Virology最新文献_第2页

Application of reverse genetics system to Chikungunya virus study 在基孔肯雅病毒研究中应用反向遗传学系统

IF 2.8 3区医学

Virology Pub Date : 2025-02-27 DOI: 10.1016/j.virol.2025.110465

Youichi Suzuki

引用次数: 0

vB_Ent31 bacteriophage may combat Enterobacter cloacae infections with macrophage modulating activity

IF 2.8 3区医学

Virology Pub Date : 2025-02-25 DOI: 10.1016/j.virol.2025.110463

Mengyuan Li , Jiayue Ma , Xiaoshuang Feng , Naijin Zheng , Hong Li , Xue Chi , Xiang Ma , Yanqiong Tang , Juanjuan Li , Zhu Liu

{"title":"vB_Ent31 bacteriophage may combat Enterobacter cloacae infections with macrophage modulating activity","authors":"Mengyuan Li , Jiayue Ma , Xiaoshuang Feng , Naijin Zheng , Hong Li , Xue Chi , Xiang Ma , Yanqiong Tang , Juanjuan Li , Zhu Liu","doi":"10.1016/j.virol.2025.110463","DOIUrl":"10.1016/j.virol.2025.110463","url":null,"abstract":"<div><div>Multidrug-resistant organisms (MDRO), including MDR <em>Enterobacter</em> cloacae, may emerge due to the extensive usage of antibiotics and threaten the lives of millions of people around the world. Developing new antibiotic-free strategies to combat <em>E. cloacae</em> infections and curb the spread of drug-resistant genes is crucial. Bacteriophage therapy has garnered widespread attention as a promising approach to tackle bacterial infections. Herein, we isolated a specific bacteriophage (vB_Ent31) targeting <em>E. cloacae</em> from sewage using <em>E. cloacae</em> Ent31 as the host bacterium. vB_Ent31 is a tadpole-like phage with double-stranded DNA belonging to the <em>Siphoviridae</em> family. It exhibits narrow-spectrum activity against <em>Enterobacter</em> spp. and remains stable across a temperature range of 4–50 °C and pH 4 to 11. Significantly, vB_Ent31 prevents proliferation of Ent31 and inhibits inflammation, which further accelerate wound healing. Our findings suggest that bacteriophage therapy could offer an alternative to combating drug-resistant bacteria.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"605 ","pages":"Article 110463"},"PeriodicalIF":2.8,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143511328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of lactylation in virology

IF 2.8 3区医学

Virology Pub Date : 2025-02-25 DOI: 10.1016/j.virol.2025.110466

Sirui Wang , Leiliang Zhang

引用次数: 0

Longitudinal humoral immunity against SARS-CoV-2 Spike following infection in individuals from Cameroon

IF 2.8 3区医学

Virology Pub Date : 2025-02-25 DOI: 10.1016/j.virol.2025.110467

Mehdi Benlarbi , Dell-Dylan Kenfack , Katrina Dionne , Maxime Côté-Chenette , Guillaume Beaudoin-Bussières , Étienne Bélanger , Shilei Ding , Oumarou H. Goni , Yannick F. Ngoume , Alexandra Tauzin , Halima Medjahed , Elodie Ghedin , Ralf Duerr , Andrés Finzi , Marcel Tongo

{"title":"Longitudinal humoral immunity against SARS-CoV-2 Spike following infection in individuals from Cameroon","authors":"Mehdi Benlarbi , Dell-Dylan Kenfack , Katrina Dionne , Maxime Côté-Chenette , Guillaume Beaudoin-Bussières , Étienne Bélanger , Shilei Ding , Oumarou H. Goni , Yannick F. Ngoume , Alexandra Tauzin , Halima Medjahed , Elodie Ghedin , Ralf Duerr , Andrés Finzi , Marcel Tongo","doi":"10.1016/j.virol.2025.110467","DOIUrl":"10.1016/j.virol.2025.110467","url":null,"abstract":"<div><div>In May 2023 the World Health Organization (WHO) declared the end of COVID-19 as a public health emergency. Seroprevalence studies performed in African countries, such as Cameroon, depicted a much higher COVID-19 burden than reported by the WHO. To better understand humoral responses kinetics following infection, we enrolled 333 participants from Yaoundé, Cameroon between March 2020 and January 2022. We measured the levels of antibodies targeting the SARS-CoV-2 receptor-binding-domain (RBD) and the Spike glycoproteins of Delta, Omicron BA.1 and BA.4/5 and the common cold coronavirus HCoV-OC43. We also evaluated plasma capacity to neutralize authentic SARS-CoV-2 virus and to mediate Antibody-Dependent Cellular Cytotoxicity (ADCC). Most individuals mounted a strong antibody response against SARS-CoV-2 Spike. Plasma neutralization waned faster than anti-Spike binding and ADCC. We observed differences in humoral responses by age and circulating variants. Altogether, we show a global overview of antibody dynamics and functionality against SARS-CoV-2 in Cameroon.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"605 ","pages":"Article 110467"},"PeriodicalIF":2.8,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143529026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifunctional noncoding regions in the mammarenavirus genome

IF 2.8 3区医学

Virology Pub Date : 2025-02-24 DOI: 10.1016/j.virol.2025.110464

Masaharu Iwasaki

引用次数: 0

Shared and unique mechanisms of RNAi-mediated antiviral immunity in C. elegans

IF 2.8 3区医学

Virology Pub Date : 2025-02-21 DOI: 10.1016/j.virol.2025.110459

Teng Yan , Rui Lu

{"title":"Shared and unique mechanisms of RNAi-mediated antiviral immunity in C. elegans","authors":"Teng Yan , Rui Lu","doi":"10.1016/j.virol.2025.110459","DOIUrl":"10.1016/j.virol.2025.110459","url":null,"abstract":"<div><div>Small interfering RNAs (siRNAs), generated by Dicer proteins, play a pivotal role in antiviral immunity in eukaryotes. Dicer proteins also produce microRNAs (miRNAs), a class of endogenous small non-coding RNAs that regulate essential cellular functions through post-transcriptional mechanisms. In plants and insects, multiple Dicer proteins are produced and deployed to separately manage the biogenesis of antiviral siRNAs and miRNAs. This separation ensures that viral infections, especially the production of viral RNAi suppressors, do not severely compromise host growth or development. In contrast, nematode worms, such as <em>Caenorhabditis elegans,</em> rely on a single Dicer protein to produce both types of small RNAs. Probably as a strategy to mitigate the potential disruption of miRNA production by viral infections, nematodes have evolved distinct strategies for generating primary and secondary siRNAs for antiviral defense. This review explores the shared and unique features of siRNA-mediated antiviral immunity in <em>Caenorhabditis elegans</em>, shedding light on the specialized adaptations that enable robust antiviral defenses without compromising miRNA-mediated function.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"605 ","pages":"Article 110459"},"PeriodicalIF":2.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143511330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunity responses as checkpoints for efficient transmission of begomoviruses by whiteflies

IF 2.8 3区医学

Virology Pub Date : 2025-02-21 DOI: 10.1016/j.virol.2025.110462

Ilana Kuzminsky , Murad Ghanim

{"title":"Immunity responses as checkpoints for efficient transmission of begomoviruses by whiteflies","authors":"Ilana Kuzminsky , Murad Ghanim","doi":"10.1016/j.virol.2025.110462","DOIUrl":"10.1016/j.virol.2025.110462","url":null,"abstract":"<div><div>Begomoviruses are a group of single stranded DNA plant viruses exclusively transmitted by the sweet potato whitefly <em>Bemisia tabaci</em> in a persistent, circulative manner. After acquisition from plant phloem, this group of viruses circulate and are retained within the whitefly, interacting with tissues, cells and molecular pathways for maintaining the safety of the infective intact virions, by exploiting cellular mechanisms and avoiding degradation by the insect immune responses. During retention, the virions are internalized in the midgut cells, exit and spend hours-days in the hemolymph and cross into salivary gland cells, before transmission. Destroying this group of viruses by the insect immune system seems inefficient for the most part, by examining their very efficient transmission. Thus, within the various sites along the transmission pathway especially in the midgut, it is thought that the immune system with its various layers is activated for avoiding the damage caused by the viruses on one hand, and for ensuring their safe circulation and transmission on the other hand. Begomoviruses have evolved mechanisms for counteracting and exploiting the activated immune system for their safe translocation within the whitefly. In this review, we discuss the various levels of immunity activated against begomoviruses in <em>B. tabaci,</em> taking other pathogen-vector systems as examples and reflecting relevant components on the interactions between <em>B. tabaci</em> and Begomoviruses.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"605 ","pages":"Article 110462"},"PeriodicalIF":2.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143509476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Viral oncogenesis of δ-retroviruses, HTLV-1 and BLV, and recent advances in its diagnosis

IF 2.8 3区医学

Virology Pub Date : 2025-02-21 DOI: 10.1016/j.virol.2025.110461

Md Belal Hossain , Benjy Jek Yang Tan , Yorifumi Satou

引用次数: 0

Altered receptor-binding specificity of gull-adapted H13 avian influenza viruses corresponds to their unique host preferences

IF 2.8 3区医学

Virology Pub Date : 2025-02-21 DOI: 10.1016/j.virol.2025.110460

Rio Harada , Takahiro Hiono , Manabu Igarashi , Daiki Kobayashi , Hinako Ban , Norikazu Isoda , Yoshihiro Sakoda

{"title":"Altered receptor-binding specificity of gull-adapted H13 avian influenza viruses corresponds to their unique host preferences","authors":"Rio Harada , Takahiro Hiono , Manabu Igarashi , Daiki Kobayashi , Hinako Ban , Norikazu Isoda , Yoshihiro Sakoda","doi":"10.1016/j.virol.2025.110460","DOIUrl":"10.1016/j.virol.2025.110460","url":null,"abstract":"<div><div>Avian influenza viruses (AIVs) recognize α2-3 sialosides as receptors. Previous studies showed that the structural diversity within α2-3 sialosides is related to the host specificity of AIVs. H13 AIVs are primarily isolated from gulls, although almost all AIV subtypes have been isolated from ducks, the natural hosts of AIVs. To elucidate the molecular basis of the host specificity of H13 viruses to gulls, the receptor-binding specificity of H13 hemagglutinins (HAs) and the distribution of viral receptors in gulls were investigated. The results revealed that recombinant HA (rHA) of H13 viruses had a binding preference for fucosylated α2-3 sialosides, which were distributed widely in the respiratory tract and intestines of gulls but not in the colon of ducks. Moreover, the receptor-binding specificity of mutant rHAs revealed that amino acids in the 130-loop and at position 227 of H13 HA were critical for the preference for fucosylated α2-3 sialosides. The results of the present study suggest that the binding specificity of H13 HA to fucosylated α2-3 sialosides is a key factor for the host susceptibility of H13 viruses to gulls.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"605 ","pages":"Article 110460"},"PeriodicalIF":2.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143474925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing the virological response to direct-acting antiviral therapies in the HBV cure programme

IF 2.8 3区医学

Virology Pub Date : 2025-02-21 DOI: 10.1016/j.virol.2025.110458

James Lok , James M. Harris , Ivana Carey , Kosh Agarwal , Jane A. McKeating

引用次数: 0