{"title":"方法手持式猫血红蛋白仪（HemoCue Hb 201+）干扰变量的比较与研究。","authors":"Yuqing Sun, Xiu Ting Yiew, Janet Beeler-Marfisi, Carolyn L. Kerr, Gabrielle Monteith, Shane Bateman","doi":"10.1111/vcp.70035","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Regular blood sampling to monitor RBC mass in anemic cats can exacerbate anemia. Laboratory-based reporting can delay clinical decisions. A hand-held hemoglobinometer, HemoCue Hb 201<sup>+</sup> (HC-201), requires only one drop of blood (10 μL) and provides results within 1 min.</p>\n </section>\n \n <section>\n \n <h3> Objectives</h3>\n \n <p>This preliminary study aimed to evaluate the utility of HC-201 in cats and investigate the impact of potential interferents on its performance.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>One hundred and fifty-four venous blood samples in EDTA from 93 cats were analyzed. Hemoglobin concentration was measured once using an ADVIA 2120 analyzer and compared to the mean of 2–3 replicate measurements from an HC-201. Agreement and systematic bias between HC-201 and ADVIA results, along with precision between HC-201 replicates, were assessed using Lin's concordance correlation coefficient, non-parametric Bland–Altman, Passing-Bablok regression, and intraclass correlation coefficient. The performance of HC-201 in the presence of anemia, leukocytosis, azotemia, lipemia, icterus, hemolysis, and peripheral versus jugular venipunctures was assessed using Wilcoxon rank-sum tests.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Passing-Bablok analysis revealed a significant constant bias (intercept = −2.242, 95% CI: −4.042 to −0.667) but no significant proportional bias (slope = 1.015, 95% CI: 1.000–1.032). HC-201 demonstrated excellent agreement (<i>ρ</i><sub>c</sub> = 0.989) and precision (ICC = 0.997) with a median bias of −0.67 g/L (<i>p</i> = 0.001). The total observed error was 3.02%, within the allowable limits defined by international standards. Neither anemia, leukocytosis, azotemia, lipemia, nor venipuncture site influenced HC-201 measurements. Samples with icterus and hemolysis were insufficient in number for statistical comparison.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>This preliminary study indicates that HC-201 offers reliable point-of-care monitoring for hemoglobin concentration in cats.</p>\n </section>\n </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 3","pages":"211-220"},"PeriodicalIF":1.1000,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12444007/pdf/","citationCount":"0","resultStr":"{\"title\":\"Method Comparison and Investigation of Interference Variables of a Hand-Held Hemoglobinometer(HemoCue Hb 201+) in Cats\",\"authors\":\"Yuqing Sun, Xiu Ting Yiew, Janet Beeler-Marfisi, Carolyn L. Kerr, Gabrielle Monteith, Shane Bateman\",\"doi\":\"10.1111/vcp.70035\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>Regular blood sampling to monitor RBC mass in anemic cats can exacerbate anemia. Laboratory-based reporting can delay clinical decisions. A hand-held hemoglobinometer, HemoCue Hb 201<sup>+</sup> (HC-201), requires only one drop of blood (10 μL) and provides results within 1 min.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Objectives</h3>\\n \\n <p>This preliminary study aimed to evaluate the utility of HC-201 in cats and investigate the impact of potential interferents on its performance.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>One hundred and fifty-four venous blood samples in EDTA from 93 cats were analyzed. Hemoglobin concentration was measured once using an ADVIA 2120 analyzer and compared to the mean of 2–3 replicate measurements from an HC-201. Agreement and systematic bias between HC-201 and ADVIA results, along with precision between HC-201 replicates, were assessed using Lin's concordance correlation coefficient, non-parametric Bland–Altman, Passing-Bablok regression, and intraclass correlation coefficient. The performance of HC-201 in the presence of anemia, leukocytosis, azotemia, lipemia, icterus, hemolysis, and peripheral versus jugular venipunctures was assessed using Wilcoxon rank-sum tests.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>Passing-Bablok analysis revealed a significant constant bias (intercept = −2.242, 95% CI: −4.042 to −0.667) but no significant proportional bias (slope = 1.015, 95% CI: 1.000–1.032). HC-201 demonstrated excellent agreement (<i>ρ</i><sub>c</sub> = 0.989) and precision (ICC = 0.997) with a median bias of −0.67 g/L (<i>p</i> = 0.001). The total observed error was 3.02%, within the allowable limits defined by international standards. Neither anemia, leukocytosis, azotemia, lipemia, nor venipuncture site influenced HC-201 measurements. Samples with icterus and hemolysis were insufficient in number for statistical comparison.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>This preliminary study indicates that HC-201 offers reliable point-of-care monitoring for hemoglobin concentration in cats.</p>\\n </section>\\n </div>\",\"PeriodicalId\":23593,\"journal\":{\"name\":\"Veterinary clinical pathology\",\"volume\":\"54 3\",\"pages\":\"211-220\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2025-07-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12444007/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary clinical pathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/vcp.70035\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary clinical pathology","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/vcp.70035","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Method Comparison and Investigation of Interference Variables of a Hand-Held Hemoglobinometer(HemoCue Hb 201+) in Cats
Background
Regular blood sampling to monitor RBC mass in anemic cats can exacerbate anemia. Laboratory-based reporting can delay clinical decisions. A hand-held hemoglobinometer, HemoCue Hb 201+ (HC-201), requires only one drop of blood (10 μL) and provides results within 1 min.
Objectives
This preliminary study aimed to evaluate the utility of HC-201 in cats and investigate the impact of potential interferents on its performance.
Methods
One hundred and fifty-four venous blood samples in EDTA from 93 cats were analyzed. Hemoglobin concentration was measured once using an ADVIA 2120 analyzer and compared to the mean of 2–3 replicate measurements from an HC-201. Agreement and systematic bias between HC-201 and ADVIA results, along with precision between HC-201 replicates, were assessed using Lin's concordance correlation coefficient, non-parametric Bland–Altman, Passing-Bablok regression, and intraclass correlation coefficient. The performance of HC-201 in the presence of anemia, leukocytosis, azotemia, lipemia, icterus, hemolysis, and peripheral versus jugular venipunctures was assessed using Wilcoxon rank-sum tests.
Results
Passing-Bablok analysis revealed a significant constant bias (intercept = −2.242, 95% CI: −4.042 to −0.667) but no significant proportional bias (slope = 1.015, 95% CI: 1.000–1.032). HC-201 demonstrated excellent agreement (ρc = 0.989) and precision (ICC = 0.997) with a median bias of −0.67 g/L (p = 0.001). The total observed error was 3.02%, within the allowable limits defined by international standards. Neither anemia, leukocytosis, azotemia, lipemia, nor venipuncture site influenced HC-201 measurements. Samples with icterus and hemolysis were insufficient in number for statistical comparison.
Conclusions
This preliminary study indicates that HC-201 offers reliable point-of-care monitoring for hemoglobin concentration in cats.
期刊介绍：
Veterinary Clinical Pathology is the official journal of the American Society for Veterinary Clinical Pathology (ASVCP) and the European Society of Veterinary Clinical Pathology (ESVCP). The journal''s mission is to provide an international forum for communication and discussion of scientific investigations and new developments that advance the art and science of laboratory diagnosis in animals. Veterinary Clinical Pathology welcomes original experimental research and clinical contributions involving domestic, laboratory, avian, and wildlife species in the areas of hematology, hemostasis, immunopathology, clinical chemistry, cytopathology, surgical pathology, toxicology, endocrinology, laboratory and analytical techniques, instrumentation, quality assurance, and clinical pathology education.
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