First complete genome characterisation of an Indian pigeon pox virus directly from a clinical sample

IF 2.4 3区医学 Q3 VIROLOGY

Virology Pub Date : 2025-08-20 DOI:10.1016/j.virol.2025.110663

Basanta Pravas Sahu , Subhasmita Panda , Ravi Raj Singh , Subrat Kumar Swain , Niranjan Sahoo , Anjan Kumar Sahoo , Suman Subhangi Sahu , Debasis Nayak

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引用次数: 0

Abstract

Avian pox disease is a highly contagious infection caused by the pox virus and has serious consequences for avian species concerning economic and conservation aspects. This viral genus, named Avipoxvirus (APV), infects nearly 300 bird species, and the lack of enough complete genome information hinders inferring this virus's biology. Thus, this study revealed the first complete genome of an Indian pigeonpox virus that belongs to the genus APV, followed by comparative genomics analysis. The entire genome of the present isolate (PPV/Pur-Od-4b/01/Ind) has 280058 bp nucleotide sequences with a GC content of 29.51 %. The unique feature of this complete genome revealed the presence of 270 open reading frames (ORFs) circumscribed by inverted terminal repeats (ITRs) of 4689 bp at each end and the absence of recombination events. The concatenated amino acid phylogenetic tree deciphered the present isolate closely related to the feral pigeonpox virus derived from South Africa. The molecular markers, such as microsatellites, were ubiquitously distributed throughout the genome and were prevalent within the functional genes.

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首次直接从临床样本中获得印度鸽痘病毒的完整基因组特征

禽痘病是由禽痘病毒引起的一种高度传染性疾病，在经济和保护方面对禽类物种造成严重后果。这种病毒属被命名为Avipoxvirus (APV)，感染近300种鸟类，缺乏足够的完整基因组信息阻碍了对这种病毒生物学的推断。因此，本研究揭示了属于APV属的印度鸽痘病毒的第一个完整基因组，随后进行了比较基因组学分析。该分离物（PPV/Pur-Od-4b/01/Ind）全基因组全长280058 bp， GC含量为29.51%。该完整基因组的独特特征显示，存在270个开放阅读框（orf），每端有4689 bp的反向末端重复序列（itr），并且没有重组事件。连接的氨基酸系统发育树破译了目前与南非野鸽痘病毒密切相关的分离物。微卫星等分子标记在整个基因组中普遍存在，并普遍存在于功能基因中。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Virology 医学-病毒学

CiteScore

6.00

自引率

0.00%

发文量

157

审稿时长

50 days

期刊介绍： Launched in 1955, Virology is a broad and inclusive journal that welcomes submissions on all aspects of virology including plant, animal, microbial and human viruses. The journal publishes basic research as well as pre-clinical and clinical studies of vaccines, anti-viral drugs and their development, anti-viral therapies, and computational studies of virus infections. Any submission that is of broad interest to the community of virologists/vaccinologists and reporting scientifically accurate and valuable research will be considered for publication, including negative findings and multidisciplinary work.Virology is open to reviews, research manuscripts, short communication, registered reports as well as follow-up manuscripts.