Veterinary clinical pathology最新文献

{"title":"What Is Your Diagnosis? Severe Anemia in an Adult Alpaca.","authors":"Brittany N Martin, Ji Ann Greenberg, Pabasara Weerarathne, Guilherme Verocai, Dusty W Nagy, Mary B Nabity, Mark C Johnson","doi":"10.1111/vcp.70139","DOIUrl":"https://doi.org/10.1111/vcp.70139","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148840152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of a Commercially Available Canine C-Reactive Protein Assay Across Institutions and After 2 Years of Frozen Storage.","authors":"Marion Cambrelin, Amanda F Bettag, Lorraine E Prevost, A Russell Moore","doi":"10.1111/vcp.70147","DOIUrl":"https://doi.org/10.1111/vcp.70147","url":null,"abstract":"<p><strong>Background: </strong>C-reactive protein (CRP) is a biomarker of systemic inflammation in dogs. There is limited data on inter-laboratory comparability of the results or the effects of long-term storage.</p><p><strong>Objectives: </strong>(1) Compare CRP measurements across three institutions utilizing the same assay. (2) Evaluate 2-year stability of CRP in canine serum stored at -80°C.</p><p><strong>Methods: </strong>Canine serum was collected and pooled to obtain 100 samples, which were distributed among three veterinary clinical pathology laboratories and evaluated using the same commercially available canine CRP assay. Method comparison was performed according to current recommendations. A subset of samples was then stored at -80°C for 2 years and re-analyzed to assess long-term stability.</p><p><strong>Results: </strong>Passing-Bablok and Bland-Altman analyses revealed statistically significant constant and proportional bias between the three laboratories. However, the clinical impact was minimal (mean difference percentages were 4.0%-15.6% and mean difference absolute values were 0.9-4.0 mg/L), differences rarely exceeded the total allowable error (30%), and only two samples crossed the 10 mg/L inflammation decision limit. Similarly, re-testing after storage revealed a statistically significant increase in CRP concentration (mean difference of 0.75 mg/L, p < 0.001). However, only one sample exhibited a change greater than 30%, and the mean percentage change across all samples was 9.0%. Long-term storage did not cause any samples to cross the clinical inflammation decision limit.</p><p><strong>Conclusions: </strong>While there was statistical evidence of differences between laboratories and after long-term storage, it was unlikely to alter clinical interpretation or patient management.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148831885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Your Diagnosis? Bronchoalveolar Lavage From a Cat.","authors":"Lina C Bilhalva, Maxime G Derré, Kyra D Holt, Timothy A Bolton, Sriveny Dangoudoubiyam, Andrea P Santos","doi":"10.1111/vcp.70149","DOIUrl":"https://doi.org/10.1111/vcp.70149","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148819671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute Myeloid Leukemia With Suspected Basophilic Differentiation in a Cat Negative for Feline Leukemia Virus.","authors":"Manami Sato, Michihito Tagawa, Kohei Murakami, Nao Akiyama, Rina Nabeta","doi":"10.1111/vcp.70148","DOIUrl":"https://doi.org/10.1111/vcp.70148","url":null,"abstract":"<p><p>An 8-year-old spayed female domestic shorthair cat presented with anorexia and weight loss. A complete blood count revealed mild nonregenerative anemia, circulating blasts, dysplastic granulocytes, and basophilia. Both feline leukemia virus (FeLV) and feline immunodeficiency virus tested negative upon SNAP testing using peripheral blood and PCR using bone marrow. Bone marrow aspiration revealed an increased number of blast cells and basophils, and some blast cells were positive for MPO staining. Based on the basophilic lineages observed in the peripheral blood and bone marrow, acute myeloid leukemia with maturation (AML-M2) with basophilic differentiation was the most likely diagnosis in this case. This study reports the unique morphologic features of a rare case of AML-M2 with suspected basophilic differentiation in an FeLV-negative cat.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148808962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Hemoparasites and Ticks on Hematologic Response in Wild Black Spiny-Tailed Iguanas (Ctenosaura similis, Squamata: Iguanidae) From Costa Rica.","authors":"Randall Arguedas, Lizbeth Ovares, Idania Valverde, Allan Fernández, Daniel González, Marco D Barquero","doi":"10.1111/vcp.70146","DOIUrl":"https://doi.org/10.1111/vcp.70146","url":null,"abstract":"<p><strong>Background: </strong>Parasitism is widespread in Neotropical reptiles, yet hematologic variation remains poorly characterized for many species. The Black Spiny-tailed Iguana (Ctenosaura similis) commonly harbors both intracellular hemoparasites and ticks, but population-based hematologic data under natural field conditions are limited.</p><p><strong>Objectives: </strong>To characterize population-level hematologic values using descriptive hematologic intervals in free-ranging adult C. similis, and to evaluate associations between hematologic variables, intracellular hemoparasites, and tick burden.</p><p><strong>Methods: </strong>Blood samples were obtained from 89 free-ranging adult iguanas from two sites in Costa Rica. Hemoparasites were identified based on blood smear morphology. Ticks were removed from each animal, then counted and categorized by life stage. Hematologic variables included erythrogram, white blood cell (WBC) counts, thrombocyte counts, and total solids. Descriptive hematologic intervals were calculated using the robust method. Associations between parasitism and hematologic variables were evaluated using linear and multiple regression models.</p><p><strong>Results: </strong>Hemoparasite prevalence was high (93.3%), but hemoparasite loads were not significantly associated with erythrogram or WBC variables. In contrast, tick burden was associated with several factors. Hemoglobin concentration decreased with increasing numbers of adult ticks. Adult female ticks remained negatively associated with hemoglobin in adjusted models. WBC showed stage-specific relationships: heterophil-to-lymphocyte ratios increased with nymph abundance, and eosinophil counts increased with adult female ticks. All significant models had low explanatory power (R² ≤ 0.14).</p><p><strong>Conclusions: </strong>Tick burden, but not hemoparasite load, was associated with variation in hematologic variables in C. similis. These findings provide population-based hematologic context for this species and support cautious interpretation of hematologic data in parasitized reptiles.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148809038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Your Diagnosis? Blood Film From a 12.5-Year-Old Cat.","authors":"Elyssa L Armstrong, Rachel E Whitman, Kathryn H Shaffert, Elizabeth A Rozanski, Fábio Brum Rosa, Francisco O Conrado","doi":"10.1111/vcp.70143","DOIUrl":"https://doi.org/10.1111/vcp.70143","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148762309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fine-Needle Aspirate Cytology of Feline Pulmonary Langerhans Cell Histiocytosis in Two Cats.","authors":"Karuna Katariwala, Martim Matias, Isabel Amores-Fuster, Doroteia Bota, Guido Rocchigiani, Carolina Naranjo, Nazaré Pinto da Cunha, Martina Piviani","doi":"10.1111/vcp.70141","DOIUrl":"https://doi.org/10.1111/vcp.70141","url":null,"abstract":"<p><p>Feline pulmonary Langerhans cell histiocytosis (FPLCH) is a rare histiocytic disorder in cats, usually diagnosed post-mortem following natural death or euthanasia for severe respiratory distress. Antemortem diagnosis has been reported only once, achieved by cytologic examination of bronchoalveolar lavage fluid combined with immunocytochemistry, whereas fine-needle aspiration (FNA) of lung nodules was previously reported as non-diagnostic. Here we describe two cases of FPLCH in which cytology of ultrasound-guided lung-nodule FNA yielded diagnostic material, including from one cat with an atypical clinical presentation. Whereas case 1 presented with severe respiratory distress, case 2 had no respiratory signs. Both specimens were highly cellular and composed of atypical cells morphologically most consistent with histiocytes showing moderate atypia. A combination of postmortem gross, histopathologic, immunophenotypic, and ultrastructural findings confirmed the cytologic suspicion of FPLCH. To our knowledge, this is the first cytologic description of FPLCH in FNA samples from lung nodules and the first report of a confirmed case without respiratory signs.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148759412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Agreement of Hematologic Measurements Between Jugular and Coccygeal Puncture Sites in Cattle.","authors":"Sophie Clouard, Camille Remuzat, Thomas Chetot","doi":"10.1111/vcp.70144","DOIUrl":"10.1111/vcp.70144","url":null,"abstract":"<p><strong>Background: </strong>In bovine medicine, venipuncture is commonly performed at either the jugular or coccygeal vein. Although the coccygeal site is often preferred for practical reasons, sampling sites are inconsistently standardized in scientific studies. It is therefore important to determine whether hematologic values obtained from these two sites can be considered interchangeable.</p><p><strong>Objectives: </strong>To evaluate the statistical and clinical concordance of hematologic measurands obtained from paired jugular and coccygeal blood samples in cattle.</p><p><strong>Methods: </strong>Paired jugular and coccygeal blood samples were collected from 48 adult cows and analyzed using an automated hematology analyzer, micro-hematocrit determination, and blood smear evaluation. Agreement between sites was assessed using Pearson and Lin's correlation coefficients, Passing-Bablok regression, and Bland-Altman analysis, with clinical relevance evaluated against published total allowable error (TEa) thresholds.</p><p><strong>Results: </strong>Most hematologic measurands showed good agreement between jugular and coccygeal sampling sites. However, platelet counts, packed cell volume (PCV), and certain manual leukocyte differential counts on blood smears, particularly neutrophils and lymphocytes, demonstrated poorer agreement and should not be considered interchangeable.</p><p><strong>Conclusions: </strong>Jugular and coccygeal puncture sites yield largely comparable hematologic results for most automated measurands. However, platelet indices, packed cell volume, and manual leukocyte differentials on blood smears, particularly neutrophils and lymphocytes, show greater variability between sites and should not be considered interchangeable. In clinical settings, when results are compared with reference intervals or used for longitudinal monitoring, consistent use and documentation of the puncture site are recommended, and reference intervals established using the same sampling site should be applied for these variables.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148722336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Your Diagnosis? Abdominal Wall Mass in a Dog.","authors":"Morwenna Rodgers, Slavomira Necova, Cassia Hare","doi":"10.1111/vcp.70122","DOIUrl":"https://doi.org/10.1111/vcp.70122","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148707797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laboratory Changes Supporting a Diagnosis of Immune-Mediated Anemia in Cats With Initial Suspicion of Progressive Feline Leukemia Virus.","authors":"Nicholas Kelly, Katie McCallum, Will Bayton, Tyler Willard, Cassia Hare","doi":"10.1111/vcp.70137","DOIUrl":"https://doi.org/10.1111/vcp.70137","url":null,"abstract":"<p><strong>Background: </strong>Progressive feline leukemia virus (FeLV) infection and anemia of suspected immune-mediated origin (ASIMO) are associated with similar hematologic abnormalities, including anemia, neutropenia, and lymphocytosis. Cats with ASIMO may test positive for FeLV using point-of-care (POC) ELISA tests.</p><p><strong>Objective: </strong>To describe laboratory changes that may result in the misclassification of cats with ASIMO as having progressive FeLV.</p><p><strong>Methods: </strong>Cats with ASIMO and laboratory results mimicking FeLV infection were retrospectively identified via searches of laboratory and clinical records. Inclusion criteria were: 1) a positive POC FeLV result with negative confirmatory testing, 2) hematocrit < 20%, with or without macrocytosis, neutropenia, lymphocytosis, or suspicion of circulating atypical cells, and 3) a clinical diagnosis of ASIMO. Clinical and laboratory data, treatment, and outcome were recorded.</p><p><strong>Results: </strong>Nine cats met the inclusion criteria. All were vaccinated against FeLV. Seven (78%) had outdoor access and two (22%) were indoor only. Hematocrit at presentation ranged from 7-16%, and macrocytosis was recorded in seven cases (78%), of which six were normochromic by analyzer data. Seven cats (78%) had one or more laboratory indicators of hemolysis, including an abnormal RBC histogram in all five cases in which this was available for review. Other hematologic abnormalities included neutropenia (n = 6, 67%), lymphocytosis (n = 1, 11%), and circulating atypical cells (n = 1, 11%).</p><p><strong>Conclusions: </strong>Cats with ASIMO may present with macrocytic, normochromic anemia and other laboratory features mimicking progressive FeLV. Evaluation of RBC histograms alongside detailed blood film evaluation is helpful in identifying immune-mediated anemia as a likely diagnosis.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148702232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}