Veterinary clinical pathology最新文献

{"title":"What is your diagnosis? Cerebrospinal fluid from a Labrador Retriever","authors":"Sidney N. Bogue, Joan R. Coates, Jimmy C. Lattimer, Angela B. Royal","doi":"10.1111/vcp.13403","DOIUrl":"10.1111/vcp.13403","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 S1","pages":"S19-S21"},"PeriodicalIF":1.1,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142819451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryoglobulinemia concurrent with Leishmania infantum infection in a dog and its interference with two automated hematology analyzers","authors":"Javier Martínez-Caro,&nbsp;Beatriz Agulla,&nbsp;Alba Benet,&nbsp;Josep Pastor","doi":"10.1111/vcp.13398","DOIUrl":"10.1111/vcp.13398","url":null,"abstract":"<p>A 6-year-old, intact male English Bulldog presented for evaluation of weakness. Physical examination revealed mildly pale mucous membranes and ophthalmologic alterations. A complete blood cell count was performed on the Urit Smart V5 and the Sysmex XN-1000V, and showed several hematologic discrepancies between the analyzers. The Urit reported marked leukocytosis and thrombocytosis, and the Sysmex reported marked thrombocytosis on the impedance and optical RET channel but mild thrombocytopenia on the optical PLT-F channel. On the blood smear examination, there was an abundant proteinaceous material compatible with cryoglobulins. The red blood cell exhibited a notable degree of poikilocytosis. The number of leukocytes was found to be correlated with the Sysmex total white blood cell count, while the platelet count demonstrated a better correlation with the optical PLT-F channel from the Sysmex. Centrifugation of the blood in a capillary tube (at room temperature) identified a cryoglobulin precipitate. Additionally, the blood was reanalyzed soon after warming it to 37°C, which partially corrected most of the interferences. The serum protein electrophoresis demonstrated a marked hyperproteinemia with mild hypoalbuminemia and a marked hypergammaglobulinemia. On diagnostic imaging, a mild splenomegaly was noted. Cytology of the spleen showed <i>Leishmania infantum</i> infection, plasma cell hyperplasia, and extramedullary hematopoiesis. This is the first description of a dog infected with <i>Leishmania</i>. <i>infantum</i> with concurrent monoclonal hypergammaglobulinemia and cryoglobulinemia. We present a detailed description of the interference of cryoglobulin with the Urit Smart V5 and the Sysmex XN-1000V, along with the usefulness of Sysmex PLT-F in this condition.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 1","pages":"15-23"},"PeriodicalIF":1.2,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.13398","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142814325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of criteria to optimize manual smear review of automated complete blood counts using a machine learning model.","authors":"Jennifer M Hayes, Mitchell R Hayes, Kristen R Friedrichs, Heather A Simmons","doi":"10.1111/vcp.13400","DOIUrl":"10.1111/vcp.13400","url":null,"abstract":"<p><strong>Objective: </strong>In this study, we aim to determine if machine learning can reduce manual smear review (MSR) rates while meeting or exceeding the performance of traditional MSR criteria.</p><p><strong>Method: </strong>9938 automated CBCs with paired MSRs were performed on samples from rhesus and cynomolgus macaques. The definition of a positive (abnormal) smear was determined. Two expert-derived MSR criteria were created: criteria adapted from published, standardized human laboratory criteria (Adapted International Consensus Guidelines[aICG]) and internally generated criteria (Center Consensus Guidelines [CCG]). An ensemble machine learning model was trained on an independent subset of the data to optimize the balanced accuracy of classification, a combined measure of sensitivity and specificity. The resulting machine learning model and the two expert-derived MSR criteria were applied to a test dataset, and their performance compared.</p><p><strong>Results: </strong>aICG criteria demonstrated high sensitivity (80.8%) and MSR rate (74.2%) while CCG criteria demonstrated lower sensitivity (57.1%) and MSR rate (36.1%). The machine learning model integrated with CCG criteria had a superior combination of both sensitivity (76.8%) and MSR rate (45.1%) achieving a false negative rate of 1.6%.</p><p><strong>Conclusion: </strong>Machine learning in combination with expert-derived criteria can optimize the selection of samples for MSR thus decreasing MSR rates and labor efforts required for CBC performance.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12137682/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing artificial intelligence for enhanced veterinary diagnostics: A look to quality assurance, Part I Model development.","authors":"Christina Pacholec, Bente Flatland, Hehuang Xie, Kurt Zimmerman","doi":"10.1111/vcp.13401","DOIUrl":"https://doi.org/10.1111/vcp.13401","url":null,"abstract":"<p><p>Artificial intelligence (AI) has transformative potential in veterinary pathology in tasks ranging from cell enumeration and cancer detection to prognosis forecasting, virtual staining techniques, and individually tailored treatment plans. Preclinical testing and validation of AI systems (AIS) are critical to ensure diagnostic safety, efficacy, and dependability. In this two-part series, challenges such as the AI chasm (ie, the discrepancy between the AIS model performance in research settings and real-world applications) and ethical considerations (data privacy, algorithmic bias) are reviewed and underscore the importance of tailored quality assurance measures that address the nuances of AI in veterinary pathology. This review advocates for a multidisciplinary approach to AI development and implementation, focusing on image-based tasks, highlighting the necessity for collaboration across veterinarians, computer scientists, and ethicists to successfully navigate the complex landscape of using AI in veterinary medicine. It calls for a concerted effort to bridge the AI chasm by addressing technical, ethical, and regulatory challenges, facilitating AI integration into veterinary pathology. The future of veterinary pathology must balance harnessing AI's potential while intentionally mitigating its risks, ensuring the welfare of animals and the integrity of the veterinary profession are safeguarded. Part I of this review focuses on considerations for model development, and Part II focuses on external validation of AI.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A clinical and hematologic approach to basophilia in dogs, cats, and horses","authors":"Alex Mau,&nbsp;S. M. Keller,&nbsp;Amir Kol","doi":"10.1111/vcp.13404","DOIUrl":"10.1111/vcp.13404","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Basophils are the rarest blood leukocyte in most healthy domestic mammals and the clinical significance of basophilia is poorly understood.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>To empirically determine magnitude thresholds for basophilia, identify its hematologic correlates, and identify associations between breeds, specific diseases, disease categories, organ systems, and basophilia in dogs, cats, and horses.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>CBCs and clinical information from dogs, cats, and horses were collected from the University of California-Davis School of Veterinary Medicine between 2000 and 2020. Relationships between basophil concentration and other CBC parameters were evaluated by computing Pearson's correlation (<i>r</i>). Magnitude thresholds for basophilia (ie, mild, moderate, severe) were determined by evaluating the distribution of basophil counts for each species. For severe cases of basophilia, the clinical diagnoses were categorized by the organ system affected and the underlying pathomechanism. Basophilia groups were compared to a time-matched, randomly selected control group, and chi-square analyses were performed to evaluate associations with disease.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 143 841 (dog), 32 576 (cat), and 44 887 (horse) CBCs were collected. For all three species, basophilia was over-represented in some breeds. Basophilia was associated with respiratory disease in both dogs and cats. In dogs, lymphoma and mast cell neoplasia were associated with basophilia. In horses, an increased incidence of basophilia was not associated with any disease category or organ system.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>This is the largest study evaluating the hematologic correlations and disease associations with basophilia in dogs, cats, and horses. While basophilia was reported alongside many diseases, certain associations may aid clinicians in narrowing down underlying causes.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 1","pages":"24-34"},"PeriodicalIF":1.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.13404","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142772761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of serum protein electrophoresis and immunofixation in dogs seropositive for various vector-borne pathogens","authors":"Oriol Jornet-Rius,&nbsp;Nida Chornarm,&nbsp;Niki Skeldon,&nbsp;Ashley McGrew,&nbsp;Michael Lappin,&nbsp;Laia Solano-Gallego,&nbsp;A Russell Moore","doi":"10.1111/vcp.13399","DOIUrl":"10.1111/vcp.13399","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Canine vector-borne diseases (VBDs) induce non-specific dysproteinemias, detectable by serum protein electrophoresis (SPE). VBDs have been reported to induce a monoclonal gammopathy pattern. Monoclonal gammopathies are commonly the result of paraprotein (M-protein) produced by an immunoglobulin-secreting neoplasm.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>The aims of this study were to characterize and compare SPE and immunofixation (IF) changes, evaluate the performance of previously identified SPE and IF interpretative criteria, and identify M-proteins in a cohort of dogs seropositive for a VBD and with an unknown history for an immunoglobulin-secreting neoplasm.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A total of 143 serum samples from dogs that tested seropositive for different vector-borne pathogens were assessed by SPE. Cases with abnormal globulin fractions were further characterized by IF. Protein fraction and IF labeling results were evaluated using Kruskal–Wallis with Dunn's multiple comparisons and principal component analysis (PCA).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>IF was performed in 112 VBD-seropositive samples with dysproteinemia evaluated by SPE. Most (84/112, 75%) had a polyclonal expansion. Only two dogs had findings suggestive of an M-protein when considering both SPE and immunofixation. PCA clustered E.canis/A.phagocytophilum and B.gibsoni/CM.haematoparvum groups with relatively more γ-globulins than albumin and α-globulins, and the B.gibsoni/CM.haematoparvum group with more prominent IgA and IgM labeling than IgG labeling. Additionally, D.immitis clustered with more prominent β-globulins than γ-globulins and more IgG4 than IgG-FC.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The previously derived interpretative criteria suggested an M-protein in very few VBD-seropositive dogs. PCA identified SPE and immunofixation pattern differences between dogs seropositive for different infectious agents.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"53 4","pages":"420-430"},"PeriodicalIF":1.2,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11735645/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142629334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What is your diagnosis: Acute hemolysis in a Limousin bull","authors":"Alexandre Bertin,&nbsp;Thomas Bonnet,&nbsp;Matthias Lambert,&nbsp;Emi Ludemann,&nbsp;Fabien Corbière,&nbsp;Corine Boucraut,&nbsp;Marie-Noëlle Lucas,&nbsp;Catherine Trumel","doi":"10.1111/vcp.13397","DOIUrl":"10.1111/vcp.13397","url":null,"abstract":"&lt;p&gt;A 4-year-old Limousin bull was presented for necropsy at the Veterinary School of Toulouse. The bull had been on pasture for 2 months. He was depressed and pyrexic (40.9°C) the day before. He was treated with sulfadimidine (90 mg/kg live weight) and flunixin meglumine (2 mg/kg live weight) but died during the night.&lt;/p&gt;&lt;p&gt;The necropsy was performed within 12 h of death and revealed cachexia, pale mucous membranes, few superficial abomasal ulcers, few petechiae of the epicardium and endocardium, diffuse orange discoloration of the liver, severe splenomegaly (85 × 25 cm) and dark red urine. Centrifugation of the urine confirmed the pigmenturia and was highly suggestive of hemoglobinuria. A fine-needle aspiration (FNA) of the spleen was performed (Figure 1).&lt;/p&gt;&lt;p&gt;Samples of spleen (Figure 2A,B), kidney (Figure 3), and liver were fixed in formalin and routinely processed for histopathology. The splenic red pulp was severely congested with numerous pinpoint basophilic inclusions within erythrocytes consistent with piroplasms, and numerous activated macrophages, some of which contained a mixed brown to green dark pigment, positive to Perl's Prussian stain. The white pulp was moderately hyperplastic. The renal cortex was diffusely congested with erythrocytic parasites, and some urinary tubules were necrotic with an accumulation of a cytoplasmic brown-to-green pigment, probably a mix of hemoglobin and biliary pigments. Lesions were consistent with a generalized parasitemia associated with intravascular and extravascular hepatocytic and splenic hemolysis responsible for centrolobular anoxic hepatocytic necrosis and cholestasis, bilirubinuric and hemoglobinuric nephrosis, and diffuse reactive splenitis.&lt;/p&gt;&lt;p&gt;Real-time PCR with high specificity to the 18S RNA gene (&lt;i&gt;Babesia&lt;/i&gt; spp./&lt;i&gt;Theileria&lt;/i&gt; spp.) was performed on a spleen sample and was strongly positive. Subsequently, a fragment of 959 bp of the 18S RNA gene was sequenced and revealed a high homology (97.2% on 959 bp and 99.7% on an internal fragment of 452 bp) with several &lt;i&gt;Theileria orientalis&lt;/i&gt; isolates.&lt;/p&gt;&lt;p&gt;The macroscopic lesions identified at necropsy, coupled with the presence of suspected hemoglobinuria upon urine centrifugation, strongly suggested intravascular hemolytic anemia. Piroplasmosis is commonly reported in the area where the bull came from and was, therefore, a primary differential. Other major causes of hemoglobinuria include &lt;i&gt;Clostridium perfringens&lt;/i&gt; or &lt;i&gt;Leptospira&lt;/i&gt; spp. infections, but these were not investigated further after examination of the FNA.&lt;/p&gt;&lt;p&gt;Theileriosis is a tick-borne disease that causes losses in the cattle industry. Several species of &lt;i&gt;Theileria&lt;/i&gt; can infect bovines, with &lt;i&gt;T. annulata&lt;/i&gt; and &lt;i&gt;T. parva&lt;/i&gt; being highly pathogenic. Conversely, &lt;i&gt;T. orientalis&lt;/i&gt; complex usually causes mild or asymptomatic disease in endemic regions. However, an increasing number of oriental theileriosis outbreak cases have been reported in bo","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 S1","pages":"S54-S56"},"PeriodicalIF":1.1,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.13397","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142508971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of renal tubular transporter proteins in canine urinary extracellular vesicles using liquid chromatography-tandem mass spectrometry.","authors":"L J McGravey, P R Antrobus, T L Williams","doi":"10.1111/vcp.13393","DOIUrl":"https://doi.org/10.1111/vcp.13393","url":null,"abstract":"<p><p>Urinary extracellular vesicles (UEVs) are membranous particles that carry renal tubular transporter proteins. Here, we evaluate whether selected renal tubular transporter proteins can be detected in UEVs isolated from small volume (1-5 mL) canine urine samples of healthy dogs and canine patients with elevated circulating parathyroid hormone (PTH)/PTH-related peptide (PTHrp) concentrations, hypercortisolism, and primary hypoadrenocorticism using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The total creatinine content of each urine sample was calculated from urine volume and creatinine concentration. UEVs were isolated by size exclusion chromatography prior to quantification by nanoparticle tracking analysis and proteomic analysis by LC-MS/MS. Group comparisons were made using non-parametric statistics. Aquaporin-2 (AQP2) and the renal sodium/phosphate co-transporter (NPT2A) were detected in UEVs isolated from small volume samples of almost all healthy dogs but were not detected in most dogs with elevated circulating PTH/PTH related peptide (PTHrp) concentrations, hypercortisolism and primary hypoadrenocorticism. Total creatinine content of the urine sample was strongly positively correlated with the number of UEVs (r_s = .84, P < .001); thus, total creatinine was used as a surrogate marker of UEV number. In healthy dogs, AQP2 and NPT2A were both detected in samples containing at least 1.7 × 10⁹ UEVs or 24 μmol creatinine, however in non-healthy dogs, AQP2 and NPT2A were not detected in most samples containing up to 6.3 × 10⁹ UEVs or 32 μmol creatinine.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142476006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discrepancies of bovine haptoglobin concentrations between serum and plasma using two different anticoagulants and a colorimetric assay based on peroxidase activity","authors":"R. Schmitt,&nbsp;R. Staufenbiel","doi":"10.1111/vcp.13386","DOIUrl":"10.1111/vcp.13386","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Haptoglobin (Hp) is an emerging diagnostic marker in cattle, and knowledge of suitable sample types and measurement methods is important.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>The aims of this study were to compare the results of a colorimetric assay (CA) and an ELISA for bovine Hp using serum, EDTA plasma, and lithium-heparinized (LH) plasma, respectively, and to assess the diagnostic potential for puerperal metritis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>In experiment 1, Hp was measured in pooled aliquots of serum (<i>n</i> = 10), EDTA plasma (<i>n</i> = 10), and LH plasma (<i>n</i> = 10) of 100 healthy fresh lactating dairy cows from 10 farms using both the CA and the ELISA. In experiment 2, five healthy and five cows with acute puerperal metritis were sampled, and Hp was determined using both assays for all three sample types. In experiment 3, aliquots of serum and LH plasma from cows in different lactation stages were transferred into plain, EDTA-coated, and LH-coated tubes and mixed before colorimetric analyses. Distilled water was also placed into each tube type and treated similarly.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Plasma samples measured with the CA showed on average 2.3 (EDTA) and 2.5 (LH) times higher Hp concentrations compared with serum, whereas no differences were seen with the ELISA results between sample types. Based on a clinical cut-off value, both methods differentiated sick from healthy cows. Haptoglobin measurements with the ELISA were less precise compared with CA measurements due to high dilutions. No influence of the anticoagulants on the CA was observed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Due to measurement discrepancies between serum and plasma, CAs for bovine Hp based on peroxidase activity should be performed with serum, or specific reference ranges for plasma samples should be established. In this study, CA results obtained with LH plasma were more precise than results obtained with EDTA plasma. Both the CA and the ELISA are suitable diagnostic tools for the diagnosis of puerperal metritis, but CA measurements were more precise in this study.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"53 4","pages":"409-419"},"PeriodicalIF":1.2,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11735647/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142476007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What's your diagnosis? Circumferential small intestinal mass in a cat","authors":"Cheryl L. Auch,&nbsp;Nutnapong Udomteerasuwat","doi":"10.1111/vcp.13396","DOIUrl":"10.1111/vcp.13396","url":null,"abstract":"&lt;p&gt;An 8-year-old, castrated male domestic shorthair cat was presented to their veterinarian to investigate potential causes for vomiting and weight loss (0.45 kg) over the past 9-month period. On a CBC (ProCyte, IDEXX Laboratories), eosinophilia (4860/μL; reference interval: 170–1570) was noted, and a heparinized plasma chemistry panel was unremarkable. Abdominal ultrasound identified a 2.5-cm circumferential, irregular duodenal mass, and fine-needle aspiration biopsies were obtained. The prepared smears were submitted for cytologic evaluation (Figure 1A–D). Additional abnormalities identified via ultrasound included a thickened gallbladder wall, decreased corticomedullary definition in both kidneys and mild mesenteric lymphadenomegaly. The remainder of the abdominal ultrasound evaluation was unremarkable.&lt;/p&gt;&lt;p&gt;After evaluation of the cytologic specimen, the veterinarian performed an exploratory laparotomy. An approximately 3-cm x 3-cm, duodenal, ulcerated mass was identified immediately aboral to the pyloric sphincter and approximately 5 mm from the sphincter of Oddi. A Billroth I procedure with a choleduodenostomy, and resection was required to obtain adequate margins and reroute the biliary system. Due to the concern for possible mast cell disease, splenectomy and excision of the regional lymph node were also performed, along with an incisional biopsy of the left lateral liver lobe.&lt;/p&gt;&lt;p&gt;The tissue biopsies of the duodenal mass, spleen, duodenal lymph node and liver were submitted for histologic evaluation. The duodenal mass showed varying densities of plump spindle cells interwoven by collagenous trabeculae extensively affecting the tunica muscularis, submucosa, and mucosa, as appreciated on H&amp;E and Masson's trichrome sections (Figure 2A,B). The spindle cells exhibited basophilic cytoplasm, distinct cell borders, and round vesiculated nuclei with a single prominent nucleolus (Figure 2C). Anisokaryosis was moderate, with 20 mitotic figures in 2.37-mm&lt;sup&gt;2&lt;/sup&gt;. The mucosal surface was ulcerated and accompanied by degenerate neutrophils, hemorrhage, and bacterial colonies (Gram-positive cocci and Gram-negative rods). No infectious organisms were detected within the mass on the H&amp;E, Gram, Giemsa or PAS-stained sections. Variable numbers of mast cells, highlighted by toluidine blue stain, and eosinophils were intermixed with the spindle cell population (Figure 2C,D). These findings corresponded with a diagnosis of feline eosinophilic sclerosing fibroplasia.&lt;/p&gt;&lt;p&gt;The duodenal lymph node biopsy displayed low numbers of eosinophils and histiocytes scattered within the subcapsular and medullary sinuses, consistent with mild, multifocal, eosinophilic lymphadenitis. Histologically, the liver contained multifocal periportal aggregates comprised of a few small lymphocytes, plasma cells, and rare eosinophils, indicating mild, lymphoplasmacytic and eosinophilic periportal hepatitis. The splenic biopsy was histologically normal.&lt;/p&gt;&lt;p&gt;Feline ga","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 S1","pages":"S29-S32"},"PeriodicalIF":1.1,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.13396","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142475939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}