Veterinary clinical pathology最新文献

{"title":"What is your diagnosis? Mammary mass in a female dog","authors":"Gabrielle Z. Tres, Igor R. Santos, Milena C. Paz, Carolina B. Brunner, Sarah K. L. P. Ribeiro, Marcele B. Bandinelli, Saulo P. Pavarini","doi":"10.1111/vcp.13392","DOIUrl":"10.1111/vcp.13392","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 S1","pages":"S9-S11"},"PeriodicalIF":1.1,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of the Sysmex XN-V hematology analyzer for feline specimens","authors":"Maud Guerlin,&nbsp;Fanny Granat,&nbsp;Margot Grebert,&nbsp;Jean-Pierre Braun,&nbsp;Anne Geffré,&nbsp;Nathalie Bourgès-Abella,&nbsp;Catherine Trumel","doi":"10.1111/vcp.13377","DOIUrl":"10.1111/vcp.13377","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The Sysmex XN-V is derived from the new Sysmex XN series of human hematology analyzers. The main changes from the previously validated XT-2000iV analyzer include an optic-fluorescent analysis for platelets and a nucleated red blood cell (NRBC) count.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>We aimed to validate the Sysmex XN-V for feline blood following the American College for Veterinary Clinical Pathology and International Council for Standardization in Hematology recommendations.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Feline EDTA blood specimens were analyzed on the Sysmex XN-V to evaluate repeatability, linearity, comparison with the XT-2000iV analyzer and manual methods, stability, and to verify the previously established Sysmex XT-2000iV RIs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Repeatability was excellent for most variables. Visually determined linearity was excellent or good for most variables except eosinophils and platelet variables. The correlation between the XN-V and XT-2000iV analyzers was good (≥0.82) for all variables except reticulocyte indices. Correlations between the Sysmex XN-V and manual differential counts were good to excellent for most variables, acceptable for neutrophils, and fair for monocytes and NRBC. The previously established Sysmex XT-2000iV RIs can be used to interpret results from the Sysmex XN-V analyzer for most variables except red cell distribution width and reticulocyte variables. The RI for platelet variables could not be evaluated because of platelet clumps. Changes in the Sysmex XN-V measurements after storage at 4 and 24°C were similar to those described for the Sysmex XT-2000iV analyzer.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The performance of the Sysmex XN-V analyzer was good and compared favorably with the Sysmex XT-2000iV analyzer.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"53 3","pages":"294-308"},"PeriodicalIF":1.2,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.13377","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142264393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What is your diagnosis? Bronchoalveolar lavage fluid from a pig","authors":"Mireya Smith,&nbsp;Cassan Pulaski,&nbsp;Stephen Santangelo,&nbsp;Stephen J. Divers,&nbsp;Samantha N. Schlemmer","doi":"10.1111/vcp.13375","DOIUrl":"10.1111/vcp.13375","url":null,"abstract":"<p>A 4-month-old castrated male mini pig was presented to a veterinary teaching hospital for evaluation of frequent, dry, nonproductive coughing for at least 3 weeks. The animal and littermate share an indoor enclosure with outdoor access, including daily walks and swims in a nearby lake. No other clinical signs were noted.</p><p>Initial physical examination was unremarkable, but when sedated, occasional crackles were auscultated in the caudal lung fields. A CBC revealed a mild to moderate microcytosis [41.8 fL; reference interval (RI): 49.89–75.21<span>¹</span>], suspected due to young age, hyponatremia, and/or iron deficiency; hematocrit (25.6%), red blood cell count (6.11 × 10⁶/μL), and MCHC (34.7 g/dL) were considered unremarkable for the sex and species.<span>¹</span> Biochemistry abnormalities included mild hyponatremia (130 mmol/L, RI 136.73–150.25<span>¹</span>) and hypochloremia (94 mmol/L, RI 96.24–105.94<span>¹</span>), secondary to recent intravenous fluid administration. A head and chest computerized tomography (CT) scan revealed generalized bronchial thickening and right middle ventral lung lobe consolidation (suggestive of inflammation, e.g., infectious bronchopneumonia) and fluid/soft tissue debris in the trachea (possibly respiratory debris/phlegm or mucosal thickening/tracheitis). Bronchoalveolar lavage (BAL) was performed, and fluid was sent for cytologic evaluation (Figure  1A–D), aerobic bacterial culture, and mycoplasma PCR testing. Fecal direct mount and flotation were also performed.</p><p>Direct, sediment, and cytospin (Figure 1A–D) preparations of BAL fluid (BALF) were lowly cellular with minimal blood on a light purple background with mucinous material. Low numbers of large (approximately 40–50-μm long), extracellular, three-dimensional, ovoid, brightly basophilic structures, some of which had a scalloped edge, were noted on the cytospin preparation (Figure 1A,B). A leukocyte differential revealed approximately 86% large mononuclear cells/macrophages, 7% small lymphocytes, 6% eosinophils, and 1% nondegenerate neutrophils. Large mononuclear cells were occasionally vacuolated and rarely contained dark blue-black pigment (presumed hemosiderin); rare multinucleated cells were seen. Mixed bacterial populations (cocci in chains, plump rods/bacilli) were noted in the background in variably sized aggregates/mats and adhered to uniform squamous epithelial cells (Figure 1C,D).</p><p><b>Cytologic interpretation</b>: Possible increased eosinophil proportion with extracellular, ovoid basophilic structures (parasitic ova vs contaminants); evidence of oropharyngeal contamination.</p><p>Given the age, species, and clinical signs of the patient, we speculated that the brightly basophilic structures could represent parasitic ova (i.e., lungworm).</p><p>The fecal flotation (Figure 2A,B) and direct mount revealed moderate numbers of larvated, thick-shelled ova measuring approximately 4","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 S1","pages":"S48-S50"},"PeriodicalIF":1.1,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.13375","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142269325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leukemia cutis as a prominent clinical sign in a dog with acute myeloid leukemia","authors":"Francesco Rogato,&nbsp;Balazs Szladovits,&nbsp;Henny Martineau,&nbsp;Andrew D. Yale,&nbsp;Jordan L. Mitchell,&nbsp;Emma J. Holmes,&nbsp;Rachel H. Gardner,&nbsp;Alexandra Guillén","doi":"10.1111/vcp.13382","DOIUrl":"10.1111/vcp.13382","url":null,"abstract":"<p>An eight-year-old male neutered crossbreed dog presented with erosive and ulcerative cutaneous lesions in the inguinal regions, the medial aspect of both thighs, and the stifles. Hematologic assessment revealed nonregenerative anemia, thrombocytopenia, and high numbers of neoplastic mononuclear cells with a variable degree of maturation. The mononuclear neoplastic cells, with nuclei measuring 10–20 microns in diameter, accounted for 57% of the nucleated blood cells. In addition, the blood contained increased numbers of mature neutrophils and monocytes with atypical morphology. Cytologic examination of the right popliteal lymph node found high numbers of large mononuclear cells with similar morphology to those in the peripheral blood. Flow cytometry of peripheral blood revealed expression by the mononuclear neoplastic cells of the pan-leukocyte marker CD45 and myeloid markers CD14, MAC387, and myeloperoxidase (MPO). These results confirmed a diagnosis of acute myeloid leukemia (AML). Computed tomography found moderate nodular hepatosplenomegaly and multifocal bi-cavitary lymphadenopathy. Histopathologic examination of biopsies from the cutaneous lesions identified infiltration of the dermis by intermediate to large neoplastic round cells. Further treatment was declined, and the owners elected euthanasia. Postmortem examination confirmed AML involvement in the bone marrow, peripheral and intracavitary lymph nodes, heart, liver, kidney, and skin. Neoplastic cells in the bone marrow and skin showed positive immunolabeling for ionized calcium-binding adaptor protein 1 and MPO. To the best of our knowledge, this is the first report of ulcerative cutaneous lesions observed among the presenting clinical signs in a dog with AML and secondary leukemia cutis.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"53 4","pages":"448-457"},"PeriodicalIF":1.2,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.13382","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142264397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of regression modeling on the assessment and harmonization of a point-of-care analyzer and commercial laboratory analyzer for feline plasma biochemistry testing","authors":"Randolph M. Baral,&nbsp;Bente Flatland,&nbsp;Susan M. Jaensch,&nbsp;Douglas A. Hayward,&nbsp;Kathleen P. Freeman","doi":"10.1111/vcp.13376","DOIUrl":"10.1111/vcp.13376","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Regression describes the relationship of results from two analyzers, and the generated equation can be used to harmonize results. Point-of-care (POC) analyzers cannot be calibrated by the end user, so regression offers an opportunity for calculated harmonization. Harmonization (uniformity) of laboratory results facilitates the use of common reference intervals and medical decision thresholds.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Our aims were to characterize the relationship of results for multiple biochemistry analytes on a POC and a commercial laboratory analyzer (CL) with three regression techniques and to use regression equations to harmonize the POC results with those of the CL. Harmonized results were assessed by recognized quality goals. We used harmonized results to assess the regression techniques.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>After analyzer imprecision assessments, paired clinical samples were assessed with one dataset to calculate regression parameters that were applied to a second dataset. Three regression techniques were performed, and each was used to harmonize the POC results with those from the CL. POC results were assessed for bias and the number of results reaching quality goals before and after harmonization.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>All regression techniques could be used to harmonize most analytes so that 95% of results were within ASVCP TEa guidelines. Harmonization could be further improved with alternate regression techniques or exclusions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Regression offers a means to harmonize POC and CL analyzers. Further work is needed to assess how few samples can reliably be used and to assess likely species differences. No regression technique reliably describes the relationship between methods when correlation is poor.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"53 3","pages":"325-342"},"PeriodicalIF":1.2,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.13376","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142264399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What is your diagnosis? Coelomic mass in an adult male bearded dragon (Pogona vitticeps)","authors":"James Gaffney,&nbsp;Louisa Asseo,&nbsp;Michael M. Garner,&nbsp;Elise E. B. LaDouceur","doi":"10.1111/vcp.13374","DOIUrl":"10.1111/vcp.13374","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 S1","pages":"S57-S59"},"PeriodicalIF":1.1,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141767493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elevated serum gamma-glutamyltransferase activity and immunohistochemistry in two dogs with renal carcinoma","authors":"Devorah M. Stowe,&nbsp;Elizabeth P. Held,&nbsp;Emily A. Cross,&nbsp;Danielle Meritet,&nbsp;Paul R. Hess,&nbsp;Kelli Ferris,&nbsp;Hiroyuki Mochizuki","doi":"10.1111/vcp.13373","DOIUrl":"10.1111/vcp.13373","url":null,"abstract":"<p>During a 3-year time period, a 15-year-old male castrated Terrier mix (dog 1) and a 6-year-old female spayed Labrador Retriever (dog 2) presented to the North Carolina State Veterinary Hospital with similar blood work abnormalities and no significant physical examination findings. A CBC, chemistry panel, and urinalysis performed on both dogs were relatively unremarkable, other than a marked increase in serum gamma-glutamyltransferase (GGT) activity. Through imaging, both patients were diagnosed with a renal mass, and histopathology of both masses revealed a carcinoma. Immunohistochemical staining of the renal mass in both dog 1 and dog 2 were intensely positive for GGT. Dog 1 had the affected kidney removed, which normalized the GGT value. Dog 2 was euthanized, and metastasis to the lung was noted upon postmortem examination. There have been limited case studies documenting an elevation in serum GGT in dogs diagnosed with renal carcinoma. While renal carcinoma is uncommon in dogs, it is an important differential to keep in mind when there is a marked increase in serum GGT without accompanying increases in other measured liver enzymes. In addition, serum GGT can serve as a helpful biomarker for disease resolution and recurrence, as surgical removal of the renal mass (dog 1) led to the resolution of the elevated serum GGT. To our knowledge, this is the first report demonstrating IHC staining for GGT in a canine renal carcinoma.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"53 3","pages":"321-324"},"PeriodicalIF":1.2,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.13373","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141767492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of serum paraoxonase-1 to assess inflammation in horses with colitis","authors":"Malou F. Winther,&nbsp;Josefine Johnsson,&nbsp;Pernille K. Madsen,&nbsp;Tina H. Pihl,&nbsp;Saverio Paltrinieri,&nbsp;José J. Cerón,&nbsp;Donatella Scavone,&nbsp;Luis Pardo-Marin,&nbsp;Stine Jacobsen","doi":"10.1111/vcp.13372","DOIUrl":"10.1111/vcp.13372","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Paraoxonase-1 (PON-1) has been suggested as a marker of inflammation and oxidative stress in horses and could potentially be used for prognostication in horses with colitis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Assessment of PON-1 in horses with colitis and comparison of two methods.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Serum PON-1 was measured by two methods (paraoxon and p-nitrophenyl acetate) in 161 horses with colitis and 57 controls. Follow-up samples obtained during hospitalization were available from 106 horses with colitis. The two methods were compared.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Serum PON-1 was significantly lower in horses with colitis than in healthy horses (<i>P</i> &lt; .0001 for both methods) as well as in nonsurvivors compared with survivors (<i>P</i> = .0141 [paraoxon-based method] and <i>P</i> &lt; .0001 [p-nitrophenyl acetate-based method]), but with marked overlap between groups. PON-1 activity did not change parallel to a change in inflammatory status in response to treatment when assessed at admission and in up to seven follow-up samples. Admission PON-1 activity could not reliably classify horses as survivors or nonsurvivors, with sensitivity and specificity ranging between 53.1% and 72.9%. Results from the two methods were comparable.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Both methods reliably measured serum PON-1 activity. Significant differences in PON-1 activity were found between healthy horses and horses with colitis and between survivors and nonsurvivors. However, PON-1 activity varied considerably within groups. Both the proposed reference intervals as well as alternative cutoff values resulted in suboptimal diagnostic and prognostic performance, and the use of serum PON-1 in horses with colitis thus seems to add little to existing diagnostic and prognostic markers.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"53 3","pages":"358-368"},"PeriodicalIF":1.2,"publicationDate":"2024-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.13372","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What is your diagnosis? Abnormal cluster on the WDF and WNR scattergrams from Sysmex XN-V in a dog","authors":"Theo Chenal,&nbsp;Fanny Granat,&nbsp;Catherine Trumel,&nbsp;Nathalie Bourgès-Abella","doi":"10.1111/vcp.13362","DOIUrl":"10.1111/vcp.13362","url":null,"abstract":"&lt;p&gt;A 4-month-old intact male, Australian shepherd dog was presented to the emergency unit of the veterinary school hospital of Toulouse (France) for the medical care of a canine Parvovirus infection diagnosed from a positive snap test (SNAP Parvo, IDEXX Laboratories, Westbrook, USA). Clinical examination revealed pale mucous membranes and palpable fluid in the abdomen.&lt;/p&gt;&lt;p&gt;A CBC performed with the ProCyte Dx (IDEXX, Westbrook, USA) (Table S1) revealed a marked normocytic normochromic poorly regenerative anemia, mild leukocytosis, neutrophilia, monocytosis, and marked thrombocytopenia with a flag. The thrombocytopenia was suspected to be genuine despite the observation of a few platelet-fibrin clots on the blood smear. An abdominal ultrasound confirmed the presence of a peritoneal effusion. The dog was transfused with compatible blood.&lt;/p&gt;&lt;p&gt;The following day, analysis of the blood and effusion was performed with the Sysmex XN-V (Sysmex, Kobe, Japan) (Table S1), and smears were reviewed. The CBC confirmed the anemia and revealed mild neutrophilia and lymphopenia with an abnormal WDF scattergram (Figure 1). A marked thrombocytopenia with a flag was reported and confirmed by the absence of platelet clumps on blood smear and a manual estimate of platelet count (12.10&lt;sup&gt;9&lt;/sup&gt;/L). The effusion was macroscopically bloody, results were very similar to the CBC (Table S1), and the cytologic appearance confirmed the suspicion of hemoperitoneum, possibly secondary to the thrombocytopenia even though no bruising or petechia were observed; the hemostasis panel (thrombin time, activated partial thromboplastin time and fibrinogen concentration) was within reference interval.&lt;/p&gt;&lt;p&gt;During hospitalization, two additional CBCs performed with the Sysmex XN-V revealed no abnormal cluster.&lt;/p&gt;&lt;p&gt;To confirm our hypothesis, we added 1 μL of ultrasound gel (Supragel, LCH medical product, Paris, France) in 1 mL of fresh canine EDTA-blood specimen using leftover blood from a blood donation.&lt;/p&gt;&lt;p&gt;A CBC and smear examination of both the native specimen and the specimen mixed with the gel were performed. CBC of the native blood specimen revealed mild erythrocytosis, and examination of the blood smear only revealed a few small platelet aggregates. A curvilinear cluster very similar to the one described in our case was observed on both WDF and WNR scattergrams (Figure 4) with the specimen mixed with gel, resulting in a slight overestimation of WBC and eosinophil counts. Other scattergrams were normal. On the smear, granular material similar to the one observed in our case was also visualized.&lt;/p&gt;&lt;p&gt;This case reports an unusual CBC finding characterized by an additional curvilinear cluster on WDF and WNR scattergrams obtained with the Sysmex XN-V analyzer secondary to blood contamination with ultrasound gel. To our knowledge, there are only a few reports of unusual Sysmex XN-V WDF and WNR scattergrams in human and veterinary medicine.&lt;/p&gt;&lt;p&gt;A curvilinear pattern on the WD","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 S1","pages":"S5-S8"},"PeriodicalIF":1.1,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.13362","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141580956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"It's not always histiocytic sarcoma: Immunocytochemistry to identify two unusual tumors in a Bernese Mountain dog","authors":"Claire Quinn,&nbsp;Kayla Harding,&nbsp;Lisa J. Schlein,&nbsp;Jérémie Korchia,&nbsp;Laura L. Coffee,&nbsp;Amy L. MacNeill,&nbsp;Katherine Tucker-Mohl,&nbsp;Dawn L. Duval,&nbsp;Laura E. Brandt","doi":"10.1111/vcp.13360","DOIUrl":"10.1111/vcp.13360","url":null,"abstract":"<p>A 7-year-old female spayed Bernese Mountain dog was presented for evaluation of hematuria. Incidentally, a right stifle sarcoma was diagnosed via cytology, which raised concern for histiocytic sarcoma (given the patient's signalment) versus another joint-associated sarcoma. Histopathology and immunohistochemistry revealed a CD18-negative, non-histiocytic origin cell population. Findings were consistent with a joint-associated grade II soft tissue sarcoma (STS). The patient's hematuria was progressive over 5 months, and urinary bladder transitional cell carcinoma (TCC) was diagnosed via cystoscopy and histopathology. An enlarged right medial iliac lymph node was identified on routine restaging via abdominal ultrasound 3 months later. Cytology of the lymph node revealed a markedly pleomorphic cell population, again raising concern for histiocytic sarcoma (HS). Other differentials included an anaplastic metastatic population from the joint-associated STS or the TCC. Immunocytochemistry revealed a cytokeratin-positive, CD18-, CD204-, and vimentin-negative cell population, consistent with a carcinoma. DNA was extracted from cytology slides to sequence cells for BRAF mutation status. Sequencing revealed a homozygous V596E (transcript ENSCAFT00845055173.1) BRAF mutation, consistent with the known biology of TCC. In neither case was HS truly present in this patient, but immunocytochemistry provided information that helped to optimize the patient's chemotherapy recommendations.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"53 3","pages":"309-314"},"PeriodicalIF":1.2,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.13360","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141459582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}