Virology最新文献_第4页

The isolation and identification of a novel flavivirus from diseased Chinese soft-shelled turtle (Pelodiscus sinensis) 一种新型黄病毒的分离与鉴定

IF 2.8 3区医学

Virology Pub Date : 2025-04-18 DOI: 10.1016/j.virol.2025.110547

Qi Guo , Sunjian Lyu , Weifeng Shen , Li Liu , Wenjun Ma , Mingxing Zhang , Weishao Bu , Bao Lou

{"title":"The isolation and identification of a novel flavivirus from diseased Chinese soft-shelled turtle (Pelodiscus sinensis)","authors":"Qi Guo , Sunjian Lyu , Weifeng Shen , Li Liu , Wenjun Ma , Mingxing Zhang , Weishao Bu , Bao Lou","doi":"10.1016/j.virol.2025.110547","DOIUrl":"10.1016/j.virol.2025.110547","url":null,"abstract":"<div><div>In recent years, mass mortality of <em>Pelodiscus sinensis</em> has occurred in many Chinese turtle farms and no etiological research on this disease has been conducted to date. The main clinical manifestation of sick <em>P. sinensis</em> was telangiectasis induced bleeding occurred in the limbs, calipash and plastron. After dissection, we found that the blood viscosity was reduced. Typical clinical manifestations included light abdominal cavity effusion, intestine and gastric wall edema and transparency. HE staining showed distinct lesions in liver, intestine, kidney, spleen, heart and lung tissues of infected <em>P. sinensis</em>. TEM observation showed that the spherical virions were approximately 30 nm in diameter. Partial genome of the pathogen was obtained by Illumina sequencing and then assembled and annotated. Phylogenetic analysis of the polyprotein amino acid sequences of this pathogen and other flaviviruses showed that it was closely related to Chinese soft-shelled turtle flavivirus isolate HZ-2017. RT-PCR detection of this virus in the sick <em>P. sinensis</em> from turtle farms showed a high infection rate. QRT-PCR analysis of viral distribution in <em>P. sinensis</em> tissues indicated that the kidney contains the highest amount of virus. The virus was tentatively named “Pelodiscus sinensis flavivirus” (PSFV). In this study, the results of PSFV pathological characteristic and genome information laid a good foundation for further study of this virus.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"608 ","pages":"Article 110547"},"PeriodicalIF":2.8,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143858734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-epitope microsphere vaccine modified immunological efficacy against LMBV in largemouth bass (Micropterus salmoides) 多表位微球疫苗对大口黑鲈LMBV免疫效果的影响

IF 2.8 3区医学

Virology Pub Date : 2025-04-18 DOI: 10.1016/j.virol.2025.110553

Fu-Yi Jiang , Jun-Yao Xia , Yi-Jun Jia , Bin Zhu

{"title":"Multi-epitope microsphere vaccine modified immunological efficacy against LMBV in largemouth bass (Micropterus salmoides)","authors":"Fu-Yi Jiang , Jun-Yao Xia , Yi-Jun Jia , Bin Zhu","doi":"10.1016/j.virol.2025.110553","DOIUrl":"10.1016/j.virol.2025.110553","url":null,"abstract":"<div><div>The largemouth bass <em>iridovirus</em> disease, caused by the largemouth bass virus (LMBV), has a mortality rate of up to 60 %, and no commercial vaccine is available. This study focused on developing a vaccine against LMBV by engineering <em>Escherichia coli</em> to express highly immunogenic antigen epitopes (S11, S12, S3) from preliminary research. A stable recombinant dodecapeptide antigen gene with flexible linkers was constructed, yielding a 25 kDa fusion protein (X1-J) confirmed by SDS-PAGE. The resulting antigen proteins were purified and combined with PLGA (poly (lactic-co-glycolic acid)) to form nanospheres (P-P) with a diameter of 17.2 μm, spherical shape, porous surface, and a 34.13 % drug loading efficiency. By day 21 post-vaccination, both the P groups (X1-J groups) and the P-P groups exhibited significantly elevated immune-related enzyme activities (AKP, CAT, SOD, ACP) in comparison to the controls (<em>P</em> < 0.05). Furthermore, the P-P groups exhibited elevated levels of immune-related gene expression, including <em>IgM</em>, <em>IL-8</em>, <em>TNF-α</em>, <em>IFN-γ</em>, <em>CD40</em>, <em>TGF-β</em>, and <em>IL-10</em>. Finally, we tested the relative percent survival values of the vaccine (P-P) using LMBV, which can reach 15.79 %, 31.58 %, and 47.37 % (5, 10, and 20 μg/g) at 14 days after the injection of the virus. These values were significantly higher than those of the P group at the same concentrations. Our study introduces a new strategy for against LMBV and a reference for developing vaccines using multi-epitope technology.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"608 ","pages":"Article 110553"},"PeriodicalIF":2.8,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The GI-19 dominant genotype of infectious bronchitis virus in chickens from 2021 to 2023 in Sichuan province is frequently involved in recombination events 2021 - 2023年四川省鸡传染性支气管炎病毒GI-19显性基因型频繁参与重组事件

IF 2.8 3区医学

Virology Pub Date : 2025-04-18 DOI: 10.1016/j.virol.2025.110543

Fuyan Wang , Wenjun Yan , Lijia Liu , Dongli Shu , Xin Yang , Wenlong Xu

{"title":"The GI-19 dominant genotype of infectious bronchitis virus in chickens from 2021 to 2023 in Sichuan province is frequently involved in recombination events","authors":"Fuyan Wang , Wenjun Yan , Lijia Liu , Dongli Shu , Xin Yang , Wenlong Xu","doi":"10.1016/j.virol.2025.110543","DOIUrl":"10.1016/j.virol.2025.110543","url":null,"abstract":"<div><div>Infectious bronchitis virus (IBV), the etiological agent of infectious bronchitis (IB) in chickens, is a highly contagious respiratory disease that poses significant economic burdens on the global poultry industry. Comprehensive knowledge of the epidemiological patterns and genetic variations of IBV is crucial for effective prevention and control strategies. In this study, we collected 684 suspected samples from Sichuan province between 2021 and 2023. PCR testing revealed a total positivity rate of 26.9 %, with the Guangyuan region exhibiting the highest positivity rate at 37.2 %. Subsequently, we obtained 21 complete IBV S1 gene sequences and the phylogenetic analysis identified the GI-19 type as the predominant strain. Comparing nucleic acid similarity among the 21 isolated strains, we observed a range of 66.48 %–99.69 % nucleotide similarity (56.22 %–99.45 % in amino acids). The QXL87 vaccine strain exhibited higher similarity to the isolated strains. Amino acid variations in the three hypervariable regions (HVRs) showed the highest variability in HVR I. The GVI type strain differed in amino acid composition from QXL87 in HVR I, resulting in reduced N-glycosylation sites on the S1 gene. Furthermore, all isolated strains displayed varying reductions in N-glycosylation sites on the S1 gene compared to the QXL87 vaccine strain. Ultimately, recombination analysis revealed frequent involvement of the GI-19 and GI-22 strains in gene recombination. The majority of recombined strains were derived from partial segments of the GI-19 strain, with no recombination observed in any of the isolated GI-19 strains. In summary, our findings elucidate the prevalence of IBV in Sichuan province and highlight the pivotal role of the GI-19 strain in IBV recombination, thereby offering valuable data support for IBV control.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"608 ","pages":"Article 110543"},"PeriodicalIF":2.8,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143873325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cross-protection against St. Louis encephalitis virus and Usutu virus by West Nile virus convalescent plasma 西尼罗病毒恢复期血浆对圣路易斯脑炎病毒与Usutu病毒的交叉保护作用

IF 2.8 3区医学

Virology Pub Date : 2025-04-18 DOI: 10.1016/j.virol.2025.110555

Md Shakhawat Hossain, Megan B. Vogt, Seth A. Hawks, Sheryl L. Coutermarsh-Ott, Nisha K. Duggal

{"title":"Cross-protection against St. Louis encephalitis virus and Usutu virus by West Nile virus convalescent plasma","authors":"Md Shakhawat Hossain, Megan B. Vogt, Seth A. Hawks, Sheryl L. Coutermarsh-Ott, Nisha K. Duggal","doi":"10.1016/j.virol.2025.110555","DOIUrl":"10.1016/j.virol.2025.110555","url":null,"abstract":"<div><div>West Nile virus (WNV), Usutu virus (USUV), and Saint Louis encephalitis virus (SLEV) are emerging mosquito-borne flaviviruses within the Japanese encephalitis virus serocomplex. They share a similar transmission cycle between passerine birds and <em>Culex</em> spp. mosquitoes, and their spillover can cause neuroinvasive diseases among humans and animals. Individuals might be exposed to more than one of these viruses during their lifetime. Previously, we found that WNV vaccination protected mice from USUV disease, and sera collected from the WNV vaccinated mice cross-neutralized USUV <em>in vitro.</em> However, whether WNV convalescent (post-recovery) plasma cross-protects against heterologous SLEV or USUV infection is unknown. In this study, <em>in vivo</em> experiments were conducted to assess whether WNV human convalescent plasma and/or mouse convalescent serum afforded protection to mice against SLEV and USUV infection and neuroinvasion. First, we found that human and mouse WNV convalescent samples cross-neutralized USUV and SLEV <em>in vitro</em>. We then passively transferred human or mouse WNV convalescent samples into mice and challenged them with WNV, USUV, or SLEV. Both human and mouse WNV convalescent samples reduced WNV neuroinvasion and SLEV and USUV viremia during acute infection. Mouse WNV convalescent serum significantly reduced SLEV titers in the brain and showed a trend towards resulting in less inflammation in the brain. These findings helped to better understand the potential cross-protection among WNV, SLEV, and USUV, and identified cross-neutralizing antibodies as potential correlates of protection for individuals exposed to multiple flaviviruses, though protection was incomplete.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"608 ","pages":"Article 110555"},"PeriodicalIF":2.8,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the pathogenic and transmission characteristics of JN.1 in golden hamsters based on different attack methods 基于不同的攻毒方法，探讨JN.1在金仓鼠中的致病及传播特点

IF 2.8 3区医学

Virology Pub Date : 2025-04-17 DOI: 10.1016/j.virol.2025.110548

Ruixue Liu , Fang Song , Li Sun , Fang Yan , Qiushi Jin , Fang Yan , Xianzhu Xia , Xuefeng Wang , Yuwei Gao

{"title":"Exploring the pathogenic and transmission characteristics of JN.1 in golden hamsters based on different attack methods","authors":"Ruixue Liu , Fang Song , Li Sun , Fang Yan , Qiushi Jin , Fang Yan , Xianzhu Xia , Xuefeng Wang , Yuwei Gao","doi":"10.1016/j.virol.2025.110548","DOIUrl":"10.1016/j.virol.2025.110548","url":null,"abstract":"<div><div>Since its emergence at the end of 2023, the JN.1 variant of COVID-19 has become the dominant strain globally. Currently, its characteristics in related animal models remain largely unknown. The results indicate that JN.1 can cause weight loss, viral load, viral titer, and histopathological changes in golden hamsters via intranasal and intragastric inoculation methods, with intranasal inoculation leading to faster viral replication. Interestingly, both viral load and viral titer of JN.1 are significantly lower than those of its parental strains BA.2 and XBB EG.5.1. A comparison of hematological data from the two inoculation methods was also consistent with previous findings. This highlights the importance of the infection route in studying the virus's progression and characteristics. In direct transmission studies of JN.1, the minimum time for virus transmission was 24 h, while XBB EG.5.1 could transmit the virus in as little as 6 h. Finally, the in vivo adaptability of JN.1 was investigated, with XBB EG.5.1 showing a more apparent adaptability advantage. Therefore, compared with EG.5.1, the pathogenicity and transmissibility of JN.1 are significantly weakened.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"608 ","pages":"Article 110548"},"PeriodicalIF":2.8,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment and optimization of a rapid and convenient viral RNA transcript copy reduction neutralization test (VcRNT) for quantification of hantaan orthohantavirus (HTNV) neutralizing antibodies 汉滩正汉坦病毒（HTNV）中和抗体定量检测方法的建立与优化

IF 2.8 3区医学

Virology Pub Date : 2025-04-17 DOI: 10.1016/j.virol.2025.110542

Qiqi Yang , Jing Wei , Chuantao Ye , Jiawei Pei , Xuemin Pei , Yuan Wang , Yangchao Dong , Hui Zhang , Dongshen Jiang , Xiaojing Yang , Hongwei Ma , Linfeng Cheng , He Liu , Liang Zhang , Yingfeng Lei , Zhikai Xu , Pengbo Yu , Fanglin Zhang , Wei Ye

{"title":"Establishment and optimization of a rapid and convenient viral RNA transcript copy reduction neutralization test (VcRNT) for quantification of hantaan orthohantavirus (HTNV) neutralizing antibodies","authors":"Qiqi Yang , Jing Wei , Chuantao Ye , Jiawei Pei , Xuemin Pei , Yuan Wang , Yangchao Dong , Hui Zhang , Dongshen Jiang , Xiaojing Yang , Hongwei Ma , Linfeng Cheng , He Liu , Liang Zhang , Yingfeng Lei , Zhikai Xu , Pengbo Yu , Fanglin Zhang , Wei Ye","doi":"10.1016/j.virol.2025.110542","DOIUrl":"10.1016/j.virol.2025.110542","url":null,"abstract":"<div><div>Rodent-borne orthohantavirus causes severe hemorrhagic fever worldwide, with hemorrhagic fever with renal syndrome (HFRS) in Eurasia, and hantavirus cardiopulmonary syndrome (HCPS) in the Amrerica. In East Asia, Hantaan orthohantavirus (HTNV) is the main pathogen responsible for severe HFRS, with a case fatality rate up to 10 % with no specific treatment available. The antisera or neutralizing antibody (NAb) is able to block virus infection, however, the traditional NAb titer measuring based on focus reduction neutralization test (FRNT) is quite labour-extensive and takes 7–10 days. This study aims to shorten the measuring time of NAb neutralization efficiency by 1–2 days based on quantitative RT-PCR. For this purpose, we developed an <em>in vitro</em> transcripted viral RNA standard and generated a viral RNA copy number standard curve. Using this standard curve, we compared the HTNV propagation kinetics between viral RNA copy numbers and secreted infectious virion. The detection limit and suitable timeframe and condition for qRT-PCR based viral RNA copy numbers measuring was also determined. In addition, when applying this method to measuring the NAb neutralization efficiency of HFRS convalescent serum samples, we could obtain the NAb neutralization efficiency within 1 or 2 days. Furthermore, this method was also nicely correlated with the FRNT - based NAb measurement. To conclude, we established a rapid and convenient viral RNA transcripts copy reduction neutralization test (VcRNT) to measure NAb neutralization efficiency that could finish within 1 or 2 days, and provided a reliable and efficient alternative for FRNT.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"608 ","pages":"Article 110542"},"PeriodicalIF":2.8,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143854944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A tomato mottle mosaic virus-based vector system for gene function studies in tomato 基于番茄斑驳花叶病毒的番茄基因功能研究载体系统

IF 2.8 3区医学

Virology Pub Date : 2025-04-16 DOI: 10.1016/j.virol.2025.110549

Zhi-Yong Yan , Carlos Kwesi Tettey , Hua-Yu Ma , Xiu-Qi Mu , Jun Jiang , Chao Geng , Yan-ping Tian , Xiao Yin , Xiang-Dong Li

{"title":"A tomato mottle mosaic virus-based vector system for gene function studies in tomato","authors":"Zhi-Yong Yan , Carlos Kwesi Tettey , Hua-Yu Ma , Xiu-Qi Mu , Jun Jiang , Chao Geng , Yan-ping Tian , Xiao Yin , Xiang-Dong Li","doi":"10.1016/j.virol.2025.110549","DOIUrl":"10.1016/j.virol.2025.110549","url":null,"abstract":"<div><div>Plant virus-based vectors provide a convenient tool for rapid protein expression and gene function studies. However, there is little research on viral vectors capable of systemically expressing proteins in tomatoes. Here, we substituted the coat protein (<em>CP</em>) gene with <em>GFP</em> gene in a previously constructed tomato mottle mosaic virus (ToMMV; genus <em>Tobamovirus</em>) infectious clone pCBToMMV to produce transiently expressing vectors, which could express <em>GFP</em> in the infiltrated leaves of <em>Nicotiana benthamiana</em> plants. We then inserted the sub-genomic promoter and <em>CP</em> gene from another tomamovirus tomato mosaic virus downstream of <em>GFP</em> gene to form a vector capable of systemically expressing <em>GFP</em> in both <em>N. benthamiana</em> and <em>Solanum lycopersicum</em> plants. The ToMMV-based vector also expressed the MYB-related transcription factor Rosea1, inducing anthocyanin biosynthesis in the systemic leaves of both <em>N. benthamiana</em> and <em>S. lycopersicum</em> plants. Additionally, expressing the <em>bialaphos resistance</em> gene using the ToMMV vector conferred resistance to the herbicide glufosinate-ammonium in plants. Furthermore, the ToMMV vector successfully expressed a 68 kDa β-glucuronidase in systemic leaves and roots of <em>N. benthamiana</em> and <em>S. lycopersicum</em> plants. This ToMMV-based vector provides a simple and efficient tool for gene function studies in tomatoes.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"608 ","pages":"Article 110549"},"PeriodicalIF":2.8,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding EBV infection and EBV-associated lymphomas in children 了解儿童eb病毒感染和eb病毒相关淋巴瘤

IF 2.8 3区医学

Virology Pub Date : 2025-04-16 DOI: 10.1016/j.virol.2025.110544

Chabay P

引用次数: 0

The evolving landscape of Chandipura virus: A comprehensive account of outbreaks to recent advances 昌迪普拉病毒的演变图景：对疫情爆发的全面描述和最新进展

IF 2.8 3区医学

Virology Pub Date : 2025-04-15 DOI: 10.1016/j.virol.2025.110541

Disharee Mallick , Urvashi Yadav , Megha Gupta , Dilip Kumar , Rajesh Kumar

{"title":"The evolving landscape of Chandipura virus: A comprehensive account of outbreaks to recent advances","authors":"Disharee Mallick , Urvashi Yadav , Megha Gupta , Dilip Kumar , Rajesh Kumar","doi":"10.1016/j.virol.2025.110541","DOIUrl":"10.1016/j.virol.2025.110541","url":null,"abstract":"<div><div>Chandipura virus (CHPV), a member of the family <em>Rhabdoviridae</em>, has garnered attention due to its significant implications on human health, particularly in India, where it has contributed to encephalitis outbreaks. This review provides a full-spectrum analysis of CHPV, detailing its origin, historical context, and geographical distribution, which primarily spans India and parts of Africa. CHPV is predominantly transmitted through infected sandflies, although alternative transmission routes cannot be overruled. Neurotropism plays a vital role in CHPV-associated pathogenesis, leading to severe neurological ailments, including encephalitis and fatalities among children at a significantly high rate. Current diagnostic approaches for CHPV infection harness molecular biology tools like PCR for viral RNA detection and serological methods to identify antibodies. Effective therapeutic strategies remain limited, with antivirals such as Favipiravir indicating strong prospects in preclinical studies. We also discuss various animal models used in CHPV research, including murine models, offering critical insights into the CHPV pathogenesis and evaluating the efficacy of potential therapeutic interventions. Concisely, this review underscores the significance of robust monitoring and further research to enhance our understanding of CHPV and develop effective strategies for its control and prevention.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"608 ","pages":"Article 110541"},"PeriodicalIF":2.8,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A phycobiliprotein-based reporter assay for the evaluation of SARS-CoV-2 main protease activity 基于藻胆蛋白的报告试验评估SARS-CoV-2主要蛋白酶活性

IF 2.8 3区医学

Virology Pub Date : 2025-04-12 DOI: 10.1016/j.virol.2025.110540

Le Cao , Shuyuan Shi , Chaofeng Zhang , Cheng Zhao

{"title":"A phycobiliprotein-based reporter assay for the evaluation of SARS-CoV-2 main protease activity","authors":"Le Cao , Shuyuan Shi , Chaofeng Zhang , Cheng Zhao","doi":"10.1016/j.virol.2025.110540","DOIUrl":"10.1016/j.virol.2025.110540","url":null,"abstract":"<div><div>SARS-CoV-2 M<sup>pro</sup> is crucial for viral replication and transcription and is highly conserved. Therefore, it is an ideal target for developing broad-spectrum antiviral drugs. To address resistance to existing drugs caused by mutations, a simple and sensitive method for detecting the activity of M<sup>pro</sup> is needed. Considering the excellent fluorescence properties of phycobiliproteins, this study developed a phycobiliprotein-based reporter assay to evaluate M<sup>pro</sup> activity. An engineered lyase was generated by inserting the M<sup>pro</sup> recognition sequence between the phycobiliprotein lyases CpcF and CpcE. To ensure that the binding of CpcE and CpcF depended on the linker, a series of truncated forms were constructed. Among them, the activity of CpcE/F-10 was significantly reduced in the presence of M<sup>pro</sup>; however, both genetic and chemical inhibition of M<sup>pro</sup> activity reversed these results. These data indicated that the fluorescence of phycobiliproteins was negatively correlated with M<sup>pro</sup> activity. The reporter assay developed here will contribute to determining the impact of M<sup>pro</sup> mutations and screening for new inhibitors.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"608 ","pages":"Article 110540"},"PeriodicalIF":2.8,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143826225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0