Comparison of Hemostatic Activity in Canine Leukoreduced Cryoprecipitate, Cryopoor Plasma, and Fresh Plasma

IF 1.1 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology Pub Date : 2025-06-16 DOI:10.1111/vcp.70017

Roberta Perego, Eva Spada, Luciana Baggiani, Giuliano Ravasio, Enrica Zucca, Graziella Vanosi, Giancarlo Ruffo, Daniela Proverbio

{"title":"Comparison of Hemostatic Activity in Canine Leukoreduced Cryoprecipitate, Cryopoor Plasma, and Fresh Plasma","authors":"Roberta Perego, Eva Spada, Luciana Baggiani, Giuliano Ravasio, Enrica Zucca, Graziella Vanosi, Giancarlo Ruffo, Daniela Proverbio","doi":"10.1111/vcp.70017","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>To date, no studies have reported the evaluation of hemostatic activity in canine leukoreduced cryoprecipitate (LR-CRYO) and leukoreduced cryopoor plasma (LR-CPP).</p>\n </section>\n \n <section>\n \n <h3> Objectives</h3>\n \n <p>We aimed to compare the hemostatic activity of LR-CRYO and LR-CPP to leukoreduced fresh plasma (LR-FP) and to evaluate the preservation of LR-CRYO by refrigeration and refreezing after thawing.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Four hundred fifty milliliters of fresh blood was collected from ten donor dogs, leukoreduced, and separated into LR-FP, then frozen (−20°C) to obtain leukoreduced fresh frozen plasma (LR-FFP). LR-FFP was further separated into LR-CRYO and LR-CPP. LR-CRYO was frozen, thawed, and divided into two bags, one refrigerated for 24 h and one refrozen for 7 days. Factor VIII (FVIII) and X (FX) activity, prothrombin time, activated partial thromboplastin time, antithrombin III (ATIII) activity (ATA), total protein, albumin, fibrinogen, and D-dimer concentration, and von Willebrand Factor (vWF) activity were measured in LR-FP, LR-CRYO, LR-CPP, refrigerated, and refrozen LR-CRYO.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>FVIII activity was higher in LR-CRYO (<i>p</i> = 0.0001) versus LR-FP. vWF activity (<i>p</i> < 0.0001) and fibrinogen concentration (<i>p</i> = 0.0012) were lower in LR-CPP versus LR-FP. FX activity was higher in LR-CPP (<i>p</i> < 0.0001) and LR-FP (<i>p</i> = 0.0002) versus LR-CRYO, and albumin concentration was higher in LR-CPP versus LR-FP (<i>p</i> < 0.0001) and LR-CRYO (<i>p</i> < 0.0001). No statistically significant difference was found in refrigerated or refrozen LR-CRYO as compared with LR-CRYO, excluding ATA, which was lower (<i>p</i> = 0.0062) in refrigerated LR-CRYO.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>Because the concentration of FVIII is higher in LR-CRYO than in LR-FP, LR-CRYO is a possible component therapy when this factor is deficient. Since no statistically significant difference was found in refrozen LR-CRYO as compared with LR-CRYO, LR-CRYO can be frozen after thawing for reuse.</p>\n </section>\n </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 2","pages":"120-131"},"PeriodicalIF":1.1000,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.70017","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary clinical pathology","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/vcp.70017","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Background

To date, no studies have reported the evaluation of hemostatic activity in canine leukoreduced cryoprecipitate (LR-CRYO) and leukoreduced cryopoor plasma (LR-CPP).

Objectives

We aimed to compare the hemostatic activity of LR-CRYO and LR-CPP to leukoreduced fresh plasma (LR-FP) and to evaluate the preservation of LR-CRYO by refrigeration and refreezing after thawing.

Methods

Four hundred fifty milliliters of fresh blood was collected from ten donor dogs, leukoreduced, and separated into LR-FP, then frozen (−20°C) to obtain leukoreduced fresh frozen plasma (LR-FFP). LR-FFP was further separated into LR-CRYO and LR-CPP. LR-CRYO was frozen, thawed, and divided into two bags, one refrigerated for 24 h and one refrozen for 7 days. Factor VIII (FVIII) and X (FX) activity, prothrombin time, activated partial thromboplastin time, antithrombin III (ATIII) activity (ATA), total protein, albumin, fibrinogen, and D-dimer concentration, and von Willebrand Factor (vWF) activity were measured in LR-FP, LR-CRYO, LR-CPP, refrigerated, and refrozen LR-CRYO.

Results

FVIII activity was higher in LR-CRYO (p = 0.0001) versus LR-FP. vWF activity (p < 0.0001) and fibrinogen concentration (p = 0.0012) were lower in LR-CPP versus LR-FP. FX activity was higher in LR-CPP (p < 0.0001) and LR-FP (p = 0.0002) versus LR-CRYO, and albumin concentration was higher in LR-CPP versus LR-FP (p < 0.0001) and LR-CRYO (p < 0.0001). No statistically significant difference was found in refrigerated or refrozen LR-CRYO as compared with LR-CRYO, excluding ATA, which was lower (p = 0.0062) in refrigerated LR-CRYO.

Conclusions

Because the concentration of FVIII is higher in LR-CRYO than in LR-FP, LR-CRYO is a possible component therapy when this factor is deficient. Since no statistically significant difference was found in refrozen LR-CRYO as compared with LR-CRYO, LR-CRYO can be frozen after thawing for reuse.

Abstract Image

查看原文本刊更多论文

犬白细胞诱导的低温沉淀、低温血浆和新鲜血浆止血活性的比较。

背景：迄今为止，尚未有研究报道犬白细胞诱导低温沉淀（LR-CRYO）和白细胞诱导低温血浆（LR-CPP）的止血活性评估。目的：比较LR-CRYO和LR-CPP对白细胞诱导新鲜血浆（LR-FP）的止血活性，并评价冷冻和解冻后再冷冻对LR-CRYO的保存效果。方法：取10只供犬新鲜血液450 ml，经白细胞诱导，分离成LR-FP，冷冻（-20℃），得到白细胞诱导的新鲜冷冻血浆（LR-FFP）。LR-FFP进一步分离为LR-CRYO和LR-CPP。LR-CRYO冷冻、解冻后分成两袋，一袋冷藏24小时，一袋再冷冻7天。测定LR-FP、LR-CRYO、LR-CPP、冷藏和再冷冻LR-CRYO中凝血因子VIII （FVIII）和X （FX）活性、凝血酶原时间、活化部分凝血酶时间、抗凝血酶III （ATIII）活性（ATA）、总蛋白、白蛋白、纤维蛋白原和d -二聚体浓度以及血管性血友病因子（vWF）活性。结果：与LR-FP相比，LR-CRYO的FVIII活性更高（p = 0.0001）。结论：由于FVIII在LR-CRYO中的浓度高于LR-FP，因此当该因子缺乏时，LR-CRYO可能是一种成分治疗方法。由于再冷冻LR-CRYO与再冷冻LR-CRYO无统计学差异，因此解冻后可以再次冷冻LR-CRYO。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Veterinary clinical pathology 农林科学-兽医学

CiteScore

1.70

自引率

16.70%

发文量

133

审稿时长

18-36 weeks

期刊介绍： Veterinary Clinical Pathology is the official journal of the American Society for Veterinary Clinical Pathology (ASVCP) and the European Society of Veterinary Clinical Pathology (ESVCP). The journal''s mission is to provide an international forum for communication and discussion of scientific investigations and new developments that advance the art and science of laboratory diagnosis in animals. Veterinary Clinical Pathology welcomes original experimental research and clinical contributions involving domestic, laboratory, avian, and wildlife species in the areas of hematology, hemostasis, immunopathology, clinical chemistry, cytopathology, surgical pathology, toxicology, endocrinology, laboratory and analytical techniques, instrumentation, quality assurance, and clinical pathology education.