Veterinary clinical pathology最新文献_第10页

Comparison of Haptoglobin Concentrations Between Microfilaremic and Amicrofilaremic Dogs Infected by Dirofilaria immitis. 微丝虫病犬与非微丝虫病犬接触珠蛋白浓度的比较。

IF 1 4区农林科学

Veterinary clinical pathology Pub Date : 2026-03-01 Epub Date: 2026-01-29 DOI: 10.1111/vcp.70075

Marianna L E Chocobar, David P Eckersall, Rossella Panarese, William Weir, Elizabeth M S Schmidt

{"title":"Comparison of Haptoglobin Concentrations Between Microfilaremic and Amicrofilaremic Dogs Infected by Dirofilaria immitis.","authors":"Marianna L E Chocobar, David P Eckersall, Rossella Panarese, William Weir, Elizabeth M S Schmidt","doi":"10.1111/vcp.70075","DOIUrl":"10.1111/vcp.70075","url":null,"abstract":"Background: Dirofilariosis, a zoonotic disease caused by Dirofilaria immitis, is associated with cardiovascular damage and systemic inflammation in dogs.Objectives: This study aimed to present preliminary data on the evaluation of serum haptoglobin (Hp) concentration as a potential biomarker of inflammation in dogs naturally infected with D. immitis, with and without microfilaremia.Methods: Thirty dogs were categorized into three groups: microfilaremic seropositives (G1, n = 10), amicrofilaremic seropositives (G2, n = 10), and negative controls (CG, n = 10). Serum Hp concentrations were measured using a colorimetric assay and analyzed via one-way ANOVA with Tukey's post-test.Results: Median Hp levels were 10.0 mg/dL (G1), 9.1 mg/dL (G2), and 13.7 mg/dL (CG), with no significant differences among groups. Additionally, no significant correlation was found between microfilarial burden and Hp levels (p = 0.651).Conclusions: Despite D. immitis infection, Hp concentration did not provide evidence of an inflammatory response in G1 and G2. While previous studies reported decreased Hp in microfilaremic dogs, our findings did not confirm this trend. The seropositive dogs in this study did not show clinical signs, indicating they had relatively mild infections, which may at least in part explain these results. The small sample size and lack of other acute-phase protein assessments restrict the generalizability of our findings and, thus, this study provides limited information about acute phase response dynamics. Nevertheless, these preliminary results highlight the complexity of Hp behavior in D. immitis infection and emphasize the need for further research.","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":"30-33"},"PeriodicalIF":1.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146087472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

European Veterinary Clinical Pathologists Workplace Wellbeing Survey. 欧洲兽医临床病理学家工作场所健康调查。

IF 1.1 4区农林科学

Veterinary clinical pathology Pub Date : 2026-03-01 Epub Date: 2026-03-09 DOI: 10.1111/vcp.70084

Kathleen Freeman, Zoe Polizoupoulou, Kostas Papasouliotis, Mark Rishniw

引用次数: 0

Malignant Follicular Tumors With Myxoid Mucopolysaccharide-Like Stroma on Cytology and Histology in a Cat. 猫恶性滤泡肿瘤伴黏液样粘多糖样基质的细胞学和组织学研究。

IF 1 4区农林科学

Veterinary clinical pathology Pub Date : 2026-03-01 Epub Date: 2026-02-25 DOI: 10.1111/vcp.70093

Ester Quilez, James K Chambers, Reika Ueda, Shannon L Donahoe

{"title":"Malignant Follicular Tumors With Myxoid Mucopolysaccharide-Like Stroma on Cytology and Histology in a Cat.","authors":"Ester Quilez, James K Chambers, Reika Ueda, Shannon L Donahoe","doi":"10.1111/vcp.70093","DOIUrl":"10.1111/vcp.70093","url":null,"abstract":"Background: Malignant follicular tumors are rare in cats, and cytologic description from fine needle aspirates is scarce. While a keratinaceous background is typically expected in follicular neoplasms, it may be absent in some cases, complicating cytologic interpretation.Case presentation: A 10-year-old male Burmese cat presented with a subcutaneous abdominal mass cytologically diagnosed as a carcinoma. Approximately 2 years later, three additional subcutaneous masses developed at different locations. Cytologic evaluation of two of the most recently developed masses revealed features suggestive of a malignant melanocytic tumor with a distinctive granular background. However, histopathologic examination revealed infiltrative carcinomas with basaloid and squamous differentiation, ghost cells, keratohyalin granules, and prominent desmoplastic myxoid stroma, features supportive of a malignant follicular tumor. Immunohistochemistry demonstrated strong p63 and CK19 immunoreactivity, weak and variable cytokeratin AE1/AE3 expression, and negative CK15 labeling, supporting follicular origin. Periodic acid-Schiff (PAS) and Alcian blue stains highlighted abundant mucopolysaccharide-rich stroma. The findings were most consistent with malignant trichoepithelioma. Despite surgical excision, the cat developed additional masses and progressive clinical decline, consistent with metastatic behavior.Conclusions: This case highlights the cytologic and histologic complexity of diagnosing malignant follicular tumors in cats. The presence of abundant myxoid-like mucin-rich stroma may be associated with aggressive biological behavior. Malignant trichoepithelioma should remain a differential diagnosis for basaloid epithelial neoplasms in cats, even in the absence of cytologic evidence of keratinization. This report adds to the limited literature on feline malignant follicular tumors and emphasizes the need for further investigation into their diagnostic criteria and biological behavior.","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":"90-96"},"PeriodicalIF":1.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147310951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytologic, Histologic, and Ultrastructural Findings of Spontaneous, Cutaneous Fibrosarcoma in a Koi. 锦鲤自发性皮肤纤维肉瘤的细胞学、组织学和超微结构表现。

IF 1 4区农林科学

Veterinary clinical pathology Pub Date : 2026-03-01 Epub Date: 2026-03-02 DOI: 10.1111/vcp.70096

Elise E B LaDouceur, Adrienne E Barrett, Anibal G Armien, Danielle Lore, Nicole I Stacy

{"title":"Cytologic, Histologic, and Ultrastructural Findings of Spontaneous, Cutaneous Fibrosarcoma in a Koi.","authors":"Elise E B LaDouceur, Adrienne E Barrett, Anibal G Armien, Danielle Lore, Nicole I Stacy","doi":"10.1111/vcp.70096","DOIUrl":"10.1111/vcp.70096","url":null,"abstract":"Objective: In teleosts, it is often difficult to differentiate between cutaneous neoplasms originating from peripheral nerves and fibrous tissues, and chromatophoromas, and ultrastructural examination is frequently required in addition to light microscopy for definitive diagnosis.Method: A 10-year-old koi (Cyprinus rubrofuscus) had a 3-week history of a 3-4 cm, cutaneous, ulcerated, multinodular mass on the dorsum.Results: Fine needle aspiration revealed spindle cells with abundant, cytoplasmic, variably granular magenta pigment and moderate cellular and nuclear atypia. Biopsy was performed several weeks later and revealed neoplastic spindle cells forming intersecting streams separated by collagenous stroma. Ultrastructural examination showed neoplastic cells had cytoplasmic extensions that were continuous with collagen fibrils, consistent with fibrosarcoma; neoplastic cells lacked pigment and refracting organelles, ruling out chromatophoroma. The nature of the intracytoplasmic pigmented material observed on cytology is uncertain. Neither ultrastructural nor histologic correlates for this cytologically evident material were identified, and differentials for the material are discussed.Discussion: In domestic animals, the presence or absence of concurrent inflammation can often be used to differentiate spindle cell neoplasia from fibroplasia on cytology; this feature is less useful in teleosts, however, as sarcomas are often heavily inflamed and chronic granulomas can be composed of a nearly uniform population of spindle cells. Additionally, there is morphologic overlap between poorly pigmented chromatophoromas and variants of soft tissue sarcoma (STS). For these reasons, definitive diagnosis of STS, chromatophoromas, and many other neoplasms in teleosts often requires a combination of light microscopy and transmission electron microscopy.","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":"49-54"},"PeriodicalIF":1.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147345370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Three Procedures for Cytochemical Detection of Leukocyte Alkaline Phosphatase Activity in Dogs With Leukemia. 三种方法检测白血病犬白细胞碱性磷酸酶活性的比较。

IF 1 4区农林科学

Veterinary clinical pathology Pub Date : 2026-03-01 Epub Date: 2026-01-16 DOI: 10.1111/vcp.70090

Tracy Stokol, Alaina M Kosko, Dorothee Bienzle, Kristina Meichner, Nora L Springer, Michael Byron, Martha Hoffman

{"title":"Comparison of Three Procedures for Cytochemical Detection of Leukocyte Alkaline Phosphatase Activity in Dogs With Leukemia.","authors":"Tracy Stokol, Alaina M Kosko, Dorothee Bienzle, Kristina Meichner, Nora L Springer, Michael Byron, Martha Hoffman","doi":"10.1111/vcp.70090","DOIUrl":"10.1111/vcp.70090","url":null,"abstract":"Background: Assessment of alkaline phosphatase (ALP) activity in cytologic smears is used as a phenotyping tool for hematopoietic and solid tissue neoplasms in dogs. Different procedures that vary in substrate-dye combinations, fixative, incubation times, and nuclear counterstains are available for detection of ALP activity. It is unknown if these procedures provide comparable results for phenotyping tumors in the same animal.Objective: To compare results obtained with three ALP cytochemical procedures in blasts in blood and tissue aspirate smears from dogs with previously diagnosed leukemia: naphthol-AS-MX phosphate/fast blue (fast blue), 5-bromo-4-chloro-3-indoxyl-phosphate/nitroblue tetrazolium (BCIP/NBT), and naphthol-AS-BI-phosphate/fast red violet LB (fast red).Methods: Smears of blood, bone marrow, and lymph node from 54 dogs prospectively enrolled in a multi-institutional study on acute leukemia were stained and assessed. One observer counted the percentage of positive blasts and quantified staining intensity on a scale of 1-3. Cases were classified as acute myeloid leukemia, lymphoblastic leukemia/lymphoma, acute leukemia of ambiguous lineage, or acute lineage-negative leukemia based on flow cytometric analysis and myeloperoxidase cytochemical staining.Results: The fast blue procedure yielded a significantly higher median percentage of ALP-positive blasts (48%) than the BCIP/NBT (46%) or fast red (42%) procedure despite similar median staining intensities. The proportion of samples that would have been classified as ALP-positive was similar (40/54 fast blue, 39/54 BCIP/NBT, and 37/54 fast red).Conclusions: Our results indicate that the three procedures can be used interchangeably for determining ALP activity in blasts of dogs with leukemia.","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":"81-89"},"PeriodicalIF":1.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145990834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 1.1 4区农林科学

Veterinary clinical pathology Pub Date : 2026-02-26

引用次数: 0

IF 1.1 4区农林科学

Veterinary clinical pathology Pub Date : 2026-02-25

引用次数: 0

IF 1.1 4区农林科学

Veterinary clinical pathology Pub Date : 2026-02-24

引用次数: 0

IF 1.1 4区农林科学

Veterinary clinical pathology Pub Date : 2026-01-29