Virology最新文献

Revisiting the roles of trypsin in the productive infection of porcine deltacoronavirus in porcine-derived cells

IF 2.8 3区医学

Virology Pub Date : 2025-02-14 DOI: 10.1016/j.virol.2025.110453

Wenwen Xiao , Zhuang Li , Chaoqun Chen , Yuting Shi , Puxian Fang , Shaobo Xiao , Liurong Fang

{"title":"Revisiting the roles of trypsin in the productive infection of porcine deltacoronavirus in porcine-derived cells","authors":"Wenwen Xiao , Zhuang Li , Chaoqun Chen , Yuting Shi , Puxian Fang , Shaobo Xiao , Liurong Fang","doi":"10.1016/j.virol.2025.110453","DOIUrl":"10.1016/j.virol.2025.110453","url":null,"abstract":"<div><div>Porcine deltacoronavirus (PDCoV) is an emerging enteric coronavirus with the potential for interspecies transmission. Trypsin has been shown to play a positive role in the isolation and multiplication of PDCoV in vitro, however, the functions of trypsin during PDCoV replication cycle remain controversial. In this study, we revisited the roles of trypsin for PDCoV infection by utilizing two kinds of PDCoV, PDCoV<sup>T+</sup> and PDCoV<sup>T−</sup>, which were prepared in the presence or absence of trypsin, respectively. We found that PDCoV<sup>T+</sup> was able to continuously proliferate in the medium containing trypsin, achieving a higher titer as the infection progress in LLC-PK1 and other tested porcine-derived cells. However, its replication was only transiently improved at 12 hours post-infection, and lower viral titers were observed under trypsin-free culture conditions. Furthermore, the trypsin-mediated enhancement of viral replication could be inhibited by trypsin inhibitor SBTI, suggesting that the second-round viral reproduction of PDCoV<sup>T</sup><sup>+</sup> might be impeded without trypsin. We further investigated the replication dynamics of PDCoV<sup>T−</sup> in LLC-PK1 cells in the presence or absence of trypsin. The results indicated that PDCoV<sup>T−</sup> generated lower viral titers under trypsin-free culture conditions, while the addition of trypsin reverted the infectivity of PDCoV<sup>T−</sup>. Additionally, we demonstrated that trypsin cleaved the PDCoV spike protein, activating viral attachment and internalization. Moreover, trypsin promoted viral replication and release, accelerating PDCoV maturation and facilitating second-round infection. Taken together, this study systematically revaluated and emphasized an essential role of trypsin in PDCoV infection, providing mechanistic insights into the productive infection of PDCoV in porcine-derived cells.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"604 ","pages":"Article 110453"},"PeriodicalIF":2.8,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete genomic analysis of tomato chlorosis virus isolates from Sichuan and Chongqing, China

IF 2.8 3区医学

Virology Pub Date : 2025-02-11 DOI: 10.1016/j.virol.2025.110447

Xiaolong Yang, Jie Li, Mingrui Gao, Puxin Huang, Mingjun Li, Gentu Wu, Ling Qing

{"title":"Complete genomic analysis of tomato chlorosis virus isolates from Sichuan and Chongqing, China","authors":"Xiaolong Yang, Jie Li, Mingrui Gao, Puxin Huang, Mingjun Li, Gentu Wu, Ling Qing","doi":"10.1016/j.virol.2025.110447","DOIUrl":"10.1016/j.virol.2025.110447","url":null,"abstract":"<div><div>Tomato chlorosis virus (ToCV) has recently emerged as a significant threat to crop production in China. In this study, we collected tomato leaf samples displaying symptoms of interveinal chlorosis, curling, and yellowing from Sichuan Province and Chongqing City. Reverse transcription polymerase chain reaction (RT-PCR) analysis confirmed that 3 out of 10 samples from Sichuan and 9 out of 24 samples from Chongqing were infected with ToCV. Complete genomic sequences of ToCV were assembled from the cDNA of two infected leaves (SC744 and CQ630), which were deposited in GenBank under accession numbers CQ630: PQ153240 and PQ153241 and SC744: PQ261056 and PQ261057. Phylogenetic analysis indicated that ToCV isolates from the Sichuan Province and Chongqing City were closely related to the ToCV-BJ isolate. The sequence identity matrix shows that p22 has the highest variability at 94.3%–98.4%. p6 is the most conserved, with identities between 98% and 100%. In RNA2, p4 varies from 93.9% to 100%, while Hsp70 remains highly conserved at 97.9%–99%. This study provides the first report on the complete genomic sequences of ToCV from Sichuan Province and Chongqing City, enhancing our understanding of ToCV distribution and genetic diversity in China.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"604 ","pages":"Article 110447"},"PeriodicalIF":2.8,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revolutionizing viral resistance strategies in rice: Evolution from RNAi to precision genome editing

IF 2.8 3区医学

Virology Pub Date : 2025-02-10 DOI: 10.1016/j.virol.2025.110449

Gaurav Kumar, Indranil Dasgupta

引用次数: 0

ATF6-mediated mild ER stress inhibits HBV transcription and replication, which is dependent on mTOR activation

IF 2.8 3区医学

Virology Pub Date : 2025-02-06 DOI: 10.1016/j.virol.2025.110448

Lin Lu , Ying Feng , Yucai Geng , Zhixiang Liu , Yan Wu , Chen Cai , Ji Zhang , Xingda Huang , Tongchun Xue , Bo Gao

{"title":"ATF6-mediated mild ER stress inhibits HBV transcription and replication, which is dependent on mTOR activation","authors":"Lin Lu , Ying Feng , Yucai Geng , Zhixiang Liu , Yan Wu , Chen Cai , Ji Zhang , Xingda Huang , Tongchun Xue , Bo Gao","doi":"10.1016/j.virol.2025.110448","DOIUrl":"10.1016/j.virol.2025.110448","url":null,"abstract":"<div><div>Chronic hepatitis B (CHB) remains a serious global health problem. In our previous investigation, HBV was found to activate a mild ER stress, which facilitated the establishment of persistent HBV infection. However, the role of ER stress manipulation in HBV replication and its underlying mechanisms remain still unclear. Our data showed that mild ER stress inhibited HBV transcription and replication, while severe ER stress enhanced them. Mechanistically, in contrary to the effect on HBV replication, mild ER stress activated whereas severe ER stress inhibited mTOR signaling in HBV-infected cells. Further, mTOR signaling was revealed to be critical for mild ER stress-mediated HBV inhibition. Furthermore, ATF6 but not PERK or IRE1α was found to be involved in mild ER stress-mediated mTOR and the following HBV inhibition. Moreover, ATF6, <em>per se</em>, could inhibit HBV transcription and replication via activating mTOR signaling. Together, ATF6-mediated mild ER stress inhibited HBV transcription and replication through mTOR activation, which might present as an important therapeutic target for CHB patients.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"604 ","pages":"Article 110448"},"PeriodicalIF":2.8,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intraspecific SARS-CoV-2 Delta variant transmission among red fox (Vulpes vulpes) and striped skunk (Mephitis mephitis)

IF 2.8 3区医学

Virology Pub Date : 2025-02-03 DOI: 10.1016/j.virol.2025.110446

Stephanie M. Porter , Airn E. Hartwig , Marissa Quilici , Angela M. Bosco-Lauth , J. Jeffrey Root

引用次数: 0

Human viruses: An ever-increasing list

IF 2.8 3区医学

Virology Pub Date : 2025-02-02 DOI: 10.1016/j.virol.2025.110445

Mei He, Cheng-Qiang He, Nai-Zheng Ding

引用次数: 0

Detection of African swine fever virus antibodies using p11.5 and p14.5 protein-based indirect ELISA 基于p11.5和p14.5蛋白的间接ELISA检测非洲猪瘟病毒抗体。

IF 2.8 3区医学

Virology Pub Date : 2025-02-01 DOI: 10.1016/j.virol.2024.110335

Aiping Wang , Hehe Zhao , Hongliang Liu , Yumei Chen , Jingming Zhou , Xifang Zhu , Chao Liang , Peiyang Ding , Enping Liu , Gaiping Zhang

引用次数: 0

Antibody responses in omicron BF.7-infected patients vaccinated with inactivated SARS-CoV-2 灭活SARS-CoV-2疫苗接种组粒bf .7感染患者的抗体应答

IF 2.8 3区医学

Virology Pub Date : 2025-02-01 DOI: 10.1016/j.virol.2025.110404

Yabo Zhao , Weili Li , Liming Xu , Zhanling Tao , Junhe Zhang

{"title":"Antibody responses in omicron BF.7-infected patients vaccinated with inactivated SARS-CoV-2","authors":"Yabo Zhao , Weili Li , Liming Xu , Zhanling Tao , Junhe Zhang","doi":"10.1016/j.virol.2025.110404","DOIUrl":"10.1016/j.virol.2025.110404","url":null,"abstract":"<div><h3>Objective</h3><div>Our study aimed to investigate antibody responses in omicron BF.7-infected patients after being vaccinated with inactivated SARS-CoV-2.</div></div><div><h3>Methods</h3><div>Blood serum samples were collected every 2–7 d, 1 w before infection, during the acute infection period and recovery period, and every month after recovery to detect IgG, IgM, IgA, neutralizing antibodies, and neutralizing antibodies against different omicrons in the acute phase.</div></div><div><h3>Results</h3><div>The levels of IgG, IgA and neutralizing antibodies increased sharply at 6–7 d after infection, and the levels of IgG and neutralizing antibodies peaked rapidly within 1–2 w, lasting for 3–4 w, and the antibody levels gradually decreased at 4–8 w after infection. The level of neutralizing antibodies against the wild-type strain SARS-CoV-2 was much greater than that against the variant strains during the acute infection period.</div></div><div><h3>Conclusion</h3><div>After being infected with omicron BF.7, the IgG and neutralizing antibodies in inactivated SARS-CoV-2-vaccinated individuals increase sharply 6–7 days after infection, which is earlier than those in the initial WT SARS-CoV-2 infection, and the peak time within 1–2 w is shorter. The levels of IgG, neutralizing antibody, and IgA antibodies are high, and the levels of IgM antibodies are low; however, the neutralizing antibodies are mainly against the wild-type strain of SARS-CoV-2.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"603 ","pages":"Article 110404"},"PeriodicalIF":2.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143019332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The battle between infectious bronchitis virus and innate immunity: A mini review 传染性支气管炎病毒和先天免疫之间的斗争：一个小回顾。

IF 2.8 3区医学

Virology Pub Date : 2025-02-01 DOI: 10.1016/j.virol.2024.110321

Hao Dong , Shengkui Xu , Peng Li , Wenke Ruan

引用次数: 0

Early evolution of mumps-HCV chimeric viruses in Vero cells induces loss of HCV gene expression and promotes accumulation of substitutions uncharacteristic of mumps strains 在Vero细胞中，腮腺炎-丙型肝炎病毒嵌合病毒的早期进化诱导了丙型肝炎病毒基因表达的缺失，并促进了非腮腺炎毒株特征的取代的积累。

IF 2.8 3区医学

Virology Pub Date : 2025-02-01 DOI: 10.1016/j.virol.2024.110379

Dorotea Pali , Dubravko Forčić , Maja Jagušić , Tanja Košutić Gulija , Mirna Jurković , Marko Babić , Daniela Kalafatovic , Jelena Ivančić-Jelečki

引用次数: 0