Serum Total Thyroxine Concentrations in Clinically Healthy Pet Rabbits

IF 1.1 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology Pub Date : 2025-09-01 DOI:10.1111/vcp.70041

Jana Liebscher, Elisabeth Müller, Kerstin Müller

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Abstract

Background

In small animals, serum total thyroxine concentration (TT₄) is an important parameter used to assess thyroid function in patients. In rabbits, no information is available on the prevalence of thyroid disease and its diagnosis by laboratory methods. The aim of this study was to establish a device- and method-specific reference interval for TT₄ in clinically healthy rabbits.

Objectives and Methods

TT₄ concentrations were determined by chemiluminescence assay (CLIA) in 120 clinically healthy pet rabbits (median age 36 months); the study population was divided into age groups (Group 1: 7–24 months, Group 2: 25–59 months, Group 3: > 60 months). The pet rabbits were kept indoors, outdoors, and indoor/outdoor. The reference interval (95 percentile) was calculated using SPSS (IBM, version 28.0) according to the standard of the ASVCP.

Results

A median TT₄ concentration of 0.8 μg/dL (10.28 nmol/L) and a reference interval of 0.6–1.98 μg/dL (7.71–25.44 nmol/L) were determined. No statistically significant differences were found for sex (p = 0.889), age (p = 0.176), and between animals in indoor and outdoor housing (p = 0.967). Highly significant differences were observed between indoor and indoor/outdoor housing (p = 0.002) and significant differences between outdoor and indoor/outdoor housing (p = 0.006).

Conclusions

Concentrations of TT₄ in pet rabbits measured by chemiluminescence appear to be independent of sex and age. Laboratory and device-specific reference intervals for pet rabbits should be used to avoid the influence of different methods, devices, calculation methods, and sample size.

Abstract Image

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临床健康宠物兔血清总甲状腺素浓度。

背景：在小动物中，血清总甲状腺素浓度（TT4）是评估患者甲状腺功能的重要参数。在家兔中，没有关于甲状腺疾病患病率及其实验室诊断方法的信息。本研究的目的是建立临床健康家兔TT4的设备和方法特异性参考区间。目的与方法：采用化学发光法（CLIA）测定120只临床健康宠物兔（中位年龄36月龄）体内TT4浓度；研究人群按年龄分组（1组7 ~ 24个月，2组25 ~ 59个月，3组60 ~ 60个月）。宠物兔分别饲养在室内、室外和室内/室外。参考区间（95%百分位）采用SPSS （IBM， 28.0版）软件按ASVCP标准计算。结果：TT4浓度中位数为0.8 μg/dL (10.28 nmol/L)，参考区间为0.6 ~ 1.98 μg/dL （7.71 ~ 25.44 nmol/L）。性别（p = 0.889）、年龄（p = 0.176）、室内外饲养（p = 0.967）差异均无统计学意义。室内和室内/室外住房之间存在极显著差异（p = 0.002），室外和室内/室外住房之间存在显著差异（p = 0.006）。结论：化学发光法测定家兔体内TT4浓度与性别、年龄无关。应使用宠物兔的实验室和设备特定参考区间，以避免不同方法、设备、计算方法和样本量的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary clinical pathology 农林科学-兽医学

CiteScore

1.70

自引率

16.70%

发文量

133

审稿时长

18-36 weeks

期刊介绍： Veterinary Clinical Pathology is the official journal of the American Society for Veterinary Clinical Pathology (ASVCP) and the European Society of Veterinary Clinical Pathology (ESVCP). The journal''s mission is to provide an international forum for communication and discussion of scientific investigations and new developments that advance the art and science of laboratory diagnosis in animals. Veterinary Clinical Pathology welcomes original experimental research and clinical contributions involving domestic, laboratory, avian, and wildlife species in the areas of hematology, hemostasis, immunopathology, clinical chemistry, cytopathology, surgical pathology, toxicology, endocrinology, laboratory and analytical techniques, instrumentation, quality assurance, and clinical pathology education.