Veterinary clinical pathology最新文献_第7页

Correction to "ASVCP Guidelines: Principles of Quality Assurance and Standards for Veterinary Clinical Pathology (Version 3.0)". 更正“ASVCP指南：兽医临床病理学质量保证原则和标准（3.0版）”。

IF 1 4区农林科学

Veterinary clinical pathology Pub Date : 2026-06-23 DOI: 10.1111/vcp.70130

引用次数: 0

Kinetics of Regenerating Protein 3E and C-Reactive Protein in Healthy Dogs, Dogs Undergoing Tibial Plateau Leveling Osteotomy, and Dogs With Sepsis. 健康犬、胫骨平台平截骨犬和脓毒症犬的再生蛋白3E和c反应蛋白动力学

IF 1.1 4区农林科学

Veterinary clinical pathology Pub Date : 2026-06-12 DOI: 10.1111/vcp.70115

Laura Gentilini, Johanna Rompf, Judith Howard, Simone Schuller, Laureen M Peters

{"title":"Kinetics of Regenerating Protein 3E and C-Reactive Protein in Healthy Dogs, Dogs Undergoing Tibial Plateau Leveling Osteotomy, and Dogs With Sepsis.","authors":"Laura Gentilini, Johanna Rompf, Judith Howard, Simone Schuller, Laureen M Peters","doi":"10.1111/vcp.70115","DOIUrl":"https://doi.org/10.1111/vcp.70115","url":null,"abstract":"Background: Serial measurements of acute-phase proteins help monitor the development of and recovery from sepsis in people and dogs. To date, the kinetics of regenerating protein (REG) 3E, a novel biomarker for inflammation in dogs, are unknown.Objectives: The goal of our study was to assess changes in the concentrations of REG3E and compare these to C-reactive protein (CRP) concentrations over 48-72 h in different cohorts of dogs.Methods: Plasma REG3E concentrations were measured using an ELISA on 3-4 consecutive days from 43 dogs, including 12 healthy controls, 17 undergoing tibial plateau leveling osteotomy (TPLO), and 14 with sepsis. Canine CRP was measured using a turbidimetric immunoassay.Results: REG3E concentrations were low in control and TPLO dogs throughout all time points. In the TPLO group, there was a mild but significant increase in CRP over time. REG3E and CRP concentrations were higher in septic dogs at admission compared to the other groups and progressively decreased after initiation of treatment to 14% and 28% of initial levels, respectively, within 72 h. REG3E, but not CRP concentrations, stayed high in two dogs, one of which developed recurrent sepsis. There was a significant moderate correlation between both acute-phase proteins among all groups and within the sepsis group, but not within the TPLO group.Conclusions: In conclusion, based on this cohort of dogs, CRP appears to be more sensitive to detect minor postsurgical inflammation, but the potential of REG3E to predict developing or recurrent sepsis deserves further investigation.","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148228472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of Haemonetics TEG 6s Reference Interval in Healthy Dogs. 健康犬血液学TEG 6s参考区间的建立。

IF 1.1 4区农林科学

Veterinary clinical pathology Pub Date : 2026-05-19 DOI: 10.1111/vcp.70104

Kaylan Whetstone, Justin Heinz, Lonnie E Grantham, Michaela Stavropoulos, Chelsie Hall, Floris C Dröes, Thomas H Edwards

{"title":"Establishment of Haemonetics TEG 6s Reference Interval in Healthy Dogs.","authors":"Kaylan Whetstone, Justin Heinz, Lonnie E Grantham, Michaela Stavropoulos, Chelsie Hall, Floris C Dröes, Thomas H Edwards","doi":"10.1111/vcp.70104","DOIUrl":"https://doi.org/10.1111/vcp.70104","url":null,"abstract":"Background: A thromboelastogram viscoelastic monitor (the TEG 6s) has recently become available for use in veterinary patients. It is therefore necessary to establish reference intervals for the new device in each species where it is employed. This study aimed to establish accurate and acceptable reference intervals for the TEG 6s in dogs.Objective: The goal of this study was to establish dog reference intervals (RIs) for the TEG 6s by sampling healthy adult dogs from a variety of breeds.Methods: One hundred and thirty dogs (130) aged 1-9 years old were enrolled in this study. Inclusion criteria included healthy adult dogs with no previously reported coagulopathies, no medications that are known to alter coagulation, and normal CBC, biochemistry, and physical exam. TEG 6s measurements were performed using a Haemonetics Global Hemostasis cartridge and citrated blood sample.Results: One hundred and twenty-three (123) dogs were included in the TEG 6s RIs. The determined RIs for the citrated kaolin activated were reaction time (R) 2.1-5.6 min, kinetic time (K) 1.1-2.5 min, alpha angle 64.6°-74.9°, and maximum amplitude 47.3-62.0 mm. The determined RIs for citrated Rapid TEG (CRT) maximum amplitude and reaction for the kaolin-heparinase activated were 40.0-59.3 mm and 2.1-5.6 min, respectively.Conclusions: RIs were established in a large group of normal dogs, which can assist small animal veterinarians with the interpretation of a newer viscoelastic testing method.","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147975547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of Stability for Blood Urea Nitrogen in Cattle Serum Samples Stored for 3 and 6 Months at -20°C and -80°C. 在-20°C和-80°C下保存3个月和6个月的牛血清样品中尿素氮稳定性的丧失

IF 1.1 4区农林科学

Veterinary clinical pathology Pub Date : 2026-05-17 DOI: 10.1111/vcp.70114

I Lizarraga, P Reid, K Wieliczko, J Beeler-Marfisi, F Castillo-Alcala

{"title":"Loss of Stability for Blood Urea Nitrogen in Cattle Serum Samples Stored for 3 and 6 Months at -20°C and -80°C.","authors":"I Lizarraga, P Reid, K Wieliczko, J Beeler-Marfisi, F Castillo-Alcala","doi":"10.1111/vcp.70114","DOIUrl":"https://doi.org/10.1111/vcp.70114","url":null,"abstract":"Background: Biological samples are stored for delayed or add-on analyses. However, storage conditions, including time and temperature, can affect the stability of measurands in the samples.Objectives: To assess the stability of blood urea nitrogen (BUN) in cattle serum samples.Methods: Blood samples collected from 72 heifers were immediately analyzed for BUN by a commercial laboratory and our in-house laboratory (optimal conditions, t0). In-house, samples were also analyzed after storage at -20°C and -80°C for 3 and 6 months (test samples, tx). Agreement between laboratories was assessed using Passing-Bablok and Bland-Altman analyses. The maximum permissible instability (MPI = 4%) was calculated as the sum of in-house intra- and inter-assay analytical variation. Percentage deviation (PD% = [(tx-t0)/t0] × 100) was estimated for each frozen sample. Stability was assessed by comparing individual PD% against the MPI.Results: Our in-house analytical methodology performed within the ASVCP recommended guidelines. BUN (mmol/L) ranged from 1.9 to 5.4 in the commercial laboratory and 1.6 to 6.1 in our in-house laboratory. There was poor agreement with proportional bias in which the higher the BUN concentration, the greater the difference between laboratories. Storage at -20°C and -80°C yielded PD% between -18.6% and 27.1% after 3 months and -17.5% and 71.6% after 6 months. Regardless of freezing temperature, 54% and 80% of PD% values were greater than the MPI after 3- and 6-month's storage, respectively.Conclusions: Although cattle serum sample degradation begins by three months storage time, further studies are needed to establish the actual stability limit for BUN.","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147964879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Small-Sample Reference Interval Estimation: A Comparative Analysis of HARISS, Robust, Parametric, and Nonparametric Methods Using a Computer-Simulation Study. 小样本参考区间估计：HARISS、鲁棒、参数和非参数方法在计算机模拟研究中的比较分析。

IF 1.1 4区农林科学

Veterinary clinical pathology Pub Date : 2026-05-13 DOI: 10.1111/vcp.70110

Kevin Le Boedec

{"title":"Small-Sample Reference Interval Estimation: A Comparative Analysis of HARISS, Robust, Parametric, and Nonparametric Methods Using a Computer-Simulation Study.","authors":"Kevin Le Boedec","doi":"10.1111/vcp.70110","DOIUrl":"https://doi.org/10.1111/vcp.70110","url":null,"abstract":"Background: Current veterinary guidelines for establishing reference intervals (RIs) from small samples typically recommend the robust method (RM) or, alternatively, parametric or nonparametric methods (PNPM) based on data distribution (Gaussian or not). A web application, HARISS, refines this approach by using visual inspection of distribution histograms to more accurately select the statistical method for small samples, based on a 3-class classification of data distribution (Gaussian, lognormal, left-skewed). Prior simulation studies have shown HARISS's decision rules lead to more accurate RIs, assuming Gaussian, lognormal, or left-skewed population distributions.Objectives: This study aimed to compare the accuracy of HARISS, RM, and PNPM in estimating RIs when small samples are drawn from populations with diverse distributions.Methods: We randomly selected samples of n = 40, 50, or 60 values (50 replicates per size) from seven simulated populations (5000 values each): Gaussian, Student's t, lognormal, right-skewed, left-skewed, bimodal, and irregular. No outlier was included. RIs were constructed using HARISS, RM, and PNPM. Their accuracy was compared using repeated-measures ANOVA.Results: Overall, HARISS significantly improved the accuracy of both RI lower and upper limit estimations (p < 0.001), irrespective of population distribution or sample size. However, the methods' accuracy varied depending on the specific population distribution. Specifically, HARISS significantly outperformed RM for RIs derived from Gaussian and Student's t population samples and was significantly more accurate than PNPM for RIs from skewed population samples.Conclusions: Overall, HARISS provided the most accurate RI limit estimations from small samples across the seven population distributions simulated.","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147933758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

What Is Your Diagnosis? Blood Smear From a Mixed-Breed Dog. 你的诊断是什么？混血狗的血液涂片。

IF 1.1 4区农林科学

Veterinary clinical pathology Pub Date : 2026-05-08 DOI: 10.1111/vcp.70111

Laura S Peres, Lina C Bilhalva, Camila B E Mello, Abílio G Silva, Andrea P Santos, Stella F Valle

引用次数: 0

Identification of Lupus Erythematosus Cells and Ragocytes in Peripheral Blood from a Dog with Systemic Lupus Erythematosus. 系统性红斑狼疮犬外周血中红斑狼疮细胞和巨噬细胞的鉴定。

IF 1.1 4区农林科学

Veterinary clinical pathology Pub Date : 2026-05-07 DOI: 10.1111/vcp.70087

Samantha L Braman, Jeremy P Bessett, Katie L Anderson, Kristen R Friedrichs

引用次数: 0

Infection with the Neorickettsial Organism Stellantchasmus falcatus Agent in an Arctic Fox (Vulpes lagopus). 北极狐（Vulpes lagopus）感染新立克氏体菌。

IF 1.1 4区农林科学

Veterinary clinical pathology Pub Date : 2026-05-04 DOI: 10.1111/vcp.70109

Jeremy P Bessett, Alexis G Davidson, Alyssa M Scagnelli, Kristen R Friedrichs

{"title":"Infection with the Neorickettsial Organism Stellantchasmus falcatus Agent in an Arctic Fox (Vulpes lagopus).","authors":"Jeremy P Bessett, Alexis G Davidson, Alyssa M Scagnelli, Kristen R Friedrichs","doi":"10.1111/vcp.70109","DOIUrl":"https://doi.org/10.1111/vcp.70109","url":null,"abstract":"A 1-year-old female-intact arctic fox (Vulpes lagopus) presented for diarrhea, lethargy, severe thrombocytopenia, and hyperbilirubinemia. Blood film evaluation revealed intracytoplasmic coccoid bacteria within moderate numbers of leukocytes consistent with infection by a rickettsial organism. Similar organisms were observed within many leukocytes from fine-needle aspirates of an enlarged peripheral lymph node. The lymph node also contained moderate necrosis and an expansion of granular lymphocytes. Parasitic ovum consistent with the trematode Nanophyetus salmincola was found on fecal floatation. The patient responded to treatment with doxycycline, enrofloxacin, ampicillin/sulbactam, and other supportive therapies. Polymerase chain reaction testing confirmed the presence of Neorickettsia sp., and DNA sequencing identified the organism as Stellantchasmus falcatus agent. To the authors' knowledge, this is the first report describing this organism microscopically in peripheral blood, the first reported case of infection in this species of canid, and describes the various clinicopathologic changes with this infection.","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Persistent Erythrocytosis in a Dog With a Spinal Sclerosing Paraganglioma. 犬脊髓硬化副神经节瘤的持续性红细胞增多症。

IF 1.1 4区农林科学

Veterinary clinical pathology Pub Date : 2026-05-03 DOI: 10.1111/vcp.70112

Samuel V Neal, Samantha L Sommer, Sarah Golconda, Eric T Hostnik, Russell Sakacs, Janice Park, Ashley Potts, Ronaldo C da Costa, Christopher Premanandan, Kara Corps, Sanggu Kim, Maxey L Wellman

{"title":"Persistent Erythrocytosis in a Dog With a Spinal Sclerosing Paraganglioma.","authors":"Samuel V Neal, Samantha L Sommer, Sarah Golconda, Eric T Hostnik, Russell Sakacs, Janice Park, Ashley Potts, Ronaldo C da Costa, Christopher Premanandan, Kara Corps, Sanggu Kim, Maxey L Wellman","doi":"10.1111/vcp.70112","DOIUrl":"https://doi.org/10.1111/vcp.70112","url":null,"abstract":"A 7-year-old, male, castrated, mixed-breed dog presented with right pelvic limb lameness and pain on posturing to defecate that began 1.5 years prior to presentation. Radiographs taken 8 months prior to presentation showed a small lytic lesion of the L6 vertebral body, and CBCs showed a persistently increased hematocrit (HCT) for at least 2 years. Lumbar magnetic resonance imaging (MRI) revealed a right-sided L5-L7 intradural-extramedullary mass. Cytologic evaluation of a fine-needle aspirate of the mass was consistent with a diagnosis of malignant neoplasia, with a primary differential of paraganglioma. Additional testing revealed an increased normetanephrine-to-creatinine ratio and low serum erythropoietin (EPO). Histologic evaluation, immunohistochemistry, and electron microscopy were consistent with a diagnosis of spinal sclerosing paraganglioma. No mutations were found on DNA sequencing that are associated with polycythemia vera in humans, a dog with primary erythrocytosis, or in humans with polycythemia-paraganglioma syndrome (PPS). The patient was treated with palliative radiation, after which the neurologic deficits markedly improved and the erythrocytosis resolved. The patient continued to do well with an HCT within the reference interval. Two years later, the patient's neurologic signs recurred, and MRI revealed the paraganglioma had progressed in size. The patient continued to have an HCT within the reference interval and underwent repeat palliative radiation with no complications. The concurrent erythrocytosis and paraganglioma in this patient resemble reports of PPS in humans.","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analytical Validation of an Automated Point-of-Care Immunoassay for the Measurement of Canine Pancreatic Lipase Immunoreactivity Concentration (Vcheck cPL 2.0). 用于测量犬胰脂肪酶免疫反应性浓度（Vcheck cpl2.0）的自动即时免疫分析法的分析验证。

IF 1.1 4区农林科学

Veterinary clinical pathology Pub Date : 2026-05-01 DOI: 10.1111/vcp.70106

Isabel Mendoza-White, Joerg M Steiner, Harry Cridge

{"title":"Analytical Validation of an Automated Point-of-Care Immunoassay for the Measurement of Canine Pancreatic Lipase Immunoreactivity Concentration (Vcheck cPL 2.0).","authors":"Isabel Mendoza-White, Joerg M Steiner, Harry Cridge","doi":"10.1111/vcp.70106","DOIUrl":"https://doi.org/10.1111/vcp.70106","url":null,"abstract":"Background: Pancreatic lipase assays are commonly utilized in the diagnostic approach to suspected pancreatitis in dogs. While a previously available point-of-care assay for the quantification of canine pancreatic lipase immunoreactivity (Vcheck cPL 1.0) performed sub-optimally, the updated version (Vcheck cPL 2.0) lacks independent validation.Objective: To evaluate the analytic validity of the Vcheck cPL 2.0 assay.Methods: Linearity was assessed via dilutional parallelism. Intra- and inter-assay variability were assessed by calculating coefficients of variation across replicates on the same day and across consecutive days, respectively. Interference studies were performed to assess the effects of bilirubin, hemoglobin, and lipid presence in test samples on assay performance.Results: Linearity appeared within target parameters at pancreatic lipase concentrations up to ~800 md/dL (mean O/E ratio: 0.968 ± 0.104) but was unacceptable at pancreatic lipase concentrations > 2000 mg/dL (mean O/E ratio: 1.432 ± 0.178). Intra-assay precision (median coefficient of variation 7.2%) was acceptable; however, inter-assay precision was sub-optimal (coefficient of variation > 10%) in 50% of samples analyzed. Variable precision may result in different diagnostic interpretations. Icterus and lipemia did not impact assay results. However, hemolysis, when severe (375-500 mg/dL), may impact test results (p < 0.005).Conclusions: The Vcheck cPL 2.0 shows improved precision compared to previous versions. Linearity was unacceptable at higher pancreatic lipase concentrations and hemolysis may impact Vcheck cPL 2.0 assay results.","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0