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Mutation and trans-activation analyses suggest novel functional motifs for AC2 of the begomovirus Sri Lankan cassava mosaic virus 突变和反式激活分析提示了begomvirus斯里兰卡木薯花叶病毒AC2的新功能基序。

IF 2.4 3区医学

Virology Pub Date : 2026-02-01 Epub Date: 2025-12-04 DOI: 10.1016/j.virol.2025.110772

Nabanita Gogoi , Kanika Gupta , Rohit Kumar , Fauzia Zarreen, Indranil Dasgupta

{"title":"Mutation and trans-activation analyses suggest novel functional motifs for AC2 of the begomovirus Sri Lankan cassava mosaic virus","authors":"Nabanita Gogoi , Kanika Gupta , Rohit Kumar , Fauzia Zarreen, Indranil Dasgupta","doi":"10.1016/j.virol.2025.110772","DOIUrl":"10.1016/j.virol.2025.110772","url":null,"abstract":"<div><div>This study was aimed to have a better understanding of the functional domains of the product of <em>AC2</em>, a protein having a <em>trans</em>-activation function on the late promoters of begomoviruses. The bipartite begomovirus Sri Lankan cassava mosaic virus (SLCMV) was chosen for this study. Infectious SLCMV clones carrying mutations in <em>AC2</em> were analysed for infectivity, accumulation of viral DNA and <em>trans</em>-activation of the CP promoter in the experimental host <em>Nicotiana benthamiana</em>. The mutations were designed to express truncated AC2 proteins (containing N-terminal 36, N-terminal 101 or C-terminal 22 out of the 135 amino acid residues of the complete protein) or single amino acid replacements targeting the conserved “cysteine-rich” and the “Zn-finger” domains, implicated in <em>trans</em>-activation by AC2 of other begomoviruses. In the inoculated leaves, the DNA of all the above mutants, except the one expressing the C-terminal 22 amino acid residues accumulated at wild type levels. In newly-emerged leaves, only the DNA of the mutant expressing N-terminal 101 residues showed a delayed accumulation compared to the wild type and produced mild symptoms in the inoculated plants. The capability of <em>trans</em>-activation of CP promoter was abolished in all <em>AC2</em> mutants except in the one expressing the N-terminal 101 residues, lacking the C-terminal <em>trans</em>-activation domain. The results suggest novel functional domains of the <em>AC2</em> product in SLCMV, not described before.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"615 ","pages":"Article 110772"},"PeriodicalIF":2.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145717217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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The antiviral activity of interferon-stimulated genes (ISGs) in influenza A virus infection 干扰素刺激基因（ISGs）在甲型流感病毒感染中的抗病毒活性

IF 2.4 3区医学

Virology Pub Date : 2026-02-01 Epub Date: 2025-10-28 DOI: 10.1016/j.virol.2025.110728

Aoxue Wang , Jiashu Hu , Qianqian Zhang , Yuying Zhang , Fanhua Wei

引用次数: 0

Groundwork for phage therapy: multi-faceted comparative analysis of lytic bacteriophages infecting uropathogenic Escherichia coli 噬菌体治疗的基础：溶菌感染尿路致病性大肠杆菌的多方面比较分析

IF 2.4 3区医学

Virology Pub Date : 2026-02-01 Epub Date: 2025-11-11 DOI: 10.1016/j.virol.2025.110738

Aleksandra Łukasiak , Wojciech Wesołowski , Julia Neumann , Natalia Lewandowska , Emilia Węglińska , Sylwia Bloch , Grzegorz Węgrzyn , Bożena Nejman-Faleńczyk

{"title":"Groundwork for phage therapy: multi-faceted comparative analysis of lytic bacteriophages infecting uropathogenic Escherichia coli","authors":"Aleksandra Łukasiak , Wojciech Wesołowski , Julia Neumann , Natalia Lewandowska , Emilia Węglińska , Sylwia Bloch , Grzegorz Węgrzyn , Bożena Nejman-Faleńczyk","doi":"10.1016/j.virol.2025.110738","DOIUrl":"10.1016/j.virol.2025.110738","url":null,"abstract":"<div><div>Phage therapy is a promising alternative for treating urinary tract infections (UTIs), particularly given the rapid emergence of antimicrobial resistance among uropathogenic <em>Escherichia coli</em> (UPEC). In this study, we review 98 reported lytic phages infecting UPEC, summarizing their morphology, host range, isolation sources, infection efficiency, progeny yield, adsorption rate, stability across pH and temperature ranges, biofilm-disrupting capacity, and other relevant characteristics. In addition, we conducted detailed bioinformatic and phylogenetic analyses of phage genomic sequences. This multi-faceted analysis highlights the considerable diversity and strong therapeutic potential of anti-UPEC phages, providing a solid scientific basis for their application in phage therapy. However, several limitations remain that hinder clinical translation. We discuss these challenges, drawing on examples from the literature, and outline that comprehensive physiological and genomic characterization, combined with standardized testing methodologies, can address current barriers to the effective use of anti-UPEC phages.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"615 ","pages":"Article 110738"},"PeriodicalIF":2.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145617371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamics of virus infection in a Wolbachia-infected host 沃尔巴克氏体感染宿主的病毒感染动力学

IF 2.4 3区医学

Virology Pub Date : 2026-02-01 Epub Date: 2025-11-27 DOI: 10.1016/j.virol.2025.110759

Angelique K. Asselin, Jan Engelstädter, Karyn N. Johnson

{"title":"Dynamics of virus infection in a Wolbachia-infected host","authors":"Angelique K. Asselin, Jan Engelstädter, Karyn N. Johnson","doi":"10.1016/j.virol.2025.110759","DOIUrl":"10.1016/j.virol.2025.110759","url":null,"abstract":"<div><div><em>Wolbachia</em> is a bacterium that blocks viruses and protects its arthropod hosts from infection. Whilst <em>Wolbachia</em> blocking is used as a strategy to prevent mosquito-borne disease, the mechanisms of <em>Wolbachia</em>-mediated viral blocking are not well characterised. Several mechanisms have been implicated, but it is often unclear their contribution to the overall blocking and how broadly they apply across host-virus-<em>Wolbachia</em> systems. <em>Wolbachia's</em> impact on viral dynamics and pathogenicity in individual <em>Drosophila</em> hosts was measured to explore what <em>Wolbachia</em>-mediated changes dominate the blocking phenotype. The empirical data was combined with a dynamical model to look at the impact of a series of parameters. This model described changes through time to susceptible cells, infected cells, and free virion particles. Results showed that <em>Wolbachia</em> reduced the accumulation of viral RNA and infectious viruses. The maximum amount of viral RNA was lower in individuals with <em>Wolbachia,</em> while the maximum amount of infectious virus was the same. These viral dynamics of <em>Wolbachia</em>-infected individuals could be recapitulated with a lower rate of viral infection of susceptible cells, lower viral release, a smaller number of susceptible cells or smaller burst size or various combinations of these processes. Experimental testing of the importance of these processes may be required to resolve their contribution to <em>Wolbachia</em>-mediated virus blocking. As further empirical data is added, the dynamical modelling approach may help guide decisions on which large impact mechanisms to focus on for functional approaches.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"615 ","pages":"Article 110759"},"PeriodicalIF":2.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145682666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Genomic characterization of the Vibrio parahaemolyticus strain CIBGEN003 and mid-stage RNA-Seq analysis during phage vB_Vp_PvVp04_M infection 副溶血性弧菌CIBGEN003的基因组特征及噬菌体vB_Vp_PvVp04_M感染中期RNA-Seq分析

IF 2.4 3区医学

Virology Pub Date : 2026-02-01 Epub Date: 2025-12-02 DOI: 10.1016/j.virol.2025.110769

Irais Ramírez-Sánchez, Alberto Peña-Rodríguez, Cristina Escobedo-Fregoso, Martín Ramírez-Orozco, Alexis Sebastián Martínez-López, Diana R. Barajas-Sandoval, Eduardo Quiroz-Guzmán

{"title":"Genomic characterization of the Vibrio parahaemolyticus strain CIBGEN003 and mid-stage RNA-Seq analysis during phage vB_Vp_PvVp04_M infection","authors":"Irais Ramírez-Sánchez, Alberto Peña-Rodríguez, Cristina Escobedo-Fregoso, Martín Ramírez-Orozco, Alexis Sebastián Martínez-López, Diana R. Barajas-Sandoval, Eduardo Quiroz-Guzmán","doi":"10.1016/j.virol.2025.110769","DOIUrl":"10.1016/j.virol.2025.110769","url":null,"abstract":"<div><div><em>Vibrio parahaemolyticus</em> is a primary etiological agent of acute hepatopancreatic necrosis disease (AHPND), which has caused substantial economic losses in the global aquaculture systems. Nowadays, antimicrobial resistance is a significant problem that makes producers search for alternative treatments to eradicate these infections; phages are an excellent strategy to address this issue. This study reported the genomic characterization of <em>V. parahaemolyticus</em> strain identified as <em>V. parahaemolyticus</em> CIBGEN003, isolated from a shrimp farm. We tested this strain against a Vibrio phage, to identify the genes involved in the infection of the phage vB_PvVP04_M in <em>V. parahaemolyticus</em> CIBGEN003 at 10 min after phage infection and before the lysis process. Phage genes transcribed at 10 min were associated to nucleotide metabolism and gene expression regulation; however, the expression of genes involved in virion structure and lysis remained unchanged. In addition, 22 bacterial genes were differentially transcribed at this time point, where upregulated genes are involved in cellular processes, including electron transfer and protein folding. In contrast downregulated genes were associated with nitrate assimilation and oxidative stress. This approach provided a better understanding of the transcriptional response to the bacterial-phage interaction before bacterial lysis.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"615 ","pages":"Article 110769"},"PeriodicalIF":2.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145682667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Spumigins produced by Nodularia spumigena are natural serine protease inhibitors with anti-SARS-CoV-2 activity Spumigins是一种天然丝氨酸蛋白酶抑制剂，具有抗sars - cov -2活性

IF 2.4 3区医学

Virology Pub Date : 2026-02-01 Epub Date: 2025-11-27 DOI: 10.1016/j.virol.2025.110763

Aleksandra Milewska , Robert Konkel , Markus Hoffmann , Stefan Pöhlmann , Artur Szczepański , Tony Fröhlich , Grzegorz Popowicz , Donata Overlinge , Hanna Mazur-Marzec , Krzysztof Pyrć

{"title":"Spumigins produced by Nodularia spumigena are natural serine protease inhibitors with anti-SARS-CoV-2 activity","authors":"Aleksandra Milewska , Robert Konkel , Markus Hoffmann , Stefan Pöhlmann , Artur Szczepański , Tony Fröhlich , Grzegorz Popowicz , Donata Overlinge , Hanna Mazur-Marzec , Krzysztof Pyrć","doi":"10.1016/j.virol.2025.110763","DOIUrl":"10.1016/j.virol.2025.110763","url":null,"abstract":"<div><div>COVID-19, caused by SARS-CoV-2, has led to significant morbidity and mortality worldwide. The pandemic has sparked extensive efforts to develop therapeutic strategies targeting either the virus itself or human proteins involved in the infection, resulting in hundreds of potential drugs and numerous clinical trials. In the search for new anti-SARS-CoV-2 drug candidates, we focussed on natural products. Using the cyanobacteria as a source of new structures due to their unique metabolism, two spumigins were isolated from the Baltic cyanobacterium <em>Nodularia spumigena</em> and tested for their activity against SARS-CoV-2. These compounds efficiently reduced SARS-CoV-2 infection in human A549<sup>ACE2/TMPRSS2</sup> cells and fully differentiated primary human airway epithelium cell systems, in which viral entry depends on activation of the viral spike protein by the cellular serine protease, such as TMPRSS2. In contrast, the compounds did not inhibit viral replication in Vero cells, which lack TMPRSS2 but have high levels of cysteine protease cathepsin L, which may serve as an alternative for spike protein activation. Biochemical assays showed that spumigins inhibit TMPRSS2 with an EC<sub>50</sub> ranging from 17 to 85 nM at which no toxicity is observed. Good parameters of identified inhibitors prove that cyanobacteria may serve as a rich source for new scaffolds reaching beyond the canonical chemical combinatorial space.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"615 ","pages":"Article 110763"},"PeriodicalIF":2.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145682668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Forsythoside A inhibits avian infectious bronchitis virus infection by binding the S1 subunit 连翘苷A通过结合S1亚基抑制禽传染性支气管炎病毒感染。

IF 2.4 3区医学

Virology Pub Date : 2026-02-01 Epub Date: 2025-10-22 DOI: 10.1016/j.virol.2025.110715

Ruiting Shen , Jinwei Guo , Xuewei Liu , Peng Yin , Xiaolin Hou

{"title":"Forsythoside A inhibits avian infectious bronchitis virus infection by binding the S1 subunit","authors":"Ruiting Shen , Jinwei Guo , Xuewei Liu , Peng Yin , Xiaolin Hou","doi":"10.1016/j.virol.2025.110715","DOIUrl":"10.1016/j.virol.2025.110715","url":null,"abstract":"<div><h3>Objectives</h3><div>Avian infectious bronchitis virus (IBV) causes acute, highly contagious bronchitis inflammation and severe economic losses in the poultry industry. Such infections are difficult to treat as the virus has high mutation rates. Forsythiaside A (FTA) has an apparent inhibitory effect on IBV. This article describes evidence for the activity and the exact mechanism against IBV.</div></div><div><h3>Methods</h3><div>We observed the inhibitory effect of FTA on IBV infection lifecycle stages using plaque counts, immunofluorescence assay, and RT‒qPCR; then synthesized FTA-OVA, and expressed the recombination protein of IBV spike protein; thirdly, constructed an enzyme-linked immunosorbent assay (ELISA) based on the interaction assay between FTA and IBV particles or IBV spike protein. The interaction between FTA and the S protein was further confirmed using an isothermal titration calorimetry (ITC) system. We predicted the bond modes between FTA and S protein by molecular Docking. Moreover, changed the potential binding amino acids. Then we compared the affinity between FTA and the S1 mutant or the S1 wild-type.</div></div><div><h3>Results</h3><div>FTA inhibited IBV infection, especially on the viral attachment stage at non-cytotoxic concentrations, and FTA bound to virus particles or IBV S1 protein, rather than the S2 subunit. Molecular Docking via Autodock software revealed that FTA could form hydrogen bonds with eight amino acids in the S1 subunit, and these amino acids were then mutated. The affinity between FTA and the S1 mutant was much lower than that of the S1 wild-type.</div></div><div><h3>Conclusion</h3><div>FTA prevents IBV infection by blocking viral absorption and contact with the S1 subunit of IBV but not with S2. We further identified the binding sites by comparing wild-type and mutant S1 affinities.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"614 ","pages":"Article 110715"},"PeriodicalIF":2.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145433532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding cross-serotype interference in duck hepatitis viruses: Trivalent IgY targeting DHAV-1, DHAV-3 and DAstV-1 解码鸭肝炎病毒的跨血清型干扰：靶向DHAV-1、DHAV-3和DAstV-1的三价IgY

IF 2.4 3区医学

Virology Pub Date : 2026-02-01 Epub Date: 2025-12-16 DOI: 10.1016/j.virol.2025.110776

Saisai Zhao , Dongwei Wang , Yafei Qin , Mingtian Mao , Yiman Li , Yizhang Hao , Yaru Fan , Dalin He , Yi Tang , Youxiang Diao

{"title":"Decoding cross-serotype interference in duck hepatitis viruses: Trivalent IgY targeting DHAV-1, DHAV-3 and DAstV-1","authors":"Saisai Zhao , Dongwei Wang , Yafei Qin , Mingtian Mao , Yiman Li , Yizhang Hao , Yaru Fan , Dalin He , Yi Tang , Youxiang Diao","doi":"10.1016/j.virol.2025.110776","DOIUrl":"10.1016/j.virol.2025.110776","url":null,"abstract":"<div><div>To combat the emerging challenge of co-circulating hepatotropic pathogens, duck hepatitis A virus type 1 (DHAV-1), duck hepatitis A virus type 3 (DHAV-3), and Duck astrovirus type 1 (DAstV-1), complicating duck viral hepatitis (DVH) control in China, we engineered a novel trivalent egg yolk immunoglobulin (IgY) preparation designed to transcend serotype-specific limitations and enhance passive immunization efficacy. The polyvalent antibody was produced through multistage immunization protocols in high-yielding laying hens, followed by octanoic acid fractionation purification. The preparation exhibited a potent <em>in vitro</em> neutralization index of up to 1:143 against DHAV-1 and 1:256 against DHAV-3 while demonstrating robust immunoreactivity against the DAstV-1 antibody titer of 1:512 via ELISA. Prophylactic administration conferred complete protection (100 %) in challenge models. Therapeutic intervention yielded survival rates of 70 % for DHAV-1, 90 % for DHAV-3, and 80 % for DAstV-1. To our knowledge, this is the first report of a trivalent IgY agent that concurrently neutralizes the three predominant hepatotropic viruses (DHAV-1, DHAV-3, and DAstV-1) complicating DVH, establishing a broad-spectrum immunoprophylactic and therapeutic strategy.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"615 ","pages":"Article 110776"},"PeriodicalIF":2.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145790501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The roles of sugar metabolism mechanisms and metabolites in viral infections 糖代谢机制和代谢物在病毒感染中的作用。

IF 2.4 3区医学

Virology Pub Date : 2026-02-01 Epub Date: 2025-11-28 DOI: 10.1016/j.virol.2025.110764

Jieyi He , Xianghong Ju , A.M. Abd El-Aty , Xiaoxi Liu , Xiaowen Li

{"title":"The roles of sugar metabolism mechanisms and metabolites in viral infections","authors":"Jieyi He , Xianghong Ju , A.M. Abd El-Aty , Xiaoxi Liu , Xiaowen Li","doi":"10.1016/j.virol.2025.110764","DOIUrl":"10.1016/j.virol.2025.110764","url":null,"abstract":"<div><div>Viral infections pose a persistent challenge to global health by triggering sophisticated molecular interactions and extensive metabolic reprogramming within host organisms. Glycolysis—a central energy metabolism pathway—is dynamically and bidirectionally regulated during infection, reflecting a continuous metabolic interplay between pathogen and host. Rather than maintaining a static metabolic state, glycolytic flux is shaped by competing demands: viruses actively stimulate glycolysis to support replication, while hosts suppress it as a defensive strategy. This regulatory balance shifts across distinct stages of infection—from viral entry and replication peaks to immune clearance—resulting in phase-specific fluctuations in glycolytic activity that reveal a dynamic metabolic tug-of-war. In this review, we synthesize current understanding of how viruses recurrently activate host glycolysis to enhance replication, detailing conserved mechanisms of metabolic hijacking and the multilayered counterstrategies employed by the host. We further evaluate emerging therapeutic approaches, including targeted glycolytic inhibitors and combined immunomodulatory regimens, while addressing challenges related to specificity and efficacy. Finally, we highlight promising research directions such as tissue-specific nanodelivery platforms and single-cell multi-omics integration, which together offer a conceptual framework for developing next-generation antiviral therapies.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"615 ","pages":"Article 110764"},"PeriodicalIF":2.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145663204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological characteristics and genomic study of two Vibrio alginolyticus phages 两种溶藻弧菌噬菌体的生物学特性及基因组学研究。

IF 2.4 3区医学

Virology Pub Date : 2026-02-01 Epub Date: 2025-11-19 DOI: 10.1016/j.virol.2025.110744

Yue Zhang , Yongqi Luo , Chang Liu , Guangfeng Liu , Liling Jiang , Xing Wang , Mengyao Li , Hongsai Zhang , Xiaofang Lai , Jingzhe Jiang

{"title":"Biological characteristics and genomic study of two Vibrio alginolyticus phages","authors":"Yue Zhang , Yongqi Luo , Chang Liu , Guangfeng Liu , Liling Jiang , Xing Wang , Mengyao Li , Hongsai Zhang , Xiaofang Lai , Jingzhe Jiang","doi":"10.1016/j.virol.2025.110744","DOIUrl":"10.1016/j.virol.2025.110744","url":null,"abstract":"<div><div>Vibrio alginolyticus, as a significant pathogen causing highly virulent bacterial diseases in the culture of <em>Babylonia areolata</em>, poses a great challenge to the environment and public health due to the emergence of its drug-resistant strains. In this study, two phages, vB_VapS_BA4 and vB_VapS_BA30, were isolated and identified using <em>V. alginolyticus</em> as the host bacterium. Using techniques such as electron microscopy, biological tests, and genomic analysis, we identified their classification, lytic features, and environmental stability. The results show that phage vB_VapS_BA4 belongs to the genus <em>Vasivirus</em> within the family <em>Inoviridae</em>, with a circular single-stranded DNA (ssDNA) genome. This phage has a latent period ranging from 0 to 20 min and a burst size up to 523.38 PFU/cell, indicating strong antibacterial activity. vB_VapS_BA30 is identified as a new member of the class <em>Caudoviricetes</em>, containing a circular double-stranded DNA (dsDNA) genome with unique genomic features. It has a latent period of less than 10 min and a burst size of 32.97 PFU/cell. Both phages can withstand pH levels from 2 to 12 and temperatures between 4 and 65 °C, showing high environmental adaptability. Additionally, genomic analysis revealed no antibiotic resistance genes or virulence factors, suggesting a safe profile. This study offers a theoretical basis and practical insights for developing innovative phage-based control methods that could reduce antibiotic use, lower the risk of resistance, and support sustainable progress in aquaculture.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"615 ","pages":"Article 110744"},"PeriodicalIF":2.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145663240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0