Virology最新文献_第6页

Proliferation of Trionyx sinensis hemorrhagic syndrome virus using primary alveolar macrophages in vitro 体外培养原代肺泡巨噬细胞增殖中华Trionyx出血热综合征病毒

IF 2.8 3区医学

Virology Pub Date : 2025-03-28 DOI: 10.1016/j.virol.2025.110522

Xiaoling Dai , Qi Guo , Sunjian Lyu , Weifeng Shen , Li Liu , XiaoJun Xu , Mingxing Zhang , Zhihui Shen , Wanli Shen , Julin Yuan

{"title":"Proliferation of Trionyx sinensis hemorrhagic syndrome virus using primary alveolar macrophages in vitro","authors":"Xiaoling Dai , Qi Guo , Sunjian Lyu , Weifeng Shen , Li Liu , XiaoJun Xu , Mingxing Zhang , Zhihui Shen , Wanli Shen , Julin Yuan","doi":"10.1016/j.virol.2025.110522","DOIUrl":"10.1016/j.virol.2025.110522","url":null,"abstract":"<div><div><em>Trionyx Sinensis</em> (Chinese soft-shelled turtle) parotitis manifests as bacterial or viral infections. Viral parotitis often leads to rapid mortality, posing a significant threat to the <em>T. sinensis</em> aquaculture industry. The causative agent of viral parotitis is <em>T. sinensis</em> Hemorrhagic Syndrome Virus (TSHSV). However, due to the lack of sensitive cells, conducting comprehensive studies on this virus is challenging. In this study, primary macrophages were isolated from the lung tissue of juvenile <em>T. sinensis</em> to establish a method for TSHSV proliferation <em>in vitro</em>. Macrophages were isolated using irrigation method. These cells were cultured in M199 medium supplemented with 25 % fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin, and incubated at 30 °C in a 5 % CO<sub>2</sub> incubator. Macrophages were initially distinguished through morphological observation and an acidic phosphatase assay. In this study, the changes were observed in macrophages infected with TSHSV, and then RT-qPCR, immunofluorescence, and immunohistochemistry techniques were used to evaluate the sensitivity of macrophages for TSHSV. The results demonstrated that the cells washed out from the lung were predominantly identified as alveolar macrophages. After infection with TSHSV, cytopathic effects were observed. Viral nucleic acid segments could be detected in the infected cells, and the expression of antiviral immune-related genes <em>Mx1</em>, <em>OASL</em>, and <em>RSAD2</em> was significantly upregulated. Moreover, a large amount of viral protein was expressed within the infected macrophages, indicating viral replication. Our results suggested that the developed approach for isolating and culturing primary alveolar macrophages of turtles can be employed for <em>in vitro</em> cultivation of TSHSV.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"607 ","pages":"Article 110522"},"PeriodicalIF":2.8,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Infections, genetics, and Alzheimer's disease: Exploring the pathogenic factors for innovative therapies 感染、遗传和阿尔茨海默病：探索创新疗法的致病因素

IF 2.8 3区医学

Virology Pub Date : 2025-03-27 DOI: 10.1016/j.virol.2025.110523

Ramesh Kordi , Ted J. Andrews , Mark D. Hicar

{"title":"Infections, genetics, and Alzheimer's disease: Exploring the pathogenic factors for innovative therapies","authors":"Ramesh Kordi , Ted J. Andrews , Mark D. Hicar","doi":"10.1016/j.virol.2025.110523","DOIUrl":"10.1016/j.virol.2025.110523","url":null,"abstract":"<div><div>Alzheimer's disease (AD) is a progressive neurodegenerative condition that creates a significant global health challenge and profoundly affects patients and their families. Recent research has highlighted the critical role of microorganisms, particularly viral infections, in the pathogenesis of AD.</div><div>The involvement of viral infections in AD pathogenesis is predominantly attributed to their ability to induce neuroinflammation and amyloid beta (Aβ) deposition in the brain. The extant research exploring the relationship between viruses and AD has focused largely on Herpesviridae family. Traces of Herpesviruses, such as Herpes Simplex Virus-1 and Epstein Barr Virus, have been found in the brains of patients with AD. These viruses are thought to contribute to the disease progression by triggering chronic inflammatory responses in the brain. They can remain dormant in the brain, and become reactivated due to stress, a secondary viral infection, or immune-senescence in older adults.</div><div>This review focuses on the association between Herpesviridae and bacterial infections with AD. We explore the genetic factors that might regulate viral illness and discuss clinical trials investigating antiviral and anti-inflammatory agents as possible therapeutic strategies to mitigate cognitive decline in patients with AD. In summary, understanding the interplay between infections, genetic factors, and AD pathogenesis may pave the way for novel therapeutic approaches, facilitating better management and possibly even prevent this debilitating disease.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"607 ","pages":"Article 110523"},"PeriodicalIF":2.8,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure of the turnip yellows virus particles 萝卜黄病毒颗粒的结构

IF 2.8 3区医学

Virology Pub Date : 2025-03-26 DOI: 10.1016/j.virol.2025.110514

Joséphine Lai-Kee-Him , Stefano Trapani , Sylvaine Boissinot , Catherine Reinbold , Chloé Fallet , Aurélie Ancelin , François Lecorre , François Hoh , Véronique Ziegler-Graff , Véronique Brault , Patrick Bron

{"title":"Structure of the turnip yellows virus particles","authors":"Joséphine Lai-Kee-Him , Stefano Trapani , Sylvaine Boissinot , Catherine Reinbold , Chloé Fallet , Aurélie Ancelin , François Lecorre , François Hoh , Véronique Ziegler-Graff , Véronique Brault , Patrick Bron","doi":"10.1016/j.virol.2025.110514","DOIUrl":"10.1016/j.virol.2025.110514","url":null,"abstract":"<div><div>Turnip yellows virus (TuYV) is a plant virus infecting important crops such as oilseed rape. TuYV is phloem-restricted and transmitted by aphids. The capsid contains two subunit types: the major capsid protein (CP) and a minor component (RTP∗) which arises from the C-terminal cleavage of a readthrough product (RTP). RTP∗ contains the CP sequence fused with a structured domain, denoted <sup>N</sup>RTD, which is a key determinant of virus transmission. Though both CP and RTP∗ are involved in virus movement and aphid transmission, how RTP∗ is incorporated into the capsid is poorly understood. We present here the structural characterisation, by immunogold labelling and 3D cryo-EM, of the wild-type TuYV and a mutant whose capsid contains the CP only. We show that incorporation of RTP∗ does not impair the capsid structure, and the <sup>N</sup>RTD does not adopt well-defined positions at the capsid surface. The number of incorporated RTP∗s suggests a random insertion.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"607 ","pages":"Article 110514"},"PeriodicalIF":2.8,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MyosinVb tail inhibits transport of Marburg virus glycoprotein GP to VP40-enriched sites at the plasma membrane MyosinVb尾抑制马尔堡病毒糖蛋白GP向质膜上vp40富集位点的转运

IF 2.8 3区医学

Virology Pub Date : 2025-03-25 DOI: 10.1016/j.virol.2025.110503

Sandro Halwe , Martin Schauflinger , Yuki Takamatsu , Olga Dolnik , Stephan Becker

{"title":"MyosinVb tail inhibits transport of Marburg virus glycoprotein GP to VP40-enriched sites at the plasma membrane","authors":"Sandro Halwe , Martin Schauflinger , Yuki Takamatsu , Olga Dolnik , Stephan Becker","doi":"10.1016/j.virol.2025.110503","DOIUrl":"10.1016/j.virol.2025.110503","url":null,"abstract":"<div><div>Marburg virus (MARV) is the causative agent of severe fever with case fatality rates between 25 and 90 %. The glycoprotein GP is the only surface protein of MARV responsible for receptor recognition and fusion. Therefore, proper intracellular transport of GP to the plasma membrane and incorporation into virus particles is essential for the viral infection cycle. However, neither the exact post-Golgi trafficking route nor the host factors are known that support the transport of GP to the cell surface.</div><div>Using quantitative confocal microscopy and correlative light and electron microscopy (CLEM), we show here that GP colocalized in both transiently transfected and MARV-infected cells with a dominant negative (DN) tail mutant of myosin Vb (MyoVbT), which inhibits trafficking through recycling endosomes. Overexpression of MyoVbT resulted in an aberrant distribution of GP that accumulated in or near perinuclear MyoVbT-containing structures. Simultaneously, we observed significantly reduced GP levels at the plasma membrane and especially at the viral budding sites characterized by clusters of the viral matrix protein VP40. Further, incorporation of GP into VP40-induced filamentous virus-like particles was impaired by MyoVbT. Overall, our results show that intracellular transport of MARV GP is disrupted by a DN mutant of the recycling endosome-associated motor protein MyoVb. These results might indicate a possible role for the endosomal recycling system in MARV GP trafficking to VP40-enriched budding-sites at the plasma membrane.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"607 ","pages":"Article 110503"},"PeriodicalIF":2.8,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143737821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of detoxifying enzymes expression and restriction of picorna-like virus infection by natural polysaccharide extracts in Drosophila cells 天然多糖提取物对果蝇细胞解毒酶表达的调控及对小核糖核酸样病毒感染的抑制作用

IF 2.8 3区医学

Virology Pub Date : 2025-03-24 DOI: 10.1016/j.virol.2025.110513

Gabrielle Haas , Mélodie Seiler , Jenny Nguyen , Laurent Troxler , Samuel Pennarun , Elise Lefebvre , Yasmine Benamrouche , Loriane Loizeau , Cody Reinbolt , Ming Liang , Xiaoliang Lin , Wenzhi Li , Zumeng Xia , Joao T. Marques , Jean-Luc Imler

{"title":"Regulation of detoxifying enzymes expression and restriction of picorna-like virus infection by natural polysaccharide extracts in Drosophila cells","authors":"Gabrielle Haas , Mélodie Seiler , Jenny Nguyen , Laurent Troxler , Samuel Pennarun , Elise Lefebvre , Yasmine Benamrouche , Loriane Loizeau , Cody Reinbolt , Ming Liang , Xiaoliang Lin , Wenzhi Li , Zumeng Xia , Joao T. Marques , Jean-Luc Imler","doi":"10.1016/j.virol.2025.110513","DOIUrl":"10.1016/j.virol.2025.110513","url":null,"abstract":"<div><div>The world is currently witnessing a rise in viral infections, while the availability of antiviral drugs remains limited. Traditional Chinese medicine (TCM) has historically served as a valuable source of novel compounds for disease treatment. In this study, we assessed the antiviral potential of various TCM compounds using <em>Drosophila melanogaster</em> as a model organism. Our findings reveal that natural polysaccharide extracts, prepared from 10 commonly used medicinal herbs or fungi, exhibit antiviral activity against two picorna-like viruses. Importantly, the antiviral effect is not directly attributable to the compound itself but is instead mediated by cellular responses induced by treatment with the extract. We observed that the polysaccharide extract triggers a broad transcriptional response, which partially overlaps with NF-κB pathway activation in <em>Drosophila</em>. However, the antiviral activity of the extract was independent of classical innate immune pathways, such as RNA interference or NF-κB signaling. Instead, the extract appears to uniquely stimulate detoxification pathways, including upregulation of cytochrome P450 and glutathione S-transferase genes, which correlates with its antiviral effects.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"607 ","pages":"Article 110513"},"PeriodicalIF":2.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiple exposures to SARS-CoV-2 Spike enhance cross-reactive antibody-dependent cellular cytotoxicity against SARS-CoV-1 多次暴露于SARS-CoV-2刺突增强对SARS-CoV-1的交叉反应性抗体依赖性细胞毒性

IF 2.8 3区医学

Virology Pub Date : 2025-03-22 DOI: 10.1016/j.virol.2025.110512

Guillaume Beaudoin-Bussières , Alexandra Tauzin , Katrina Dionne , Omar El Ferri , Mehdi Benlarbi , Catherine Bourassa , Halima Medjahed , Renée Bazin , Marceline Côté , Andrés Finzi

{"title":"Multiple exposures to SARS-CoV-2 Spike enhance cross-reactive antibody-dependent cellular cytotoxicity against SARS-CoV-1","authors":"Guillaume Beaudoin-Bussières , Alexandra Tauzin , Katrina Dionne , Omar El Ferri , Mehdi Benlarbi , Catherine Bourassa , Halima Medjahed , Renée Bazin , Marceline Côté , Andrés Finzi","doi":"10.1016/j.virol.2025.110512","DOIUrl":"10.1016/j.virol.2025.110512","url":null,"abstract":"<div><div>Vaccination or infection by SARS-CoV-2 elicits a protective immune response against severe outcomes. It has been reported that SARS-CoV-2 infection or vaccination elicits cross-reactive antibodies against other betacoronaviruses. While plasma neutralizing capacity was studied in great detail, their Fc-effector functions remain understudied. Here, we analyzed Spike recognition, neutralization and antibody-dependent cellular cytotoxicity (ADCC) against D614G, a recent Omicron subvariant of SARS-CoV-2 (JN.1) and SARS-CoV-1. Plasma from individuals before their first dose of mRNA vaccine, and following their second, third and sixth doses were analyzed. Despite poor neutralization activity observed after the second and third vaccine doses, ADCC was readily detected. By the sixth dose, individuals could neutralize and mediate ADCC against JN.1 and SARS-CoV-1. Since previous reports have shown that Fc-effector functions were associated with survival from acute infection, these results suggest that ADCC could help in combating future SARS-CoV-2 variants as well as closely related coronaviruses.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"607 ","pages":"Article 110512"},"PeriodicalIF":2.8,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143704386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Translational research on pandemic virus infection using nonhuman primate models 利用非人类灵长类动物模型对大流行性病毒感染的转化研究

IF 2.8 3区医学

Virology Pub Date : 2025-03-22 DOI: 10.1016/j.virol.2025.110511

Hirohito Ishigaki , Yasushi Itoh

{"title":"Translational research on pandemic virus infection using nonhuman primate models","authors":"Hirohito Ishigaki , Yasushi Itoh","doi":"10.1016/j.virol.2025.110511","DOIUrl":"10.1016/j.virol.2025.110511","url":null,"abstract":"<div><div>After the COVID-19 pandemic, nonhuman primate (NHP) models, which are necessary for the rapid development of vaccines and new medical therapies, have become important in studies on infectious diseases because of their genetic, metabolic, and immunological similarities to humans. Our group has long been using NHP models in studies on infectious diseases including H1N1 influenza pandemic and COVID-19. Despite limitations such as the limited number of animals and the husbandry requirements, NHP models have contributed to the prediction of the pathogenicity of emerging viruses and the evaluation of the efficacy of vaccines and therapeutics due to the similarity of NHP models to humans before starting clinical trials to select good candidates of vaccines and drugs. In this review, the findings obtained in NHP infectious disease models of influenza and COVID-19 are summarized to clarify the benefits of NHP models for studies on infectious diseases. We believe that this review will support future research in exploring new perspectives for the development of vaccines and therapies targeting influenza, COVID-19, and infectious diseases in future pandemics.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"606 ","pages":"Article 110511"},"PeriodicalIF":2.8,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143687770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Ethyl caffeate as a novel targeted inhibitor of 3CLpro with antiviral activity against porcine epidemic diarrhea virus” [Virology 604 (2025) 110406] 咖啡酸乙酯作为一种新型 3CLpro 靶向抑制剂，对猪流行性腹泻病毒具有抗病毒活性》[病毒学 604 (2025) 110406] 勘误。

IF 2.8 3区医学

Virology Pub Date : 2025-03-22 DOI: 10.1016/j.virol.2025.110497

Limin Jiang, Minghui Gu, Jiawei Xiao, Yingying Zhao, Fanbo Shen, Xingyang Guo, Hansong Li, Donghua Guo, Chunqiu Li, Qinghe Zhu, Dan Yang, Xiaoxu Xing, Dongbo Sun

引用次数: 0

Defining diverse spike-receptor interactions involved in SARS-CoV-2 entry: Mechanisms and therapeutic opportunities 定义SARS-CoV-2进入过程中涉及的多种刺突受体相互作用：机制和治疗机会

IF 2.8 3区医学

Virology Pub Date : 2025-03-21 DOI: 10.1016/j.virol.2025.110507

Michael Anderson, Julian Lopez, Maya Wyr, Peter W. Ramirez

引用次数: 0

An 11-year retrospective study on hepatitis C in Saudi Arabia: Seroconversion, recovery rates, and viral genotype distribution 沙特阿拉伯一项为期11年的丙型肝炎回顾性研究：血清转化、恢复率和病毒基因型分布

IF 2.8 3区医学

Virology Pub Date : 2025-03-21 DOI: 10.1016/j.virol.2025.110505

Adnan A. Mubaraki , Mohammed A. Alabdalli , Ahmed K. Shawush , Muhanna A. Alhusayni , Abdullah A. Hammadi , Awatief A. Edries , Daifallah Alaboud , Ahmed S. Abdel-Moneim

{"title":"An 11-year retrospective study on hepatitis C in Saudi Arabia: Seroconversion, recovery rates, and viral genotype distribution","authors":"Adnan A. Mubaraki , Mohammed A. Alabdalli , Ahmed K. Shawush , Muhanna A. Alhusayni , Abdullah A. Hammadi , Awatief A. Edries , Daifallah Alaboud , Ahmed S. Abdel-Moneim","doi":"10.1016/j.virol.2025.110505","DOIUrl":"10.1016/j.virol.2025.110505","url":null,"abstract":"<div><div>Hepatitis C virus (HCV) infection remains a global health concern. This study analyzed 95,864 plasma samples from Saudi patients between 2011 and 2022 to examine HCV seroconversion, viral load, and genotype distribution. Serological screening was performed using the ARCHITECT anti-HCV assay, and HCV RNA levels were quantified with real-time RT-PCR. Of the 970 HCV-positive cases, 47.9 % experienced spontaneous recovery, while 52.1 % had persistent infection. The annual seropositivity rate declined significantly from 2.05 % in 2011 to 0.34 % in 2022. Genotyping of 107 persistently infected samples showed genotypes 4 (49.5 %) and 1a (17.8 %) as the most common, with other genotypes appearing less frequently. Additionally, 13 (12.1 %) samples had untypable genotypes. This study highlights the decrease in HCV infection rates, the high rate of spontaneous recovery, and the predominance of genotypes 4 and 1a. Ongoing surveillance and genotyping, including untypable cases, are essential for effective HCV management in Saudi Arabia.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"607 ","pages":"Article 110505"},"PeriodicalIF":2.8,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143737822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0