Effect of Storage at 4°C vs. 23°C–25°C on Complete Blood Counts in Dunkin Hartley Guinea Pigs

IF 1.1 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology Pub Date : 2025-07-25 DOI:10.1111/vcp.70031

Yan-Ni Huang, Yu-Han Xie, Jian-An Ling, Rui-Ze Chen, Song-Wen Fu, Jing Xie, Michael Aschner, Yue-Ming Jiang

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引用次数: 0

Abstract

Background

Dunkin Hartley guinea pig is a common guinea pig strain used by researchers, but studies about the stability of Dunkin Hartley guinea pig blood samples stored at different temperatures are limited.

Objectives

The study aimed to evaluate storage factors of complete blood count (CBC) in Dunkin Hartley guinea pigs.

Methods

We collected 2 mL blood samples from 20 Dunkin Hartley guinea pigs (weighted 250–300 g, 5–6 weeks, 10 males and 10 females) with 2 EDTA-K₂ vacuum tubes and performed CBC with a Hemavet 950FS analyzer at 0, 4, 8, 24, 48, 72, 96, and 120 h after storage at 4°C and 23°C–25°C, respectively. Analysis of the effect of the testing time, storage temperature, and sex on CBC was carried out by three-way repeated measures analysis of variance (RM-ANOVA).

Results

The results showed that white blood cells (WBCs), heterophil, lymphocyte, and monocyte counts, and red blood cells (RBCs) remained stable over time in 4°C storage. Only WBC, monocyte counts, and RBC remained stable over time with storage at 23°C–25°C. No differences were observed between 4°C and 23°C–25°C for WBCs and platelet count (PLT), but PLT was significantly increased from 96 h to 120 h at 4°C storage and at 72 h and 96 h at 23°C–25°C storage.

Conclusions

When CBC in Dunkin Hartley guinea pigs has to be delayed, storing samples at 4°C is preferred, and the storage time should not exceed 24 h.

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4℃与23℃-25℃贮藏对Dunkin Hartley豚鼠全血细胞计数的影响

背景：Dunkin Hartley豚鼠是研究人员常用的豚鼠品系，但关于Dunkin Hartley豚鼠血液样品在不同温度下储存稳定性的研究有限。目的：研究Dunkin Hartley豚鼠全血细胞计数（CBC）的储存因子。方法：用2根EDTA-K2真空管采集20只Dunkin Hartley豚鼠（体重250-300 g， 5-6周，雄性和雌性各10只）2 mL血样，分别于4℃和23℃-25℃保存后0、4、8、24、48、72、96和120 h，用Hemavet 950FS分析仪进行全血细胞计数。采用三向重复测量方差分析（RM-ANOVA）分析检测时间、储存温度和性别对CBC的影响。结果：结果显示，在4°C的储存中，白细胞（WBCs）、嗜白细胞、淋巴细胞和单核细胞计数以及红细胞（rbc）保持稳定。只有白细胞、单核细胞计数和红细胞在23°C-25°C保存期间保持稳定。在4°C和23°C-25°C之间，白细胞和血小板计数（PLT）没有差异，但PLT在4°C储存时从96 h增加到120 h，在23°C-25°C储存时从72 h增加到96 h。结论：当需要延迟Dunkin Hartley豚鼠CBC时，最好将样品保存在4℃，保存时间不应超过24 h。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary clinical pathology 农林科学-兽医学

CiteScore

1.70

自引率

16.70%

发文量

133

审稿时长

18-36 weeks

期刊介绍： Veterinary Clinical Pathology is the official journal of the American Society for Veterinary Clinical Pathology (ASVCP) and the European Society of Veterinary Clinical Pathology (ESVCP). The journal''s mission is to provide an international forum for communication and discussion of scientific investigations and new developments that advance the art and science of laboratory diagnosis in animals. Veterinary Clinical Pathology welcomes original experimental research and clinical contributions involving domestic, laboratory, avian, and wildlife species in the areas of hematology, hemostasis, immunopathology, clinical chemistry, cytopathology, surgical pathology, toxicology, endocrinology, laboratory and analytical techniques, instrumentation, quality assurance, and clinical pathology education.