Randolph M Baral, Bente Flatland, Susan M Jaensch, Douglas A Lessels, Kathleen P Freeman
{"title":"Regression Using 20 Samples to Harmonize a Point-of-Care Analyzer With a Commercial Laboratory Analyzer for Feline Plasma Biochemistry Testing.","authors":"Randolph M Baral, Bente Flatland, Susan M Jaensch, Douglas A Lessels, Kathleen P Freeman","doi":"10.1111/vcp.70108","DOIUrl":"https://doi.org/10.1111/vcp.70108","url":null,"abstract":"<p><strong>Background: </strong>Harmonization enables direct comparison of patient results from different analyzers and/or measurement techniques and the use of common reference intervals and decision thresholds. Point-of-care analyzers (POC) cannot be calibrated by the end user, so regression offers an opportunity for calculated harmonization. This approach has been used to harmonize (POC) with commercial laboratory analyzers (CL) with > 90 paired feline plasma samples.</p><p><strong>Objective: </strong>Our aims were to use 20 pairs of results to derive regression equations to harmonize the POC results with those of the CL. Harmonized results were assessed by recognized quality goals.</p><p><strong>Methods: </strong>Systematic sampling was used to choose the 20 samples used to calculate regression parameters (with three techniques). These were applied to the remainder of the original dataset to harmonize the POC results to those from the CL. POC results were assessed for bias and the number of results reaching quality goals before and after adjustment.</p><p><strong>Results: </strong>Regression from 20 paired samples was able to harmonize POC analyzer results to a commercial laboratory analyzer for 16 feline plasma biochemistry measurands.</p><p><strong>Conclusions: </strong>Most analytes could be harmonized by this approach. Reasons for harmonization failure were inadequate concentration range of patient results (AST, lipase, total bilirubin, GGT), potential non-linear relationship (lipase), and no clinical performance goal (globulins). Additional studies using samples with clinically important values may overcome these limitations, and similar studies of other species and other types of point-of-care analyzers would be of interest.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147692521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sarena M Krojanker, Maria Jaramillo, Brad D Cingolani, Floyd L Williams, Priscila B S Serpa, Andrew D Woolcock
{"title":"Codocytosis in the Dog: 345 Cases (2020-2022).","authors":"Sarena M Krojanker, Maria Jaramillo, Brad D Cingolani, Floyd L Williams, Priscila B S Serpa, Andrew D Woolcock","doi":"10.1111/vcp.70107","DOIUrl":"https://doi.org/10.1111/vcp.70107","url":null,"abstract":"<p><strong>Introduction: </strong>Codocytes, or target cells, are a morphologic variation of erythrocytes characterized by increased membrane surface area relative to volume. In dogs, codocytosis is frequently noted on blood smear evaluation, but its clinical significance remains poorly understood.</p><p><strong>Objectives: </strong>To characterize the clinical conditions associated with codocytosis in dogs and assess relationships between codocyte severity and hematologic, biochemical, and diagnostic findings.</p><p><strong>Methods: </strong>A retrospective descriptive study was conducted on 345 dogs from a veterinary teaching hospital from September 2020 through October 2022. Dogs were included if their CBC report described codocytosis graded as ≥ 2+. Data collected included CBC results, serum biochemistry, clinical diagnoses, and current medications. Codocytosis was graded semi-quantitatively (2+ to 4+). Statistical comparisons were made using chi-square tests.</p><p><strong>Results: </strong>Codocytosis ≥ 2+ was identified in 2.5% of 14 010 canine CBCs during the study. The majority (81.4%) had 2+ codocytes. There were no significant differences in anemia, macrocytosis, or regeneration across grades. Dogs with 3+ or 4+ codocytosis had significantly higher alanine transferase (ALT) activity and were more frequently diagnosed with hepatobiliary (p = 0.006) and gastrointestinal (p = 0.009) diseases. Endocrine disease, particularly diabetes mellitus was also frequently observed. Antibiotic use was significantly associated with higher codocyte grades (p < 0.001), though no causation was identified.</p><p><strong>Conclusions: </strong>Codocytosis in dogs is not primarily associated with anemia or regeneration, but rather appears more frequently in diseases affecting hepatobiliary, gastrointestinal, and endocrine systems. These findings suggest a multifactorial pathogenesis likely involving altered lipid metabolism and membrane remodeling. Recognition of codocytosis may provide insight into systemic disease processes in canine.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147692444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nathkapach K Rattanapitoon, Natthawut Charoenphon, Patpicha Arunsan, Schawanya K Rattanapitoon
{"title":"Letter to the Editor Re: Unusual Electrophoretic Pattern in a Dog Infected With Angiostrongylus vasorum.","authors":"Nathkapach K Rattanapitoon, Natthawut Charoenphon, Patpicha Arunsan, Schawanya K Rattanapitoon","doi":"10.1111/vcp.70082","DOIUrl":"https://doi.org/10.1111/vcp.70082","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147370188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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