct-ink-writing of silver-based flexible and highly deformable dry electrocardiogram biopatches 以多种材料直接墨水书写银基柔性高变形干式心电图生物贴片

IF 8.6 4区医学

VIEW Pub Date : 2024-06-26 DOI: 10.1002/viw.20240008

Aljawharah A. Alsharif, Jesus M. Aviles, Felipe M. Zechel, Nouf A. Alsharif, Nazek El-Atab

{"title":"Multi-material direct-ink-writing of silver-based flexible and highly deformable dry electrocardiogram biopatches","authors":"Aljawharah A. Alsharif, Jesus M. Aviles, Felipe M. Zechel, Nouf A. Alsharif, Nazek El-Atab","doi":"10.1002/viw.20240008","DOIUrl":"https://doi.org/10.1002/viw.20240008","url":null,"abstract":"Of significant interest are three-dimensional (3D) printed dry electrodes, a departure from traditional wet silver/silver chloride (Ag/AgCl) electrodes. These innovative electrodes not only incorporate 3D printed personalized materials but also eliminate the need for electrolyte gel, which tends to dehydrate over time. Additionally, these electrodes boast unique attributes such as stretchability, deformability, biocompatibility, wearable comfort, and cost-effective manufacturing. While the advantages of dry electrodes are apparent, their performance optimization encounters challenges related to charge migration, particularly when scaled down to miniaturized dimensions, impacting biosignal detection. This study addresses these challenges by focusing on the development of scalable, stretchable, and highly deformable syringe-printed dry electrocardiogram (ECG) patches. The approach employs straightforward multi-material direct-ink-writing (DIW) techniques, realizing complete biopatches per print, resulting in a rapid and cost-effective fabrication process. The achieved printing resolution reaches up to 200 µm, and the conductivity of Ag/AgCl dry electrodes reaches approximately ∼ 5 × 104 S/m. This not only ensures scalability but also expands the applications of metal-based inks to various soft electronic devices, particularly in low-resource settings and environments.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141532230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0