New microplate disc volatilization assay for in vitro evaluation of toxicity and antimicrobial activity of volatiles in vapour phase

IF 3.5 3区医学 Q2 FOOD SCIENCE & TECHNOLOGY

Food and Chemical Toxicology Pub Date : 2025-09-18 DOI:10.1016/j.fct.2025.115744

Marketa Houdkova , Lucie Vistejnova , Julien Antih , Aishwarya Chaure , Iveta Paurova , Mukund Lal Naik , Pavel Novy , Pavel Klein , Manas Kanti Deb , Ladislav Kokoska

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Abstract

In this study, a novel microplate disc volatilization method for in vitro screening of cytotoxic and antibacterial potential of volatile agents in the vapour phase was designed with the aim to assess their therapeutic potential for development of new inhalation therapies. The toxicity and antibacterial activity of plant-derived volatiles (allyl isothiocyanate, 8-quinolinol, thymohydroquinone, thymoquinone) and essential oils (EOs) from Colebrookea oppositifolia leaf and Neolitsea cassia bark were evaluated against human lung fibroblasts MRC-5 and respiratory pathogens. Allyl isothiocyanate and C. oppositifolia EO did not affect the cell viability (IC₅₀ > 160 μg/cm³), on the other hand thymohydroquinone exhibited the highest cytotoxic effect (IC₅₀ = 5.85 μg/cm³) followed by thymoquinone and N. cassia EO (IC₅₀ = 7.95 and 18.58 μg/cm³, respectively). 8-Quinolinol possessed the strongest antibacterial effect against Staphylococcus aureus and Heamophilus influenzae with respective minimum inhibitory concentrations of 0.625 and 1.25 μg/cm³. Calculated therapeutic index (>21.05) suggests relative inhalation safety of this compound. Using a gas chromatography/mass spectrometry analysis, E-cinnmaldehyde, benzaldehyde, and eucalyptol were identified as major components of N. cassia bark oil, while leaf oil of C. oppositifolia consisted predominantly of thunbergene, β-caryophyllene, and m-camphorene. Moreover, chemical profile of N. cassia EO in the vapour phase showed E-cinnamaldehyde, o-cymene, and α-pinene as the most abundant volatiles. The results suggest 8-quinolinol for further research focused on development of inhalation therapies. Additionally, the results demonstrate validity of the microplate disc volatilization method, which allows cost and labour effective high-throughput screening of cytotoxicity and antimicrobial activity of volatiles in vapour phase.

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新型微孔板圆盘挥发法测定挥发物的气相毒性和抑菌活性。

在本研究中，设计了一种新的微孔板圆盘挥发法，用于体外筛选挥发性药物在气相中的细胞毒性和抗菌潜力，目的是评估其治疗潜力，以开发新的吸入疗法。研究了异硫氰酸烯丙酯、8-喹啉醇、百里香对苯二酚、百里香醌等植物源性挥发物和异硫氰酸对苯二酚精油对人肺成纤维细胞MRC-5和呼吸道病原体的毒性和抗菌活性。异硫氰酸烯丙酯和对花草EO对细胞活性无显著影响（IC50 ~ 160 μg/cm3），而百里香对苯二酚的细胞毒性最强（IC50 = 5.85 μg/cm3），其次是百里香醌和桂花EO （IC50分别为7.95和18.58 μg/cm3）。8-喹啉醇对金黄色葡萄球菌和流感嗜血杆菌的抑菌效果最强，最低抑菌浓度分别为0.625和1.25 μg/cm3。计算的治疗指数（> 21.05）表明该化合物的相对吸入安全性。气相色谱/质谱分析表明，桂皮油的主要成分为e -肉桂醛、苯甲醛和桉树醇，而对叶油的主要成分为雷公烯、β-石竹烯和间樟烯。在气相化学谱中，桂皮N. EO的挥发物主要为e -肉桂醛、o-花香烃和α-蒎烯。结果表明，8-喹啉醇的进一步研究重点是开发吸入疗法。此外，结果证明了微孔板圆盘挥发法的有效性，该方法允许成本和劳动力有效的高通量筛选气相挥发物的细胞毒性和抗菌活性。

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来源期刊

Food and Chemical Toxicology 工程技术-毒理学

CiteScore

10.90

自引率

4.70%

发文量

651

审稿时长

31 days

期刊介绍： Food and Chemical Toxicology (FCT), an internationally renowned journal, that publishes original research articles and reviews on toxic effects, in animals and humans, of natural or synthetic chemicals occurring in the human environment with particular emphasis on food, drugs, and chemicals, including agricultural and industrial safety, and consumer product safety. Areas such as safety evaluation of novel foods and ingredients, biotechnologically-derived products, and nanomaterials are included in the scope of the journal. FCT also encourages submission of papers on inter-relationships between nutrition and toxicology and on in vitro techniques, particularly those fostering the 3 Rs. The principal aim of the journal is to publish high impact, scholarly work and to serve as a multidisciplinary forum for research in toxicology. Papers submitted will be judged on the basis of scientific originality and contribution to the field, quality and subject matter. Studies should address at least one of the following: -Adverse physiological/biochemical, or pathological changes induced by specific defined substances -New techniques for assessing potential toxicity, including molecular biology -Mechanisms underlying toxic phenomena -Toxicological examinations of specific chemicals or consumer products, both those showing adverse effects and those demonstrating safety, that meet current standards of scientific acceptability. Authors must clearly and briefly identify what novel toxic effect (s) or toxic mechanism (s) of the chemical are being reported and what their significance is in the abstract. Furthermore, sufficient doses should be included in order to provide information on NOAEL/LOAEL values.