Roberta Perego, Eva Spada, Luciana Baggiani, Giuliano Ravasio, Enrica Zucca, Graziella Vanosi, Giancarlo Ruffo, Daniela Proverbio
{"title":"犬白细胞诱导的低温沉淀、低温血浆和新鲜血浆止血活性的比较。","authors":"Roberta Perego, Eva Spada, Luciana Baggiani, Giuliano Ravasio, Enrica Zucca, Graziella Vanosi, Giancarlo Ruffo, Daniela Proverbio","doi":"10.1111/vcp.70017","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>To date, no studies have reported the evaluation of hemostatic activity in canine leukoreduced cryoprecipitate (LR-CRYO) and leukoreduced cryopoor plasma (LR-CPP).</p>\n </section>\n \n <section>\n \n <h3> Objectives</h3>\n \n <p>We aimed to compare the hemostatic activity of LR-CRYO and LR-CPP to leukoreduced fresh plasma (LR-FP) and to evaluate the preservation of LR-CRYO by refrigeration and refreezing after thawing.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Four hundred fifty milliliters of fresh blood was collected from ten donor dogs, leukoreduced, and separated into LR-FP, then frozen (−20°C) to obtain leukoreduced fresh frozen plasma (LR-FFP). LR-FFP was further separated into LR-CRYO and LR-CPP. LR-CRYO was frozen, thawed, and divided into two bags, one refrigerated for 24 h and one refrozen for 7 days. Factor VIII (FVIII) and X (FX) activity, prothrombin time, activated partial thromboplastin time, antithrombin III (ATIII) activity (ATA), total protein, albumin, fibrinogen, and D-dimer concentration, and von Willebrand Factor (vWF) activity were measured in LR-FP, LR-CRYO, LR-CPP, refrigerated, and refrozen LR-CRYO.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>FVIII activity was higher in LR-CRYO (<i>p</i> = 0.0001) versus LR-FP. vWF activity (<i>p</i> < 0.0001) and fibrinogen concentration (<i>p</i> = 0.0012) were lower in LR-CPP versus LR-FP. FX activity was higher in LR-CPP (<i>p</i> < 0.0001) and LR-FP (<i>p</i> = 0.0002) versus LR-CRYO, and albumin concentration was higher in LR-CPP versus LR-FP (<i>p</i> < 0.0001) and LR-CRYO (<i>p</i> < 0.0001). No statistically significant difference was found in refrigerated or refrozen LR-CRYO as compared with LR-CRYO, excluding ATA, which was lower (<i>p</i> = 0.0062) in refrigerated LR-CRYO.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>Because the concentration of FVIII is higher in LR-CRYO than in LR-FP, LR-CRYO is a possible component therapy when this factor is deficient. Since no statistically significant difference was found in refrozen LR-CRYO as compared with LR-CRYO, LR-CRYO can be frozen after thawing for reuse.</p>\n </section>\n </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 2","pages":"120-131"},"PeriodicalIF":1.1000,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.70017","citationCount":"0","resultStr":"{\"title\":\"Comparison of Hemostatic Activity in Canine Leukoreduced Cryoprecipitate, Cryopoor Plasma, and Fresh Plasma\",\"authors\":\"Roberta Perego, Eva Spada, Luciana Baggiani, Giuliano Ravasio, Enrica Zucca, Graziella Vanosi, Giancarlo Ruffo, Daniela Proverbio\",\"doi\":\"10.1111/vcp.70017\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>To date, no studies have reported the evaluation of hemostatic activity in canine leukoreduced cryoprecipitate (LR-CRYO) and leukoreduced cryopoor plasma (LR-CPP).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Objectives</h3>\\n \\n <p>We aimed to compare the hemostatic activity of LR-CRYO and LR-CPP to leukoreduced fresh plasma (LR-FP) and to evaluate the preservation of LR-CRYO by refrigeration and refreezing after thawing.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>Four hundred fifty milliliters of fresh blood was collected from ten donor dogs, leukoreduced, and separated into LR-FP, then frozen (−20°C) to obtain leukoreduced fresh frozen plasma (LR-FFP). LR-FFP was further separated into LR-CRYO and LR-CPP. LR-CRYO was frozen, thawed, and divided into two bags, one refrigerated for 24 h and one refrozen for 7 days. Factor VIII (FVIII) and X (FX) activity, prothrombin time, activated partial thromboplastin time, antithrombin III (ATIII) activity (ATA), total protein, albumin, fibrinogen, and D-dimer concentration, and von Willebrand Factor (vWF) activity were measured in LR-FP, LR-CRYO, LR-CPP, refrigerated, and refrozen LR-CRYO.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>FVIII activity was higher in LR-CRYO (<i>p</i> = 0.0001) versus LR-FP. vWF activity (<i>p</i> < 0.0001) and fibrinogen concentration (<i>p</i> = 0.0012) were lower in LR-CPP versus LR-FP. FX activity was higher in LR-CPP (<i>p</i> < 0.0001) and LR-FP (<i>p</i> = 0.0002) versus LR-CRYO, and albumin concentration was higher in LR-CPP versus LR-FP (<i>p</i> < 0.0001) and LR-CRYO (<i>p</i> < 0.0001). No statistically significant difference was found in refrigerated or refrozen LR-CRYO as compared with LR-CRYO, excluding ATA, which was lower (<i>p</i> = 0.0062) in refrigerated LR-CRYO.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>Because the concentration of FVIII is higher in LR-CRYO than in LR-FP, LR-CRYO is a possible component therapy when this factor is deficient. Since no statistically significant difference was found in refrozen LR-CRYO as compared with LR-CRYO, LR-CRYO can be frozen after thawing for reuse.</p>\\n </section>\\n </div>\",\"PeriodicalId\":23593,\"journal\":{\"name\":\"Veterinary clinical pathology\",\"volume\":\"54 2\",\"pages\":\"120-131\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2025-06-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.70017\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary clinical pathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/vcp.70017\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary clinical pathology","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/vcp.70017","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Comparison of Hemostatic Activity in Canine Leukoreduced Cryoprecipitate, Cryopoor Plasma, and Fresh Plasma
Background
To date, no studies have reported the evaluation of hemostatic activity in canine leukoreduced cryoprecipitate (LR-CRYO) and leukoreduced cryopoor plasma (LR-CPP).
Objectives
We aimed to compare the hemostatic activity of LR-CRYO and LR-CPP to leukoreduced fresh plasma (LR-FP) and to evaluate the preservation of LR-CRYO by refrigeration and refreezing after thawing.
Methods
Four hundred fifty milliliters of fresh blood was collected from ten donor dogs, leukoreduced, and separated into LR-FP, then frozen (−20°C) to obtain leukoreduced fresh frozen plasma (LR-FFP). LR-FFP was further separated into LR-CRYO and LR-CPP. LR-CRYO was frozen, thawed, and divided into two bags, one refrigerated for 24 h and one refrozen for 7 days. Factor VIII (FVIII) and X (FX) activity, prothrombin time, activated partial thromboplastin time, antithrombin III (ATIII) activity (ATA), total protein, albumin, fibrinogen, and D-dimer concentration, and von Willebrand Factor (vWF) activity were measured in LR-FP, LR-CRYO, LR-CPP, refrigerated, and refrozen LR-CRYO.
Results
FVIII activity was higher in LR-CRYO (p = 0.0001) versus LR-FP. vWF activity (p < 0.0001) and fibrinogen concentration (p = 0.0012) were lower in LR-CPP versus LR-FP. FX activity was higher in LR-CPP (p < 0.0001) and LR-FP (p = 0.0002) versus LR-CRYO, and albumin concentration was higher in LR-CPP versus LR-FP (p < 0.0001) and LR-CRYO (p < 0.0001). No statistically significant difference was found in refrigerated or refrozen LR-CRYO as compared with LR-CRYO, excluding ATA, which was lower (p = 0.0062) in refrigerated LR-CRYO.
Conclusions
Because the concentration of FVIII is higher in LR-CRYO than in LR-FP, LR-CRYO is a possible component therapy when this factor is deficient. Since no statistically significant difference was found in refrozen LR-CRYO as compared with LR-CRYO, LR-CRYO can be frozen after thawing for reuse.
期刊介绍：
Veterinary Clinical Pathology is the official journal of the American Society for Veterinary Clinical Pathology (ASVCP) and the European Society of Veterinary Clinical Pathology (ESVCP). The journal''s mission is to provide an international forum for communication and discussion of scientific investigations and new developments that advance the art and science of laboratory diagnosis in animals. Veterinary Clinical Pathology welcomes original experimental research and clinical contributions involving domestic, laboratory, avian, and wildlife species in the areas of hematology, hemostasis, immunopathology, clinical chemistry, cytopathology, surgical pathology, toxicology, endocrinology, laboratory and analytical techniques, instrumentation, quality assurance, and clinical pathology education.
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