Veterinary clinical pathology最新文献

{"title":"Comparative Diagnostic Accuracy of Pleural Effusion Classification Methods in Cats: An Analysis of Naturally Occurring Cases.","authors":"A Mazzei, F Busato, G Marceglia, A Zoia","doi":"10.1111/vcp.70059","DOIUrl":"https://doi.org/10.1111/vcp.70059","url":null,"abstract":"<p><strong>Background: </strong>Pleural effusion (PE) in cats can result from transudative or exudative processes. Transudates are caused by decreased colloid osmotic pressure (↓COP) or elevated hydrostatic pressure (↑HP) gradient, while exudates arise from increased pleural capillary permeability. Diagnostic classification approaches traditionally rely on pleural effusion total protein (TP_PE) and total nucleated cell counts (TNCC_PE). In contrast, Light's criteria employing pleural effusion lactate dehydrogenase (LDH_PE), LDH_PE divided by serum LDH (LDH_ratio), and pleural effusion to serum protein ratio (TP_ratio) are more accurate than classification based on TP_PE/TNCC_PE in humans and show promise in cats.</p><p><strong>Objectives: </strong>To assess the diagnostic accuracy of TNCC_PE, LDH_PE, LDH_ratio, TP_PE, and TP_ratio in classifying feline PEs in ↑HP-transudates, ↓COP-transudates, or exudates and to compare simplified Light's criteria (which relies solely on LDH_PE) with Light's criteria and existing veterinary classification schemes based on TP_PE and TNCC_PE (named Vet-A and Vet-B).</p><p><strong>Methods: </strong>Cross-sectional study including 83 client-owned cats with PE.</p><p><strong>Results: </strong>There were 55 exudates, 28 ↑HP-transudates, and 0 ↓COP-transudates. All the variables analyzed were significantly different between exudates and transudates. Simplified Light's criteria correctly classified 50/55 exudates and 26/28 ↑HP-transudates (sensitivity = 91%, specificity = 93%, accuracy = 92%). Light's criteria correctly identified 55/55 exudates but misclassified 15/28 ↑HP-transudates as exudates (accuracy = 82%). Traditional veterinary schemes showed lower accuracies: Vet-A (57%) and Vet-B (74%). Simplified Light's criteria outperformed Vet-A (p < 0.001) and Vet-B (p = 0.007) and trended higher than Light's criteria (p = 0.096).</p><p><strong>Conclusions: </strong>Simplified Light's criteria demonstrate excellent diagnostic accuracy, outperforming traditional veterinary classification schemes.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145245521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Your Diagnosis? Dermal Mass From the Left Forelimb of a Dog.","authors":"Heather D McDavid, Miranda D Vieson, Robert Armentano, Nicholas T Noto","doi":"10.1111/vcp.70057","DOIUrl":"https://doi.org/10.1111/vcp.70057","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145239771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reference Intervals of Selected Serum and Plasma Biochemical Analytes in Clinically Healthy Multiparous Holstein Cows During the Periparturient Period.","authors":"Nektarios Siachos, Georgios Oikonomou, Nikolaos Panousis, Ioannis Sampsonidis, Stavros Kalogiannis, Georgios Arsenos, Georgios E Valergakis","doi":"10.1111/vcp.70044","DOIUrl":"https://doi.org/10.1111/vcp.70044","url":null,"abstract":"<p><strong>Background: </strong>Monitoring the metabolic changes during the periparturient period is crucial.</p><p><strong>Objective: </strong>We aimed to establish reference intervals (RIs) of several serum and plasma biochemical variables in multiparous periparturient Holstein dairy cows.</p><p><strong>Methods: </strong>A total of 238 multiparous dry cows from six commercial dairy farms were enrolled. Blood samples were collected at predetermined time points (21 and 7 days before the expected calving date, 7, 21, and 28 days after calving, ±2 days on all occasions). Samples from 113 cows that met the inclusion criteria were analyzed for plasma and serum biochemical analytes.</p><p><strong>Results: </strong>The lower and upper reference limits were 40.9-49.3 and 102.8-128.5 U/L, respectively, for aspartate aminotransferase; 9.1-14.0 and 33.8-47.8 U/L for γ-glutamyl transferase; 0.07-0.13 and 0.20-0.43 mmol/L for triglycerides; 1.11-1.25 and 3.04-3.52 mmol/L for cholesterol; 0.0 and 3.42-7.78 μmol/L for total bilirubin; 141.0-285.0 and 778.6-1279.3 μmol/L for β-hydroxybutyrate, 0.07-0.22 and 0.42-1.24 mmol/L for non-esterified fatty acids; 50.8-59.7 and 77.4-103.5 g/L for total protein; 23.6-29.2 and 39.4-48.5 g/L for albumin; 19.4-28.3 and 42.0-58.3 g/L for globulin; 0.59-0.70 and 1.30-1.61 for albumin/globulin ratio; 1.3-3.2 and 7.6-9.7 mmol/L for blood urea nitrogen; 42.4-62.8 and 83.1-124.7 μmol/L for creatinine; 1.8-3.2 and 9.8-25.1 μmol/L for 3-methylhistidine; and 1.9-2.7 and 10.5-14.8 μmol/L for 1-methylhistidine.</p><p><strong>Conclusions: </strong>The established RIs provide valuable benchmarks with important clinical and research applications in dairy cattle medicine.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145192887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disintegrin-Like and Metalloprotease With Thrombospondin Type 1 Repeat Motifs 13 (ADAMTS13) Activity in 40 Healthy Dogs.","authors":"Ashley R Wilkinson, Priscila Beatriz da Silva Serpa, S Michelle Todd","doi":"10.1111/vcp.70052","DOIUrl":"https://doi.org/10.1111/vcp.70052","url":null,"abstract":"<p><strong>Background: </strong>In humans, deficiency of a disintegrin-like and metalloprotease with thrombospondin type 1 motifs, number 13 (ADAMTS13), a von Willebrand factor (vWF) cleaving protease, is an underlying mechanism for thrombosis. The role of ADAMTS13 in canine thromboembolic disease is largely unknown.</p><p><strong>Objectives: </strong>The aims of this study were to evaluate plasma ADAMTS13 activity in healthy adult dogs using a commercially available human ADAMTS13 activity ELISA kit and to establish its reference interval (RI).</p><p><strong>Methods: </strong>Forty healthy adult dogs were prospectively enrolled. Normal health status was confirmed using physical examination findings, history, and the results of hemogram, biochemical profile, screen test for vector-borne diseases, and urinalysis. Plasma ADAMTS13 activity was assessed using the Diapharma RUO Technozym ADAMTS13 Activity ELISA. The assay's precision, accuracy, and detection limits were also evaluated.</p><p><strong>Results: </strong>The canine reference population was comprised of 21 spayed females, 14 castrated males, 3 intact females, and 2 intact males. The median plasma ADAMTS13 activity in healthy dogs was 0.76 IU/mL (range 0.45-1.04). The RI lower limit was 0.45 IU/mL (90% confidence interval 0.45-0.47). The upper limit, although calculated (1.04 IU/mL, 90% CI 1.04-1.04), likely does not reflect the true RI upper limit but a technical limitation of the assay; therefore, rendering an RI of > 0.45 IU/mL. The intra- and inter-assay precision and accuracy range were 9.6%, 11.5%, -8.8% to 3.4%, respectively.</p><p><strong>Conclusions: </strong>The commercially available human ADAMTS13 activity ELISA kit was suitable to analyze canine samples, and the RI for adult dogs was established.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145192961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Your Diagnosis? Marked Discrepancy Between Sysmex XN-9100V Platelet Optical (PLT-O) and Platelet Impedance (PLT-I) Measurements in a Dog.","authors":"Jelena Palić","doi":"10.1111/vcp.70047","DOIUrl":"https://doi.org/10.1111/vcp.70047","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145178796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Your Diagnosis? Submandibular Mass in a 1-Year-Old German Shepherd Dog.","authors":"Thomas Cotrone, Kelsey Fiddes, Ashlee Urbasic, Darian Gordon, Elizabeth Buckles, Elise E B LaDouceur","doi":"10.1111/vcp.70054","DOIUrl":"https://doi.org/10.1111/vcp.70054","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145178850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryptococcus gattii Infection in the Carpal Joint of a Goat.","authors":"Sarah N Bosch, Peres Badial, Heather A Roe, Tomer Shua-Haim, Oscar Alas, Hayley Paradise, Airtor Gallastegui, Diego De Gasperi, Ian K Hawkins, Aline Rodrigues Hoffmann, Christopher J Lanier, Tara P Arndt","doi":"10.1111/vcp.70048","DOIUrl":"https://doi.org/10.1111/vcp.70048","url":null,"abstract":"<p><p>A 5-year-old LaMancha goat buck presented for evaluation of a 1-week history of progressive lameness and carpal swelling in the left thoracic limb. Cytology performed on an aspirate from the left radiocarpal joint identified abundant round to oval, thickly encapsulated, extracellular yeast organisms that occasionally exhibited narrow-based budding, features compatible with Cryptococcus spp. yeast. The patient was euthanized due to poor prognosis, and a necropsy was performed. Histopathology confirmed the presence of numerous fungal yeast associated with the left radiocarpal joint. A cryptococcal serum latex agglutination test was negative. However, panfungal PCR and fungal culture identified the organisms as Cryptococcus gattii. This report describes, for the first time, a case of a C. gattii infection affecting the joint of a goat.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of A Novel Indirect Reference Interval Model for Canine Calcium and Alanine Aminotransferase.","authors":"Simone Manzocchi, Liesl J van Rooyen, Graham Stock, Jennifer R Matlow, Mariana Serra","doi":"10.1111/vcp.70042","DOIUrl":"https://doi.org/10.1111/vcp.70042","url":null,"abstract":"<p><strong>Background: </strong>Population-based reference intervals (RIs) are fundamental for appropriate result interpretation. Current recommendations for their determination specify that a direct, de novo approach is preferable, including at least 120 carefully selected subjects. This is not always feasible due to logistical and financial constraints. Recently, several sophisticated algorithms for the indirect estimation of RIs have been proposed in human medicine and described as comparable with direct methods in many cases.</p><p><strong>Objectives: </strong>Preliminary evaluation of the utility and feasibility of novel indirect methods for determining RIs in the veterinary clinical laboratory.</p><p><strong>Methods: </strong>Calcium and alanine aminotransferase (ALT) were selected as representative measurands expected to show Gaussian and non-Gaussian distributions, respectively. After limited data filtering, canine results extracted from a laboratory database were used to estimate indirect RIs and 90% confidence intervals (CIs) using the RefineR algorithm. Using 90% CIs as well as permissible differences (pD), the indirect RIs were compared to direct RIs derived by the same laboratory, over the same period, from a population of healthy canine blood donors.</p><p><strong>Results: </strong>The direct and indirect approaches yielded comparable reference limits for both measurands. Apart from the upper reference limit for Ca, 90% CIs for the reference limits calculated from the conventional direct and indirect methods overlapped. Direct reference limits were within pD calculated from the indirect reference limits.</p><p><strong>Conclusion: </strong>The indirect method, using the RefineR algorithm, may represent a cost-effective option for RI determination. It may also complement direct approaches and has the potential to be more representative of animal populations.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145131975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Magnification, Image Type, and Number on Convolutional Neural Network Performance in Differentiating Canine Large Cell Lymphoma From Non-Lymphoma via Lymph Node Cytology.","authors":"Christina Pacholec, Hehuang Xie, Julianne Curnin, Amy Lin, Kurt Zimmerman","doi":"10.1111/vcp.70056","DOIUrl":"https://doi.org/10.1111/vcp.70056","url":null,"abstract":"<p><strong>Background: </strong>Lymph node (LN) aspirates are often obtained to distinguish large-cell lymphoma (LCL) from non-lymphoma (NL) in dogs with enlarged lymph nodes.</p><p><strong>Objective: </strong>Images from cytology slides tested the effects of magnification, image type, and number on a convolutional neural network (CNN) differentiating canine LCL from NL.</p><p><strong>Methods: </strong>Three hundred images of LCL and NL were used to train a CNN and interrogate the effects of image magnification, type, and number on the model's performance. Identified cases were imaged at 200×, 500×, and 1000× magnification in color and gray-scale and then used to train and identify optimal magnification and image type. The impact of the image number per cohort (50, 100, 150, 200, 250, 300) on the top model's performance was then assessed.</p><p><strong>Results: </strong>The highest performance with color images was achieved at 1000× magnification, with an accuracy of 95.8%, a Receiving Operating Characteristic (ROC) area of 0.997, and an F-measure of 0.958. Similarly, the best results with gray images, also at 1000× magnification, showed an accuracy of 96.67%, a ROC area of 0.994, and an F-measure of 0.967. Performance improvements were most significant and plateaued as the number of images per class approached 150, with an accuracy of 95%, ROC area of 0.939, and F-measure of 0.95.</p><p><strong>Conclusion: </strong>The analysis across models suggests that color versus greyscale did not significantly impact overall performance to distinguish LCL or NL. Optimal magnification was 1000×. A minimum of 150 images per class is recommended for pilot CNN studies in this 2-class problem.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145114286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Your Diagnosis? Abnormal WDF Scattergram From the Sysmex XN-1000V in a Cat.","authors":"Javier Martínez-Caro, Laura Chaves-Falcón, Marta Lemos, Xavier Roura, Luis Bosch, Josep Pastor","doi":"10.1111/vcp.70030","DOIUrl":"https://doi.org/10.1111/vcp.70030","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145092542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}