Veterinary clinical pathology最新文献

{"title":"HARISS: Histogram Analyzer for Reference Intervals of Small Samples, a Free Web App to Calculate Reference Intervals of Small Samples.","authors":"Kevin Le Boedec","doi":"10.1111/vcp.70033","DOIUrl":"https://doi.org/10.1111/vcp.70033","url":null,"abstract":"<p><strong>Background: </strong>Reference interval (RI) estimate inaccuracy is problematic at small sample sizes. Visual assessment of distribution histograms (VADH) may improve statistical technique selection, but its performance depends on the human operator.</p><p><strong>Objectives: </strong>This study aimed to develop a machine learning model for small-sample VADH.</p><p><strong>Methods: </strong>The training set consisted of 45 000 distribution histograms from samples ranging from 20 to 40 individuals extracted from simulated Gaussian, lognormal, and left-skewed populations. A convolutional neural network (CNN) was trained to predict the original population distribution by VADH. Its accuracy was tested on 900 human-classified histograms from a previous study (ranging from 20 to 60 individuals) and compared to the Shapiro-Wilk test performance in determining the original population distribution. A web application was developed to allow usage of the CNN, 95% RI estimation with 90% confidence intervals (CI) via bootstrapping, and outlier detection.</p><p><strong>Results: </strong>The CNN model properly predicted the original population distribution by VADH in 84.0% (95% CI: 83.7-84.4) and 94.4% (95% CI: 92.7-95.8) of the samples of the training and the test sets, respectively. Comparatively, on the test set, the Shapiro-Wilk test accuracy was 65.0% (95% CI: 61.8-68.1) and 72.3% (95% CI: 69.3-75.2) using a p-value threshold of 0.05 and 0.2, respectively. The web application (named HARISS) was successfully deployed and is accessible at: https://hariss.streamlit.app/.</p><p><strong>Conclusions: </strong>The CNN model demonstrated effective VADH and might enhance RI estimate accuracy through the use of the HARISS web app, though proper reference individual selection and preanalytical/analytical conditions remain paramount.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144627206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Reference Intervals for the TEG 6s, a Point-of-Care Viscoelastic Assay, in Dogs","authors":"Ashley R. Wilkinson,&nbsp;Bobbi J. Conner,&nbsp;Priscila B. S. Serpa","doi":"10.1111/vcp.70027","DOIUrl":"10.1111/vcp.70027","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Thromboelastography (TEG) provides a global assessment of hemostasis and identifies patients with both hemorrhagic and thrombotic tendencies. Traditional TEG instruments, such as the TEG 5000 (Haemonetics), can be labor-intensive and require specialized training to operate. The TEG 6s (Haemonetics) is a commercially available point-of-care TEG that employs a cartridge-based system. Agreement between the TEG 6s and TEG 5000 is poor in dogs, and their viscoelastic measurements are not interchangeable. Reference intervals specific to the TEG 6s are needed for healthy adult dogs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This study aimed to establish reference intervals for the TEG 6s in healthy adult dogs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Citrated whole blood samples collected from 40 healthy adult dogs were analyzed using the TEG 6s and associated global hemostasis and lysis cartridges. Reference intervals were established using a nonparametric statistical method for all variables except for the Functional Fibrinogen level in the global hemostasis cartridge.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Reference intervals were established for 12 TEG 6s measurands in healthy adult dogs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The results of this study provide reference intervals for TEG 6s variables associated with the global hemostasis and lysis cartridges in healthy adult dogs. Further studies are required to assess TEG 6s' utility in dogs with various disease states.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 3","pages":"292-299"},"PeriodicalIF":1.1,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12444008/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144627205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Stab in the Dark","authors":"Rachel E. Whitman,&nbsp;Francisco O. Conrado","doi":"10.1111/vcp.70045","DOIUrl":"10.1111/vcp.70045","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 2","pages":"75-77"},"PeriodicalIF":1.2,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144576416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical Reference Intervals of Free-Ranging Koalas (Phascolarctos cinereus) in South Australia","authors":"María B. Font,&nbsp;Lucy Woolford,&nbsp;Sue Jaensch,&nbsp;Doug Hayward,&nbsp;Michelle Hebart,&nbsp;Emily Dean,&nbsp;Wayne S. J. Boardman,&nbsp;Tamsyn Stephenson,&nbsp;Jessica Kovac,&nbsp;Natasha Speight","doi":"10.1111/vcp.70024","DOIUrl":"10.1111/vcp.70024","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Reference intervals (RIs) are an essential tool for assessment of clinical pathology data of animals, and are particularly important for monitoring the health status of free-ranging and captive wildlife, such as koalas (<i>Phascolarctos cinereus</i>).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>The purpose of this study was to: (1) provide comprehensive serum biochemistry reference intervals based on clinically healthy South Australian koalas from two populations, Mount Lofty Ranges (MLR) and Kangaroo Island (KI); and (2) identify any factors that can affect biochemical analytes, including koala retrovirus (KoRV) and <i>Chlamydia pecorum</i> subclinical infection status, age, sex, and population.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Serum biochemistry analytes were determined in 206 clinically healthy South Australian koalas caught from the wild in 2016 and 2018 using a Cobas 8000 Chemistry Analyzer and analyzed using Reference Value Advisor and SPSS v28 Statistical software.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Biochemical reference intervals were established. Also, clinically and statistically significant differences in analytes were found based on age for alkaline phosphatase and phosphate, and albumin: globulin ratio, globulins, and total protein, most likely associated with physiological bone growth and immunological development, respectively, as observed in other species. Statistically significant differences between animals subclinically positive for KoRV and <i>Chlamydia pecorum</i>, were found for glucose and gamma glutamyl transferase respectively; however, these were marginal, and their reference intervals were similar.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>This study is the first to describe serum biochemical reference intervals for clinically healthy South Australian koalas of known <i>Chlamydia</i> and KoRV infection status. It represents an important tool to assist health assessments of koalas by veterinarians, as well as research and population monitoring.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 3","pages":"300-308"},"PeriodicalIF":1.1,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12444011/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144545031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transient Leukemia in an Adult Dog With Cutaneous T-Cell Lymphoma","authors":"Raquel Moreira,&nbsp;Malgorzata Ossowska,&nbsp;Oriol Jornet-Rius,&nbsp;Marta Santos,&nbsp;Francesco Cian","doi":"10.1111/vcp.70025","DOIUrl":"10.1111/vcp.70025","url":null,"abstract":"<p>A 5-year-old neutered female Beagle was presented with lethargy, vomiting, and reduced appetite. Physical examination revealed mild hyperthermia and a cutaneous thickness on the right thigh. Hematology showed marked leukocytosis and moderate thrombocytopenia, consisting of 80% of atypical circulating cells, initially suggesting acute undifferentiated leukemia. Two weeks later, repeated hematology revealed unremarkable results. Cytology of the skin lesion showed a round cell neoplasia with features similar to the atypical circulating cells. Following the development of multiple cutaneous nodules and recurrence of clinical signs, further diagnostics, including flow cytometry, histopathology, and immunohistochemistry (IHC) of the skin nodules, as well as PCR for antigen receptor rearrangement (PARR) and immunocytochemistry (ICC) from the initial blood smear, confirmed a neoplastic T-cell proliferation consistent with cutaneous T-cell lymphoma with a probable transient leukemic phase. Despite chemotherapy, remission was short-lived, and the patient relapsed, ultimately leading to euthanasia. This case highlights a rare instance of transient leukemia likely originating from a primary cutaneous T-cell lymphoma, emphasizing the need for comprehensive diagnostic workups, combining hematology, biochemistry, cytology, flow cytometry, and immunophenotyping to avoid misclassification in hematologic malignancies.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 3","pages":"264-270"},"PeriodicalIF":1.1,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12444006/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Hemostatic Activity in Canine Leukoreduced Cryoprecipitate, Cryopoor Plasma, and Fresh Plasma","authors":"Roberta Perego,&nbsp;Eva Spada,&nbsp;Luciana Baggiani,&nbsp;Giuliano Ravasio,&nbsp;Enrica Zucca,&nbsp;Graziella Vanosi,&nbsp;Giancarlo Ruffo,&nbsp;Daniela Proverbio","doi":"10.1111/vcp.70017","DOIUrl":"10.1111/vcp.70017","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>To date, no studies have reported the evaluation of hemostatic activity in canine leukoreduced cryoprecipitate (LR-CRYO) and leukoreduced cryopoor plasma (LR-CPP).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>We aimed to compare the hemostatic activity of LR-CRYO and LR-CPP to leukoreduced fresh plasma (LR-FP) and to evaluate the preservation of LR-CRYO by refrigeration and refreezing after thawing.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Four hundred fifty milliliters of fresh blood was collected from ten donor dogs, leukoreduced, and separated into LR-FP, then frozen (−20°C) to obtain leukoreduced fresh frozen plasma (LR-FFP). LR-FFP was further separated into LR-CRYO and LR-CPP. LR-CRYO was frozen, thawed, and divided into two bags, one refrigerated for 24 h and one refrozen for 7 days. Factor VIII (FVIII) and X (FX) activity, prothrombin time, activated partial thromboplastin time, antithrombin III (ATIII) activity (ATA), total protein, albumin, fibrinogen, and D-dimer concentration, and von Willebrand Factor (vWF) activity were measured in LR-FP, LR-CRYO, LR-CPP, refrigerated, and refrozen LR-CRYO.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>FVIII activity was higher in LR-CRYO (<i>p</i> = 0.0001) versus LR-FP. vWF activity (<i>p</i> &lt; 0.0001) and fibrinogen concentration (<i>p</i> = 0.0012) were lower in LR-CPP versus LR-FP. FX activity was higher in LR-CPP (<i>p</i> &lt; 0.0001) and LR-FP (<i>p</i> = 0.0002) versus LR-CRYO, and albumin concentration was higher in LR-CPP versus LR-FP (<i>p</i> &lt; 0.0001) and LR-CRYO (<i>p</i> &lt; 0.0001). No statistically significant difference was found in refrigerated or refrozen LR-CRYO as compared with LR-CRYO, excluding ATA, which was lower (<i>p</i> = 0.0062) in refrigerated LR-CRYO.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Because the concentration of FVIII is higher in LR-CRYO than in LR-FP, LR-CRYO is a possible component therapy when this factor is deficient. Since no statistically significant difference was found in refrozen LR-CRYO as compared with LR-CRYO, LR-CRYO can be frozen after thawing for reuse.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 2","pages":"120-131"},"PeriodicalIF":1.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.70017","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unusual Cytologic Findings in a Dog With a Keratinizing Cyst and Cutaneous Asthenia","authors":"Jeremy P. Bessett,&nbsp;Kimberley N. Sebastian","doi":"10.1111/vcp.70019","DOIUrl":"10.1111/vcp.70019","url":null,"abstract":"<p>A 5-year-old spayed female golden retriever with a history of cutaneous asthenia (Ehlers-Danlos-like syndrome) was presented with a newly noted large hindlimb mass. Cytologic evaluation performed at the referring veterinarian was consistent with a keratinizing lesion. However, a second cytologic sample was obtained from the mass, aspirating from multiple aspects, and was concerning for a sarcoma. The mass was excised and submitted for histologic evaluation. The mass effect was diagnosed as a keratinizing cyst. Additionally, there were severe dermal collagen and elastin abnormalities with multifocal fibrosis and hemorrhage (consistent with cutaneous asthenia). To the best of the authors' knowledge, this is the first report of the cytologic findings in the skin of a canine with cutaneous asthenia.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 2","pages":"182-185"},"PeriodicalIF":1.2,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.70019","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144286611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Your Diagnosis: Mediastinal Mass in a Dog","authors":"Jelena Palić,&nbsp;Jaco Jan van der Lugt,&nbsp;Karin Troedson,&nbsp;Helena Nyman Lee","doi":"10.1111/vcp.70028","DOIUrl":"10.1111/vcp.70028","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 S1","pages":"S37-S40"},"PeriodicalIF":1.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Value of Neutrophil Cell Population Data Parameters as Markers of Systemic Inflammation in Dogs and Cats","authors":"Dylan S. O′ Toole,&nbsp;Tim L. Williams,&nbsp;Cassia H. Z. Hare","doi":"10.1111/vcp.70029","DOIUrl":"10.1111/vcp.70029","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Neutrophil cell population data (CPD), including fluorescent light intensity (NE-SFL) and side scatter (NE-SSC), are promising inflammatory markers in human sepsis but remain unexplored in dogs and cats.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Determine the diagnostic utility of NE-SSC and NE-SFL for detecting systemic inflammation in dogs and cats.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Dogs and cats with archived CPD, blood films, and acute phase protein (APP) measurements were included. Increased C-reactive protein (CRP) in dogs and Serum Amyloid A (SAA) in cats were considered indicative of systemic inflammation. CPD was compared with APPs, white cell count (WCC), neutrophil count, band neutrophil count, and toxic change grade. Optimal cut-offs and associated sensitivities and specificities were calculated using ROC curve analysis. Correlations were assessed using Spearman's coefficient.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>NE-SFL and NE-SSC were significantly increased in dogs and cats with systemic inflammation. The area under the curve (AUC) of NE-SFL was higher than that of NE-SSC, WCC, and band neutrophil count in both dogs (0.82) and cats (0.77). The optimal NE-SFL cut-off for detecting systemic inflammation was &gt; 41.7 ch in dogs (sensitivity 80%; specificity 66%) and &gt; 37.4 ch in cats (sensitivity 75%; specificity 67%). NE-SFL was positively correlated with APPs, WCC, neutrophil count, and band neutrophil count in both species. NE-SSC was positively correlated with APPs in both species and, in dogs, also with WCC, neutrophil count, and band neutrophil count.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>CPD, particularly NE-SFL, is a promising marker of inflammation in dogs and cats and could be especially useful when APP quantification or blood smear examination are unavailable.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 2","pages":"78-86"},"PeriodicalIF":1.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.70029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144235369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two Point-of-Care Cardiac Troponin I Immunoassays Have Acceptable Analytical Performance for the Detection of Measurands of Cardiac Troponin I Cardiac Muscle Homogenates From Southern-Central Black Rhinoceros (Diceros bicornis minor) and Southern White Rhinoceros (Ceratotherium simum simum)","authors":"Yolandi Rautenbach,&nbsp;Leith C. R. Meyer,&nbsp;Amelia Goddard,&nbsp;Peter E. Buss,&nbsp;Emma H. Hooijberg","doi":"10.1111/vcp.70016","DOIUrl":"10.1111/vcp.70016","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Skeletal and possible cardiac muscle damage has been reported in chemically immobilized and transported African rhinoceros during conservation-related activities. The extent of cardiac muscle injury in these rhinoceros is unknown due to a lack of validated cardiac troponin I (cTnI) assays. However, recently, five human cTnI assays were deemed suitable for analytical validation in African rhinoceros based on cTnI sequencing results.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>The first objective was to validate two cTnI immunoassay point-of-care analyzers (POCAs) in African rhinoceros and, secondly, to perform quality control (QC) validation for the POCAs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Analytical validation of the Stratus CS Acute Care Troponin I cTnI immunoassay and Atellica VTLi high sensitivity cTnI (hs-cTnI) assay was performed using rhinoceros serum samples and species-specific cardiac muscle homogenate. Experiments included precision studies, reportable range, hemoglobin interference studies, recovery studies, and detection limit studies, with results assessed against prescribed total allowable error (TE_a) performance goals. Commercial quality control material (QCM) data were used to calculate bias and imprecision for QC validation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Imprecision was acceptable (1.9%–10.3%) and met low cTnI concentration performance goals. Reportable ranges were similar to the manufacturer's specifications. High hemoglobin concentrations in white rhinoceros resulted in a positive bias in the Stratus CS. A simple 1_3s QC rule using two levels of QCM and a TEa of 70% could be used in both analyzers, except at very low cTnI concentrations in the Atellica VTLi.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Both cTnI POCAs are suitable for use in African rhinoceros, and analytical performance goals for low cTnI concentrations in hs-cTnI assays were met.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 2","pages":"142-153"},"PeriodicalIF":1.2,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.70016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}