Super-resolution ultrasound localization microscopy for the non-invasive imaging of human testicular microcirculation and its differential diagnosis role in male infertility

IF 9.7 4区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS
VIEW Pub Date : 2024-01-11 DOI:10.1002/viw.20230093
Maoyao Li, Lei Chen, Jipeng Yan, Channa Nalin Jayasena, Zhangshun Liu, Jia Li, Ao Li, Jiang Zhu, Ronghui Wang, Jianchun Li, Chaoxue Zhang, Jingyi Guo, Yuwu Zhao, Chao Feng, Mengxing Tang, Yuanyi Zheng
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引用次数: 0

Abstract

Testicular microcirculation is closely related to spermatogenic function and seminiferous tubular function. The diagnosis and monitoring of testicular diseases can be associated with testicular microcirculation; however, there are currently no effective non-invasive methods for super-resolution imaging of testicular microcirculation. In this study, we introduced state-of-the-art graph-based tracking with the Kalman motion model algorithm to non-invasively image human testicular microcirculation for the first time with a regular frame-rate clinical ultrasound imaging system (37 Hz). Two distinct testicular vessels with an 81 µm separation were resolved in the testicular vasculature, surpassing all other imaging modalities. In a retrospective study, we performed contrast-enhanced ultrasound examinations(CEUS) and ultrasound localization microscopy (ULM) processing on the included 76 infertile patients and 15 healthy controls from August 2021 to May 2023 and obtained super-resolution images of testicular microcirculation with sub-diffraction resolution. Through the results of one-way analysis of variance tests and receiver operating characteristic analyses, we found that the ULM-based parameters hold promise as clinical guidance for differentiating between non-obstructive and obstructive male infertility. The mean vessel diameter achieved an area under the curve (AUC) of 0.920 (95% confidence interval [CI]: 0.847–0.994, p < .001) with a cut-off value of 170.9 µm in oligoasthenospermia, and an AUC of 0.952 (95% CI: 0.875–1.000, p < .001) with a cut-off value of 169.9 µm in azoospermia patients, respectively, addressing a significant clinical challenge.

Abstract Image

超分辨率超声定位显微镜对人体睾丸微循环的无创成像及其在男性不育症中的鉴别诊断作用
睾丸微循环与生精功能和曲细精管功能密切相关。睾丸疾病的诊断和监测可能与睾丸微循环有关;然而,目前还没有有效的无创方法对睾丸微循环进行超分辨率成像。在这项研究中,我们采用了最先进的基于图的卡尔曼运动模型跟踪算法,首次利用常规帧频的临床超声成像系统(37 Hz)对人体睾丸微循环进行了无创成像。在睾丸血管中分辨出了两条不同的睾丸血管,它们之间的距离为 81 微米,超越了所有其他成像模式。在一项回顾性研究中,我们从2021年8月至2023年5月对76名不育患者和15名健康对照者进行了对比增强超声检查(CEUS)和超声定位显微镜(ULM)处理,获得了亚衍射分辨率的睾丸微循环超分辨率图像。通过单向方差分析和接收器操作特性分析,我们发现基于超分辨微循环的参数有望作为区分非梗阻性和梗阻性男性不育症的临床指导。在少精子症患者中,以 170.9 µm 为临界值,平均血管直径的曲线下面积 (AUC) 为 0.920(95% 置信区间 [CI]:0.847-0.994,p < .001);在无精子症患者中,以 169.9 µm 为临界值,平均血管直径的曲线下面积 (AUC) 为 0.952(95% 置信区间 [CI]:0.875-1.000,p < .001)。
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来源期刊
VIEW
VIEW Multiple-
CiteScore
12.60
自引率
2.30%
发文量
0
审稿时长
10 weeks
期刊介绍: View publishes scientific articles studying novel crucial contributions in the areas of Biomaterials and General Chemistry. View features original academic papers which go through peer review by experts in the given subject area.View encourages submissions from the research community where the priority will be on the originality and the practical impact of the reported research.
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