Comparison of Three Feline Crossmatch Methods—Tube, Gel Column, and Immunochromatographic Strip

IF 1.1 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology Pub Date : 2025-08-22 DOI:10.1111/vcp.70039

Victoria K. DiCiccio, Rebecka S. Hess, Nicole M. Weinstein, Samantha Fromm, Ruth Gonzalez, Kimberly Marryott, Mary Beth Callan

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引用次数: 0

Abstract

Background

A pre-transfusion blood crossmatch is recommended to ensure RBC compatibility for previously transfused and transfusion-naïve cats.

Objectives

To compare 3 crossmatch methods and anti-feline globulin (AFG) enhancement for determining RBC compatibility.

Methods

Prospective study. Major crossmatches were performed using serum from 68 “recipient” cats and RBCs from 44 “donor” cats. Crossmatch methods evaluated include laboratory tube and gel column without and with AFG enhancement and an in-clinic AFG-enhanced immunochromatographic strip.

Results

Tube and gel crossmatches were performed for 211 and strip crossmatches for 133 recipient-donor pairs. RBC incompatibilities were noted in at least 1 crossmatch method for 123 recipient-donor pairs. For determination of the degree of incompatibility, there was a correlation between standard and AFG-enhanced crossmatches for both tube and gel (p < 0.001), standard tube and gel (p = 0.002), and AFG-enhanced tube and gel (p < 0.001). Twelve of 46 and 22 of 113 recipient-donor pairs deemed incompatible with tube and gel, respectively, had RBC agglutination noted only with the addition of AFG. RBC incompatibilities were noted on 15 strips, 4 of which were type A-B mismatched. Odds of detecting RBC incompatibility using strip were 2.9 (p = 0.001) and 6.1 (p < 0.001) times greater with each unit increase in the degree of incompatibility detected by standard and AFG-enhanced tube, respectively.

Conclusions

The clinical relevance of any observed RBC incompatibilities, other than known A-B mismatches, is unknown. While the strip crossmatch is simple to perform and interpret, it might not be sufficiently sensitive to detect weaker RBC incompatibilities.

查看原文本刊更多论文

三种猫交叉配型方法-试管、凝胶柱和免疫层析条的比较。

背景：建议输血前血液交叉配型以确保输血前和transfusion-naïve猫的红细胞相容性。目的：比较3种交叉配型方法和抗猫球蛋白（AFG）增强法测定红细胞相容性。方法：前瞻性研究。主要交叉配型使用68只“受体”猫的血清和44只“供体”猫的红细胞。评估的交叉匹配方法包括实验室试管和凝胶柱，没有和有AFG增强和临床AFG增强免疫层析条。结果：试管和凝胶交叉配型211例，条带交叉配型133例。在123对受者-供者的交叉配型中，至少有一种方法发现了红细胞不相容。为了确定不相容的程度，试管和凝胶的标准交叉配型和afg增强交叉配型之间存在相关性(p结论：除了已知的a - b不匹配外，任何观察到的RBC不相容的临床相关性都是未知的。虽然条带交叉匹配执行和解释简单，但它可能不够敏感，无法检测较弱的红细胞不兼容。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary clinical pathology 农林科学-兽医学

CiteScore

1.70

自引率

16.70%

发文量

133

审稿时长

18-36 weeks

期刊介绍： Veterinary Clinical Pathology is the official journal of the American Society for Veterinary Clinical Pathology (ASVCP) and the European Society of Veterinary Clinical Pathology (ESVCP). The journal''s mission is to provide an international forum for communication and discussion of scientific investigations and new developments that advance the art and science of laboratory diagnosis in animals. Veterinary Clinical Pathology welcomes original experimental research and clinical contributions involving domestic, laboratory, avian, and wildlife species in the areas of hematology, hemostasis, immunopathology, clinical chemistry, cytopathology, surgical pathology, toxicology, endocrinology, laboratory and analytical techniques, instrumentation, quality assurance, and clinical pathology education.