The characteristics and genomic analysis of Vibrio cholerae phage VP1

IF 2.4 3区医学 Q3 VIROLOGY

Virology Pub Date : 2025-09-06 DOI:10.1016/j.virol.2025.110673

Xi Chen, Meiying Yan, Rujing Sun, Biao Kan, Fenxia Fan

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引用次数: 0

Abstract

The lytic bacteriophage VP1 specifically targets Vibrio cholerae (V. cholerae), showing excellent therapeutic potential. It features a 55-min latency period, large burst size (255 virions/cell), and remarkable stability (pH 5–12). Genomic sequencing revealed a double-stranded DNA genome of 42,845 bp with a G + C content of 45.49 %. Among the 61 predicted open reading frames (ORFs), 55 were validated by proteomic analysis, while only 16 exhibited functional homology to known proteins in public databases. The genome lacks tRNA genes, CRISPR-associated elements, virulence factors, or antibiotic resistance genes, underscoring its safety profile. It effectively lyses O1 and O139 V. cholerae strains while showing no activity against other Vibrio species. Comparative genomic analysis showed VP1 shares limited similarity (<71.39 % identity) with five Vibrio phages. Phylogenetic analysis of terminase large subunit (TerL) and major capsid protein (MCP) genes further confirmed VP1's distinct evolutionary position, clustering with V. splendidus phage but forming a separate clade from established Caudovirales families. Given its potent lytic activity, genomic stability, unique phylogenetic position, and absence of harmful genetic elements, VP1 represents a putative novel phage lineage within Podovirales.

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霍乱弧菌噬菌体VP1的特征及基因组分析

裂解噬菌体VP1特异性靶向霍乱弧菌（V. cholae），具有良好的治疗潜力。它的特点是潜伏期55分钟，爆发大小大（255个病毒粒子/细胞），稳定性好（pH值5-12）。基因组测序结果显示，双链DNA基因组长度为42,845 bp， G + C含量为45.49%。在61个预测的开放阅读框（orf）中，55个通过蛋白质组学分析得到验证，而只有16个与公共数据库中已知的蛋白质具有功能同源性。基因组缺乏tRNA基因、crispr相关元件、毒力因子或抗生素耐药基因，强调其安全性。它能有效地裂解O1和O139霍乱弧菌菌株，而对其他弧菌没有活性。比较基因组分析显示VP1与5种噬菌体具有有限的相似性（71.39%的同源性）。末端酶大亚基（TerL）和主要衣壳蛋白（MCP）基因的系统发育分析进一步证实了VP1的独特进化地位，它与V. splendidus噬菌体聚类，但与已建立的Caudovirales家族形成一个独立的分支。鉴于其强大的裂解活性、基因组稳定性、独特的系统发育位置和缺乏有害遗传元件，VP1代表了Podovirales中一个假定的新型噬菌体谱系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Virology 医学-病毒学

CiteScore

6.00

自引率

0.00%

发文量

157

审稿时长

50 days

期刊介绍： Launched in 1955, Virology is a broad and inclusive journal that welcomes submissions on all aspects of virology including plant, animal, microbial and human viruses. The journal publishes basic research as well as pre-clinical and clinical studies of vaccines, anti-viral drugs and their development, anti-viral therapies, and computational studies of virus infections. Any submission that is of broad interest to the community of virologists/vaccinologists and reporting scientifically accurate and valuable research will be considered for publication, including negative findings and multidisciplinary work.Virology is open to reviews, research manuscripts, short communication, registered reports as well as follow-up manuscripts.