The Value of Neutrophil Cell Population Data Parameters as Markers of Systemic Inflammation in Dogs and Cats

IF 1.1 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology Pub Date : 2025-06-06 DOI:10.1111/vcp.70029

Dylan S. O′ Toole, Tim L. Williams, Cassia H. Z. Hare

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引用次数: 0

Abstract

Background

Neutrophil cell population data (CPD), including fluorescent light intensity (NE-SFL) and side scatter (NE-SSC), are promising inflammatory markers in human sepsis but remain unexplored in dogs and cats.

Objectives

Determine the diagnostic utility of NE-SSC and NE-SFL for detecting systemic inflammation in dogs and cats.

Methods

Dogs and cats with archived CPD, blood films, and acute phase protein (APP) measurements were included. Increased C-reactive protein (CRP) in dogs and Serum Amyloid A (SAA) in cats were considered indicative of systemic inflammation. CPD was compared with APPs, white cell count (WCC), neutrophil count, band neutrophil count, and toxic change grade. Optimal cut-offs and associated sensitivities and specificities were calculated using ROC curve analysis. Correlations were assessed using Spearman's coefficient.

Results

NE-SFL and NE-SSC were significantly increased in dogs and cats with systemic inflammation. The area under the curve (AUC) of NE-SFL was higher than that of NE-SSC, WCC, and band neutrophil count in both dogs (0.82) and cats (0.77). The optimal NE-SFL cut-off for detecting systemic inflammation was > 41.7 ch in dogs (sensitivity 80%; specificity 66%) and > 37.4 ch in cats (sensitivity 75%; specificity 67%). NE-SFL was positively correlated with APPs, WCC, neutrophil count, and band neutrophil count in both species. NE-SSC was positively correlated with APPs in both species and, in dogs, also with WCC, neutrophil count, and band neutrophil count.

Conclusion

CPD, particularly NE-SFL, is a promising marker of inflammation in dogs and cats and could be especially useful when APP quantification or blood smear examination are unavailable.

Abstract Image

查看原文本刊更多论文

中性粒细胞群体数据参数作为狗和猫全身炎症标志物的价值。

背景：中性粒细胞群数据（CPD），包括荧光光强度（NE-SFL）和侧散射（NE-SSC），是人类败血症中有希望的炎症标志物，但在狗和猫中仍未被探索。目的：确定NE-SSC和NE-SFL在犬猫全身性炎症检测中的诊断价值。方法：包括存档的CPD、血片和急性期蛋白（APP）测量的狗和猫。狗的c反应蛋白（CRP）和猫的血清淀粉样蛋白A （SAA）升高被认为是全系统炎症的指示。比较CPD与app、白细胞计数（WCC）、中性粒细胞计数、带中性粒细胞计数、毒性变化分级。使用ROC曲线分析计算最佳截止点和相关的灵敏度和特异性。使用Spearman系数评估相关性。结果：NE-SFL和NE-SSC在全身性炎症的狗和猫中显著升高。NE-SFL的曲线下面积（AUC）在狗（0.82）和猫（0.77）中均高于NE-SSC、WCC和带状中性粒细胞计数。在犬中，NE-SFL检测全身性炎症的最佳临界值为41.7 ch(敏感性80%；特异性66%)和> 37.4 ch(敏感性75%；特异性67%)。NE-SFL与两种动物的APPs、WCC、中性粒细胞计数和带状中性粒细胞计数呈正相关。在两种动物中，NE-SSC与APPs呈正相关，在狗中，也与WCC、中性粒细胞计数和带状中性粒细胞计数呈正相关。结论：CPD，特别是NE-SFL，是一种很有希望的狗和猫炎症标志物，在APP量化或血液涂片检查不可用时特别有用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary clinical pathology 农林科学-兽医学

CiteScore

1.70

自引率

16.70%

发文量

133

审稿时长

18-36 weeks

期刊介绍： Veterinary Clinical Pathology is the official journal of the American Society for Veterinary Clinical Pathology (ASVCP) and the European Society of Veterinary Clinical Pathology (ESVCP). The journal''s mission is to provide an international forum for communication and discussion of scientific investigations and new developments that advance the art and science of laboratory diagnosis in animals. Veterinary Clinical Pathology welcomes original experimental research and clinical contributions involving domestic, laboratory, avian, and wildlife species in the areas of hematology, hemostasis, immunopathology, clinical chemistry, cytopathology, surgical pathology, toxicology, endocrinology, laboratory and analytical techniques, instrumentation, quality assurance, and clinical pathology education.