Veterinary clinical pathology最新文献

{"title":"Response Letter to the Editor.","authors":"","doi":"10.1111/vcp.70152","DOIUrl":"https://doi.org/10.1111/vcp.70152","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149115489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stability of Complete Blood Count Parameters in Rats and Rabbits: Impact of Blood Sampling Volume and Storage Time on Analytical Reliability.","authors":"Yan-Ni Huang, Yi-Ling Li, Yu-Han Xie, Jian-An Ling, Rui-Ze Chen, Song-Wen Fu, Jing Xie, Michael Aschner, Yue-Ming Jiang","doi":"10.1111/vcp.70140","DOIUrl":"https://doi.org/10.1111/vcp.70140","url":null,"abstract":"<p><strong>Background: </strong>In laboratory practice, complete blood count (CBC) analysis is not always immediate, and underfilled or overfilled blood tubes may yield misleading results.</p><p><strong>Objectives: </strong>To assess CBC stability in rats and rabbits over 24 h and determine the effect of under- or over-filled tubes on alterations in the results, demonstrating which indices remain reliable under these conditions.</p><p><strong>Methods: </strong>Blood was collected into EDTA-K₂ vacuum tubes from rats (tubes: 0.5, 1.0, 1.5, 2.0 mL) and rabbits (tubes: 2.0, 2.5 mL). All tubes were stored at 4°C and analyzed at 0, 4, 8, and 24 h using a CBC analyzer.</p><p><strong>Results: </strong>Statistically significant changes (p < 0.05) were observed: In rats, white blood cell (WBC), neutrophil, lymphocyte, monocyte, red blood cell (RBC), hematocrit (HCT), and hemoglobin (Hb) counts were sensitive to blood sampling volume and decreased with smaller volumes. WBC, neutrophil, RBC, HCT, and platelet (PLT) counts showed no statistically significant change within 24 h. In rabbits, blood sampling volume and storage time independently influenced WBC, neutrophil, lymphocyte, and Hb counts. RBC, HCT, and PLT counts showed no statistically significant change within 8 h.</p><p><strong>Conclusions: </strong>WBC, neutrophil, lymphocyte, RBC, HCT, and Hb are volume-sensitive in rats and rabbits, whereas PLT counts are unaffected by volume. Therefore, blood samples with insufficient or excessive fill may still be used for PLT measurement, but results for the other indices are not recommended.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148913448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Your Diagnosis? Subcutaneous Mass on the Chest of a Dog.","authors":"Emily Zuber, Rachel Neto, Robert Cole, Maria C Naskou","doi":"10.1111/vcp.70153","DOIUrl":"https://doi.org/10.1111/vcp.70153","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148892410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eosinophilic Cerebrospinal Fluid Pleocytosis in A Dog With Hypertrophic Pachymeningitis and Generalized Eosinophilic Disease.","authors":"David Daudén, Simon R Platt, Hayley E Crosby-Durrani, Anne Aworinde, Emanuele Ricci, Emily Davies, Emili Alcoverro","doi":"10.1111/vcp.70142","DOIUrl":"https://doi.org/10.1111/vcp.70142","url":null,"abstract":"<p><p>An 8-year-old Whippet crossbreed was presented with a month's history of collapsing episodes and altered mentation. Clinical improvement was noted upon administration of prednisolone prior to referral. Neurologic examination was compatible with a multifocal intracranial disease. Magnetic resonance imaging revealed thickening of the dura mater around the left parietal and temporal cerebral cortex, left piriform lobe, and cerebellar hemispheres. Cerebrospinal fluid (CSF) analysis revealed marked eosinophilic pleocytosis with increased protein concentration. Infectious diseases were ruled out. Thoracic and abdominal imaging did not reveal any significant abnormalities. The dog was euthanized 4 days after diagnosis. Post-mortem examination revealed diffuse, severe, subacute to chronic eosinophilic pachymeningitis and generalized eosinophilic disease. Eosinophilic CSF pleocytosis has been reported in a number of inflammatory (sterile and infectious) and neoplastic canine encephalopathies. To the best of our knowledge, eosinophilic pleocytosis has not been reported in cases of hypertrophic pachymeningitis in dogs.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148892250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Your Diagnosis? Organisms in Cerebrospinal Fluid of a Dog.","authors":"Adriana Alire, Michael Talavera, Thomas S Campbell, Ann Marie McBride, Michaela Beasley, Matthew Williams","doi":"10.1111/vcp.70151","DOIUrl":"https://doi.org/10.1111/vcp.70151","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148892395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two Cases of Feline Hepatosplenic Cytotoxic T-Cell Lymphoma With Hemophagocytosis.","authors":"Hiroki Yamazaki, Yuki Kimura, Seio Onoyama, Hideo Akiyoshi","doi":"10.1111/vcp.70105","DOIUrl":"https://doi.org/10.1111/vcp.70105","url":null,"abstract":"<p><p>This report describes the clinical courses of two cats with hepatosplenic cytotoxic T-cell lymphoma (TCL) with tumor-cell hemophagocytosis (THP). Both cats presented with pancytopenia and marked splenomegaly with a honeycomb pattern on ultrasonography. Cytology of splenic aspirates revealed large atypical lymphocytes with phagocytosis of erythrocytes and leukocytes. Immunohistochemical analysis revealed positive staining for CD3 and granzyme B. Bone marrow analysis revealed preserved trilineage hematopoiesis without apparent evidence of neoplastic infiltration. In Case 1, the cat was treated with prednisolone, L-asparaginase, and a CHOP-based chemotherapy protocol, resulting in temporary improvement. However, the cat died suddenly on day 88, with necropsy revealing pulmonary fibrosis. In Case 2, early splenectomy followed by CHOP chemotherapy led to sustained clinical improvement and longer-term disease control. Despite relapse on day 65, the cat responded to rescue therapy with L-asparaginase and ACNU and survived until day 278. These cases highlight the clinical and pathological features of 2 cases of feline hepatosplenic cytotoxic TCL with THP and suggest that splenectomy may play a role in improving clinical stability by suppressing hemophagocytic activity. A multimodal approach incorporating surgery and chemotherapy may be considered as a therapeutic option for hepatosplenic cytotoxic TCL with THP.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148888706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Your Diagnosis? Rostral Lesions in a Rio Grande Cichlid (Herichthys cyanoguttatus).","authors":"Laura A Cagle, Bridget Walker, Randall Walker, Shir Gilor, Christopher J Lanier, Amy B Alexander, Robert J Ossiboff, Roy P E Yanong, Nicole I Stacy, Sarah L Poynton","doi":"10.1111/vcp.70138","DOIUrl":"https://doi.org/10.1111/vcp.70138","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148867032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letter to the Editor: Veterinary Medicine Needs Harmonization of Numerical Results.","authors":"K P Freeman, S Daly, R Baral","doi":"10.1111/vcp.70145","DOIUrl":"https://doi.org/10.1111/vcp.70145","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148866974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Your Diagnosis? Blood Smear From a North American Beaver (Castor canadensis).","authors":"Lilani Munasinghe, Shanika Kurukulasuriya, Tracy Yuen, Dennilyn Parker, Betty Lockerbie","doi":"10.1111/vcp.70068","DOIUrl":"10.1111/vcp.70068","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":"S74-S76"},"PeriodicalIF":1.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13666551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145432195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary Reference Interval Estimation for Hemolymph Analysis in Free-Ranging Mangrove Horseshoe Crab (Carcinoscorpius rotundicauda).","authors":"Farsia Naher, Md Nayeem Hossain, Md Ridoan Pasha, Akbar John, Sk Ahmad Al Nahid","doi":"10.1111/vcp.70150","DOIUrl":"https://doi.org/10.1111/vcp.70150","url":null,"abstract":"<p><strong>Background: </strong>The mangrove horseshoe crab (Carcinoscorpius rotundicauda), a species thriving in the muddy brackish waters of Cox's Bazar, holds significant ecological and biomedical value. Hematological and biochemical analyses can serve as a valuable diagnostic tool for assessing health; however, reports remain limited for horseshoe crabs.</p><p><strong>Objective: </strong>This study investigated hemolymph values to determine preliminary reference intervals (RIs) from free-ranging mangrove horseshoe crabs.</p><p><strong>Methods: </strong>Forty adult Carcinoscorpius rotundicauda (20 males, 20 females) were collected from intertidal zones of Cox's Bazar in February 2024. Hemolymph was extracted by cardiac puncture under cold anesthesia for amebocyte counting and biochemical profiling. Plasma metabolites, enzymes, and electrolytes were analyzed using a standard automated laboratory biochemistry analyzer (Humalyzer 3000, Human Diagnostics, Germany), and data were statistically evaluated in accordance with established guidelines.</p><p><strong>Results: </strong>Total amoebocyte counts demonstrated substantial variability (RI: 0.6-38.5 × 10⁶ cells/mL). This study found significantly (p < 0.05) higher levels of cholesterol, triglycerides, chloride (Cl^-), calcium (Ca²⁺), magnesium (Mg²⁺), and aspartate aminotransferase (AST) in female horseshoe crabs, whereas alanine aminotransferase (ALT) activity was significantly higher in males. In contrast, no significant (p > 0.05) differences between sexes were observed in glucose, total protein, albumin, alkaline phosphatase (ALP), uric acid, creatinine, urea, blood urea nitrogen (BUN), sodium (Na⁺), potassium (K⁺), and amebocyte counts. Reference intervals (RIs) were established after excluding outliers identified across multiple measurands in both sexes.</p><p><strong>Conclusion: </strong>This study provides baseline data and underscores the need for further research, particularly across different seasons, to enhance the suitability and applicability of the established RIs.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148866992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}