Virology最新文献_第2页

Diallyl trisulfide inhibits in vitro replication of the Japanese encephalitis virus by modulating autophagy via mTOR-dependent pathway 二烯丙基三硫醚通过mtor依赖途径调节自噬抑制乙型脑炎病毒的体外复制

IF 2.8 3区医学

Virology Pub Date : 2025-05-13 DOI: 10.1016/j.virol.2025.110575

Shivani Marandi , Krishna P. Bhabak , Sachin Kumar

{"title":"Diallyl trisulfide inhibits in vitro replication of the Japanese encephalitis virus by modulating autophagy via mTOR-dependent pathway","authors":"Shivani Marandi , Krishna P. Bhabak , Sachin Kumar","doi":"10.1016/j.virol.2025.110575","DOIUrl":"10.1016/j.virol.2025.110575","url":null,"abstract":"<div><div>Japanese encephalitis is a neurological disease caused by the mosquito-borne Japanese encephalitis virus (JEV). The clinically approved antiviral drugs for JEV infection are not available. In our present study, we investigated the antiviral activity of garlic oil and its key organosulfur compounds against JEV. The garlic oil showed anti-JEV activity in Neuro-2a cells at a 20 μg/ml concentration. Further, the components of garlic oil, i.e., diallyl sulfide (DAS), diallyl disulfide (DADS), and diallyl trisulfide (DATS), were screened for their anti-JEV activity. DATS was active among these compounds and displayed higher antiviral activity against JEV than DAS and DADS. Moreover, DATS inhibited JEV replication in a dose- and time-dependent manner. Mechanistic investigations revealed the activation of mTOR signaling associated protein levels (phospho-mTOR, mTOR, phospho-AKT, AKT) and phospho-p62 autophagy marker in JEV-infected Neuro-2a cells after 48 h post-treatment with DATS. These results demonstrate that DATS inhibits JEV replication by suppressing autophagy via mTOR-dependent pathway.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"610 ","pages":"Article 110575"},"PeriodicalIF":2.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144123448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of watermelon mosaic virus-Beijing isolate infectious clone and study of the dynamic localization and accumulation of virus-encoded proteins 西瓜花叶病毒北京分离株感染克隆的构建及病毒编码蛋白的动态定位和积累研究

IF 2.8 3区医学

Virology Pub Date : 2025-05-13 DOI: 10.1016/j.virol.2025.110571

Mengjiao Pan , Linhao Ge , Weifang Gao , Zhaolei Li , Xue Li , Yalin Chen , Mingxuan Jia , Minghong Lu , Shoulin Jiang , Hongguang Cui , Wenxing Liang , Yaqin Wang , Fangfang Li

{"title":"Construction of watermelon mosaic virus-Beijing isolate infectious clone and study of the dynamic localization and accumulation of virus-encoded proteins","authors":"Mengjiao Pan , Linhao Ge , Weifang Gao , Zhaolei Li , Xue Li , Yalin Chen , Mingxuan Jia , Minghong Lu , Shoulin Jiang , Hongguang Cui , Wenxing Liang , Yaqin Wang , Fangfang Li","doi":"10.1016/j.virol.2025.110571","DOIUrl":"10.1016/j.virol.2025.110571","url":null,"abstract":"<div><div><em>Potyvirus</em> is the largest genus of plant RNA viruses, and the members in the genus are known to cause significant damage to a wide variety of crops. In this study, we performed small RNA (sRNA) deep sequencing for identification of potential virus (es) in collected cucumber leaves with mosaic symptoms from fields in Beijing. Through this high-throughput sequencing and subsequent PCR amplification, we obtained a complete viral genome sequence of 10,044 bp, which showed the highest similarity to the watermelon mosaic virus (WMV) isolate FBR04-37 and exhibited the typical characteristics of potyviruses in its genome organization. The obtained WMV isolate was designated as WMV-BJ. We then constructed the infectious clone of WMV-BJ, which can successfully infect six kinds of cucurbitaceous plants by agroinfiltration. Among these, <em>Cucumis melo</em> showed obvious symptoms such as shrinkage and mosaic compared to other cucurbitaceous plants infected by this virus infectious clone. To understand the biological function of WMV-BJ, we further analyzed the dynamic subcellular localization and protein accumulation of WMV-BJ encoded 11 viral proteins. The results showed the WMV-BJ-encoded proteins displayed diverse subcellular localizations, and most viral proteins were easily degraded after expression in plant cells. Together, the construction of the WMV-BJ infectious clone in this study provides a valuable tool for further exploring the biology of this virus and the interaction between WMV-BJ and host plant, and useful information for investigating the functions of WMV-encoded proteins.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"609 ","pages":"Article 110571"},"PeriodicalIF":2.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanopore sequencing-based measurement of paramyxovirus RNA editing reveals virus-specific differences in editing efficiency of mRNA, antigenome and genome 基于纳米孔测序的副粘病毒RNA编辑测量揭示了病毒特异性mRNA，抗基因组和基因组编辑效率的差异

IF 2.8 3区医学

Virology Pub Date : 2025-05-12 DOI: 10.1016/j.virol.2025.110572

Yusuke Masuda , Tofazzal Md Rakib , Lipi Akter , Keisuke Nakagawa , Kiyotada Naitou , Akatsuki Saito , Ryoji Yamaguchi , Yusuke Matsumoto

{"title":"Nanopore sequencing-based measurement of paramyxovirus RNA editing reveals virus-specific differences in editing efficiency of mRNA, antigenome and genome","authors":"Yusuke Masuda , Tofazzal Md Rakib , Lipi Akter , Keisuke Nakagawa , Kiyotada Naitou , Akatsuki Saito , Ryoji Yamaguchi , Yusuke Matsumoto","doi":"10.1016/j.virol.2025.110572","DOIUrl":"10.1016/j.virol.2025.110572","url":null,"abstract":"<div><div>Paramyxovirus polymerase recognizes an RNA editing signal on the viral genome and transcribes mRNA in which guanine nucleotides are inserted in a template-independent manner. This enables the synthesis of multiple proteins from a single gene, which is important for viral growth. We developed a method to quantify RNA editing efficiency using Oxford Nanopore Technologies’ MinION platform. We performed sequence analysis of reverse transcription-PCR amplicons with the RNA editing sites in cells infected with Sendai virus (SeV) and canine distemper virus (CDV). By modifying reverse transcription primers, we simultaneously assessed RNA editing efficiency in mRNA, antigenome and genome. We observed distinct differences in mRNA editing efficiency between SeV and CDV. Notably, while RNA editing in SeV is confined to mRNA, in CDV it is also observed in antigenome/genome. (Anti)genomes harboring extra nucleotides may deviate from a multiple-of-six sequence, suggesting that RNAs not following the “Rule of Six” are produced in CDV-infected cells.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"609 ","pages":"Article 110572"},"PeriodicalIF":2.8,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of a novel phage vB_EcoP_P64441 and its potential role in controlling uropathogenic Escherichia coli (UPEC) and biofilms formation 新型噬菌体vB_EcoP_P64441在控制尿路致病性大肠杆菌（UPEC）和生物膜形成中的潜在作用

IF 2.8 3区医学

Virology Pub Date : 2025-05-10 DOI: 10.1016/j.virol.2025.110570

Xiaoyue Li , Sixiang Xu , Libin Tan , Xiaona Yan , Xin Wang , Zhuojun Li , Long Chen , Wei Zhang

{"title":"Characterization of a novel phage vB_EcoP_P64441 and its potential role in controlling uropathogenic Escherichia coli (UPEC) and biofilms formation","authors":"Xiaoyue Li , Sixiang Xu , Libin Tan , Xiaona Yan , Xin Wang , Zhuojun Li , Long Chen , Wei Zhang","doi":"10.1016/j.virol.2025.110570","DOIUrl":"10.1016/j.virol.2025.110570","url":null,"abstract":"<div><div>Urinary tract infections are one of the most common infectious diseases worldwide, with uropathogenic <em>Escherichia coli</em> (UPEC) being the leading cause. Phage therapy has emerged as a promising alternative due to the increasing prevalence of multidrug resistance UPEC strains. In this study, we isolated and characterized a novel phage, vB_EcoP_P64441, which lysed UPEC, including serogroup O75. The phage can survive from 4 to 50 °C and a pH range of 3–11. The optimal MOI was 1, latent period was 4 min, and burst size was 10 PFU/cell. Whole genome sequencing revealed that it consisted of 42,960 base pairs that encoded 53 CDSs, with no lysogenic, toxin, or tRNA-associated genes detected. vB_EcoP_P64441 was classified as a new species within the family <em>Autographiviridae</em>, subfamily <em>Slopekvirinae</em>. The results of the inhibition experiments showed that vB_EcoP_P64441 had a good suppression effect on the growth of planktonic UPEC. Phage either alone or in combination with cefotaxime was effective in inhibiting biofilm formation of UPEC in vitro. Phage strongly inhibited bacterial growth on disposable rubber catheters and limited biofilm formation within 24 h. Cellular infection experiments showed that pretreatment phage could effectively inhibit UPEC adhesion to bladder epithelial cells with safety and reduce the cellular damage caused by UPEC. Our results suggest that vB_EcoP_P64441 is a novel and effective phage for UPEC inhibition with promising applications.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"609 ","pages":"Article 110570"},"PeriodicalIF":2.8,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143934833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role and implication of rotavirus VP8∗ in viral infection and vaccine development 轮状病毒VP8 *在病毒感染和疫苗研制中的作用和意义

IF 2.8 3区医学

Virology Pub Date : 2025-05-08 DOI: 10.1016/j.virol.2025.110563

Shalini Soni , Michael A. Kennedy , Dan Wang , Feng Li

{"title":"The role and implication of rotavirus VP8∗ in viral infection and vaccine development","authors":"Shalini Soni , Michael A. Kennedy , Dan Wang , Feng Li","doi":"10.1016/j.virol.2025.110563","DOIUrl":"10.1016/j.virol.2025.110563","url":null,"abstract":"<div><div>Rotaviruses (RVs) are major causative agents of diarrhea in both humans and animals worldwide. Despite the successful development of live attenuated vaccines, the efficacy of these vaccines remains low in developing countries and RV infections still result in more than 200,000 deaths in children under 5 years old globally each year. These viruses are also an enteric pathogen for agricultural animals and have caused substantial economic losses annually to the animal livestock industry. Frequent reassortment and the emergence of new RV strains continue to pose a significant challenge to human and agricultural animal health. Attachment to susceptible cells by recognizing cell surface glycans is the first step of the RV lifecycle, which is directed by the RV spike protein VP8∗. VP8∗-host glycan receptor interactions are thought to be strain-specific and play an important role in RV replication fitness, tropism, and cross-species transmission. This review will summarize the current understanding of the roles of VP8∗ in engagement of glycan receptors and its functional consequences in impacting RV replication fitness and host ranges. The current progress towards developing a VP8∗-based RV vaccine is also discussed in the review.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"609 ","pages":"Article 110563"},"PeriodicalIF":2.8,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The guinea pig as a relevant preclinical model in the rat race for a vaccine against congenital cytomegalovirus infection 豚鼠作为抗先天性巨细胞病毒感染疫苗大鼠竞赛的相关临床前模型

IF 2.8 3区医学

Virology Pub Date : 2025-05-06 DOI: 10.1016/j.virol.2025.110560

K. Yeon Choi, Alistair McGregor

{"title":"The guinea pig as a relevant preclinical model in the rat race for a vaccine against congenital cytomegalovirus infection","authors":"K. Yeon Choi, Alistair McGregor","doi":"10.1016/j.virol.2025.110560","DOIUrl":"10.1016/j.virol.2025.110560","url":null,"abstract":"<div><div>Cytomegalovirus (CMV) is a leading cause of congenital disease in newborns causing cognitive impairment and hearing loss as a result of placental and fetal infection. Primary infection establishes a life-time persistent viral state with potential for severe disease in immune suppressed individuals. Natural convalescent immunity does not prevent re-infection or congenital CMV (cCMV) by a new viral strain. Despite decades of research, an effective vaccine against cCMV remains an elusive goal. The guinea pig is an important model for various human diseases and the only small-animal model for cCMV. Human CMV (HCMV) is highly species-specific, and animal studies require species-specific CMV. Guinea pig cytomegalovirus (GPCMV) exhibits similarities to HCMV for tissue tropism, cell entry pathways, receptors and functional homolog proteins as pathogenicity factors or vaccine target antigens. Consequently, studies in this model potentially enable a better understanding of CMV disease and preclinical development of therapeutic and intervention strategies against cCMV and cross strain protection. This review provides a summary of CMV research carried out in this model and advancements made towards the development of an effective cCMV vaccine.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"610 ","pages":"Article 110560"},"PeriodicalIF":2.8,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144123446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycoviruses and their ecological impacts on fungi 分枝病毒及其对真菌的生态影响

IF 2.8 3区医学

Virology Pub Date : 2025-05-06 DOI: 10.1016/j.virol.2025.110562

Mingde Wu , Guoqing Li

引用次数: 0

The PB subunit vaccine evokes immune response against fox encephalitis PB亚单位疫苗可引起对狐脑炎的免疫应答

IF 2.8 3区医学

Virology Pub Date : 2025-04-30 DOI: 10.1016/j.virol.2025.110561

Jinfeng Xu , Boyang Zhang , Beng Wang , Zhao Ye , Baishuang Yin , Yanzhu Zhu

{"title":"The PB subunit vaccine evokes immune response against fox encephalitis","authors":"Jinfeng Xu , Boyang Zhang , Beng Wang , Zhao Ye , Baishuang Yin , Yanzhu Zhu","doi":"10.1016/j.virol.2025.110561","DOIUrl":"10.1016/j.virol.2025.110561","url":null,"abstract":"<div><div>Canine adenovirus type 1 (CAdV-1) is an important pathogen for fox encephalitis, and posing a severe threat to the global fox farming industry. Although live CAdV-2 vaccines are currently available, its defect remains a considerable challenge. It is vital to develop a safe subunit vaccine. This study aimed to evaluate the immunogenicity and safety of the penton base (PB) subunit vaccine in fox with CAdV-1, for which little is known. Bioinformatics methods were used to design a recombinant protein vaccine based on conserved regions of protein consensus sequences in PB protein. The E.coli prokaryotic expression system was utilized to enhance protein expression, solubility and immunogenicity. After the PB proteins of CAdV-1 were successfully expressed, the mice and silver foxes were immunized. The specific antibody, neutralizing antibody titters, T lymphocyte proliferation, cytokine, tissue histopathology, virus shedding, and the survival rate were investigated. The results showed that the PB subunit vaccine induced the production of antibodies in mice at 7, 14, 21, 30, 60 d post-vaccination (dpv). Moreover, the neutralizing antibody level of the PB group was higher than that in the Knob group at 21 and 28 dpv. It indicates that PB subunit vaccine elicits immunity. In the silver fox, PB subunit vaccine induced the production of antibodies at 30 dpv. Moreover, the neutralizing antibody level in PB group was higher than that in the CAdV-2 vaccine group at 30 dpv. PB subunit vaccine conferred complete protection in fox against CAdV-1. The tissue histopathology suggested that the PB subunit vaccine was more effective in blocking the pathogenicity of CAdV-1. In addition, the PB subunit vaccine further reduced the virus shedding in silver foxes than the CAdV-2 vaccine. The results suggest that this vaccine could be a promising candidate for preventing CAdV-1 in foxes.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"609 ","pages":"Article 110561"},"PeriodicalIF":2.8,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring suramin's antiviral effects: targeting Lymphocytic Choriomeningitis virus cell entry and replication/transcription processes 探索苏拉明的抗病毒作用：针对淋巴细胞性脉络丛脑膜炎病毒的细胞进入和复制/转录过程

IF 2.8 3区医学

Virology Pub Date : 2025-04-30 DOI: 10.1016/j.virol.2025.110557

Shuzo Urata , Tomoko Tsuruta , Manabu Igarashi

引用次数: 0

Exploring deep learning in phage discovery and characterization 探索深度学习在噬菌体发现和表征中的应用

IF 2.8 3区医学

Virology Pub Date : 2025-04-29 DOI: 10.1016/j.virol.2025.110559

Monyque Karoline de Paula Silva , Vitória Yumi Uetuki Nicoleti , Barbara da Paixão Perez Rodrigues , Ademir Sergio Ferreira Araujo , Joel Henrique Ellwanger , James Moraes de Almeida , Leandro Nascimento Lemos

{"title":"Exploring deep learning in phage discovery and characterization","authors":"Monyque Karoline de Paula Silva , Vitória Yumi Uetuki Nicoleti , Barbara da Paixão Perez Rodrigues , Ademir Sergio Ferreira Araujo , Joel Henrique Ellwanger , James Moraes de Almeida , Leandro Nascimento Lemos","doi":"10.1016/j.virol.2025.110559","DOIUrl":"10.1016/j.virol.2025.110559","url":null,"abstract":"<div><div>Bacteriophages, or bacterial viruses, play diverse ecological roles by shaping bacterial populations and also hold significant biotechnological and medical potential, including the treatment of infections caused by multidrug-resistant bacteria. The discovery of novel bacteriophages using large-scale metagenomic data has been accelerated by the accessibility of deep learning (Artificial Intelligence), the increased computing power of graphical processing units (GPUs), and new bioinformatics tools. This review addresses the recent revolution in bacteriophage research, ranging from the adoption of neural network algorithms applied to metagenomic data to the use of pre-trained language models, such as BERT, which have improved the reconstruction of viral metagenome-assembled genomes (vMAGs). This article also discusses the main aspects of bacteriophage biology using deep learning, highlighting the advances and limitations of this approach. Finally, prospects of deep-learning-based metagenomic algorithms and recommendations for future investigations are described.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"609 ","pages":"Article 110559"},"PeriodicalIF":2.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0