Virology最新文献_第6页

SARS-CoV-2 Nsp13 helicase modulates miR-146a-mediated signaling pathways SARS-CoV-2 Nsp13解旋酶调节mir -146a介导的信号通路

IF 2.8 3区医学

Virology Pub Date : 2025-03-07 DOI: 10.1016/j.virol.2025.110493

Eryn Lundrigan , Spencer Uguccioni , Christine Hum , Nadine Ahmed , John Paul Pezacki

{"title":"SARS-CoV-2 Nsp13 helicase modulates miR-146a-mediated signaling pathways","authors":"Eryn Lundrigan , Spencer Uguccioni , Christine Hum , Nadine Ahmed , John Paul Pezacki","doi":"10.1016/j.virol.2025.110493","DOIUrl":"10.1016/j.virol.2025.110493","url":null,"abstract":"<div><div>Despite the successful development of vaccines and antiviral therapeutics against SARS-CoV-2, its tendency to mutate rapidly has emphasized the need for continued research to better understand this virus’s mechanism of pathogenesis and interactions with host signaling pathways. In this study, we sought to explore how the SARS-CoV-2 non-structural protein 13 (Nsp13) helicase, a highly conserved coronavirus protein that is essential for viral replication, influences host biological and cellular processes. Global transcriptomic analyses of Nsp13-transfected A549 cells identified changes in pathways involved in post-transcriptional gene silencing and translational repression by RNA, such as microRNAs (miRNAs). Upon further bioinformatic analyses, we identified miR-146a-mediated signaling pathways to be of interest as this miRNA has been previously linked to the regulation of host inflammation and innate immune responses. We found that miR-146a was induced in Nsp13-transfected cells and observed a corresponding decrease in the gene expression of two miR-146a targets, TRAF6 and IRAK1, which are important upstream regulators of NF-kB activation and IFN signaling. These results suggest that Nsp13-induced miR-146a signaling cascades, namely NF-kB activation and SMAD4 signaling, may provide valuable insight for the development of novel antiviral therapeutics against COVID-19 variants.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"606 ","pages":"Article 110493"},"PeriodicalIF":2.8,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143594196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The four Ws of viruses: Where, Which, What and Why - A deep dive into viral evolution 病毒的四个w：在哪里，哪个，什么和为什么-深入研究病毒进化

IF 2.8 3区医学

Virology Pub Date : 2025-03-05 DOI: 10.1016/j.virol.2025.110476

Francesco Favaretto , Emilyn E. Matsumura , Inmaculada Ferriol , Walter Chitarra , Luca Nerva

{"title":"The four Ws of viruses: Where, Which, What and Why - A deep dive into viral evolution","authors":"Francesco Favaretto , Emilyn E. Matsumura , Inmaculada Ferriol , Walter Chitarra , Luca Nerva","doi":"10.1016/j.virol.2025.110476","DOIUrl":"10.1016/j.virol.2025.110476","url":null,"abstract":"<div><div>For centuries, humanity has been captivated by evolution, seeking to unravel the origins of life and identify past patterns with future applications. Viruses, despite their obligate parasitic nature, are the most adaptable biological entities, surpassing cellular life in their variability and adaptability. While many theories about viral evolution exist, a consensus on their origins remains elusive. The quasispecies theory, however, has emerged as a leading framework for understanding viral evolution and, indirectly, their variability and adaptability. This theory illuminates how viruses regulate behaviours such as host range and their symbiotic or antagonistic interactions with hosts.</div><div>This review delves into the most substantiated theories of viral evolution, addressing four fundamental questions relevant to virus ecology: Where did viruses originate? What factors drive viral evolution? What determines the virus host range? And why do viruses adopt pathogenic or mutualistic strategies? We will provide a comprehensive and up-to-date analysis that integrates diverse theoretical perspectives with empirical data, providing a holistic view of viral evolution and its implications for viral behaviour.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"606 ","pages":"Article 110476"},"PeriodicalIF":2.8,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143594197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel reassorted swine H3N2 influenza virus demonstrates an undetected human-to-swine spillover in Latin America and highlights zoonotic risks 一种新型重配猪 H3N2 流感病毒表明拉丁美洲存在未被发现的人对猪溢出现象，并凸显了人畜共患风险

IF 2.8 3区医学

Virology Pub Date : 2025-03-05 DOI: 10.1016/j.virol.2025.110483

Andres F. Ospina-Jimenez , Arlen P. Gomez , Maria A. Rincon-Monroy , Daniel R. Perez , Gloria C. Ramirez-Nieto

{"title":"A novel reassorted swine H3N2 influenza virus demonstrates an undetected human-to-swine spillover in Latin America and highlights zoonotic risks","authors":"Andres F. Ospina-Jimenez , Arlen P. Gomez , Maria A. Rincon-Monroy , Daniel R. Perez , Gloria C. Ramirez-Nieto","doi":"10.1016/j.virol.2025.110483","DOIUrl":"10.1016/j.virol.2025.110483","url":null,"abstract":"<div><div>Influenza A virus (FLUAV) affects a wide range of hosts, including humans and animals, posing a threat to public health. In swine, H3N2 subtype is associated with human-to-swine spillovers of seasonal viruses. In Latin America, the molecular and antigenic characteristics of swine FLUAV H3N2, as well as its phylogenetic origin, are poorly understood. Therefore, the objective of this study was to characterize the first swine H3N2 detected in Colombia. The origin and lineage of the virus were estimated through phylogenetic and molecular clock analyses. Antigenic characterization was achieved by comparing the amino acid constitution of the HA with previously reported swine FLUAVs and seasonal vaccine strains using a sequence-based method. In addition to HA and NA, internal genes were also characterized. The results showed that the Colombian H3N2 corresponded to a novel phylogenetic and antigenic swine FLUAV variant that emerged due to an independent reverse zoonotic event, likely occurring in Colombia in the early 2000s. The immunodominant epitope in the virus was predominantly present in antigenic epitope A, which showed the highest amino acid variation. Some mutations that alter the N-Glycosylation of antigenic sites at the HA were detected. Internally, the virus exhibited pandemic configuration. This study provides the first evidence of a novel FLUAV in Colombia and describes its origin, variability, and persistence in geographically restricted populations, highlighting the need for strengthen molecular surveillance of the virus in animal populations.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"606 ","pages":"Article 110483"},"PeriodicalIF":2.8,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143594126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evidence of novel reassortment in clade 2.3.4.4b avian influenza H5N1 viruses, India, 2024 禽流感H5N1病毒2.3.4.4b进化支新重组的证据，印度，2024

IF 2.8 3区医学

Virology Pub Date : 2025-03-05 DOI: 10.1016/j.virol.2025.110482

Shailesh D. Pawar , Sachin S. Keng , Deeksha S. Tare , Anukumar Balakrishnan , Siba S , Jijo Koshy , Varsha Potdar , Veena Vipat , Satish Gaikwad , Dinesh Singh , Jayati Mullick , Naveen Kumar

{"title":"Evidence of novel reassortment in clade 2.3.4.4b avian influenza H5N1 viruses, India, 2024","authors":"Shailesh D. Pawar , Sachin S. Keng , Deeksha S. Tare , Anukumar Balakrishnan , Siba S , Jijo Koshy , Varsha Potdar , Veena Vipat , Satish Gaikwad , Dinesh Singh , Jayati Mullick , Naveen Kumar","doi":"10.1016/j.virol.2025.110482","DOIUrl":"10.1016/j.virol.2025.110482","url":null,"abstract":"<div><div>H5N1 viruses belonging to clade 2.3.4.4b have caused unprecedented outbreaks globally. Outbreaks of H5N1 virus were reported in poultry and wild birds from Kerala (India) in the year 2024. Samples from birds and the environment were collected. Real-time RT-PCR and virus isolation using embryonated chicken eggs were carried out. Eight out of 20 samples were positive for virus isolation. The virus showed avian type receptor specificity using sialidase assay. Full genome sequencing revealed markers associated with high pathogenicity and mammalian adaptation. All the viruses belonged to a single genotype with multiple reassortments, including internal gene segments from an avian influenza (AI) H3N8 virus reported from Kerala. Surveillance among individuals with probable exposure showed absence of human infection. This is the first report of the genetic and virological characterization of clade 2.3.4.4b H5N1 viruses from India, highlighting the need for increased AI surveillance at the human-animal and domestic-wild bird interfaces.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"606 ","pages":"Article 110482"},"PeriodicalIF":2.8,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143594209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adaptation of single molecule real time (SMRT) sequence technology for hepatitis C virus genome sequencing and identification of resistance-associated substitutions 将单分子实时（SMRT）测序技术用于丙型肝炎病毒基因组测序和耐药性相关替代物的鉴定

IF 2.8 3区医学

Virology Pub Date : 2025-03-03 DOI: 10.1016/j.virol.2025.110481

Cui Zhang, Pei Liu, Jian Li, Mengjie Han, Yuqiu Liu, Wenge Xing, Maofeng Qiu

{"title":"Adaptation of single molecule real time (SMRT) sequence technology for hepatitis C virus genome sequencing and identification of resistance-associated substitutions","authors":"Cui Zhang, Pei Liu, Jian Li, Mengjie Han, Yuqiu Liu, Wenge Xing, Maofeng Qiu","doi":"10.1016/j.virol.2025.110481","DOIUrl":"10.1016/j.virol.2025.110481","url":null,"abstract":"<div><h3>Background and objective</h3><div>Hepatitis C virus (HCV) resistance-associated substitutions (RASs) impact HCV treatment with direct-acting antivirals (DAAs). Therefore, a sensitive sequencing assay for detecting HCV RASs is crucial. PacBio sequencing, a Single molecule real time (SMRT) platform, is capable of long-fragment sequencing. This study aims to assess the prospects of PacBio sequencing by comparing its accuracy with Sanger sequencing at distinct thresholds and its cost-effectiveness with next-generation sequencing (NGS).</div></div><div><h3>Methods</h3><div>A total of 28 specimens were selected from the HCV RNA-positive individuals in Linzhou, China. Of these specimens, the NS3 to NS5B regions were amplified and sent for PacBio sequencing. Sequence processing was accomplished by lima, pbmm2 and quasitools software, with threshold setting at 1%, 5%, 10% and 15%.</div></div><div><h3>Results</h3><div>High-frequency RASs (S122G in NS3, R30Q in NS5A, and C316N in NS5B), rare RASs (V55A, R155P, and S174AT in NS3; Y448H in NS5B), and low-threshold RASs (L31Q and L31QFHY in NS5A; L159F in NS5B) were identified. It was found that the results of HCV RASs at the 10% threshold of PacBio sequencing are comparable to those of Sanger sequencing. In terms of high-throughput sequencing, the price of PacBio sequencing (571 CNY per specimen) is significantly lower than that of NGS (1000 CNY per specimen).</div></div><div><h3>Conclusions</h3><div>In summary, these findings suggest that the adoption of the 10% threshold in PacBio sequencing for the analysis of HCV RASs is advisable. Moreover, given its relatively lower cost, PacBio sequencing holds great promise as a valuable tool for large-scale population sequencing.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"605 ","pages":"Article 110481"},"PeriodicalIF":2.8,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of an apoptotic lentogenic Newcastle disease virus strain by incorporating the p30 protein of African swine fever virus 结合非洲猪瘟病毒p30蛋白的凋亡透镜原性新城疫病毒毒株的研制。

IF 2.8 3区医学

Virology Pub Date : 2025-03-01 DOI: 10.1016/j.virol.2025.110477

Nilave Ranjan Bora, Rakesh Kumar, Sachin Kumar

{"title":"Development of an apoptotic lentogenic Newcastle disease virus strain by incorporating the p30 protein of African swine fever virus","authors":"Nilave Ranjan Bora, Rakesh Kumar, Sachin Kumar","doi":"10.1016/j.virol.2025.110477","DOIUrl":"10.1016/j.virol.2025.110477","url":null,"abstract":"<div><div>Virotherapy is one of the emerging approaches for cancer treatment. Newcastle disease virus (NDV) is a well-studied avian paramyxovirus commonly isolated from birds. Typically, the virulent strains of NDV are acknowledged for their oncolytic properties. The anti-tumor effects of NDV rely on its capacity to trigger apoptosis in cancer cells and elicit inflammatory immune responses against the tumor. However, the virulent strains pose significant challenges for clinical application. This study investigated the development of an apoptotic lentogenic strain of NDV by incorporating the p30 protein gene of the African swine fever virus (ASFV). Previous studies have indicated that the p30 protein interacts with various cellular proteins, including PARP9 and DAB2, which suggests its potential for direct or indirect influence on apoptotic pathways. Our initial data confirmed the upregulation of caspase 3/9, PARP, and cytochrome <em>c</em>, suggesting the pro-apoptotic nature of the p30 protein. Further, a recombinant NDV (rNDV) expressing p30 protein (rNDV-p30) was developed, and its effects were evaluated on human breast cancer (MCF-7) cells. While rNDV alone can't show apoptosis, its variant, rNDV-p30 showed promising apoptotic features in MCF-7 cells. Overall, the results demonstrated the development of rNDV-p30 as an apoptotic virus that offered a novel virotherapy strategy for cancer treatment. Additional research is needed to investigate the underlying mechanisms, safety, and efficacy of the apoptotic activity of the rNDV-p30 and to evaluate the effectiveness of this approach in animal models.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"606 ","pages":"Article 110477"},"PeriodicalIF":2.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143607646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toward a better understanding of genetic diversity and more effective control strategies of major swine viral diseases 为了更好地了解遗传多样性和更有效的控制策略，主要的猪病毒性疾病。

IF 2.8 3区医学

Virology Pub Date : 2025-03-01 DOI: 10.1016/j.virol.2025.110480

Weihuan Fang, Dongwan Yoo, Wentao Li, Hiep Vu

引用次数: 0

Molecular antiviral responses, immune priming and inheritance in insects 昆虫的分子抗病毒反应、免疫启动和遗传

IF 2.8 3区医学

Virology Pub Date : 2025-02-27 DOI: 10.1016/j.virol.2025.110468

Everardo Gutiérrez-Millán, Eduardo Daniel Rodríguez-Aguilar, Mario Henry Rodríguez

{"title":"Molecular antiviral responses, immune priming and inheritance in insects","authors":"Everardo Gutiérrez-Millán, Eduardo Daniel Rodríguez-Aguilar, Mario Henry Rodríguez","doi":"10.1016/j.virol.2025.110468","DOIUrl":"10.1016/j.virol.2025.110468","url":null,"abstract":"<div><div>Viral diseases transmitted by insects to plants cause severe agricultural damage and arboviruses transmitted to humans cause severe disease outbreaks. The interaction between viruses and the insect defences is complex and has evolved into acting-counteracting molecular interplays. Viruses depict complex molecular mechanisms to ensure invasion, replication and exit the insect host cell, to invade other cells. On the other hand, insect cells use molecular strategies to recognize, halt replication and eliminate the invaders. In turn, virus counteract with evasive strategies. The main antiviral defence mechanism RNA interference (RNAi) recognizes and degrades viral RNA, thereby inhibiting viral replication. These in conjunction with other canonical immune pathways, Toll, IMD, JAK/STAT and Akt-ERK developed mainly to combat bacteria, fungi and protozoa, along with mechanisms to eliminate infected cells like apoptosis and phagocytosis comprise a multifactorial system. Insects exposed to an attenuated or sublethal viral infection could respond with faster and enhanced immune responses to the same pathogen (priming), which is like immunological memory in vertebrates. Several mechanisms have been proposed to explain priming, including endoreplication, epigenetic gene modifications by DNA methylation and histone acetylation. Priming could be inherited by the offspring (transgenerational immune priming, TGIP). However, the precise molecular mechanisms underlying TGIP remain to be elucidated. This article reviews the molecular mechanisms employed by insects to combat viral infections, discusses the current information and the outstanding research questions in the area.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"605 ","pages":"Article 110468"},"PeriodicalIF":2.8,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143549778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of reverse genetics system to Chikungunya virus study 在基孔肯雅病毒研究中应用反向遗传学系统

IF 2.8 3区医学

Virology Pub Date : 2025-02-27 DOI: 10.1016/j.virol.2025.110465

Youichi Suzuki

引用次数: 0

Advancing the frontiers of virology in Africa: The African Virologists Network's Mission and Progress 推进非洲病毒学前沿：非洲病毒学家网络的使命和进展。

IF 2.8 3区医学

Virology Pub Date : 2025-02-27 DOI: 10.1016/j.virol.2025.110469

Onoja Anyebe Bernard , Sabastine Eugene Arthur , Maryam Aminu , Evelyn Yayra Bonney , Abdul-Azeez A. Anjorin , Gilbert Nchongboh Chofong , Anna Kafintu-kwashie , Claude Taurai Sabeta , Abdelaziz A. Yassin , Theophilus Korku Adiku

引用次数: 0