萝卜黄病毒颗粒的结构

IF 2.8 3区医学 Q3 VIROLOGY

Virology Pub Date : 2025-03-26 DOI:10.1016/j.virol.2025.110514

Joséphine Lai-Kee-Him , Stefano Trapani , Sylvaine Boissinot , Catherine Reinbold , Chloé Fallet , Aurélie Ancelin , François Lecorre , François Hoh , Véronique Ziegler-Graff , Véronique Brault , Patrick Bron

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引用次数: 0

摘要

芜菁黄病毒（TuYV）是一种侵染油菜等重要作物的植物病毒。TuYV受韧皮部限制，由蚜虫传播。衣壳包含两种亚基类型：主要衣壳蛋白（CP）和次要组分（RTP *），其产生于读透产物（RTP）的c端裂解。RTP *包含CP序列与一个结构域融合，称为NRTD，这是病毒传播的关键决定因素。虽然CP和RTP *都参与了病毒的运动和蚜虫的传播，但RTP *是如何被纳入衣壳的还不清楚。通过免疫金标记和3D冷冻电镜，我们在这里展示了野生型TuYV和衣壳仅含有CP的突变体的结构特征。我们发现RTP *的掺入不会破坏衣壳结构，NRTD也不会在衣壳表面形成明确的位置。合并RTP *的数目表明是随机插入的。

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Structure of the turnip yellows virus particles

Turnip yellows virus (TuYV) is a plant virus infecting important crops such as oilseed rape. TuYV is phloem-restricted and transmitted by aphids. The capsid contains two subunit types: the major capsid protein (CP) and a minor component (RTP∗) which arises from the C-terminal cleavage of a readthrough product (RTP). RTP∗ contains the CP sequence fused with a structured domain, denoted ^NRTD, which is a key determinant of virus transmission. Though both CP and RTP∗ are involved in virus movement and aphid transmission, how RTP∗ is incorporated into the capsid is poorly understood. We present here the structural characterisation, by immunogold labelling and 3D cryo-EM, of the wild-type TuYV and a mutant whose capsid contains the CP only. We show that incorporation of RTP∗ does not impair the capsid structure, and the ^NRTD does not adopt well-defined positions at the capsid surface. The number of incorporated RTP∗s suggests a random insertion.

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来源期刊

Virology 医学-病毒学

CiteScore

6.00

自引率

0.00%

发文量

157

审稿时长

50 days

期刊介绍： Launched in 1955, Virology is a broad and inclusive journal that welcomes submissions on all aspects of virology including plant, animal, microbial and human viruses. The journal publishes basic research as well as pre-clinical and clinical studies of vaccines, anti-viral drugs and their development, anti-viral therapies, and computational studies of virus infections. Any submission that is of broad interest to the community of virologists/vaccinologists and reporting scientifically accurate and valuable research will be considered for publication, including negative findings and multidisciplinary work.Virology is open to reviews, research manuscripts, short communication, registered reports as well as follow-up manuscripts.