Veterinary clinical pathology最新文献

{"title":"Life-Stage-Specific Hematological Profiles in Yellow Mealworm Beetles (Tenebrio molitor): Reference Intervals and Hemocyte Characterization.","authors":"Simon Maheu, Houda Ben-Miled, Dominique Marullo-Masson, Colin Favret, Carolyn Gara-Boivin, Marie-Odile Benoit-Biancamano","doi":"10.1111/vcp.70116","DOIUrl":"https://doi.org/10.1111/vcp.70116","url":null,"abstract":"<p><strong>Background: </strong>The edible insect industry, driven by the nutritional and environmental benefits of mealworms, faces increasing challenges from pathogens affecting Tenebrio molitor farming systems. Hemocyte-based health assessments could provide valuable diagnostic insights, yet standardized hemolymph processing protocols and hematological reference intervals (RIs) are currently lacking for this species.</p><p><strong>Objectives: </strong>This study aimed to develop practical protocols for hemolymph sampling, refine hemocyte classification, and establish hematological RIs for both larval and adult stages of T. molitor.</p><p><strong>Methods: </strong>An optimized tearing method, involving capillary collection of hemolymph from a proleg transected at the femoral-tibial joint, was used to sample healthy individuals. Total and differential hemocyte counts were determined using a hemocytometer and Wright-Giemsa-stained smears, respectively, and from distinct insect populations (n = 41-43 samples per group). Hemolymph was analyzed in pools due to the small size of T. molitor. Transmission electron microscopy was conducted on a multinucleated giant hemocyte (MGH) isolated from a histologic slide. RIs were calculated following American Society for Veterinary Clinical Pathology (ASVCP) guidelines, and life-stage differences were statistically assessed.</p><p><strong>Results: </strong>Granular cells and plasmatocytes predominated, whereas prohemocytes and oenocytoids were rare. MGHs, newly identified in T. molitor, were characterized as a fifth hemocyte population. Significant life-stage differences were observed, warranting separate RIs for larvae and adults. The established RIs were relatively wide, reflecting natural fluctuations in invertebrate hemolymph volume and the unsexed, age-diverse reference population.</p><p><strong>Conclusions: </strong>This report of hematological RIs for T. molitor lays the groundwork for implementing hemocyte-based health surveillance in yellow mealworm beetle farming.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148593362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Your Diagnosis? Coelomic Mass in a Parrot Cichlid (Amphilophus citrinellus × Paraneetroplus synspilus).","authors":"Angela Jackson, Sarah K Cudd, Elise E B LaDouceur","doi":"10.1111/vcp.70127","DOIUrl":"https://doi.org/10.1111/vcp.70127","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148593462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Your Diagnosis? Pulmonary Mass in a Cat.","authors":"Carrie George, Irene U Dobles, Anamarie Weber, Daniela Hernandez Muguiro","doi":"10.1111/vcp.70125","DOIUrl":"https://doi.org/10.1111/vcp.70125","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148593454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Case Report: T-Lymphoblastic Leukemia in a Dog With Erythrophagia.","authors":"Francisca B Lang, Matheus Y Dos Santos, Vinicius N Hirata, Felipe A B de Sousa, Augusto de Oliveira, Lucas T de Castro, Rafael A Fighera, Alexandre Krause","doi":"10.1111/vcp.70117","DOIUrl":"https://doi.org/10.1111/vcp.70117","url":null,"abstract":"<p><p>A 2-year-old male German Shepherd dog was presented with progressive weight loss, lethargy, and hyporexia. Hematologic analysis revealed a moderate macrocytic normochromic anemia and moderate thrombocytopenia. Additionally, a pronounced leukocytosis was observed, characterized by 33% large lymphoid cells, marked neutrophilia with a significant left shift, and intense lymphocytosis and monocytosis. These large lymphoid cells exhibited moderately basophilic cytoplasm and nuclei with chromatin loss and occasional prominent nucleoli, along with erythrophagia and both typical and atypical mitotic figures. Serum chemistry showed mild hypoproteinemia and hypoalbuminemia. Bone marrow aspirate revealed high cellularity with lymphoid predominance and 40.6% blasts per 1000 nucleated cells, of which 2.6% showed erythrophagia. PCR for antigen receptor rearrangements performed on DNA extracted from the bone marrow smear confirmed a clonal T-cell receptor rearrangement. Despite supportive treatment and initial chemotherapy, no clinical recovery was observed, and the patient was euthanized 8 days post-diagnosis. Necropsy revealed marked splenohepatomegaly, intensely red bone marrow, mesenteric lymphadenomegaly, gastric and duodenal ulcers, and jejunal intussusception. Histologically, the bone marrow was hypercellular with monomorphic neoplastic blast cells, some exhibiting erythrophagia. Neoplastic infiltration was observed in the liver, spleen, lymph nodes, and adrenal glands. Immunohistochemistry showed blast cells positive for CD3 and negative for CD20. The final diagnosis was T-lymphoblastic leukemia with erythrophagia, underscoring the diagnostic importance of hematologic, histopathologic, and immunohistochemical evaluations in diseases with nonspecific clinical signs. Furthermore, the uncommon presentation of erythrophagia by neoplastic cells may be associated with severe anemia and poor prognosis in this patient.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148563187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of ADVIA Myeloperoxidase Cytogram LUC Angle Does Not Distinguish Acute Myeloid Leukemia From Acute Lymphoblastic Leukemia or Lymphoblastic Lymphoma in Dogs.","authors":"Mayzie Miller, Nora L Springer, Dorothee Bienzle, Kristina Meichner, Tracy Stokol","doi":"10.1111/vcp.70134","DOIUrl":"https://doi.org/10.1111/vcp.70134","url":null,"abstract":"<p><strong>Background: </strong>Acute myeloid leukemia (AML) is difficult to distinguish from lymphoblastic leukemia or lymphoma (LL) on morphologic features alone. Therapeutic approaches for AML versus LL differ, and immediate therapy is often needed due to patient illness and rapid disease progression. Immunophenotyping is required for the distinction of AML from LL but is often not rapidly available. Hence, a routinely available method for distinguishing AML from LL at the time of diagnosis, such as myeloperoxidase (MPX) activity in tumor cells, is desirable.</p><p><strong>Objectives: </strong>To determine whether MPX activity on the ADVIA peroxidase cytogram differentiates AML vs LL, as judged by a lower angle of a line intersecting the large unstained cell (LUC) cluster and the x-axis on the ADVIA 2120 MPX cytogram (MPX LUC angle).</p><p><strong>Methods: </strong>Dogs with suspect acute leukemia were prospectively enrolled, and ADVIA-based hematology, flow cytometry, and cytochemical staining were performed in a multi-institutional study. An observer blinded to sample identity determined the MPX LUC angle with Fiji software.</p><p><strong>Results: </strong>Forty-four cases met the inclusion criteria and were grouped as AML (n = 30) or LL (n = 14). No significant difference in the mean MPX LUC angle was identified between AML and LL (p = 0.9). MPX cytochemical staining did not correlate with MPX LUC angle (Rsp = 0.004, p > 0.9).</p><p><strong>Conclusions: </strong>The angle of the LUC population on the ADVIA MPX cytogram cannot be used as a rapid test to differentiate AML from LL.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148563194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of an Immunoturbidometric Assay for Measurement of Total Adiponectin Concentration in Equine Serum Samples.","authors":"Rose D Nolen-Walston, Caitrin R Lowndes, Jeaneen C Kulp, Daniela Luethy, Darko Stefanovski, Andrew W Van Eps","doi":"10.1111/vcp.70118","DOIUrl":"https://doi.org/10.1111/vcp.70118","url":null,"abstract":"<p><strong>Background: </strong>Total adiponectin (TA) is a marker of metabolic health in equids, with values below a critical decision point of < 7.9 μg/mL conferring increased risk of laminitis.</p><p><strong>Objectives: </strong>To evaluate an IT assay to measure serum [TA] in equids and to assess its correlation with [high molecular weight adiponectin] (HMWA) measured by ELISA.</p><p><strong>Methods: </strong>Total serum adiponectin concentrations from 207 adult horses were measured using both an IT assay for TA and HMWA using a previously validated ELISA assay. Data were analyzed using Spearman correlation and Passing-Bablok regression. Intra- and inter-assay precision, linearity, lower limit of quantification (LLoQ), and freeze-thaw stability were assessed for the [TA] assay.</p><p><strong>Results: </strong>Mean (range) intra-assay coefficient of variation (CV%) was 2.3% (1.8%-2.8%); inter-assay, across-day CV% was 3.3% (3.25%-3.4%), and across reagent lot CV was 11.7% (9.0%-17.9%). Mean recovery upon dilution was 133.7% (122.6%-140.6%), and LLoQ 1.3 μg/mL. The median CV for 5 freeze-thaw cycles was 3.0% (range 1.0%-3.0%) with a median change in concentration of -11% (range -[9.9-17.1]%). A strong correlation between [TA] and [HMWA] is observed (ρ = 0.90 [95% confidence interval 0.86-0.92], p < 0.001), although in 18% of samples, [HMWA] > [TA].</p><p><strong>Conclusions: </strong>The turbidimetric immunoassay is sufficiently precise and reliable for measurement of [TA] in equids, especially around the critical decision point, and LLoQ was below the lowest clinical cut-off value. [TA] overall correlated well with [HMWA] in this population, although instances of higher [HMWA] than [TA] were noted at high [HMWA].</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148563137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the IDEXX Procyte Dx Analyzer for the Measurement of Total and Differential Cellular Counts of Bronchoalveolar Lavage Fluid in Healthy Beagles and Dogs with Lower Respiratory Tract Diseases.","authors":"Hélène Machiels, Aline Fastrès, Tom Biénès, Aurélie Lyssens, Alexandra Schlachet, Alexandru-Cosmin Tutunaru, Kim-Thu Phan, Frédéric Billen, Cécile Clercx, Elodie Roels","doi":"10.1111/vcp.70132","DOIUrl":"https://doi.org/10.1111/vcp.70132","url":null,"abstract":"<p><strong>Background: </strong>Bronchoalveolar lavage fluid (BALF) analysis is a key diagnostic tool in veterinary respiratory medicine. The IDEXX ProCyte Dx analyzer was developed for blood formulation and cellular counting in various fluids, but its applicability for BALF analysis remains unexplored.</p><p><strong>Objectives: </strong>To assess the agreement between manual counting and automated counting using the IDEXX ProCyte Dx analyzer for total cell count (TCC) and differential cell count (DCC) in BALF samples from healthy and diseased dogs, under two pretreatment conditions: mucolytic treatment with dithiothreitol (DTT) or filtration.</p><p><strong>Methods: </strong>BALF from 10 healthy beagles and 14 diseased dogs were analyzed to compare TCC and DCC between manual and automated methods across pretreatment conditions.</p><p><strong>Results: </strong>In healthy dogs, Bland-Altman analysis on log-transformed data demonstrated a mean ratio close to 1 and acceptable 95% limits of agreement between manual and automated methods and between pretreatment conditions. In diseased dogs, good agreement was observed between manual and automated methods for filtered samples only; the other comparisons showed poor agreement. In both healthy and diseased dogs, Passing-Bablok regression analysis did not detect systematic or proportional bias between methods. Automated DCC was inconsistent compared with manual methods in both healthy and diseased dogs.</p><p><strong>Conclusions: </strong>The IDEXX ProCyte Dx analyzer can be used for TCC measurement of pre-treated BALF samples in healthy dogs. In diseased dogs, filtration is preferred over DTT-treatment for automated counting, and results should be interpreted with caution due to the unpredictable influence of pretreatment on cellular counts. For DCC, automated methods are unreliable.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148550774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Your Diagnosis? Abnormal WNR Scattergram From the Sysmex XN-1000V in a Dog.","authors":"Javier Martínez-Caro, Marta Lemos, Albert Lloret, Josep Pastor","doi":"10.1111/vcp.70103","DOIUrl":"https://doi.org/10.1111/vcp.70103","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148449722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cerebrospinal Fluid Creatine Kinase as a Biomarker in Dogs With Neurologic Disease: A Multivariate Analysis to Guide Future Directions.","authors":"Taylor A Hanson, Michael F Rosser, Benjamin Blair, Graeme M Doodnaught, Chandler C Carter, Anne M Barger, Kari Foss","doi":"10.1111/vcp.70126","DOIUrl":"https://doi.org/10.1111/vcp.70126","url":null,"abstract":"<p><strong>Background: </strong>Creatine kinase (CK) is typically associated with skeletal muscle injury, but elevated CK in blood or cerebrospinal fluid (CSF) may also be reflective of central nervous system damage. Previous research has therefore explored the role of CK as a biomarker of neurologic disease in veterinary patients. However, the literature regarding this biomarker is somewhat incomplete, as control groups or reference populations have not always been included in previous studies.</p><p><strong>Objectives: </strong>This study aimed to further evaluate CSF CK in neurologic dogs-with a healthy control group for comparison-to investigate its predictive value for disease etiology and its relationship to neuroanatomic localization of disease, CSF collection site, other CSF parameters, and patient demographics.</p><p><strong>Animals: </strong>The control group included nine healthy Beagles. The neurologic cohort was comprised of 95 dogs with neurologic disease requiring CSF analysis.</p><p><strong>Methods: </strong>CSF was collected from appropriate sites with CK and routine parameters measured. A linear-mixed effects model analyzed relationships between variables.</p><p><strong>Results: </strong>There was no significant difference in CSF CK between normal and neurologic dogs as a whole. No neurologic etiology differed significantly from other groups or from healthy controls except for dogs with metabolic disease (n = 2). Age and CSF total protein were significant predictors of CSF CK, but no significant relationship was identified between CSF CK and neuroanatomic localization or other parameters.</p><p><strong>Conclusions: </strong>We suspect that CSF CK is of limited utility in diagnostic differentiation or localization of neurologic disease in dogs. We suggest that future research explore alternative applications of this biomarker.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148424651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Your Diagnosis? Skin Scrape Cytology From a Laboratory-Housed Female African Clawed Frog (Xenopus laevis).","authors":"Wencke Reineking, David K Chu, Kerriann M Casey, Flavio H Alonso","doi":"10.1111/vcp.70120","DOIUrl":"https://doi.org/10.1111/vcp.70120","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148424616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}