Veterinary clinical pathology最新文献

{"title":"What Is Your Diagnosis? Blood Smear From a Kitten","authors":"Erin E. Farrell, Renata M. Mammone, Gayle C. Johnson, Ji-Hey Lim, Margret L. Casal, Angela B. Royal","doi":"10.1111/vcp.70011","DOIUrl":"10.1111/vcp.70011","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 S1","pages":"S33-S36"},"PeriodicalIF":1.1,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Veterinary Laboratory Quality, Basic Principles and Selected Topics, 1st Edition. By Kathleen Freeman, Stefanie Klenner-Gastreich, and Jeremie Korchia, Madison, WI, USA: Westgard QC, 2024. 376 pp. $80 (paperback). ISBN: 978-1-886958-39-5","authors":"Simone Manzocchi","doi":"10.1111/vcp.70010","DOIUrl":"https://doi.org/10.1111/vcp.70010","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 2","pages":"191-192"},"PeriodicalIF":1.2,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What's Your Diagnosis? Dog With a Laryngeal Mass","authors":"Benjamin J. Haythornthwaite,&nbsp;Dylan Yaffy,&nbsp;Emma J. Holmes","doi":"10.1111/vcp.70014","DOIUrl":"10.1111/vcp.70014","url":null,"abstract":"<p>An 8-year, 3-month-old female, entire Boxer dog presented to the Queen Mother Hospital for Animals (QMHA), Royal Veterinary College (RVC) for evaluation of increased upper airway noise and dysphagia. A laryngeal mass had been identified by the referring veterinarian 5 months previously while the dog was undergoing general anesthetic for removal of oral masses (confirmed as peripheral odontogenic fibromas). At this time, no clinical signs were associated with the mass. However, in the weeks prior to presentation, the dog became unable to tolerate solid food and started showing respiratory symptoms relating to the mass and was subsequently referred for evaluation. CT examination revealed a 35 × 28 × 39 mm soft tissue mass occupying the right side of the rima glottidis, displacing the laryngeal lumen. The mass extended from the level of the rostral margin of the tympanic bulla to the level of C1 and dorsally into the caudal aspect of the nasopharynx. Direct aspirate preparations (Figures 1 and 2) from the laryngeal mass were air-dried, fixed, and stained using modified Wright stain (Hema-tek 3000, Siemens).</p><p>Incisional biopsy samples obtained at the same time as the aspirates were fixed in formalin, routinely processed, and stained with H&amp;E.</p><p>Sections had a densely cellular pleomorphic malignant neoplasm, composed of diffuse sheets of irregularly polygonal to elongate cells of two populations. The more prominent population had large amounts of vacuolated cytoplasm with lower numbers of smaller round cells with moderate amounts of finely granular eosinophilic cytoplasm. Most cells had a single irregularly round nucleus with one prominent large nucleolus. Anisocytosis and anisokaryosis of the larger cells were marked, with frequent multinucleated giant cells and 57 bizarre mitotic figures in 2.37 mm² (Figure 3). Atypia of the smaller cell population was mild. The histological interpretation was an atypical malignant neoplasm with differentials including rhabdomyoma/sarcoma, oncocytoma, granular cell tumor, amelanotic melanoma, or histiocytic sarcoma.</p><p>Histochemical staining revealed strong cytoplasmic positivity for periodic acid-Schiff (PAS) in the small cells but negativity in the larger cells, diffuse strong membranous, and cytoplasmic positivity for vimentin in approximately 95% of cells (Figure 4), diffuse moderate cytoplasmic positivity for desmin in approximately 80% of cells, and negativity for toluidine blue, phosphotungstic acid hematoxylin (PTAH), cytokeratin, ionized calcium-binding adaptor molecule 1 (Iba-1), melan-A, smooth muscle actin (α-sma), neuron-specific enolase (NSE), and S100 (Table 1).</p><p>Based on these staining characteristics and cellular pleomorphism, a diagnosis of rhabdomyosarcoma was made.</p><p>Laryngeal rhabdomyosarcoma is mainly reported in adult dogs, being locally invasive but rarely metastatic. This local invasion makes complete excision difficult, with tumor recurrence often resu","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 S1","pages":"S41-S44"},"PeriodicalIF":1.1,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.70014","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial Intelligence in Veterinary Clinical Pathology-An Introduction and Review.","authors":"Samuel V Neal, Daniel G Rudmann, Kara N Corps","doi":"10.1111/vcp.70012","DOIUrl":"https://doi.org/10.1111/vcp.70012","url":null,"abstract":"<p><p>Artificial intelligence (AI), particularly through machine learning and deep learning, presents opportunities for the enhancement of the workflow of the veterinary clinical pathologist. This review introduces basic concepts in AI in a nontechnical manner and explores the qualification and integration of AI in veterinary clinical pathology. The veterinary clinical pathologist must play an active role in defining the intended use, design, and qualification of these methods as well as the plan for monitoring their responsible application in practice.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the influence of hepatic triglyceride and lipoprotein lipases on lipase assays used to diagnose pancreatitis in dogs and cats","authors":"Hiroki Harada,&nbsp;Masahiro Tamura,&nbsp;Kumi Ishii,&nbsp;Tatsuyuki Osuga,&nbsp;Hiroshi Ohta","doi":"10.1111/vcp.70002","DOIUrl":"10.1111/vcp.70002","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Pancreas-specific lipase and lipase activity measured by immunological assays (Spec cPL and Spec fPL) and colorimetric assays (1,2-<i>o</i>-dilauryl-<i>rac</i>-glycelo-3-glutaric acid-(6'-methylresorufin) ester [DGGR] and triolein), respectively, are used to diagnose pancreatitis in both dogs and cats. However, DGGR and triolein assays may be influenced by extrapancreatic lipases, including hepatic triglyceride lipase (HTGL) and lipoprotein lipase (LPL).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>To investigate the effect of extrapancreatic lipases on immunological and colorimetric assays by measuring changes in HTGL and LPL activity following heparin administration.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Six healthy Beagles and six adult purpose-bred cats were enrolled. HTGL and LPL activities were induced by intravenous heparin administration. Serum samples were collected at baseline and at 5-, 10-, 15-, and 60-min following heparin injection. Spec cPL, Spec fPL, and lipase activities were measured using DGGR and triolein assays, whereas HTGL and LPL activities were measured using their respective assays.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Spec cPL and Spec fPL levels showed no significant changes following heparin administration. Conversely, DGGR-based and triolein-based lipase activities, as well as HTGL and LPL activities, were significantly increased after heparin administration in both dogs and cats. HTGL and LPL activities showed significant positive correlations with DGGR-based (<i>P</i> &lt; .001, <i>r</i> = .90 for both) and triolein-based (<i>P</i> &lt; .001, <i>r</i> = .63 and <i>P</i> &lt; .001, <i>r</i> = .68, respectively) lipase activities, but not with Spec cPL and Spec fPL.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>DGGR- and triolein-based lipase activities are influenced by HTGL and LPL activities, as their substrates are hydrolyzed by pancreatic lipase, HTGL, and LPL.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 2","pages":"132-141"},"PeriodicalIF":1.2,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of blood erythroid parameters in male broiler chickens (Ross 308) with the Sysmex XT-2000iV and Sysmex XN-1000V analyzers and determination of hematological reference intervals obtained with manual and instrumental methods","authors":"Damien Legroux,&nbsp;Laura Kersten,&nbsp;Gaelle Barral,&nbsp;Alexane Mauras,&nbsp;Thierry Buronfosse,&nbsp;Eve Ramery","doi":"10.1111/vcp.70009","DOIUrl":"10.1111/vcp.70009","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The presence of both nucleated RBC and thrombocytes in birds makes the determination of hematological variables difficult. This leads to time-consuming manual evaluation. New-generation analyzers are hypothesized to be both time-saving and accurate.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>The objective was to evaluate the performance of two hematology analyzers in 27-day-old broilers and to establish reference intervals (RIs) for RBC and WBC variables obtained by automated and manual methods.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Automated HCT, WBC count, and differentials obtained using Sysmex XT-2000iV and XN-1000V on 309 and 56 EDTA blood samples from Ross 308 male broilers, respectively, were compared to manual PCV, manual WBC count, and differentials from November 2021 to November 2022. RIs for automated HCT and RBC variables (from impedance channel) on one hand, and manual PCV and WBC's variables on the other hand, were established.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The Sysmex XT-2000iV was accurate for HCT and outperformed the XN-1000V but, both automated methods and manual methods cannot be considered interchangeable. Constant error, proportional error, and bias were identified and calculated for each analyzer for HCT. Both analyzers were unable to enumerate WBC. RIs were established as follows: HCT-XT (L/L) [0.27–0.36]; HCT-XN (L/L) [0.24–0.38]; manual PCV (L/L) [0.27–0.37]; manual WBC (×10⁹/L) [10.0–38.4]; manual differential (×10⁹/L) with heterophils [1.5–16.2]; lymphocytes [5.1–18.9]; monocytes [0.0–2.1]; eosinophils [0.0–2.2]; basophils [0.0–3.1].</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Both analyzers are usable for the evaluation of HCT in Ross 308 chickens and in specific conditions for the other RBC variables. Manual methods are still preferred for the evaluation of WBC variables.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 2","pages":"106-119"},"PeriodicalIF":1.2,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.70009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical occurrence of trypanosomiasis in Arabian horses from Ahvaz","authors":"Ali Abbas Nikvand,&nbsp;Seyedeh Missagh Jalali,&nbsp;Somayeh Bahrami,&nbsp;Hassan Rahij Torfi","doi":"10.1111/vcp.70003","DOIUrl":"10.1111/vcp.70003","url":null,"abstract":"<p><i>Trypanosoma evansi</i> (<i>T. evansi</i>) is a hemoprotozoan parasite affecting camels and equids, such as horses, mules, and donkeys, and is known to cause surra disease in these animals. Despite the worldwide distribution of <i>T. evansi</i> infections in equids, surra has not been reported in Arabian horses in Khuzestan Province for over 60 years. In September 2018, a 7-year-old Arabian mare was referred from a 10-horse farm in the suburbs of Ahvaz City. The mare presented with a history of weight loss, poor appetite, and proximity to a camel herd. Physical examination revealed a poor body condition score (BCS ≤ 3/9), pale conjunctival and oral mucosal membranes, dehydration, tachycardia, and fever. Other horses in the herd also exhibited gradual weight loss and hyporexia, with two fatalities reported. A hematologic examination of the referred mare showed leukopenia, severe anemia, and thrombocytopenia. Microscopic examination of a whole blood smear confirmed severe infection with <i>Trypanosoma</i>. PCR assessment of a blood sample further confirmed the infection with <i>T. evansi</i>. The referred mare and seven nonreferred patients from the herd received treatment with diminazene diaceturate (5 mg/kg, a single dose) and penicillin for 4 days. Unfortunately, the referred mare died despite the treatment, while the remaining nonreferred horses recovered. This report marks the first molecular diagnosis of surra in an Arabian horse in Khuzestan Province, highlighting the need for further investigation into surra as a re-emerging disease in this significant population of Arabian horses in the region.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 2","pages":"186-190"},"PeriodicalIF":1.2,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What is your diagnosis? Right frontal lobe mass in a dog.","authors":"Erin E Farrell, Dae Young Kim, Samantha D Crist, Gayle C Johnson, Wendy E Karnia","doi":"10.1111/vcp.70005","DOIUrl":"https://doi.org/10.1111/vcp.70005","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What's your diagnosis? Globular to amorphous material in peripheral blood smears.","authors":"Em Adam, Craig Thompson, Andrea Pires Dos Santos, Joanne Messick","doi":"10.1111/vcp.70008","DOIUrl":"https://doi.org/10.1111/vcp.70008","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypophosphatemia in Intensive Care Unit Canine Patients: Occurrence and Association With Mortality and Duration of Hospitalization","authors":"Ioannis L. Oikonomidis,&nbsp;Paul Rees,&nbsp;Jorge Hernando Sanz,&nbsp;Glynn Woods","doi":"10.1111/vcp.70013","DOIUrl":"10.1111/vcp.70013","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Hypophosphatemia is commonly observed in unselected human intensive care unit (ICU) patients, and it has been associated, although inconsistently, with worse outcomes and longer duration of hospitalization (DOH). The incidence of hypophosphatemia and its association with mortality and DOH in ICU canine patients is unknown.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>The aims of this study were to determine the occurrence of hypophosphatemia in unselected ICU canine patients and its association with mortality and DOH.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The medical records of all dogs admitted to the Teaching Hospital ICU between January 2019 and December 2023 were retrospectively reviewed. Dogs with hypophosphatemia (serum phosphate &lt; 0.9 mmol/L) were identified. For every hypophosphataemic dog included in this study, two age-matched control, non-hypophosphataemic dogs, closely admitted to the ICU in time, were included.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>In total, 3233 medical records were reviewed. Hypophosphatemia was noted in ≥ 1 day of hospitalization in 32 dogs (0.99%). The age-matched case and control groups had a median (range) age of 8.0 (1.0–14.0) years. The survival to discharge rates of both hypophosphataemic and control groups were 78% (25/32 and 50/64, respectively), and their DOH (median, 3.5 days; range 1.0–9.0 and median 3.0 days; range 1.0–25.0, respectively) were not significantly different (<i>p</i> = 0.557). Serum phosphate concentration was not correlated with the DOH (<i>p</i> = 0.649).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Hypophosphatemia was noted in only 1% in this canine ICU patient population and was not associated with the survival to discharge and DOH.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 2","pages":"167-170"},"PeriodicalIF":1.2,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.70013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144200148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}