广谱kayvirus噬菌体破坏耐甲氧西林金黄色葡萄球菌和耐甲氧西林假中间葡萄球菌形成的生物膜

IF 2.4 3区医学 Q3 VIROLOGY

Virology Pub Date : 2025-08-14 DOI:10.1016/j.virol.2025.110657

Sukanya Apiratwarrasakul , Pathomporn Sresuwadjarey , Nathita Phumthanakorn , Patoo Withatanung , Metawee Thongdee , Varintip Lerdsittikul

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引用次数: 0

摘要

耐甲氧西林金黄色葡萄球菌（MRSA）和假中间葡萄球菌（MRSP）的全球挑战不断升级，需要创新的治疗方法。本研究全面表征了一种来自Herelleviridae家族（twortvirae亚科，Kayvirus属）的强毒噬菌体——葡萄球菌噬菌体vB_SauM_VL14 (VL14)，其线性双链DNA基因组长度为141584 bp。基因组分析证实噬菌体VL14具有严格的裂解性，含有224个orf和4个trna，不含溶原性、毒力性或耐药基因。噬菌体VL14潜伏期为30分钟，爆发大小为110 PFU。宿主范围分析显示具有广泛的活性，可裂解100%的金黄色葡萄球菌和60%的假中间葡萄球菌分离株，包括耐甲氧西林菌株。噬菌体实现了显著的细菌减少，并表现出显著的生物膜破坏能力，大大减少了生物膜质量和活细胞计数。这些发现证实了噬菌体VL14是一种广谱裂解噬菌体，具有强大的杀菌和生物膜破坏活性，支持其作为治疗多重耐药葡萄球菌感染的潜力，并需要在体内和临床环境中进一步评估。

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The broad-spectrum kayvirus phage disrupts biofilms formed by methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus pseudintermedius

The escalating global challenge of methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus pseudintermedius (MRSP) demands innovative therapeutic approaches. This study comprehensively characterized Staphylococcus phage vB_SauM_VL14 (VL14), a virulent bacteriophage from the Herelleviridae family (Twortvirinae subfamily, Kayvirus genus) with a 141,584-bp linear double-stranded DNA genome. Genomic analysis confirmed that phage VL14 is strictly lytic, containing 224 ORFs and four tRNAs, without lysogenic, virulence, or antimicrobial resistance genes. Phage VL14 demonstrated a 30-min latent period with a burst size of 110 PFU. Host range analysis revealed broad activity, lysing 100 % of S. aureus and 60 % of S. pseudintermedius isolates, including methicillin-resistant strains. The phage achieved significant bacterial reductions and exhibited remarkable biofilm-disrupting capabilities, substantially reducing the biofilm mass and viable cell counts. These findings establish phage VL14 as a broad-spectrum lytic phage with potent bactericidal and biofilm-disrupting activity, supporting its potential as a therapeutic agent against multidrug-resistant Staphylococcus infections and warranting further evaluation in in vivo and clinical settings.

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来源期刊

Virology 医学-病毒学

CiteScore

6.00

自引率

0.00%

发文量

157

审稿时长

50 days

期刊介绍： Launched in 1955, Virology is a broad and inclusive journal that welcomes submissions on all aspects of virology including plant, animal, microbial and human viruses. The journal publishes basic research as well as pre-clinical and clinical studies of vaccines, anti-viral drugs and their development, anti-viral therapies, and computational studies of virus infections. Any submission that is of broad interest to the community of virologists/vaccinologists and reporting scientifically accurate and valuable research will be considered for publication, including negative findings and multidisciplinary work.Virology is open to reviews, research manuscripts, short communication, registered reports as well as follow-up manuscripts.