Food and Chemical Toxicology最新文献

{"title":"Toward a stronger evidence base for heavy metal mixtures and prostate cancer risk","authors":"Yuekun Fang , Shengyi Chen , Bin Cheng","doi":"10.1016/j.fct.2025.115757","DOIUrl":"10.1016/j.fct.2025.115757","url":null,"abstract":"","PeriodicalId":317,"journal":{"name":"Food and Chemical Toxicology","volume":"206 ","pages":"Article 115757"},"PeriodicalIF":3.5,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145135980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Update to RIFM fragrance ingredient safety assessment, 2-hydroxyacetophenone, CAS Registry Number CAS # 118-93-4","authors":"A.M. Api , A. Bartlett , D. Belsito , D. Botelho , M. Bruze , A. Bryant-Friedrich , G.A. Burton Jr. , M.A. Cancellieri , H. Chon , M. Cronin , S. Crotty , M.L. Dagli , W. Dekant , C. Deodhar , K. Farrell , A.D. Fryer , L. Jones , K. Joshi , A. Lapczynski , D.L. Laskin , Y. Thakkar","doi":"10.1016/j.fct.2025.115753","DOIUrl":"10.1016/j.fct.2025.115753","url":null,"abstract":"","PeriodicalId":317,"journal":{"name":"Food and Chemical Toxicology","volume":"206 ","pages":"Article 115753"},"PeriodicalIF":3.5,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145124088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary prebiotics combined with iron supplementation: A two-pronged approach to reduce rice cadmium bioavailability","authors":"Rong-Yue Xue ,&nbsp;Yao-Sheng Zhang ,&nbsp;Jin-Feng Xi,&nbsp;Lei Zhou,&nbsp;Xin-Ying Lin,&nbsp;Tian-Wen Ye,&nbsp;Yu-Hang Bao,&nbsp;Dongmei Zhou,&nbsp;Hong-Bo Li","doi":"10.1016/j.fct.2025.115745","DOIUrl":"10.1016/j.fct.2025.115745","url":null,"abstract":"<div><div>While the role of improving gut microbiota and elevating dietary iron (Fe) in reducing cadmium (Cd) oral bioavailability has been assessed, the role of dietary prebiotics combined with Fe in synergistically reducing Cd bioavailability from rice remains underexplored. This study utilized mouse bioassays to investigate the potential of prebiotics alone or combined with Fe in reducing rice Cd bioavailability. In experiments with mice fed Fe-sufficient diets (60.6 μg g⁻¹) supplemented with Cd, dietary fructooligosaccharides (FOS), galactooligosaccharides (GOS), and inulin (at 5 % w/w) enhanced Fe absorption and reduced Cd uptake. However, in mice consuming Cd-contaminated rice with low Fe content (6.81 μg g⁻¹), these prebiotics did not increase Fe absorption but lowered mouse kidney Cd by 12.1–20.7 % by improving gut microbiota. Combining 5 % GOS with 100 μg Fe g⁻¹ in Cd-contaminated rice diets resulted in a 59 % reduction in kidney Cd accumulation, more effective than the reduction with either GOS (8.66 %) or Fe (31.9 %) alone, due to the combination of the role of GOS in improving gut microbiota and enhancing intestinal barrier functions and the role of Fe in lowering intestinal Fe transporters. These findings propose a combined dietary strategy to efficiently mitigate the health risks associated with rice Cd exposure.</div></div>","PeriodicalId":317,"journal":{"name":"Food and Chemical Toxicology","volume":"206 ","pages":"Article 115745"},"PeriodicalIF":3.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145099561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heavy metals and metalloids in selected Mood Foods in Poland: Development of sample preparation and analysis methods and assessment of the health risks of consumption","authors":"Dorota Jakkielska ,&nbsp;Barbara Topolska ,&nbsp;Joanna Wolska ,&nbsp;Iwona Kurzyca ,&nbsp;Marcin Frankowski ,&nbsp;Anetta Zioła-Frankowska","doi":"10.1016/j.fct.2025.115746","DOIUrl":"10.1016/j.fct.2025.115746","url":null,"abstract":"<div><div>The subject of the study are Mood Foods, food products that improve well-being and are widely recognized as healthy, but just like other food products, they could be sources of heavy metals in human diet. Elements, such as mercury, lead, cadmium and arsenic, which have neurotoxic effects, can be found in food as a result of air, soil, and underground and groundwater pollution. Therefore, the study aimed to (1) develop and optimize universal sample preparation and ICP-MS analysis methods with high repeatability and recovery, (2) qualitatively and quantitatively analyze arsenic, cadmium, mercury and lead contents in various mood food products – hazelnuts, instant oat flakes, roasted buckwheat groats, salmon, tuna, spinach, dark chocolate, rice and green tea, and (3) describe the obtained results and assess the health risk – by calculating Estimated Daily Intake, percentage of Provisional Tolerable Weekly Intake, Target Hazard Quotient, Hazard Index, and Carcinogenic Risk – due to consumption of foods containing elements at determined content levels: As: 3.429–4745.289 μg kg⁻¹, Cd: 3.583–721.572 μg kg⁻¹, Hg: 0.355–3044.705 μg kg⁻¹, and Pb: 12.614–1246.174 μg kg⁻¹. It has been shown that regular consumption of certain mood food products could pose non-carcinogenic and carcinogenic health risk to consumers.</div></div>","PeriodicalId":317,"journal":{"name":"Food and Chemical Toxicology","volume":"206 ","pages":"Article 115746"},"PeriodicalIF":3.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145109129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New microplate disc volatilization assay for in vitro evaluation of toxicity and antimicrobial activity of volatiles in vapour phase","authors":"Marketa Houdkova ,&nbsp;Lucie Vistejnova ,&nbsp;Julien Antih ,&nbsp;Aishwarya Chaure ,&nbsp;Iveta Paurova ,&nbsp;Mukund Lal Naik ,&nbsp;Pavel Novy ,&nbsp;Pavel Klein ,&nbsp;Manas Kanti Deb ,&nbsp;Ladislav Kokoska","doi":"10.1016/j.fct.2025.115744","DOIUrl":"10.1016/j.fct.2025.115744","url":null,"abstract":"<div><div>In this study, a novel microplate disc volatilization method for <em>in vitro</em> screening of cytotoxic and antibacterial potential of volatile agents in the vapour phase was designed with the aim to assess their therapeutic potential for development of new inhalation therapies. The toxicity and antibacterial activity of plant-derived volatiles (allyl isothiocyanate, 8-quinolinol, thymohydroquinone, thymoquinone) and essential oils (EOs) from <em>Colebrookea oppositifolia</em> leaf and <em>Neolitsea cassia</em> bark were evaluated against human lung fibroblasts MRC-5 and respiratory pathogens. Allyl isothiocyanate and <em>C. oppositifolia</em> EO did not affect the cell viability (IC₅₀ &gt; 160 μg/cm³), on the other hand thymohydroquinone exhibited the highest cytotoxic effect (IC₅₀ = 5.85 μg/cm³) followed by thymoquinone and <em>N. cassia</em> EO (IC₅₀ = 7.95 and 18.58 μg/cm³, respectively). 8-Quinolinol possessed the strongest antibacterial effect against <em>Staphylococcus aureus</em> and <em>Heamophilus influenzae</em> with respective minimum inhibitory concentrations of 0.625 and 1.25 μg/cm³. Calculated therapeutic index (&gt;21.05) suggests relative inhalation safety of this compound. Using a gas chromatography/mass spectrometry analysis, E-cinnmaldehyde, benzaldehyde, and eucalyptol were identified as major components of <em>N. cassia</em> bark oil, while leaf oil of <em>C. oppositifolia</em> consisted predominantly of thunbergene, β-caryophyllene, and <em>m</em>-camphorene. Moreover, chemical profile of <em>N. cassia</em> EO in the vapour phase showed E-cinnamaldehyde, <em>o</em>-cymene, and α-pinene as the most abundant volatiles. The results suggest 8-quinolinol for further research focused on development of inhalation therapies. Additionally, the results demonstrate validity of the microplate disc volatilization method, which allows cost and labour effective high-throughput screening of cytotoxicity and antimicrobial activity of volatiles in vapour phase.</div></div>","PeriodicalId":317,"journal":{"name":"Food and Chemical Toxicology","volume":"206 ","pages":"Article 115744"},"PeriodicalIF":3.5,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential mechanism of methamphetamine-induced mitochondrial dysfunction and cell degeneration via direct permeation across mitochondrial membranes: The study on molecular dynamics simulations and in vitro model","authors":"Tanthai Polvat ,&nbsp;Wasinee Khuntawee ,&nbsp;Tanya Prasertporn ,&nbsp;Kornkanok Promthep ,&nbsp;Nathanon Kerdkaen ,&nbsp;Jiraporn Panmanee ,&nbsp;Pornkamon Nalakarn ,&nbsp;Jirasak Wong-ekkabut ,&nbsp;Banthit Chetsawang","doi":"10.1016/j.fct.2025.115743","DOIUrl":"10.1016/j.fct.2025.115743","url":null,"abstract":"<div><div>This study aims to investigate the direct permeation of methamphetamine (METH)-induced mitochondrial dysfunction leading to apoptotic cell death. The molecular dynamics (MD) simulations were performed to study METH and dopamine (DA) penetration through phospholipid membranes, which are abundant on the cellular and mitochondrial membranes. The simulation results showed that METH molecules passively diffuse into the membranes, whereas the DA molecule adsorbs onto the lipid bilayer interface. Additionally, the number of H-bond formations and the distribution lifetime of METH were higher than those of DA. Furthermore, the potential of mean force (PMF) profiles for METH and DA translocating through the lipid bilayer were calculated. The result demonstrated that the free energy barrier of METH is small, and the lowest free energy is located inside the bilayer. On the other hand, a significant energy barrier was found at the bilayer center in the PMF profile of DA. The simulation results suggest that METH can passively penetrate through the lipid bilayer. METH-induced mitochondrial dysfunction leading to cell death was observed in both dopamine transporter (DAT)-expressing cells and non-DAT-expressing cells. This finding highlights the direct permeation of METH across the membrane, inducing impairment of mitochondrial function and cell degeneration.</div></div>","PeriodicalId":317,"journal":{"name":"Food and Chemical Toxicology","volume":"206 ","pages":"Article 115743"},"PeriodicalIF":3.5,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive safety assessment of the probiotic Streptococcus salivarius OSTIA SALI-10","authors":"Cara Marks ,&nbsp;Morvarid Oveisi ,&nbsp;Yan Hei Kelly Choi ,&nbsp;Michael Glogauer ,&nbsp;Abdelahhad Barbour","doi":"10.1016/j.fct.2025.115741","DOIUrl":"10.1016/j.fct.2025.115741","url":null,"abstract":"<div><div>Rigid safety protocols are essential for the development of safe therapeutic agents, including probiotics. With the growing interest in probiotic use for oral health, it is crucial that these beneficial microorganisms adhere to stringent safety assessments, particularly concerning antibiotic resistance. In this study, we characterize <em>Streptococcus salivarius</em> OSTIA SALI-10, a novel strain isolated from the oral cavity of a healthy human adult and assess its safety profile through comprehensive genomic and phenotypic analyses. Although <em>S. salivarius</em> is generally considered a pioneer colonizer of the oral cavity and gastrointestinal tract, not all strains are classified as safe due to potential antibiotic resistance, hemolytic activities, or virulence factors. To address this, we developed a screening methodology that targets antibiotic-resistance genes in this species including resistance to macrolides and tetracyclines. SALI-10 demonstrated susceptibility to clinically relevant antibiotics, ensuring compliance with probiotic safety standards. Additional phenotypic characterization, including carbohydrate fermentation, enzyme profiling, hemolytic activity testing, and biogenic amine production, further reinforced its safety profile. Genomic analysis verified the absence of antibiotic resistance genes (ARGs), virulence factors, and mobile genetic elements (MGEs) associated with ARGs.</div></div>","PeriodicalId":317,"journal":{"name":"Food and Chemical Toxicology","volume":"206 ","pages":"Article 115741"},"PeriodicalIF":3.5,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145060448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subacute exposure of 2, 2′, 4, 4′-tetrabromodiphenyl ether induced liver injury by inhibiting mitochondrial autophagy and increasing NLRP3 inflammasome in mice","authors":"Xiyue Cao ,&nbsp;Weijue Liu ,&nbsp;Shuhua Xi ,&nbsp;Yue Wang","doi":"10.1016/j.fct.2025.115738","DOIUrl":"10.1016/j.fct.2025.115738","url":null,"abstract":"<div><div>2′,4,4′-tetrabrominated diphenyl ethers (BDE-47) is one of the most common brominated flame retardants found in environment and has been demonstrated to be associated with a variety of adverse human health effects. It has been proven to generate liver toxicity, but little is known about the potential mechanism following BDE-47 exposure. Here, hepatotoxicity of BDE-47 in mice was investigated by gavage exposure to BDE-47 (12.5–100mg/kg/day) for 4 weeks. The results showed the increasing of ALT and AST in serum and histopathological change of liver in BDE-47 exposure mice. In addition, we observed that the mitochondrial membrane potential (MMP) decreased and ROS increased with the increase of BDE-47 dose. BDE-47 also up-regulated NLRP3 protein levels, and proteins expression of caspase-1, IL-1β, IL-18 and TNF-α inflammatory factors. Furthermore, mitophagy was blocked with Parkin and PINK1 protein expression decrease and P62 increase in liver of BDE-47 exposure mice. However, activation of mitophagy with autophagy activator rapamycin (RAPA) alleviated liver injury induced by BDE-47 in mice via increasing the protein expression of PINK1 and Parkin, and decreasing NLRP3 and inflammatory factors. These results suggest NLRP3 inflammasome activation-mediated mitochondrial and liver damage in BDE-47-exposed mice is due to mitophagy downregulation, and activating mitophagy can alleviate BDE-47-induced inflammatory damage by regulating NLRP3 inflammasome.</div></div>","PeriodicalId":317,"journal":{"name":"Food and Chemical Toxicology","volume":"206 ","pages":"Article 115738"},"PeriodicalIF":3.5,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early-life PFOA exposure-induced male reproductive adverse effects are associated with the activation of the NF-κB signaling pathway: The mitigative role of inulin","authors":"Ya-Qi Chen ,&nbsp;Jin-Jin Zhang ,&nbsp;Xiao-Fan Guo ,&nbsp;Yu-Kui Chen ,&nbsp;Qin-Yao Zhang ,&nbsp;Qi Wang ,&nbsp;Xiao-Li Xie","doi":"10.1016/j.fct.2025.115731","DOIUrl":"10.1016/j.fct.2025.115731","url":null,"abstract":"<div><div>Perfluorooctanoic acid (PFOA) is widely distributed in various environmental media and has adverse health effects. The underlying mechanisms of the effects of PFOA on the developmental toxicity of the male reproductive system are poorly understood. In this study, 8-week-old C57BL/6 J female mice were given Milli-Q water, PFOA (1 mg/kg/day), PFOA and inulin (5 g/kg/day) or inulin by gavage from the first day of pregnancy until weaning to evaluate the effects of PFOA on the reproductive function of male offspring. Early-life PFOA exposure resulted in decreased sperm quality and testosterone levels, impaired testicular structure, suggesting adverse reproductive effects. According to the transcriptomics analysis, the NF-κB pathway was enriched by KEGG enrichment analysis in the testes of the PFOA group. Consistently, the decreased protein expression ratio of p-p65 to p65 (p-p65/p65) suggested disturbance of the NF-κB signaling pathway. Inulin alleviated testicular structure injury resulting from early-life PFOA exposure, increased sperm quality and testosterone, and decreased the protein expression of the p-p65/p65 ratio. Taken together, our results suggested that early-life PFOA exposure might disrupt the integrity of the blood‒testicular barrier in the testis by activating the NF-κB signaling pathway, resulting in adverse male reproductive effects, which were alleviated by inulin intervention.</div></div>","PeriodicalId":317,"journal":{"name":"Food and Chemical Toxicology","volume":"206 ","pages":"Article 115731"},"PeriodicalIF":3.5,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The tastiest is not always the safest: mercury risks of boneless fish consumption compared to other seafood traded in southeastern Brazil","authors":"Alice de Souza Picaluga ,&nbsp;Monizze Vannuci-Silva ,&nbsp;Lucas Rodrigues Tovar ,&nbsp;Petrus Magnus Amaral Galvão ,&nbsp;Hugo Felipe Gonçalves da Silva ,&nbsp;Haydée Andrade Cunha ,&nbsp;Alexandre F. Azevedo ,&nbsp;Olaf Malm ,&nbsp;José Lailson-Brito ,&nbsp;Tatiana Lemos Bisi","doi":"10.1016/j.fct.2025.115729","DOIUrl":"10.1016/j.fct.2025.115729","url":null,"abstract":"<div><div>Seafood plays a crucial role in the food supply, but it is also a source of contaminants, such as mercury. The ubiquity, high toxicity, and biomagnification of mercury justify its monitoring in seafood as a priority for achieving the 2030 food safety goals. This study aimed to determine the total mercury (THg) and methylmercury (MeHg) contamination in eight seafood species commercially available in a metropolitan region of southeastern Brazil, thereby generating the Estimated Daily Intake (EDI) and the Hazard Quotient (HQ). The lowest THg concentrations were found in pangas and mussels (0.0003 ± 0.0003 and 0.0005 ± 0.0002 mg.kg⁻¹ w.w., respectively), while the highest were found in blue sharks (1.27 ± 0.46 mg kg⁻¹, w.w.). The blue shark was the only species with THg concentrations above the limit of 0.5 mg kg⁻¹ for fish set by the WHO and the most toxic form of mercury, MeHg, predominated in 80 % of them. The blue shark HQ ranged from 0.91 to 2.97 and from 2.06 to 6.71 for Hg and MeHg. Therefore, the consumption of this specific group of seafood should be restricted or preferably avoided. Further studies should enhance the information on fish consumption among vulnerable and more exposed populations to obtain more reliable health risk assessments.</div></div>","PeriodicalId":317,"journal":{"name":"Food and Chemical Toxicology","volume":"206 ","pages":"Article 115729"},"PeriodicalIF":3.5,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}