Diagnostic Sensitivity of the PCR for Antigen Receptor Rearrangement (PARR) Assay for Canine Plasma Cell Tumors

IF 1.1 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology Pub Date : 2025-07-15 DOI:10.1111/vcp.70036

Emily D. Rout, Kacie Seymour, Robert Burnett, Cora Contreras, Anne C. Avery, A Russell Moore

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Abstract

Background

The PCR for Antigen Receptor Rearrangement (PARR) assay assesses clonality of a lymphoid population. Studies suggest PARR has decreased sensitivity for detecting clonality in canine plasma cell tumors (PCT) compared to lymphoma.

Objective

Assess sensitivity of PARR assays targeting the immunoglobulin heavy chain (IGH), immunoglobulin lambda light chain (IGL), and kappa deleting element (Kde) loci in canine PCT.

Methods

Canine cases included 35 PCTs (multiple myeloma and extramedullary, cutaneous or oral PCTs), 5 non-PCT foil cases, and 40 B-cell lymphoma cases. PCT diagnoses were confirmed via CD3, PAX5, and MUM1 immunolabeling, and some additionally had histopathology, serum or urine protein electrophoresis, or immunofixation. Nodal B-cell lymphomas were diagnosed by flow cytometry. Routine PARR (targeting IGH V-D-J and D-J rearrangements) and extended PARR (targeting additional IGH genes and IGL and Kde rearrangements) were performed on cytologic specimens (PCT and foil cases) and flow cytometry aspirates (lymphoma cases). Diagnostic sensitivity was calculated.

Results

Across both PARR assays, every PCT case with sufficient sample was interpreted as clonal and no foil cases were clonal. The routine PARR assay detected a clonal immunoglobulin rearrangement in 26/35 PCT cases (74.3%; 95% CI 56.7%–87.5%). The extended assay detected immunoglobulin gene clonality in 33/35 PCT cases (94.3%; 95% CI 80.8%–99.3%). 40/40 lymphoma cases were clonal in both the routine and extended PARR assays.

Conclusion

The combined PARR assays used in this study, evaluating multiple IG loci, detected clonality in all PCTs tested, but routine PARR (targeting IGH) was less sensitive for detecting PCT clonality compared to B-cell lymphomas.

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抗原受体重排（PARR） PCR检测犬浆细胞瘤的诊断敏感性

背景：抗原受体重排（PARR） PCR检测评估淋巴细胞群体的克隆性。研究表明，与淋巴瘤相比，PARR在犬浆细胞肿瘤（PCT）中检测克隆性的敏感性降低。目的：评价免疫球蛋白重链（IGH）、免疫球蛋白轻链（IGL）和卡帕删除元件（Kde）位点在犬pct中的敏感性。方法：35例犬pct（多发性骨髓瘤和髓外、皮肤或口腔pct）， 5例非pct foil病例和40例b细胞淋巴瘤。PCT诊断通过CD3、PAX5和MUM1免疫标记得到证实，部分患者另外进行组织病理学、血清或尿蛋白电泳或免疫固定。流式细胞术诊断淋巴结b细胞淋巴瘤。常规PARR（靶向IGH V-D-J和D-J重排）和扩展PARR（靶向额外的IGH基因和IGL和Kde重排）对细胞学标本（PCT和箔病例）和流式细胞术抽吸物（淋巴瘤病例）进行。计算诊断灵敏度。结果：在两种PARR分析中，每一个有足够样本的PCT病例都被解释为克隆，没有箔病例被解释为克隆。常规PARR检测在26/35例PCT病例中检测到克隆性免疫球蛋白重排(74.3%；95% ci 56.7%-87.5%)。扩展试验在33/35例PCT病例中检测到免疫球蛋白基因克隆(94.3%；95% ci 80.8%-99.3%)。40/40的淋巴瘤病例在常规和扩展PARR检测中均为克隆。结论：本研究中使用的联合PARR分析评估了多个IG位点，检测了所有PCT的克隆性，但与b细胞淋巴瘤相比，常规PARR（靶向IGH）检测PCT克隆性的敏感性较低。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary clinical pathology 农林科学-兽医学

CiteScore

1.70

自引率

16.70%

发文量

133

审稿时长

18-36 weeks

期刊介绍： Veterinary Clinical Pathology is the official journal of the American Society for Veterinary Clinical Pathology (ASVCP) and the European Society of Veterinary Clinical Pathology (ESVCP). The journal''s mission is to provide an international forum for communication and discussion of scientific investigations and new developments that advance the art and science of laboratory diagnosis in animals. Veterinary Clinical Pathology welcomes original experimental research and clinical contributions involving domestic, laboratory, avian, and wildlife species in the areas of hematology, hemostasis, immunopathology, clinical chemistry, cytopathology, surgical pathology, toxicology, endocrinology, laboratory and analytical techniques, instrumentation, quality assurance, and clinical pathology education.