Veterinary clinical pathology最新文献

{"title":"What Is Your Diagnosis? Splenic Mass in an African Pygmy Hedgehog.","authors":"Gabriela Hartmann, Milena Carolina Paz, Paola Sônego, Ana Carolina Contri Natal, Lívia Surita, Marcele Bettim Bandinelli, Luciana Sonne","doi":"10.1111/vcp.70021","DOIUrl":"https://doi.org/10.1111/vcp.70021","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unusual Electrophoretic Pattern in a Dog Infected With Angiostrongylus vasorum","authors":"Alice Kéfer,&nbsp;Hélène Machiels,&nbsp;Gaëlle Vincken,&nbsp;Clare Pitchford,&nbsp;Élodie Roels","doi":"10.1111/vcp.70026","DOIUrl":"10.1111/vcp.70026","url":null,"abstract":"<div>\u0000 \u0000 <p>A 7-year-old female intact pug was presented for a six-month history of cough, dyspnea, and exercise intolerance. Treatment with fenbendazole was initiated 3 days before referral based on a positive blood quantitative PCR for <i>Angiostrongylus vasorum (A. vasorum)</i> without improvement of the clinical signs. The dog lived in Belgium with a traveling history to Corsica and Canada. On physical examination, the dog was tachypneic with expiratory dyspnea. Mild non-regenerative anemia, monocytosis, eosinophilia, and basophilia were identified on hematology. Biochemistry revealed hyperproteinemia due to hyperglobulinemia. The protein capillary zone electrophoresis (CZE) identified a restricted polyclonal (or oligoclonal) peak in the beta-globulin region and a tall and narrow peak in the gamma-globulin region, suggestive of either restricted polyclonal or monoclonal gammopathy. Further diagnostic investigations included thoracic radiographs, echocardiography, abdominal ultrasound, urinalysis, snap test 4Dx, <i>Leishmania</i> spp. serology, and <i>Leishmania</i> spp. PCR on bone marrow aspirates. Severe proteinuria was noted with a urine protein to creatinine ratio (UPCR) of 7.54. No infectious agents other than <i>A. vasorum</i> were identified. Treatment with fenbendazole was pursued for 7 days, followed by a spot-on application of imidacloprid/moxidectin combined with tapering the anti-inflammatory dose of steroids, with complete resolution of the respiratory signs. At 1-month recheck, hyperglobulinemia and proteinuria completely resolved, and the thoracic radiographic images improved. To the authors' knowledge, this case is the first to describe such a gammopathy associated with chronic angiostrongylosis.</p>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 3","pages":"258-263"},"PeriodicalIF":1.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144761515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Storage at 4°C vs. 23°C–25°C on Complete Blood Counts in Dunkin Hartley Guinea Pigs","authors":"Yan-Ni Huang,&nbsp;Yu-Han Xie,&nbsp;Jian-An Ling,&nbsp;Rui-Ze Chen,&nbsp;Song-Wen Fu,&nbsp;Jing Xie,&nbsp;Michael Aschner,&nbsp;Yue-Ming Jiang","doi":"10.1111/vcp.70031","DOIUrl":"10.1111/vcp.70031","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Dunkin Hartley guinea pig is a common guinea pig strain used by researchers, but studies about the stability of Dunkin Hartley guinea pig blood samples stored at different temperatures are limited.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>The study aimed to evaluate storage factors of complete blood count (CBC) in Dunkin Hartley guinea pigs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We collected 2 mL blood samples from 20 Dunkin Hartley guinea pigs (weighted 250–300 g, 5–6 weeks, 10 males and 10 females) with 2 EDTA-K₂ vacuum tubes and performed CBC with a Hemavet 950FS analyzer at 0, 4, 8, 24, 48, 72, 96, and 120 h after storage at 4°C and 23°C–25°C, respectively. Analysis of the effect of the testing time, storage temperature, and sex on CBC was carried out by three-way repeated measures analysis of variance (RM-ANOVA).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The results showed that white blood cells (WBCs), heterophil, lymphocyte, and monocyte counts, and red blood cells (RBCs) remained stable over time in 4°C storage. Only WBC, monocyte counts, and RBC remained stable over time with storage at 23°C–25°C. No differences were observed between 4°C and 23°C–25°C for WBCs and platelet count (PLT), but PLT was significantly increased from 96 h to 120 h at 4°C storage and at 72 h and 96 h at 23°C–25°C storage.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>When CBC in Dunkin Hartley guinea pigs has to be delayed, storing samples at 4°C is preferred, and the storage time should not exceed 24 h.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 3","pages":"200-210"},"PeriodicalIF":1.1,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144709021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analytical and Quality Control Validation of a Novel Symmetric Dimethylarginine Assay in Dogs and Cats","authors":"Portia Tshidi Mashego,&nbsp;Emma H. Hooijberg","doi":"10.1111/vcp.70037","DOIUrl":"10.1111/vcp.70037","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>An immunoturbidometric assay for symmetric dimethylarginine (SDMA) measurement on automated chemistry analyzers has recently become available.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To perform analytical validation of the EUROLyser SDMA assay in dogs and cats.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Method validation experiments were performed using stored canine and feline serum. Quality control validation was performed according to Westgard. Performance goals were derived from SDMA biological variation data for both species.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Imprecision ranged from 3.7%–7.8% (dogs) to 6.0%–11.8% (cats) with a dispersion of ±35% for dogs and ±44% for cats. The assay showed linearity (up to 85 μg/dL [dogs], 75 μg/dL [cats]); the preliminary LoQ was 9.5 and 6.9 μg/dL, respectively. Recovery was 19.7% and 6.5%, respectively. Severe hemolysis resulted in a significant bias in both species. The EUROLyser method showed a significant negative proportional and constant bias in dogs and a significant positive proportional and negative constant bias in cats, compared to the comparative method. In dogs, the mean bias (−19.5%) and the bias at clinical decision limits exceeded the desirable bias; in cats, the mean bias (−4.0%) and the bias at clinical decision limits was &lt; 8%. Because of high imprecision, the TE_A that could be controlled for was 35%, with a 1–2.5 s rule using patient pools as quality control material.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The novel SDMA assay showed acceptable analytical performance, but high dispersion has consequences for the interpretation of results at reference limits and serial measurements. Method-specific reference intervals and decision limits should be generated for both species, particularly for dogs, given the significant bias vs. the comparative method.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 3","pages":"239-250"},"PeriodicalIF":1.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12444010/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144660420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What's Your Diagnosis? A Case of Extreme Thrombocytosis in a Dog.","authors":"Stephanie F Anderson, King Long Hannah Lee, Megan Dietz, Sarah Guess, Laura White, Mara S Varvil","doi":"10.1111/vcp.70022","DOIUrl":"https://doi.org/10.1111/vcp.70022","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144660422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Testing of In-Clinic, Point-of-Care Hematology Analyzers With a Commercial Laboratory Reference Analyzer","authors":"S. Daly,&nbsp;K. P. Freeman,&nbsp;P. A. Graham","doi":"10.1111/vcp.70023","DOIUrl":"10.1111/vcp.70023","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Previous studies have shown that veterinary in-clinic hematology analyzers may have analytical errors, but few studies have been found that attempt bias correction and look at the effect of this on the achievement of several quality goals and evaluation of analytical performance specifications.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>To design a comparative testing program for use by in-clinic laboratories with a variety of point-of-care (POC) analyzers and to evaluate the performance of in-clinic hematology analyzers compared to a reference analyzer. To determine whether the removal of bias from the in-clinic result can be used in preparation for the harmonization of the results with those of the reference laboratory. To determine the analytical performance specifications/quality goals that can be achieved with the removal of bias from the in-clinic results. To determine whether clinic-based clinicians and technicians found the information provided valuable for ongoing in-clinic hematology quality management.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Fresh EDTA whole blood canine samples with results that were within reference limits were processed five times by in-clinic staff using their in-clinic analyzer before sending the sample to the reference laboratory for comparison. Results were assessed using quality goals, including total allowable error (TE_a) or expert opinion and sigma metrics. The quality goal index (QGI) was calculated for measurands whose performance was &lt; 4 sigma. The potential impact of any difference in results on clinical interpretation was also reviewed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>In comparison to the reference analyzer, in-clinic hematology analyzers often had analytical error and failed to meet quality goals for TE_a and sigma metrics. This error was often undetected by the in-clinic laboratory. Sigma metric &lt; 4 was a useful performance indicator of poor performance, and only 54% of measurands had sigma metrics &gt; 4. Of these poor-performing instances, 90% were attributed to bias. Performance improved for in-clinic analyzers by adjusting results to correct for bias in preparation for harmonization.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>A comparative testing program was successfully designed and implemented to evaluate performance and harmonize in-clinic hematology analyzers to a reference analyzer using samples that are within reference intervals.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 2","pages":"95-105"},"PeriodicalIF":1.2,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144660421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Sensitivity of the PCR for Antigen Receptor Rearrangement (PARR) Assay for Canine Plasma Cell Tumors","authors":"Emily D. Rout,&nbsp;Kacie Seymour,&nbsp;Robert Burnett,&nbsp;Cora Contreras,&nbsp;Anne C. Avery,&nbsp;A Russell Moore","doi":"10.1111/vcp.70036","DOIUrl":"10.1111/vcp.70036","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The PCR for Antigen Receptor Rearrangement (PARR) assay assesses clonality of a lymphoid population. Studies suggest PARR has decreased sensitivity for detecting clonality in canine plasma cell tumors (PCT) compared to lymphoma.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Assess sensitivity of PARR assays targeting the immunoglobulin heavy chain (IGH), immunoglobulin lambda light chain (IGL), and kappa deleting element (Kde) loci in canine PCT.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Canine cases included 35 PCTs (multiple myeloma and extramedullary, cutaneous or oral PCTs), 5 non-PCT foil cases, and 40 B-cell lymphoma cases. PCT diagnoses were confirmed via CD3, PAX5, and MUM1 immunolabeling, and some additionally had histopathology, serum or urine protein electrophoresis, or immunofixation. Nodal B-cell lymphomas were diagnosed by flow cytometry. Routine PARR (targeting IGH V-D-J and D-J rearrangements) and extended PARR (targeting additional IGH genes and IGL and Kde rearrangements) were performed on cytologic specimens (PCT and foil cases) and flow cytometry aspirates (lymphoma cases). Diagnostic sensitivity was calculated.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Across both PARR assays, every PCT case with sufficient sample was interpreted as clonal and no foil cases were clonal. The routine PARR assay detected a clonal immunoglobulin rearrangement in 26/35 PCT cases (74.3%; 95% CI 56.7%–87.5%). The extended assay detected immunoglobulin gene clonality in 33/35 PCT cases (94.3%; 95% CI 80.8%–99.3%). 40/40 lymphoma cases were clonal in both the routine and extended PARR assays.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The combined PARR assays used in this study, evaluating multiple IG loci, detected clonality in all PCTs tested, but routine PARR (targeting IGH) was less sensitive for detecting PCT clonality compared to B-cell lymphomas.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 3","pages":"281-291"},"PeriodicalIF":1.1,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12444009/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144638115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Method Comparison and Investigation of Interference Variables of a Hand-Held Hemoglobinometer(HemoCue Hb 201+) in Cats","authors":"Yuqing Sun,&nbsp;Xiu Ting Yiew,&nbsp;Janet Beeler-Marfisi,&nbsp;Carolyn L. Kerr,&nbsp;Gabrielle Monteith,&nbsp;Shane Bateman","doi":"10.1111/vcp.70035","DOIUrl":"10.1111/vcp.70035","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Regular blood sampling to monitor RBC mass in anemic cats can exacerbate anemia. Laboratory-based reporting can delay clinical decisions. A hand-held hemoglobinometer, HemoCue Hb 201⁺ (HC-201), requires only one drop of blood (10 μL) and provides results within 1 min.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This preliminary study aimed to evaluate the utility of HC-201 in cats and investigate the impact of potential interferents on its performance.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>One hundred and fifty-four venous blood samples in EDTA from 93 cats were analyzed. Hemoglobin concentration was measured once using an ADVIA 2120 analyzer and compared to the mean of 2–3 replicate measurements from an HC-201. Agreement and systematic bias between HC-201 and ADVIA results, along with precision between HC-201 replicates, were assessed using Lin's concordance correlation coefficient, non-parametric Bland–Altman, Passing-Bablok regression, and intraclass correlation coefficient. The performance of HC-201 in the presence of anemia, leukocytosis, azotemia, lipemia, icterus, hemolysis, and peripheral versus jugular venipunctures was assessed using Wilcoxon rank-sum tests.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Passing-Bablok analysis revealed a significant constant bias (intercept = −2.242, 95% CI: −4.042 to −0.667) but no significant proportional bias (slope = 1.015, 95% CI: 1.000–1.032). HC-201 demonstrated excellent agreement (<i>ρ</i>_c = 0.989) and precision (ICC = 0.997) with a median bias of −0.67 g/L (<i>p</i> = 0.001). The total observed error was 3.02%, within the allowable limits defined by international standards. Neither anemia, leukocytosis, azotemia, lipemia, nor venipuncture site influenced HC-201 measurements. Samples with icterus and hemolysis were insufficient in number for statistical comparison.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>This preliminary study indicates that HC-201 offers reliable point-of-care monitoring for hemoglobin concentration in cats.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 3","pages":"211-220"},"PeriodicalIF":1.1,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12444007/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144638116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HARISS: Histogram Analyzer for Reference Intervals of Small Samples, a Free Web App to Calculate Reference Intervals of Small Samples.","authors":"Kevin Le Boedec","doi":"10.1111/vcp.70033","DOIUrl":"https://doi.org/10.1111/vcp.70033","url":null,"abstract":"<p><strong>Background: </strong>Reference interval (RI) estimate inaccuracy is problematic at small sample sizes. Visual assessment of distribution histograms (VADH) may improve statistical technique selection, but its performance depends on the human operator.</p><p><strong>Objectives: </strong>This study aimed to develop a machine learning model for small-sample VADH.</p><p><strong>Methods: </strong>The training set consisted of 45 000 distribution histograms from samples ranging from 20 to 40 individuals extracted from simulated Gaussian, lognormal, and left-skewed populations. A convolutional neural network (CNN) was trained to predict the original population distribution by VADH. Its accuracy was tested on 900 human-classified histograms from a previous study (ranging from 20 to 60 individuals) and compared to the Shapiro-Wilk test performance in determining the original population distribution. A web application was developed to allow usage of the CNN, 95% RI estimation with 90% confidence intervals (CI) via bootstrapping, and outlier detection.</p><p><strong>Results: </strong>The CNN model properly predicted the original population distribution by VADH in 84.0% (95% CI: 83.7-84.4) and 94.4% (95% CI: 92.7-95.8) of the samples of the training and the test sets, respectively. Comparatively, on the test set, the Shapiro-Wilk test accuracy was 65.0% (95% CI: 61.8-68.1) and 72.3% (95% CI: 69.3-75.2) using a p-value threshold of 0.05 and 0.2, respectively. The web application (named HARISS) was successfully deployed and is accessible at: https://hariss.streamlit.app/.</p><p><strong>Conclusions: </strong>The CNN model demonstrated effective VADH and might enhance RI estimate accuracy through the use of the HARISS web app, though proper reference individual selection and preanalytical/analytical conditions remain paramount.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144627206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Reference Intervals for the TEG 6s, a Point-of-Care Viscoelastic Assay, in Dogs","authors":"Ashley R. Wilkinson,&nbsp;Bobbi J. Conner,&nbsp;Priscila B. S. Serpa","doi":"10.1111/vcp.70027","DOIUrl":"10.1111/vcp.70027","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Thromboelastography (TEG) provides a global assessment of hemostasis and identifies patients with both hemorrhagic and thrombotic tendencies. Traditional TEG instruments, such as the TEG 5000 (Haemonetics), can be labor-intensive and require specialized training to operate. The TEG 6s (Haemonetics) is a commercially available point-of-care TEG that employs a cartridge-based system. Agreement between the TEG 6s and TEG 5000 is poor in dogs, and their viscoelastic measurements are not interchangeable. Reference intervals specific to the TEG 6s are needed for healthy adult dogs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This study aimed to establish reference intervals for the TEG 6s in healthy adult dogs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Citrated whole blood samples collected from 40 healthy adult dogs were analyzed using the TEG 6s and associated global hemostasis and lysis cartridges. Reference intervals were established using a nonparametric statistical method for all variables except for the Functional Fibrinogen level in the global hemostasis cartridge.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Reference intervals were established for 12 TEG 6s measurands in healthy adult dogs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The results of this study provide reference intervals for TEG 6s variables associated with the global hemostasis and lysis cartridges in healthy adult dogs. Further studies are required to assess TEG 6s' utility in dogs with various disease states.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 3","pages":"292-299"},"PeriodicalIF":1.1,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12444008/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144627205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}