Comprehensive safety assessment of the probiotic Streptococcus salivarius OSTIA SALI-10

IF 3.5 3区医学 Q2 FOOD SCIENCE & TECHNOLOGY

Food and Chemical Toxicology Pub Date : 2025-09-12 DOI:10.1016/j.fct.2025.115741

Cara Marks , Morvarid Oveisi , Yan Hei Kelly Choi , Michael Glogauer , Abdelahhad Barbour

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引用次数: 0

Abstract

Rigid safety protocols are essential for the development of safe therapeutic agents, including probiotics. With the growing interest in probiotic use for oral health, it is crucial that these beneficial microorganisms adhere to stringent safety assessments, particularly concerning antibiotic resistance. In this study, we characterize Streptococcus salivarius OSTIA SALI-10, a novel strain isolated from the oral cavity of a healthy human adult and assess its safety profile through comprehensive genomic and phenotypic analyses. Although S. salivarius is generally considered a pioneer colonizer of the oral cavity and gastrointestinal tract, not all strains are classified as safe due to potential antibiotic resistance, hemolytic activities, or virulence factors. To address this, we developed a screening methodology that targets antibiotic-resistance genes in this species including resistance to macrolides and tetracyclines. SALI-10 demonstrated susceptibility to clinically relevant antibiotics, ensuring compliance with probiotic safety standards. Additional phenotypic characterization, including carbohydrate fermentation, enzyme profiling, hemolytic activity testing, and biogenic amine production, further reinforced its safety profile. Genomic analysis verified the absence of antibiotic resistance genes (ARGs), virulence factors, and mobile genetic elements (MGEs) associated with ARGs.

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益生菌唾液链球菌OSTIA salii -10的综合安全性评价

严格的安全规程对于开发包括益生菌在内的安全治疗剂至关重要。随着人们对益生菌用于口腔健康的兴趣日益浓厚，至关重要的是，这些有益微生物必须遵守严格的安全性评估，特别是在抗生素耐药性方面。在这项研究中，我们鉴定了一种从健康成人口腔中分离出来的新型唾液链球菌OSTIA salii -10，并通过全面的基因组和表型分析评估了其安全性。虽然唾液链球菌通常被认为是口腔和胃肠道的先驱者，但由于潜在的抗生素耐药性、溶血活性或毒力因素，并非所有菌株都被归类为安全菌株。为了解决这个问题，我们开发了一种筛选方法，针对该物种的抗生素抗性基因，包括对大环内酯类和四环素类的抗性。SALI-10对临床相关抗生素敏感，确保符合益生菌安全标准。额外的表型表征，包括碳水化合物发酵、酶谱、溶血活性测试和生物胺生产，进一步加强了其安全性。基因组分析证实了抗生素耐药基因（ARGs）、毒力因子和与ARGs相关的移动遗传元件（MGEs）的缺失。

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来源期刊

Food and Chemical Toxicology 工程技术-毒理学

CiteScore

10.90

自引率

4.70%

发文量

651

审稿时长

31 days

期刊介绍： Food and Chemical Toxicology (FCT), an internationally renowned journal, that publishes original research articles and reviews on toxic effects, in animals and humans, of natural or synthetic chemicals occurring in the human environment with particular emphasis on food, drugs, and chemicals, including agricultural and industrial safety, and consumer product safety. Areas such as safety evaluation of novel foods and ingredients, biotechnologically-derived products, and nanomaterials are included in the scope of the journal. FCT also encourages submission of papers on inter-relationships between nutrition and toxicology and on in vitro techniques, particularly those fostering the 3 Rs. The principal aim of the journal is to publish high impact, scholarly work and to serve as a multidisciplinary forum for research in toxicology. Papers submitted will be judged on the basis of scientific originality and contribution to the field, quality and subject matter. Studies should address at least one of the following: -Adverse physiological/biochemical, or pathological changes induced by specific defined substances -New techniques for assessing potential toxicity, including molecular biology -Mechanisms underlying toxic phenomena -Toxicological examinations of specific chemicals or consumer products, both those showing adverse effects and those demonstrating safety, that meet current standards of scientific acceptability. Authors must clearly and briefly identify what novel toxic effect (s) or toxic mechanism (s) of the chemical are being reported and what their significance is in the abstract. Furthermore, sufficient doses should be included in order to provide information on NOAEL/LOAEL values.