Veterinary clinical pathology最新文献

{"title":"What Is Your Diagnosis? Superficial Cervical Lymph Node Cytology","authors":"Vinicius N. Hirata, Rúbia S. da Silva, Francisca B. Lang, Augusto de Oliveira, Cinthia M. de Andrade","doi":"10.1111/vcp.70101","DOIUrl":"10.1111/vcp.70101","url":null,"abstract":"<p>An 8-year-old spayed female Maltese dog presented to the Veterinary Hospital of the Federal University of Santa Maria (UFSM) due to a 1-week history of diarrhea with mucus and an episode of vomiting on the day of the consultation. On physical examination, an enlargement was noted in the region of the right mandibular (2.5 × 2 × 1 cm) and superficial cervical (2.7 × 1.5 × 1.5 cm) lymph nodes, with an unknown duration of evolution. No other clinical findings were observed. Hemogram and serum biochemistry tests were requested, and no clinically significant abnormalities were found.</p><p>Fine-needle biopsy (FNB) of both enlarged regions was performed. Slides were stained with aqueous Romanowsky stain, and cytologic analysis was performed. Cytology from the right superficial cervical region is exhibited in Figure 1.</p><p>The patient had a history of mass removal from the right thyroid gland region 18 months prior to presentation. At that time, surgical resection of the mass was performed and sent for histopathologic examination, with a preliminary diagnosis of undifferentiated carcinoma. To determine the cell origin of the poorly differentiated neoplasm, immunohistochemistry (IHC) was performed using 3,3-diaminobenzidine (DAB) and hematoxylin counterstaining. The neoplastic cells immunoreacted with: cytokeratin (AE1–AE3), thyroid transcription factor-1 (TTF-1), chromogranin, synaptophysin, and calcitonin. The proliferation marker Ki-67 was positive in approximately 20% of the neoplastic cells. In conclusion, the immunohistochemical and morphologic profile confirmed the diagnosis of medullary thyroid carcinoma (parafollicular cell/C cell tumor).</p><p>Following the interpretation of the right superficial cervical lymph node and the enlarged mandibular lymph node area, the patient underwent surgical excision of these lesions. Subsequently, the samples were subjected to histopathologic analysis, where the diagnosis of metastatic medullary thyroid carcinoma (Figure 2A) and salivary gland without alterations (data not shown) was concluded. The tissues were sent for IHC, where the cells immunoreacted with TTF-1 (Figure 2B), chromogranin, synaptophysin, AE1–AE3 (Figure 2C), and calcitonin (Figure 2D). The proliferation marker Ki-67 was positive in approximately 20% of the neoplastic cells. The IHC findings for the neoplasm in the lymph node were the same as those for the original tumor previously excised, confirming metastatic medullary thyroid carcinoma.</p><p>Thyroid tumors represent approximately 1%–4% of all diagnosed neoplasms, being the most common endocrine malignancy in dogs, with no sexual predisposition in this species. Boxer, Golden Retriever, and Beagle breeds are overrepresented, and thyroid tumors occur more frequently in older dogs [<span>1-3</span>]. Follicular cell tumors are the most common, with the vast majority being malignant. However, carcinomas of medullary origin are less frequently reported, along with benign neoplasms such as ","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"55 S1","pages":"S5-S8"},"PeriodicalIF":1.0,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.70101","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147692463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Your Diagnosis? Submandibular Mass in a Dog","authors":"Whitney K. Chandler, Barbara E. Powers, Devorah M. Stowe, Nancy B. Collicutt","doi":"10.1111/vcp.70078","DOIUrl":"10.1111/vcp.70078","url":null,"abstract":"<p>A 7-year-old, neutered male, canine Mastiff mix presented to the Oncology Service at a specialty hospital for evaluation of a submandibular mass, difficulty swallowing, and lethargy.</p><p>Physical examination revealed an approximately 4 cm diameter, firm, deep, and adherent mass in the area of the left mandibular salivary gland and a soft, small, left mandibular lymph node. Serum chemistry and complete blood count were unremarkable. Thoracic radiographs and abdominal ultrasound did not reveal evidence of metastasis. Computed tomography confirmed a soft tissue mass originating from the left mandibular salivary gland, measuring 4.9 × 2.8 × 3.3 cm. The mass contained a 3.1 cm tubular extension likely within the sublingual salivary duct, suggesting involvement of the left sublingual salivary gland. In addition, mild-to-moderate left medial retropharyngeal lymphadenomegaly was noted. Fine-needle aspirate biopsy of the left medial retropharyngeal lymph node was performed and submitted for cytologic examination. (Figure 1).</p><p>The sample was highly cellular, consisting of many neoplastic round cells on a pink background with scattered red blood cells. Round cells were predominantly individualized and occasionally arranged in dense aggregates. Cells were plasmacytoid and contained fairly distinct cell borders. Cells displayed a low to moderate nuclear to cytoplasmic ratio with an eccentric, round nucleus with stippled chromatin and a single indistinct nucleolus. Cells contained a moderate to abundant amount of basophilic cytoplasm that occasionally exhibited cytoplasmic blebbing and a faint eosinophilic margin. Cells displayed moderate anisocytosis and anisokaryosis, and occasional binucleation and multinucleation. Occasional small lymphocytes were present. The neoplastic cells displaying plasmacytoid features were suggestive of extramedullary plasma cell tumor metastasis given the presence of a submandibular mass. However, metastasis of a medullary thyroid carcinoma with round or plasma cell appearance could not be entirely excluded. Biopsy with histopathology was recommended for confirmation.</p><p>Left-sided mandibular and sublingual sialoadenectomy and surgical excision of the left, medial retropharyngeal lymph node were pursued. Surgical dissection revealed an encapsulated mass containing the entire left mandibular salivary gland. No margins were reported. Excisional biopsy of the left, medial retropharyngeal lymph node and submandibular mass were submitted for histologic examination. (Figures 2 and 3).</p><p>Histologic evaluation of the retropharyngeal lymph node revealed a focal area with replacement of normal lymph node by sheets of round cells with a moderate amount of cytoplasm. Nuclei were sometimes eccentrically located with a perinuclear clear zone. Nuclei were of intermediate cell size, and mitoses were up to 6 per high-powered field. Cells were contained within the lymph node capsule. The findings were consistent with a malignant pl","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"55 S1","pages":"S16-S18"},"PeriodicalIF":1.0,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.70078","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145990844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Your Diagnosis? Esophageal and Gastric Washes in a Pet Moellendorff's Rat Snake","authors":"Lina C. Bilhalva, Priscila B. S. Serpa, Natalia J. Strandberg, Sriveny Dangoudoubiyam, Craig A. Thompson, Andrea P. Santos","doi":"10.1111/vcp.70073","DOIUrl":"10.1111/vcp.70073","url":null,"abstract":"<p>A 6-year-old female Moellendorff's rat snake (<i>Orthriophis moellendorffi</i>), weighing 0.8 kg, was presented to the Purdue University Veterinary Hospital for evaluation of open-mouth breathing, hypersalivation, and gurgling respiratory sounds. The owner reported that the snake had been quarantined in the serpentarium 1 week prior due to excessive oral mucus, though its appetite remained normal. The owner also disclosed maintaining approximately 50 snakes but provided no further husbandry details. On physical examination, the snake was alert and responsive but exhibited dyspnea. A laryngeal swab was collected for bacterial culture and sensitivity testing; after 5 days, aerobic culture yielded no growth. Ten days later, with no clinical improvement, the snake returned for reevaluation. Bloodwork was unremarkable, but radiographs revealed esophageal dilation with gas and increased soft tissue opacity caudal to the heart, suggestive of esophageal wall thickening or a mass. No radiographic evidence of respiratory disease was observed. Gastric and esophageal washes were obtained for cytologic analysis (Figure 1A,B).</p><p>Both samples exhibited similar features. The samples were of low cellularity, consisting mainly of poorly preserved inflammatory cells and scant erythrocytes in the background. The leukocyte differential consisted of approximately 70% heterophils, 25% macrophages, and 5% lymphocytes. Rare basophils and plasma cells were identified on scanning, along with numerous unremarkable ciliated columnar cells and goblet cells. Notably, sparse thin-walled oval structures (25–35 μm wide × 45–50 μm long) were present. Frequently, a discernible, deeply basophilic granular material consistent with larval forms was seen inside these structures. The cytologic interpretation was mild heterophilic inflammation with larvated ova morphologically compatible with nematode eggs (likely <i>Strongyloides</i> /<i>Rhabdias</i> spp.).</p><p>Smears of the esophageal and gastric washes underwent molecular testing at the University of Florida's Zoological Medicine and Wildlife Disease Laboratory. Genomic DNA was extracted and amplified via PCR using primers NC5 (5′-GTA GGT GAA CCT GCG GAA GGA TCA TT-3′) and NC2 (5′-TTA GTT TCT TTT CCT CCG CT-3′), which target conserved regions flanking the ITS1-5.8S-ITS2 ribosomal complex in nematodes [<span>1</span>]. The amplified PCR products were submitted for Sanger sequencing. Following quality trimming and assembly, a final consensus sequence of 581 bp was submitted to GenBank under accession number PQ303691. A comparison with the database revealed similarity to a previously described <i>Strongyloides</i> sp. (97.7%; GenBank entry: OQ107246.1) in a colony of captive colubrids [<span>2</span>].</p><p>Despite treatment with fenbendazole (for endoparasites) and metronidazole (for potential secondary bacterial infection), the snake's condition deteriorated, necessitating euthanasia. No postmortem examination or fecal analys","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"55 S1","pages":"S68-S69"},"PeriodicalIF":1.0,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.70073","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145432229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Your Diagnosis? Large Mediastinal Mass in a Dog","authors":"Rosane O. Cruz, Talles M. Almeida, Iris D. S. Menezes, Ludmila R. Moroz, Suane N. Boaventura, Tiago Peixoto, Stella F. Valle, Nicole Hlavac","doi":"10.1111/vcp.70064","DOIUrl":"10.1111/vcp.70064","url":null,"abstract":"<p>An 11-year-old mixed-breed female dog was presented with a subcutaneous edema of the cervical region, resulting in coughing and choking. The physical examination revealed muffled lung sounds in the left pulmonary field and tachypnea. There was no lymph node enlargement or abdominal pain. The chest radiograph demonstrated an extensive mass in the right central/cranial hemithorax, partially extending to the left, displacing mediastinal structures. Additionally, there was luminal narrowing of the trachea and hepatomegaly. The computed tomography revealed an intrathoracic mass measuring 8.9 cm in diameter, likely located in the cranial mediastinum, exhibiting foci of mineralization with evidence of vascular invasion in the cranial vena cava, suggesting cranial vena cava syndrome with edema of the head and neck. There was also extensive subcutaneous edema in the cervical region, displacement of the esophagus, brachiocephalic trunk, left subclavian artery, heart, and aortic arch to the left; additionally, there was incarceration, compression, and leftward displacement of the thoracic trachea. An ultrasound-guided fine-needle aspiration (FNA) of the intrathoracic mass was submitted to cytology evaluation (Figure 1).</p><p>Due to the location and extent of the mass, surgical intervention was considered unfeasible, and palliative care was elected. The dog died 1 month after the cytology was collected. A necropsy was conducted, and histopathologic analysis was performed on the mass, esophagus, lung, cranial vena cava, liver, kidneys, lymph node, and heart.</p><p>Histopathologic analysis of the mass revealed an atypical proliferation of moderately pleomorphic chondrocytes arranged in a multinodular pattern. The mass was well delineated, non-encapsulated, and occasionally formed nests, supported by dense fibrovascular stroma. The nuclei were pleomorphic, oval to stellate, small to medium-sized, hyperchromatic, and occasionally eosinophilic, indicating necrosis. Immature cartilage and myxoid/mucinous matrix, as well as osseous metaplasia, were present. Additionally, there were foci of necrosis and hemorrhage, along with isolated fragments composed of well-differentiated chondrocytes (Figure 3). A similar neoplastic cellular proliferation was observed in the esophagus and lung. Consequently, the lesion was diagnosed as extraskeletal myxoid chondrosarcoma, likely originating from the trachea, with multiple metastatic foci.</p><p>Extraskeletal chondrosarcoma is a rare tumor in domestic animals, with few reports of the mesenchymal variant and even fewer of the myxoid variant [<span>1</span>]. The origins of extraskeletal chondrosarcomas remain unknown, though it is hypothesized that this tumor may arise from populations of multipotent mesenchymal cells capable of differentiating into cartilage [<span>2</span>].</p><p>In line with the cases described in dogs [<span>1</span>] and cats [<span>2</span>], as well as with the human [<span>3</span>] literature, the h","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"55 S1","pages":"S27-S29"},"PeriodicalIF":1.0,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.70064","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145293858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Your Diagnosis? Submandibular Mass in a 1-Year-Old German Shepherd Dog","authors":"Thomas Cotrone, Kelsey Fiddes, Ashlee Urbasic, Darian Gordon, Elizabeth Buckles, Elise E. B. LaDouceur","doi":"10.1111/vcp.70054","DOIUrl":"10.1111/vcp.70054","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"55 S1","pages":"S37-S40"},"PeriodicalIF":1.0,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145178850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Your Diagnosis? Leukocytosis With Abnormal WDF and WNR Scattergrams From the Sysmex XN-1000V in a Dog","authors":"Javier Martínez-Caro, Marta Lemos, Josep Pastor","doi":"10.1111/vcp.70092","DOIUrl":"10.1111/vcp.70092","url":null,"abstract":"<p>A 12-year-old neutered female Cocker Spaniel dog was presented for consultation due to the presence of a non-pigmented mass on the right mandible. The dog had a previous history of oral melanoma excision. Surgical removal of the new mass was recommended. During preoperative staging, computed tomography revealed a hepatic mass (8 × 12 cm), mandibular and retropharyngeal lymphadenopathy, a gastric lesion consistent with a polyp, and multiple thoracic intervertebral disc protrusions. Cytology of the lymph nodes was consistent with a reactive process.</p><p>A liver lobectomy was performed, and histopathological analysis confirmed a grade I hepatocellular carcinoma with complete surgical margins and no evidence of lymphatic or vascular invasion. Subsequently, a right mandibulectomy was also performed, and the mass was confirmed to be an oral melanoma on histopathology.</p><p>One month later, the dog was presented due to abdominal distension. On physical examination, the dog was alert but apathetic, with abdominal distension, pale mucous membranes, tachycardia, and normothermia. An abdominal ultrasonography was performed, showing marked peritoneal effusion; a heterogeneous liver with irregular margins in one of the lobes; generalized lymphadenopathy; and a heterogeneous spleen with hypoechoic lesions distorting its margins.</p><p>A complete blood count (CBC) was performed using a ProCyte Dx (IDEXX, Westbrook, USA) (Table S1 and Figure S1) and repeated in a Sysmex XN-1000V (Sysmex Corporation, Norderstedt, Germany) (Table 1 and Figure 1).</p><p>Manual WDF regating was performed in the manual analysis (extended) mode (Table 1 and Figure 3), showing a similar proportion of mast cells as manually calculated. Unlike the manual analysis mode that just allows modifying the predefined gates, the manual analysis (extended) mode allows creating the desired number of gates. Sero-hemorrhagic fluid from the abdominal effusion was analyzed, with a total nucleated cell count of 1.14 × 10<sup>9</sup>/L, a RBC count of 0.02 × 10<sup>12</sup>/L, and total proteins (refractometry) of 5 g/dL, consistent with a protein-rich transudate. Cytological evaluation (Figure S2) showed mast cells (43%), non-degenerated neutrophils (32.5%), small lymphocytes (12%), macrophages (9.5%), and eosinophils (3%). The mast cells showed minimal granulation on aqueous Romanowsky staining and were moderately to well granulated with Wright-Giemsa stain. They showed moderate cellular atypia, including moderate anisocytosis and anisokaryosis, and increased nuclear-to-cytoplasmic ratio. The interpretation was neoplastic effusion due to a mast cell tumor. Ultrasound-guided fine needle aspiration of a hepatic lymph node was performed. Cytological examination revealed a moderately differentiated mast cell tumor, with scant lymphoid tissue and eosinophilic inflammation. The overall interpretation was neoplastic mastocytemia and effusion, possibly due to a visceral mast cell tumor. The owners declin","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"55 S1","pages":"S12-S15"},"PeriodicalIF":1.0,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.70092","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145999059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Your Diagnosis? Multiple Subcutaneous Lumps in a Horse","authors":"Federica Meistro, Maria Virginia Ralletti, Riccardo Rinnovati, Alessandro Spadari, Maria Morini","doi":"10.1111/vcp.70069","DOIUrl":"10.1111/vcp.70069","url":null,"abstract":"<p>A 21-year-old Irish Cob gelding was hospitalized, presenting with multiple subcutaneous nodular masses disseminated across the trunk, neck, head, and ventral abdomen. The two larger masses, which presumably appeared about 3 months prior to clinical admission and had been rapidly growing, were located on the lateral aspects of the neck and measured 12 and 10 cm in diameter, respectively (Figure 1). Throughout the rest of the body (head, trunk, withers, both shoulders, and ventral abdomen), the masses exhibited considerable variability in size, ranging from 0.5 to 6 cm in diameter. On dermatologic external examination, all nodules appeared firm, non-painful, and without pruritus, alopecia, ulceration, or fistulation.</p><p>Fine-needle aspiration (FNA) cytology was performed on the mass located on the left side of the neck (Figure 2), and concurrently, a surgical excisional biopsy of the same subcutaneous nodule was performed under local anesthesia for histopathologic examination, fixed in 10% neutral buffered formalin, and routinely processed.</p><p>Gross examination of the surgical specimen revealed a well-defined, firm, nodular mass with a gray-white cut surface. Histopathology disclosed an infiltrative, unencapsulated, highly cellular nodular mass, composed of solid sheets of polygonal to roundish cells supported by scant fibrovascular stroma. The neoplastic cells exhibited spindle to round morphology, measuring approximately 25–30 μm in diameter, with moderately distinct cell borders, an intermediate nucleus-to-cytoplasm ratio, and a moderate amount of granular or rarely vacuolated eosinophilic cytoplasm. The nuclei were oval to indented, paracentrally located, and characterized by coarsely granular chromatin and one or two prominent nucleoli. Mitoses were observed at a rate of 23 in 10 HPF (2.37 mm<sup>2</sup>), with moderate anisocytosis and anisokaryosis. A significant number of bi- or multinucleated neoplastic cells were present, along with infiltrates of small lymphocytes and plasma cells and multifocal areas of coagulative necrosis (Figure 4a,b).</p><p>The final diagnosis of histiocytic sarcoma was confirmed via immunohistochemic analysis, which showed strong immunopositivity of the tumor cells for IBA-1 and lysozyme, in addition to vimentin (Figure 4c,d). Conversely, the neoplastic cells were immunonegative for pan-cytokeratin, CD3, CD20, and desmin.</p><p>The cytologic and histopathologic features observed in this case prompted consideration of three main differential diagnoses: histiocytic sarcoma (HS), cutaneous histiocytic lymphoma, and giant cell tumor of soft part (GCTSP). These entities may share some cytologic or histopathologic features, but differ in cellular composition, behavior, and immunophenotypic profile. However, each of these neoplasms displays distinctive features in terms of cell morphology, tissue architecture, and immunohistochemical profile that support a more precise classification. Histiocytic sarcoma (HS) is ","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"55 S1","pages":"S64-S67"},"PeriodicalIF":1.0,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.70069","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145990818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Your Diagnosis? Blood Smear and Scatter Plots From a Dog","authors":"Ioana Sandu, Georgiana Deak, Cosmin Muresan, Peter James O'Brien","doi":"10.1111/vcp.70038","DOIUrl":"10.1111/vcp.70038","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"55 S1","pages":"S46-S48"},"PeriodicalIF":1.0,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Your Diagnosis? Right frontal lobe mass in a dog","authors":"Erin E. Farrell, Dae Young Kim, Samantha D. Crist, Gayle C. Johnson, Wendy E. Karnia","doi":"10.1111/vcp.70005","DOIUrl":"10.1111/vcp.70005","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"55 S1","pages":"S56-S59"},"PeriodicalIF":1.0,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Your Diagnosis? Globular to amorphous material in peripheral blood smears","authors":"Em Adam, Craig Thompson, Andrea Pires dos Santos, Joanne Messick","doi":"10.1111/vcp.70008","DOIUrl":"10.1111/vcp.70008","url":null,"abstract":"<p>Three 8-week-old research dogs (two male intact Victorian Bulldogs and one female intact Shih Tzu) had peripheral blood submitted to the Purdue University Veterinary Hospital Clinical Pathology Laboratory (PUVHCPL) for a health screening. These dogs were enrolled in a study aimed at understanding the impact of ground transportation on puppy welfare. All research dogs were considered healthy and had no significant abnormalities observed on physical exam. The dogs were not currently being injected with any fluids or medications. However, as a form of pain management during blood draws, lidocaine cream was applied locally at the blood draw site five minutes before blood collection on each dog. The blood draw site was performed with a 22-, 23-, or 25-gauge needle and included either the jugular vein or cephalic vein. The medical technologist at PUVHCPL observed an unknown material observed at the feathered edge of all blood smears from these dogs, which triggered a review by the clinical pathologist for further evaluation.</p><p>The peripheral blood of one Victorian Bulldog was analyzed using the Advia 2120i Hematology System (Siemens, Washington, DC). Additionally, all three research dogs had peripheral blood smears stained with Modified Wright stain and processed using the Siemens Hema-Tek 3000 system. Since the other two research dogs were only submitted for slide review, their blood was not analyzed by the Advia system. The scatterplots from the Victorian Bulldog only showed abnormalities in the “Baso” channel (Figure 1), where a continuous, linear abnormality is seen going through the mononuclear area of the scatterplot and into where basophils are typically located. This abnormality is presumed to be due to the lidocaine gel since no other abnormalities that could cause this change were noted in the dog's peripheral blood. The smears were evaluated by a clinical pathology resident (EA) and deemed to be of good diagnostic quality without evidence of any age-related changes. Leukocyte numbers and erythrocyte density of two dogs were within normal limits. One dog had minimal macrocytic hypochromic anemia. A few reactive lymphocytes, as well as a moderate amount of poikilocytes, rare polychromatophils, clumped platelets, and rare large platelets, were observed on all of the blood smears. Despite clumping and mild size variability, a manual estimate of platelet numbers was within normal limits. In addition, low to moderate amounts of amorphous to globular, purple to magenta, extracellular material was observed at the feathered edge and occasionally in the monolayer of the peripheral blood smears. Differentials for this material included an artifact of sampling or staining precipitate, a proteinaceous or mucinous material, or material associated with the use of lidocaine cream (Uber Numb 5% Lidocaine Numbing Cream, UberScientific, LLC, Lexington, KT)<span><sup>1</sup></span> as part of pain management during blood draws. To verify that this materi","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"55 S1","pages":"S60-S63"},"PeriodicalIF":1.0,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.70008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}