Virology最新文献_第4页

Synergistic antiviral activity of a cathepsin B/L inhibitor and a TMPRSS2 inhibitor against SARS-CoV-2 in vitro and in vivo 组织蛋白酶B/L抑制剂和TMPRSS2抑制剂体外和体内对SARS-CoV-2的协同抗病毒活性

IF 2.4 3区医学

Virology Pub Date : 2025-08-21 DOI: 10.1016/j.virol.2025.110661

Shinsuke Toba , Kentaro Uemura , Takao Sanaki , Shinji Kusakabe , Kei Konishi , Shigeru Miki , Yuki Maruyama , Atsuhiro Iimuro , Takao Shishido , Michihito Sasaki , Yasuko Orba , William W. Hall , Hirofumi Sawa , Akihiko Sato

{"title":"Synergistic antiviral activity of a cathepsin B/L inhibitor and a TMPRSS2 inhibitor against SARS-CoV-2 in vitro and in vivo","authors":"Shinsuke Toba , Kentaro Uemura , Takao Sanaki , Shinji Kusakabe , Kei Konishi , Shigeru Miki , Yuki Maruyama , Atsuhiro Iimuro , Takao Shishido , Michihito Sasaki , Yasuko Orba , William W. Hall , Hirofumi Sawa , Akihiko Sato","doi":"10.1016/j.virol.2025.110661","DOIUrl":"10.1016/j.virol.2025.110661","url":null,"abstract":"<div><div>The spike (S) protein of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) binds to a host cell receptor which dictates the viral entry pathway. SARS-CoV-2 utilizes two different pathways for cellular entry mediated by both a host type II transmembrane serine protease (TMPRSS2) and cathepsin proteases. These host proteases cleave the viral S protein and initiate membrane fusion allowing viral infection. We previously isolated a SARS-CoV-2 mutant with deletion in the furin cleavage site of the S gene (del2) and revealed differences in cell tropism between wild-type (WT) and del2 viruses. Here, we evaluated the antiviral activities of cellular protease inhibitors against SARS-CoV-2 WT and del2 viruses using several different cell lines. The TMPRSS2 inhibitor, camostat, exhibited strong antiviral activity against WT virus but not del2, while the cathepsin B/L inhibitor, K11777, exhibited potent antiviral activity against the del2 virus. We isolated K11777-escape mutants of SARS-CoV-2 and SARS-CoV and demonstrated that these mutations facilitated S protein cleavage at the S2′ site mediated by cathepsin L. Finally, we demonstrated that combination treatment of K11777 and camostat potently inhibited SARS-CoV-2 WT infection <em>in vitro</em> and <em>in vivo</em>, suggesting the usefulness of combination therapeutics targeting host TMPRSS2 and cathepsin proteases against coronavirus infection. In summary, our study characterized K11777 as an inhibitor of S2′ cleavage by cathepsins, highlighting the critical role of the S2’ site in SARS-CoV-2 cellular entry. This research sheds light on the infection process and has implications for potential therapeutic interventions for SARS-CoV-2 infection.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"612 ","pages":"Article 110661"},"PeriodicalIF":2.4,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the effectiveness and safety of bacteriophage vB_Sen-Miriam1 against Salmonella enterica in in vitro and in vivo models 体外和体内模型评价噬菌体vB_Sen-Miriam1对肠道沙门氏菌的有效性和安全性

IF 2.4 3区医学

Virology Pub Date : 2025-08-20 DOI: 10.1016/j.virol.2025.110660

Miriam Knefel , Monika Zielenkiewicz , Wiktoria Jeleniewska , Łukasz Rąbalski , Małgorzata Kapusta , Marcin Górniak , Wojciech Wesołowski , Kinga Malinowska , Maria Szota , Tomasz Lepionka , Grzegorz Węgrzyn , Alicja Węgrzyn , Łukasz Grabowski

{"title":"Evaluation of the effectiveness and safety of bacteriophage vB_Sen-Miriam1 against Salmonella enterica in in vitro and in vivo models","authors":"Miriam Knefel , Monika Zielenkiewicz , Wiktoria Jeleniewska , Łukasz Rąbalski , Małgorzata Kapusta , Marcin Górniak , Wojciech Wesołowski , Kinga Malinowska , Maria Szota , Tomasz Lepionka , Grzegorz Węgrzyn , Alicja Węgrzyn , Łukasz Grabowski","doi":"10.1016/j.virol.2025.110660","DOIUrl":"10.1016/j.virol.2025.110660","url":null,"abstract":"<div><div>The global rise of antibiotic-resistant <em>Salmonella</em> strains poses a significant public health threat, necessitating the development of alternative antimicrobial strategies. In this study, we present the isolation, characterization, and comprehensive evaluation of the therapeutic potential of a lytic bacteriophage, vB_Sen-Miriam1, targeting <em>Salmonella enterica</em>, using both <em>in vitro</em> and <em>in vivo</em> models. The phage demonstrated a strong lytic activity against multiple <em>Salmonella</em> strains and exhibited therapeutically relevant properties, including a short latent period, high burst size, and stability across a wide range of temperatures and pH values. The phage vB_Sen-Miriam1 possesses a single, circular, double-stranded DNA genome of 43,763 bp with a GC content of 49.9 %. The phage genome analysis confirmed an absence of genes associated with lysogeny, virulence, or antibiotic resistance. vB_Sen-Miriam1 effectively reduced mature biofilm biomass and significantly reduced bacterial counts both extracellularly and intracellularly in the UMNSAH/DF-1 chicken fibroblast infection model. Moreover, in the <em>Galleria mellonella</em> larvae model, the phage treatment increased larval survival while exhibiting no signs of toxicity. These results suggest that vB_Sen-Miriam1 is a promising candidate for further development as a biocontrol agent against <em>Salmonella</em> infections.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"611 ","pages":"Article 110660"},"PeriodicalIF":2.4,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144890851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First complete genome characterisation of an Indian pigeon pox virus directly from a clinical sample 首次直接从临床样本中获得印度鸽痘病毒的完整基因组特征

IF 2.4 3区医学

Virology Pub Date : 2025-08-20 DOI: 10.1016/j.virol.2025.110663

Basanta Pravas Sahu , Subhasmita Panda , Ravi Raj Singh , Subrat Kumar Swain , Niranjan Sahoo , Anjan Kumar Sahoo , Suman Subhangi Sahu , Debasis Nayak

{"title":"First complete genome characterisation of an Indian pigeon pox virus directly from a clinical sample","authors":"Basanta Pravas Sahu , Subhasmita Panda , Ravi Raj Singh , Subrat Kumar Swain , Niranjan Sahoo , Anjan Kumar Sahoo , Suman Subhangi Sahu , Debasis Nayak","doi":"10.1016/j.virol.2025.110663","DOIUrl":"10.1016/j.virol.2025.110663","url":null,"abstract":"<div><div>Avian pox disease is a highly contagious infection caused by the pox virus and has serious consequences for avian species concerning economic and conservation aspects. This viral genus, named <em>Avipoxvirus</em> (APV), infects nearly 300 bird species, and the lack of enough complete genome information hinders inferring this virus's biology. Thus, this study revealed the first complete genome of an Indian pigeonpox virus that belongs to the genus APV, followed by comparative genomics analysis. The entire genome of the present isolate (PPV/Pur-Od-4b/01/Ind) has 280058 bp nucleotide sequences with a GC content of 29.51 %. The unique feature of this complete genome revealed the presence of 270 open reading frames (ORFs) circumscribed by inverted terminal repeats (ITRs) of 4689 bp at each end and the absence of recombination events. The concatenated amino acid phylogenetic tree deciphered the present isolate closely related to the feral pigeonpox virus derived from South Africa. The molecular markers, such as microsatellites, were ubiquitously distributed throughout the genome and were prevalent within the functional genes.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"611 ","pages":"Article 110663"},"PeriodicalIF":2.4,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144895182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-inflammatory agent 35 reduces ASFV replication by suppressing the nuclear translocation of p65 抗炎剂35通过抑制p65的核易位来减少ASFV复制

IF 2.4 3区医学

Virology Pub Date : 2025-08-20 DOI: 10.1016/j.virol.2025.110664

Guanli Dai , Yanlong Zhou , Daming Song , Yiping Cai , Lijiao Yan , Dan Li , Haixue Zheng

{"title":"Anti-inflammatory agent 35 reduces ASFV replication by suppressing the nuclear translocation of p65","authors":"Guanli Dai , Yanlong Zhou , Daming Song , Yiping Cai , Lijiao Yan , Dan Li , Haixue Zheng","doi":"10.1016/j.virol.2025.110664","DOIUrl":"10.1016/j.virol.2025.110664","url":null,"abstract":"<div><div>African swine fever (ASF), caused by the African swine fever virus (ASFV), is a highly contagious infectious disease. To date, no safe and effective vaccine or therapeutic drug is available for ASFV. In this study, we identified Anti-inflammatory agent 35 (A35) as a negative regulator of ASFV replication. Real-time quantitative PCR, hemadsorption assay, and Western blotting analyses revealed that A35 inhibits ASFV genome replication and structural protein expression in <em>vitro</em>. Further studies demonstrated that A35 suppresses TNF-α or ASFV-triggered NF-κB signaling pathway. Reporter assays showed that A35 inhibited p65-triggered NF-κB signaling pathway. Mechanistic studies have found that A35 can inhibit the nuclear translocation of p65. Additionally, overexpression of p65 enhanced ASFV replication, whereas knockdown of p65 had opposite effects. This study reveals, for the first time, that A35 exerts its anti-ASFV effects by targeting NF-κB signal transduction, providing a theoretical basis and candidate compound for the development of novel anti-ASFV agents based on the structural optimization of natural products.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"611 ","pages":"Article 110664"},"PeriodicalIF":2.4,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144886745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zebrafish as a model for SARS-CoV-2 infection, pathogenesis, transmission, and drug screening 斑马鱼作为SARS-CoV-2感染、发病机制、传播和药物筛选的模型

IF 2.4 3区医学

Virology Pub Date : 2025-08-19 DOI: 10.1016/j.virol.2025.110662

Chinnarajan Ravindran , Srestha Mukherjee

引用次数: 0

Immunoinformatics design and experimental expression of a multi-epitope vaccine simultaneously targeting AAV2 and HAdV-F41 against acute hepatitis of unknown etiology 同时靶向AAV2和HAdV-F41治疗不明原因急性肝炎的多表位疫苗的免疫信息学设计和实验表达

IF 2.4 3区医学

Virology Pub Date : 2025-08-18 DOI: 10.1016/j.virol.2025.110653

Fuming Zeng , Qingyang Liu , Xiaorong Wang, Peiluan Zhong, Peihua Wu, Min Yang, Pengcheng Wei

{"title":"Immunoinformatics design and experimental expression of a multi-epitope vaccine simultaneously targeting AAV2 and HAdV-F41 against acute hepatitis of unknown etiology","authors":"Fuming Zeng , Qingyang Liu , Xiaorong Wang, Peiluan Zhong, Peihua Wu, Min Yang, Pengcheng Wei","doi":"10.1016/j.virol.2025.110653","DOIUrl":"10.1016/j.virol.2025.110653","url":null,"abstract":"<div><div>The recent global outbreak of acute hepatitis of unknown etiology (AHUE) in children has raised significant health concerns due to the severity of infections, some of which require liver transplants and can lead to fatalities. Emerging evidence suggests that AHUE is caused by a co-infection involving AAV2 and HAdV-F41. Through immunoinformatics, we identified optimal T-cell and B-cell epitopes from AAV2's VP1 and AAP, as well as from HAdV-F41's long fiber, short fiber, and hexon proteins. To enhance specific immune responses, we incorporated the pan DR-binding epitope (PADRE) and Mycobacterium tuberculosis resuscitation-promoting factor RpfE as adjuvants, linking these elements with appropriate linkers to create a multi-epitope vaccine (MEV). The MEV gene was codon-optimized, cloned into the pET-15b vector, expressed in bacterial hosts, and purified using affinity chromatography. The resulting candidate vaccine, MEV-3, demonstrated high antigenicity, non-allergenicity, and non-toxicity, with a low instability index and favorable molecular characteristics. Molecular dynamics simulations confirmed the vaccine's stable binding to immune receptors, and prokaryotic expression yielded stable and pure MEV-3. Computational immune analysis further predicted a strong immune response induced by this vaccine. In conclusion, we developed an MEV that simultaneously targets AAV2 and HAdV-F41, potentially offering effective prevention and treatment for AHUE.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"611 ","pages":"Article 110653"},"PeriodicalIF":2.4,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144890901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A rapid and robust luciferase-based reporter system to assess SARS-CoV-2 protease activity 基于荧光素酶的快速稳健报告系统评估SARS-CoV-2蛋白酶活性

IF 2.4 3区医学

Virology Pub Date : 2025-08-15 DOI: 10.1016/j.virol.2025.110659

Lucini Paioni Alessandro , Donnici Lorena , Nodari Riccardo , Longo Minnolo Marika , Ferraro Anastasia , Alberico Alessia , Brindisi Margherita , Mejías-Pérez Ernesto , Keppler Oliver T , Summa Vincenzo , Guidotti Luca G , Albanese Manuel , De Francesco Raffaele

{"title":"A rapid and robust luciferase-based reporter system to assess SARS-CoV-2 protease activity","authors":"Lucini Paioni Alessandro , Donnici Lorena , Nodari Riccardo , Longo Minnolo Marika , Ferraro Anastasia , Alberico Alessia , Brindisi Margherita , Mejías-Pérez Ernesto , Keppler Oliver T , Summa Vincenzo , Guidotti Luca G , Albanese Manuel , De Francesco Raffaele","doi":"10.1016/j.virol.2025.110659","DOIUrl":"10.1016/j.virol.2025.110659","url":null,"abstract":"<div><div>Despite effective antiviral drugs that have emerged to combat SARS-CoV-2 infections, novel therapeutic strategies are required to better address the ongoing and future evolutions of the virus. Targeting viral proteases, such as the main protease (Mpro), remains a promising approach. Here, we present a rapid and sensitive luminescence-based reporter system, the i-NSP4/5-Gluc2, to assess Mpro activity. This system employs Gaussia luciferase (Gluc) fused to a pro-interleukin 1β (pro-IL-1β) fragment containing a specific Mpro cleavage site. Upon Mpro cleavage, Gluc is released and secreted, generating a luminescent signal outside the cells. By optimizing the system's design and experimental conditions, we achieved high sensitivity and specificity. The i-NSP4/5-Gluc2 system was validated using the Mpro inhibitor Nirmatrelvir and successfully identified potential Mpro inhibitors from a small library of 46 compounds, as proof of concept. Notably, 13 out of 14 new compounds identified by the i-NSP4/5-Gluc2 assay exhibited potent antiviral activity against live SARS-CoV-2, highlighting the system's accuracy and predictive power. This BSL2-compatible, high-throughput approach facilitates rapid and efficient screening of antiviral compounds, accelerating the development of effective therapeutics against SARS-CoV-2 and future viral pandemics.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"611 ","pages":"Article 110659"},"PeriodicalIF":2.4,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144890865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Murine β-coronavirus MHV-RSA59 orchestrates the interplay of oxidative stress and inflammatory pathways in neuroinflammation 小鼠β冠状病毒MHV-RSA59在神经炎症中协调氧化应激和炎症途径的相互作用

IF 2.4 3区医学

Virology Pub Date : 2025-08-14 DOI: 10.1016/j.virol.2025.110658

Debina Bhattacharyya , Jayasri Das Sarma

{"title":"Murine β-coronavirus MHV-RSA59 orchestrates the interplay of oxidative stress and inflammatory pathways in neuroinflammation","authors":"Debina Bhattacharyya , Jayasri Das Sarma","doi":"10.1016/j.virol.2025.110658","DOIUrl":"10.1016/j.virol.2025.110658","url":null,"abstract":"<div><div>Mouse Hepatitis Virus MHV-A59/RSA59 is hepato-neurotropic strain known to induce an array of stress pathways leading to severe neuroinflammation and demyelination. Previous studies have explored the ability of MHV-A59 or RSA59 to modulate host stress pathways to exert its pathogenic effects. Oxidative and inflammatory stress are critical in neuroinflammation and demyelination, leading to different virus-induced neurodegeneration and associated pathogenesis. Various studies have linked the role of iNOS in the crosstalk of oxidative-inflammatory stress. This study aims to investigate the crosstalk of oxidative stress and inflammatory pathways in M-CoV-MHV-RSA59 infection in a reductionist model in neuronal Neuro2A and astrocytoma DBT cells. Our findings demonstrate that while Neuro2A cells exhibited a persistent increase in ROS levels, leading to upregulation of Nrf2 and its downstream antioxidant enzymes HMOX1 and Catalase, astrocytoma DBT cells displayed a transient ROS peak with an initial downregulation of Nrf2 and impaired antioxidant response. Additionally, we observed differential regulation of key stress responders, such as XBP-1 and DJ-1, highlighting distinct cellular adaptations to viral infection. The inflammatory mediators NF-κB and iNOS activation patterns further underscored the differential inflammatory response, with sustained upregulation in Neuro2A cells, whereas DBT cells showed delayed activation. These findings suggest that neuronal cells engage an intrinsic antioxidant defense, while glial cells exhibit a compromised response. Our study provides mechanistic insights into oxidative and inflammatory crosstalk in the neuro-glial Neuro2A and DBT cells and in MHV-induced neuroinflammation, offering potential therapeutic targets for ameliorating neurodegenerative demyelinating disorders such as multiple sclerosis.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"611 ","pages":"Article 110658"},"PeriodicalIF":2.4,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144858425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The broad-spectrum kayvirus phage disrupts biofilms formed by methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus pseudintermedius 广谱kayvirus噬菌体破坏耐甲氧西林金黄色葡萄球菌和耐甲氧西林假中间葡萄球菌形成的生物膜

IF 2.4 3区医学

Virology Pub Date : 2025-08-14 DOI: 10.1016/j.virol.2025.110657

Sukanya Apiratwarrasakul , Pathomporn Sresuwadjarey , Nathita Phumthanakorn , Patoo Withatanung , Metawee Thongdee , Varintip Lerdsittikul

{"title":"The broad-spectrum kayvirus phage disrupts biofilms formed by methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus pseudintermedius","authors":"Sukanya Apiratwarrasakul , Pathomporn Sresuwadjarey , Nathita Phumthanakorn , Patoo Withatanung , Metawee Thongdee , Varintip Lerdsittikul","doi":"10.1016/j.virol.2025.110657","DOIUrl":"10.1016/j.virol.2025.110657","url":null,"abstract":"<div><div>The escalating global challenge of methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) and <em>Staphylococcus pseudintermedius</em> (MRSP) demands innovative therapeutic approaches. This study comprehensively characterized <em>Staphylococcus</em> phage vB_SauM_VL14 (VL14), a virulent bacteriophage from the <em>Herelleviridae</em> family (<em>Twortvirinae</em> subfamily, <em>Kayvirus</em> genus) with a 141,584-bp linear double-stranded DNA genome. Genomic analysis confirmed that phage VL14 is strictly lytic, containing 224 ORFs and four tRNAs, without lysogenic, virulence, or antimicrobial resistance genes. Phage VL14 demonstrated a 30-min latent period with a burst size of 110 PFU. Host range analysis revealed broad activity, lysing 100 % of <em>S. aureus</em> and 60 % of <em>S. pseudintermedius</em> isolates, including methicillin-resistant strains. The phage achieved significant bacterial reductions and exhibited remarkable biofilm-disrupting capabilities, substantially reducing the biofilm mass and viable cell counts. These findings establish phage VL14 as a broad-spectrum lytic phage with potent bactericidal and biofilm-disrupting activity, supporting its potential as a therapeutic agent against multidrug-resistant <em>Staphylococcus</em> infections and warranting further evaluation in <em>in vivo</em> and clinical settings.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"611 ","pages":"Article 110657"},"PeriodicalIF":2.4,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144864526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maternal antibody transfer efficiency: The impact of M. bovis-BoHV-1 combined vaccine 母源抗体转移效率：牛分枝杆菌- bohv -1联合疫苗的影响

IF 2.4 3区医学

Virology Pub Date : 2025-08-12 DOI: 10.1016/j.virol.2025.110656

Sen Zhang , Guoxing Liu , Aizhen Guo , Yingyu Chen

{"title":"Maternal antibody transfer efficiency: The impact of M. bovis-BoHV-1 combined vaccine","authors":"Sen Zhang , Guoxing Liu , Aizhen Guo , Yingyu Chen","doi":"10.1016/j.virol.2025.110656","DOIUrl":"10.1016/j.virol.2025.110656","url":null,"abstract":"<div><div>Bovine respiratory disease (BRD) is a complex and one of the most costly diseases caused by multiple bacterial and viral infections. Cattle of almost all ages are susceptible to BRD, with young day-old newborn calves being the most at risk. The ability of newborn calves to acquire passive immunity from milk, appears to be a critical issue in immunological evaluation. This study evaluated the maternal antibody transfer capacity of an attenuated and marker <em>M. bovis</em>-BoHV-1 combined vaccine. Results demonstrated that the combined vaccine had a good maternal antibody transfer ability, as evidenced by the detection of high titers of specific antibodies against <em>M. bovis</em> and BoHV-1 in milk of heifers and serum of colostrum-fed newborn calves, with <em>M. bovis</em> antibodies persisting up to 28 days postpartum, peaking at 77.8 % on day 7. Besides, total IgG levels in milk and calf serum were also markedly elevated and consistently different from controls during the first two weeks after birth (<em>p</em> < 0.05). In conclusion, the combined vaccine exhibited favorable maternal antibody transfer ability, and the protection generated by this passive immune response on calves can last for a prolonged period of time, which is essential for improving calf survival.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"611 ","pages":"Article 110656"},"PeriodicalIF":2.4,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144851829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0