Virology最新文献_第2页

Insights into the different mechanisms of Autophagy and Apoptosis mediated by Morbilliviruses 深入了解由变形病毒介导的自噬和凋亡的不同机制。

IF 2.8 3区医学

Virology Pub Date : 2025-02-01 DOI: 10.1016/j.virol.2024.110371

Rashmi Singh, Sharad Kumar Gaur, Rakhi Nagar, Rajeev Kaul

引用次数: 0

Epidemiology and genotypic diversity of duck hepatitis B virus identified from waterfowl in partial areas of Guangdong province, Southern China

IF 2.8 3区医学

Virology Pub Date : 2025-02-01 DOI: 10.1016/j.virol.2025.110416

Xinyan Yao , Chaoxiang Jia , Anqi Li , Ting Qin , Dai Peng , Yingqian Han , Shuang Guo , Kai Zhong , Guoyu Yang , Yueying Wang , Heping Li

{"title":"Epidemiology and genotypic diversity of duck hepatitis B virus identified from waterfowl in partial areas of Guangdong province, Southern China","authors":"Xinyan Yao , Chaoxiang Jia , Anqi Li , Ting Qin , Dai Peng , Yingqian Han , Shuang Guo , Kai Zhong , Guoyu Yang , Yueying Wang , Heping Li","doi":"10.1016/j.virol.2025.110416","DOIUrl":"10.1016/j.virol.2025.110416","url":null,"abstract":"<div><div>Duck Hepatitis B virus (DHBV) infection model is extensively utilized as an animal model for studying human hepatitis B virus infection and for comparative research. 557 liver samples from geese and ducks were collected in parts of Guangdong province, southern China. The overall prevalence of DHBV was 45.6% (254/557) in all samples. And the 27 complete genome sequences of DHBV strains in this study share 89.6%–100% genome-wide pairwise identity with previously identified DHBV genomes. Notably, DHBV-1, DHBV-2 and DHBV-3 of were found co-circulating among the waterfowl population in parts of Guangdong. More importantly, seven out of the 16 recombination events were determined involved DHBV sequences obtained in this study as major parent and minor parent, suggesting DHBV strains from Guangdong province play an important role in recombination events. Additionally, purifying selection was the dominant evolutionary pressure acting on the genomes of DHBV.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"603 ","pages":"Article 110416"},"PeriodicalIF":2.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143025973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antiviral activity of ribosome inactivating proteins for management of plant viral infection

IF 2.8 3区医学

Virology Pub Date : 2025-02-01 DOI: 10.1016/j.virol.2025.110403

Pooja Bhardwaj , Baswaraj Raigond , Pinky Raigond , Ambika Verma , Gaurav Verma , Tarvinder Kochhar , Parashuram Patroti , I.K. Das , C Tara Satyavathi

引用次数: 0

The regulatory role of Epinephelus Coioides miR-21 in the infection and replication of iridovirus SGIV Epinephelus Coioides miR-21 在虹彩病毒 SGIV 感染和复制中的调控作用。

IF 2.8 3区医学

Virology Pub Date : 2025-02-01 DOI: 10.1016/j.virol.2024.110325

Wei Huang , Pin-Hong Li , Ru-Nan He , Yu-Rong Lei , Cui-Fen Huang , Yun-Xiang Lin , Yin-Mei Lan , Zi-An Chen , Ze-Peng Zhang , Qi-Wei Qin , Yan-Hong Sun

{"title":"The regulatory role of Epinephelus Coioides miR-21 in the infection and replication of iridovirus SGIV","authors":"Wei Huang , Pin-Hong Li , Ru-Nan He , Yu-Rong Lei , Cui-Fen Huang , Yun-Xiang Lin , Yin-Mei Lan , Zi-An Chen , Ze-Peng Zhang , Qi-Wei Qin , Yan-Hong Sun","doi":"10.1016/j.virol.2024.110325","DOIUrl":"10.1016/j.virol.2024.110325","url":null,"abstract":"<div><div>Iridovirus SGIV is a highly pathogenic virus of fish that can cause more than 90% mortality in <em>Epinephelus coioides</em>, a marine farmed fish in South China. miRNAs can be involved in regulating the development of virus-induced diseases. In this study, SGIV infection could significantly inhibit the expression of <em>E. coioides</em> miR-21. And, overexpressing miR-21 could inhibit the expressions of viral key genes (ICP18, VP19, LITAF and MCP), SGIV-induced CPE, and viral titers. Overexpression of miR-21 promoted the promoter activity of AP-1/NF-κB, SGIV-induced apoptosis, and activities of caspase 3/9. Inhibiting miR-21 could produce the opposite results. <em>E. Coioides</em> PDCD4 is a targeting gene of miR-21, and we speculate that PDCD4 downregulation may, at least in part, explain the observed antiviral effects. These studies indicate that miR-21 could inhibit the infection and replication of SGIV, which might provide a molecular basis for further exploring the mechanism of SGIV invasion.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"603 ","pages":"Article 110325"},"PeriodicalIF":2.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The UP1 domain is essential for the facilitation effect of HnRNP A1 on PRRSV-2 replication UP1结构域对于HnRNP A1对PRRSV-2复制的促进作用至关重要。

IF 2.8 3区医学

Virology Pub Date : 2025-02-01 DOI: 10.1016/j.virol.2024.110378

Huiyuan Jing , Ying Liu , Zhifeng Peng , Erzhen Duan , Jie Liu , Yujin Lv , Yanting Sun , Wang Dong , Xianghui Li , Jinhe Wang , Sufang Cao , Haihua Wang , Yan Zhang , Huawei Li

{"title":"The UP1 domain is essential for the facilitation effect of HnRNP A1 on PRRSV-2 replication","authors":"Huiyuan Jing , Ying Liu , Zhifeng Peng , Erzhen Duan , Jie Liu , Yujin Lv , Yanting Sun , Wang Dong , Xianghui Li , Jinhe Wang , Sufang Cao , Haihua Wang , Yan Zhang , Huawei Li","doi":"10.1016/j.virol.2024.110378","DOIUrl":"10.1016/j.virol.2024.110378","url":null,"abstract":"<div><div>Porcine reproductive and respiratory syndrome virus (PRRSV) infection causes reproductive failure and respiratory distress and is a serious threat to the swine industry, given its continuous and rapid emergence. The knowledge of viral-host interaction could facilitate anti-PRRSV drug development. HnRNP A1 is an abundantly expressed protein which associates with RNA metabolic processes and plays multifarious roles during the reproduction cycle of multiple viruses. However, the function of porcine HnRNP A1 in PRRSV-2 replication is still unknown. Herein, HnRNP A1 was identified as a nucleocapsid (N)-binding protein for PRRSV-2. Overexpression of porcine HnRNP A1 promoted the expression of viral RNA, and viral proteins, corresponding to enhanced virus titers. While deletion of the UP1 domain abolished the HnRNP A1-mediated enhancement of PRRSV-2 replication. In addition, HnRNP A1-silencing confirmed its pro-viral effect on PRRSV-2 infectivity in porcine alveolar macrophages (PAMs). RNA pull-down and RNA immunoprecipitation verification confirmed that the UP1 domain is important for the recognition of the guanine-rich sequence (GRS) in PRRSV-2 negative RNA. Eventually, supplementation with TMPyP4, a G4 ligand, efficiently provokes the release of HnRNP A1 from GRS, thereby limiting PRRSV-2 replication. Together, these findings help to inform the mechanism by which HnRNP A1 accelerates PRRSV-2 replication, and facilitate antiviral drug design.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"603 ","pages":"Article 110378"},"PeriodicalIF":2.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142901517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in the diagnostic techniques of African swine fever 非洲猪瘟诊断技术进展。

IF 2.8 3区医学

Virology Pub Date : 2025-02-01 DOI: 10.1016/j.virol.2024.110351

Xin Zhang , Lei Zhou , Xinna Ge , Peng Gao , Qiongqiong Zhou , Jun Han , Xin Guo , Yongning Zhang , Hanchun Yang

{"title":"Advances in the diagnostic techniques of African swine fever","authors":"Xin Zhang , Lei Zhou , Xinna Ge , Peng Gao , Qiongqiong Zhou , Jun Han , Xin Guo , Yongning Zhang , Hanchun Yang","doi":"10.1016/j.virol.2024.110351","DOIUrl":"10.1016/j.virol.2024.110351","url":null,"abstract":"<div><div>African swine fever (ASF) is a highly contagious disease of pigs caused by African swine fever virus, which poses a huge threat to the global swine industry and is therefore listed as a notifiable disease by the World Organization for Animal Health. Due to the global lack of safe and efficacious vaccines and therapeutic drugs, early diagnosis of cases, whether on-site or laboratory, are crucial for the prevention and control of ASF. Therefore, rapid and reliable diagnosis and detection have become the main means to combat ASF. In this paper, various diagnostic techniques developed globally for ASF diagnosis, including etiological, molecular biological and serological diagnostic techniques, as well as conventional and novel diagnostic techniques, were comprehensively reviewed, and the main advantages and disadvantages of currently commonly used diagnostic techniques were introduced. It is expected that this paper will provide references for selecting appropriate ASF diagnostic techniques in different application scenarios, and also provide directions for the development of innovative diagnostic techniques for ASF in the future.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"603 ","pages":"Article 110351"},"PeriodicalIF":2.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complex interplay: The interactions between citrus tristeza virus and its host 复杂相互作用：柑橘tristeza病毒与其宿主之间的相互作用。

IF 2.8 3区医学

Virology Pub Date : 2025-02-01 DOI: 10.1016/j.virol.2024.110388

Maryam Khalilzadeh , Dirk Jacobus Aldrich , Hans Jacob Maree , Amit Levy

引用次数: 0

Expression and purification of PNGase F protein in yeast and its anti-PRV activity PNGase F蛋白在酵母中的表达纯化及其抗prv活性研究。

IF 2.8 3区医学

Virology Pub Date : 2025-02-01 DOI: 10.1016/j.virol.2025.110393

Haonan Zhang , Yu Jiang , Gang Ding , Jingyu Chen , Yuda Liu , Furong Wang , Xiaolan Yu

{"title":"Expression and purification of PNGase F protein in yeast and its anti-PRV activity","authors":"Haonan Zhang , Yu Jiang , Gang Ding , Jingyu Chen , Yuda Liu , Furong Wang , Xiaolan Yu","doi":"10.1016/j.virol.2025.110393","DOIUrl":"10.1016/j.virol.2025.110393","url":null,"abstract":"<div><div>Pseudorabies virus (Pseudorabiesvirus, PRV) has caused huge economic losses to the global pig industry. In recent years, it has been reported that there are PRV mutants, but the traditional vaccine can not completely prevent or control the infection of PRV, so there is an urgent need to develop new broad-spectrum anti-disease drugs for prevention and treatment. PNGase F from bacteria can catalyze the hydrolysis of oligosaccharides linked to asparagine residues on peptides, so we speculate that PNGase F can inhibit virus infection by removing the glycosylation of virus membrane glycoproteins. In this study, PNGase F protein was highly expressed and purified in Pichia pastoris, and the deglycosylation activity of PNGase F expressed in Pichia pastoris was verified. In vitro, 15 μM could significantly inhibit the proliferation of virus in cells. The results of cytotoxicity test showed that PNGase F was not toxic to many cells. To further evaluate the effect of PNGase F in different stages of virus infection, it was found that PNGase F had significant inhibitory effect on virus adsorption and invasion. In vivo experiments in mice, PNGase F could significantly inhibit the replication of PRV Ea strain in mice and inhibit PRV, reduced brain lesions. Our experiments show that PNGase F expressed by yeast can inhibit PRV infection in vitro and in vitro, and its inhibitory mechanism is preliminarily discussed, which can provide a new reference for the development of broad-spectrum antiviral drugs based on PNGase F.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"603 ","pages":"Article 110393"},"PeriodicalIF":2.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143019333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

STAT1 and herpesviruses: Making lemonade from lemons

IF 2.8 3区医学

Virology Pub Date : 2025-02-01 DOI: 10.1016/j.virol.2024.110364

Erika R. Johansen, Vera L. Tarakanova

引用次数: 0

Deciphering the hepatitis E virus ORF1: Functional domains, protein processing, and patient-derived mutations 解密戊型肝炎病毒 ORF1：功能域、蛋白质加工和源自患者的突变。

IF 2.8 3区医学

Virology Pub Date : 2025-02-01 DOI: 10.1016/j.virol.2024.110350

Fei Zhang , Ling-Dong Xu , Shiying Wu , Bin Wang , Pinglong Xu , Yao-Wei Huang

引用次数: 0