Veterinary clinical pathology最新文献

{"title":"The Effect of Analytic Variation on Empirical Biological Variation-Derived Analytical Performance Specifications in the Veterinary Clinical Pathology Laboratory.","authors":"A Russell Moore, Addison Adams","doi":"10.1111/vcp.70053","DOIUrl":"https://doi.org/10.1111/vcp.70053","url":null,"abstract":"<p><strong>Background: </strong>The empirical biologic variation (EBV) method to derive analytical performance specifications (APSs) has been provisionally evaluated for use in the veterinary clinical pathology laboratory. Analytical performance can affect EBV APSs. It has been argued that this effect is minimal, but the effect has not been evaluated for the range of performance found in veterinary laboratories.</p><p><strong>Objective: </strong>Model and compare EBV APSs derived across a range of analytical CV (CV_A) observed in veterinary clinical pathology laboratories.</p><p><strong>Method: </strong>Published data on biochemistry measurand CV_A in veterinary clinical pathology laboratories and dog reference intervals (RIs) were used to model EBV APSs using Monte Carlo simulation. The modeled EBV APSs were compared with analogous APSs from total allowable error (TE_A) and traditional BV models.</p><p><strong>Results: </strong>Generally, the EBV-recommended permissible CV_A (pCV_A) was smaller than the observed CV_A when analytical performance was poor. The EBV APSs did not have a consistent relationship with analogous APSs from TE_A or traditional BV systems when analytical performance was judged acceptable using TE_A or BV APSs. When analytical performance was unacceptable, the resulting EBV APSs increased dramatically (sometimes by > 200%) and lost their initial relationship with TE_A or traditional BV-derived APSs. Using log-normal calculations for measurands with a Gaussian distribution produced wider EBV APSs.</p><p><strong>Conclusion: </strong>The EBV method produces growth-oriented APSs under all modeled conditions and can produce notably more lenient APSs for poorly performing labs than better performing labs. The EBV method can produce acceptable APSs under some conditions and may not be suitable for use in veterinary medicine.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Shapes of Yolk","authors":"Nicole I. Stacy,&nbsp;Michael G. Garner,&nbsp;Amy Alexander,&nbsp;Francisco O. Conrado","doi":"10.1111/vcp.70060","DOIUrl":"10.1111/vcp.70060","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 3","pages":"197-199"},"PeriodicalIF":1.1,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145006627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Textbook of Small Animal Pathophysiology. 1st ed.By Stephan Neumann, Hoboken, NJ; Chichester: Wiley Blackwell (John Wiley & Sons Ltd), 2024, 548 pp. $89.95 (hardback). ISBN: 978-1-11-982461-9","authors":"Cassia Hare","doi":"10.1111/vcp.70051","DOIUrl":"https://doi.org/10.1111/vcp.70051","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 3","pages":"309-310"},"PeriodicalIF":1.1,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145101011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The evolution of erythrocytes and oxygen delivery in vertebrate animals: Introduction.","authors":"John W Harvey","doi":"10.1111/vcp.70001","DOIUrl":"https://doi.org/10.1111/vcp.70001","url":null,"abstract":"<p><p>This manuscript provides a brief introduction and overview of four companion papers that focus on the evolution of oxygen uptake and delivery to tissues. Although major emphasis is placed on the characteristics of erythrocytes and the hemoglobin they contain and how these characteristics vary by taxa in response to environmental influences and metabolic requirements, the accompanying evolution of the cardiovascular and respiratory systems and their interactions with erythrocytes are also discussed. In addition, the similarities and differences in erythropoiesis and the presence of immature erythrocytes in blood across taxa are presented.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum Total Thyroxine Concentrations in Clinically Healthy Pet Rabbits","authors":"Jana Liebscher,&nbsp;Elisabeth Müller,&nbsp;Kerstin Müller","doi":"10.1111/vcp.70041","DOIUrl":"10.1111/vcp.70041","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>In small animals, serum total thyroxine concentration (TT₄) is an important parameter used to assess thyroid function in patients. In rabbits, no information is available on the prevalence of thyroid disease and its diagnosis by laboratory methods. The aim of this study was to establish a device- and method-specific reference interval for TT₄ in clinically healthy rabbits.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives and Methods</h3>\u0000 \u0000 <p>TT₄ concentrations were determined by chemiluminescence assay (CLIA) in 120 clinically healthy pet rabbits (median age 36 months); the study population was divided into age groups (Group 1: 7–24 months, Group 2: 25–59 months, Group 3: &gt; 60 months). The pet rabbits were kept indoors, outdoors, and indoor/outdoor. The reference interval (95 percentile) was calculated using SPSS (IBM, version 28.0) according to the standard of the ASVCP.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A median TT₄ concentration of 0.8 μg/dL (10.28 nmol/L) and a reference interval of 0.6–1.98 μg/dL (7.71–25.44 nmol/L) were determined. No statistically significant differences were found for sex (<i>p</i> = 0.889), age (<i>p</i> = 0.176), and between animals in indoor and outdoor housing (<i>p</i> = 0.967). Highly significant differences were observed between indoor and indoor/outdoor housing (<i>p</i> = 0.002) and significant differences between outdoor and indoor/outdoor housing (<i>p</i> = 0.006).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Concentrations of TT₄ in pet rabbits measured by chemiluminescence appear to be independent of sex and age. Laboratory and device-specific reference intervals for pet rabbits should be used to avoid the influence of different methods, devices, calculation methods, and sample size.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 3","pages":"251-257"},"PeriodicalIF":1.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12444003/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Are Immune-Integrative Biomarkers Predictors of Systemic Inflammation in Show Jumping Horses?","authors":"Carlos Donato Barbosa Alves-Junior,&nbsp;Tiago Cunha Ferreira,&nbsp;Diana Célia Sousa Nunes-Pinheiro","doi":"10.1111/vcp.70034","DOIUrl":"10.1111/vcp.70034","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This study evaluated the dynamics of blood immune-inflammatory biomarkers in response to exercise-induced changes in jumping horses.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>For this purpose, adult Brazilian Sport Horses (<i>n</i> = 9), aged 10.1 years, both sexes, underwent a specific training test consisting of warm-up, flatwork, and jumping exercises, and recovery on a 900 m² sand track over 40 min. Clinical examinations and blood collections were performed at three time points: before (T0), immediately after (T1), and 1 h after (T2) the final exercise. Hematologic and biochemical parameters, such as total protein, globulin, albumin, creatinine, lactate, glucose, creatine kinase (CPK), and lactate dehydrogenase (LDH) were determined, and thus were calculated the following biomarkers: neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), neutrophil-to-albumin ratio (NAR), albumin-to-globulin ratio (AGR), albumin-creatinine ratio (ACR), systemic immune-inflammation index (SII), and systemic inflammatory response index (SIRI). Data were analyzed using Shapiro–Wilk tests, ANOVA, Tukey tests, Kruskal–Wallis, and Dunn tests.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Significant changes were found in heart rate (HR) and respiratory rate (RR), as well as in lactate, CPK, LDH, and glucose levels over time. Regarding the biomarkers, significant changes were observed: T0 to T1 in NAR, PLR, SIRI, and SII; T1 to T2 in NAR, PLR, NLR, AGR, SIRI, and SII; T0 to T2 in SIRI.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>This study demonstrates that equestrian exercises promote changes in integrative immune-inflammatory biomarkers, making them promising for monitoring the performance of jumping horses.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 3","pages":"271-280"},"PeriodicalIF":1.1,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hematology, Clinical Biochemistry, and Blood Cell Morphology Features of Captive Bothrops jararaca From Southeast Brazil","authors":"Amanda O. Alcantara,&nbsp;Bianca M. M. Reis,&nbsp;Juliana G. Fonseca,&nbsp;Maíra A. C. Sousa,&nbsp;Vitor Hugo A. Roxo,&nbsp;Guilherme J. Souza,&nbsp;Fúlvia de Fátima A. Castro,&nbsp;Guilherme N. Souza,&nbsp;Jorlan Fernandes,&nbsp;Elba Regina S. Lemos,&nbsp;Aline M. Souza","doi":"10.1111/vcp.70032","DOIUrl":"10.1111/vcp.70032","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Snakebite envenomation is a worldwide public health issue, particularly relevant to low and middle-income countries. <i>Bothrops</i> spp. antivenom is needed for snakebite treatment, which requires the maintenance of healthy snakes in captivity. Hematology and clinical biochemistry are important tools for monitoring the health status of these animals, as well as detecting hemoparasites (i.e., <i>Hepatozoon</i> spp.).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This study aims to establish hematologic and biochemical reference intervals in a population of captive <i>B. jararaca</i> in a Brazilian serpentarium and assess blood cell morphology.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Blood samples of 32 specimens of captive <i>B. jararaca</i> were collected and analyzed for complete blood count and biochemical variables, such as total proteins, albumin, cholesterol, triglycerides, calcium, phosphorus, urea, creatinine, uric acid, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma-glutamyl transferase, and creatine kinase. Blood smears were also evaluated to investigate blood parasites. Each variable was statistically analyzed according to the ASVCP guidelines and compared between sexes and the occurrence of <i>Hepatozoon</i> spp.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>This study establishes the hematology and clinical biochemistry reference intervals and describes <i>B. jararaca</i> blood cell morphology. We observed no differences between sexes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Our study provides the first reference intervals for hematologic and biochemical variables for <i>B. jararaca</i> on the basis of ASCVP recommendations. The RI described could be essential for the management and treatment of <i>B. jararaca</i> kept under similar captive conditions.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 3","pages":"221-229"},"PeriodicalIF":1.1,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12444004/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Your Diagnosis? Fine-Needle Aspirate From a Lingual Mass in a Cat.","authors":"Emma Stacey, Andrii Trokoz, Kimberly Williams, Dorothee Bienzle","doi":"10.1111/vcp.70040","DOIUrl":"https://doi.org/10.1111/vcp.70040","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Your Diagnosis? Blood Smear and Scatter Plots From a Dog.","authors":"Ioana Sandu, Georgiana Deak, Cosmin Muresan, Peter James O'Brien","doi":"10.1111/vcp.70038","DOIUrl":"https://doi.org/10.1111/vcp.70038","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Three Feline Crossmatch Methods—Tube, Gel Column, and Immunochromatographic Strip","authors":"Victoria K. DiCiccio,&nbsp;Rebecka S. Hess,&nbsp;Nicole M. Weinstein,&nbsp;Samantha Fromm,&nbsp;Ruth Gonzalez,&nbsp;Kimberly Marryott,&nbsp;Mary Beth Callan","doi":"10.1111/vcp.70039","DOIUrl":"10.1111/vcp.70039","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>A pre-transfusion blood crossmatch is recommended to ensure RBC compatibility for previously transfused and transfusion-naïve cats.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>To compare 3 crossmatch methods and anti-feline globulin (AFG) enhancement for determining RBC compatibility.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Prospective study. Major crossmatches were performed using serum from 68 “recipient” cats and RBCs from 44 “donor” cats. Crossmatch methods evaluated include laboratory tube and gel column without and with AFG enhancement and an in-clinic AFG-enhanced immunochromatographic strip.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Tube and gel crossmatches were performed for 211 and strip crossmatches for 133 recipient-donor pairs. RBC incompatibilities were noted in at least 1 crossmatch method for 123 recipient-donor pairs. For determination of the degree of incompatibility, there was a correlation between standard and AFG-enhanced crossmatches for both tube and gel (<i>p</i> &lt; 0.001), standard tube and gel (<i>p</i> = 0.002), and AFG-enhanced tube and gel (<i>p</i> &lt; 0.001). Twelve of 46 and 22 of 113 recipient-donor pairs deemed incompatible with tube and gel, respectively, had RBC agglutination noted only with the addition of AFG. RBC incompatibilities were noted on 15 strips, 4 of which were type A-B mismatched. Odds of detecting RBC incompatibility using strip were 2.9 (<i>p</i> = 0.001) and 6.1 (<i>p</i> &lt; 0.001) times greater with each unit increase in the degree of incompatibility detected by standard and AFG-enhanced tube, respectively.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The clinical relevance of any observed RBC incompatibilities, other than known A-B mismatches, is unknown. While the strip crossmatch is simple to perform and interpret, it might not be sufficiently sensitive to detect weaker RBC incompatibilities.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 3","pages":"230-238"},"PeriodicalIF":1.1,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12444005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}