Veterinary clinical pathology最新文献

{"title":"Serum total antioxidant status in dogs: Reference intervals and influence of multiple biological and analytical factors","authors":"B. Perez-Montero,&nbsp;M. L. Fermin-Rodriguez,&nbsp;M. Portero-Fuentes,&nbsp;J. Sarquis,&nbsp;S. Caceres,&nbsp;J. C. Illera del Portal,&nbsp;L. de Juan,&nbsp;G. Miro,&nbsp;F. Cruz-Lopez","doi":"10.1111/vcp.13381","DOIUrl":"10.1111/vcp.13381","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Total antioxidant status (TAS) is one of the most widely used oxidative stress biomarkers, but the lack of canine RI and the influence of analytical factors hinder its application in clinical practice.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>The aims of this study were to establish canine assay-specific RI for TAS and evaluate the sources of biological variation and the association between TAS and multiple hematologic and biochemical variables.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Blood samples from 190 clinically healthy dogs were collected, encompassing pet dogs (82), police dogs (56), and shelter dogs (52). After hematologic and biochemical analysis, serum TAS was determined by means of a commercial 2,2′-azinobis (3-ethylbenzthiazolin-6-sulfonic acid) (ABTS) test. The American Society for Veterinary Clinical Pathology guidelines were followed to establish the RI, employing nonparametric methods. Univariate analysis and multivariate analysis were conducted to assess the influence of biological and analytical variables, yielding a final regression model.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The final reference population comprised 143 dogs, for which the RI was established (1.41–2.27 mmol/L). Partitioning was applied to the three study groups. The regression model revealed that police dogs had significantly higher TAS values than pet dogs. Furthermore, significant associations between four biochemical variables (albumin, globulins, cholesterol, and aspartate aminotransferase) and serum TAS were found.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>This is the first study to establish RI for serum TAS in a large and heterogeneous canine population and provide data on its relationship with analytical variables. These findings could potentially improve the interpretation of TAS in clinical environments.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"53 4","pages":"399-408"},"PeriodicalIF":1.2,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11735657/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142475937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multicentric aggressive unclassified hematopoietic neoplasm involving the placenta in a pregnant bitch","authors":"Laura Marconato,&nbsp;Silvia Sabattini,&nbsp;Daniele Zambelli,&nbsp;Maria Giulia Ferrari,&nbsp;Luca Aresu,&nbsp;Andrea Renzi,&nbsp;Anna Ferrari,&nbsp;Marco Cunto,&nbsp;Ilaria Maga,&nbsp;Giulia Ballotta","doi":"10.1111/vcp.13394","DOIUrl":"10.1111/vcp.13394","url":null,"abstract":"<p>Hematopoietic neoplasms are common in dogs; however, their association with pregnancy has not been previously reported in veterinary medicine. This rare occurrence presents a variety of diagnostic, therapeutic, prognostic, and ethical challenges. We report a case of a 3-year-old pregnant Bernese Mountain Dog diagnosed with multicentric aggressive unclassified hematopoietic cancer associated with paraneoplastic hypercalcemia during pregnancy. The dog died 7 days after diagnosis, and at Day 36 of pregnancy before any treatment decision could be made. Post-mortem evaluation, including histology, immunohistochemistry, and clonality analysis, led to the diagnosis of an unclassified hematopoietic cancer affecting the uterus and placenta, with no evidence of fetal involvement. The placenta likely acted as a barrier, preventing neoplastic involvement of the fetuses. Alternatively, the pregnancy might have been too early for the hematopoietic neoplasm to affect the labyrinth zone of the placenta and the fetuses. The dramatic disease progression could be explained by compromised cell-mediated immunity during pregnancy. This immunodeficient state is induced by embryonic, maternal, and hormonal factors, which suppress the response to mitogens to prevent rejection of the placenta and the conceptuses. Thus, pregnant dogs might exhibit increased vulnerability to cancer and infectious diseases that rely on cell-mediated immunity for host defense.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"53 4","pages":"442-447"},"PeriodicalIF":1.2,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142393689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements in genetic analysis: Insights from a case study and review of next-generation sequencing techniques for veterinary oncology applications.","authors":"R Adam Harris, Jillian Nolan, Dylan Ammons, Samantha Beeson, Douglas Thamm, Anne Avery","doi":"10.1111/vcp.13388","DOIUrl":"https://doi.org/10.1111/vcp.13388","url":null,"abstract":"<p><p>Acute myeloid leukemia (AML) poses significant challenges in veterinary medicine, with limited treatment options and poor survival rates. While substantial progress has been made in characterizing human AML, translating these advancements to veterinary practice has been hindered by limited molecular understanding and diagnostic tools. The case study presented illustrates the application of whole genome sequencing in diagnosing AML in a dog, showcasing its potential in veterinary oncology. Our approach facilitated comprehensive genomic analysis, identifying mutations in genes that may be associated with AML pathogenesis in dogs, such as KRAS, IKZF1, and RUNX1. However, without supportive evidence of its clinical utility (eg, association with response to treatment or prognosis), the information is limited to exploration. This article reviews the comparative features of canine AML with human AML and discusses strategies to shrink the knowledge gap between human and veterinary medicine with cost-effective next-generation sequencing (NGS) techniques. By utilizing these approaches, the unique and shared molecular features with human AML can be identified, aiding in molecular classification and therapeutic development for both species. Despite the promise of NGS, challenges exist in implementing it into routine veterinary diagnostics. Cost considerations, turnaround times, and the need for robust bioinformatics pipelines and quality control measures must be addressed. Most importantly, analytical and clinical validation processes are essential to ensure the reliability and clinical utility of NGS-based assays. Overall, integrating NGS technologies into veterinary oncology holds great potential for advancing our understanding of AML and improving disease stratification, in hopes of improving clinical outcomes.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142376112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What is your diagnosis? Impression smears of an intracranial mass from a dog","authors":"Michael A. Talavera,&nbsp;Kayla J. Alexander,&nbsp;Andy Shores,&nbsp;Marjorie J. Williams,&nbsp;Matthew L. Williams","doi":"10.1111/vcp.13391","DOIUrl":"10.1111/vcp.13391","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 S1","pages":"S16-S18"},"PeriodicalIF":1.1,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What is your diagnosis? Fine-needle aspirate from a neck lesion in a dog","authors":"Cynthia Robveille,&nbsp;Marie Cuvelier","doi":"10.1111/vcp.13389","DOIUrl":"10.1111/vcp.13389","url":null,"abstract":"&lt;p&gt;An 8-year-old spayed female Labrador dog presented a bilateral, slightly asymmetric mass measuring approximately 2.5 cm in diameter in the neck. The owner noticed the lesion 10 days previously; it was sore during the physical examination. Ultrasound or computed tomography of the neck was not performed. As sialoadenitis was suspected, the dog received antibiotics for 1 week, but no clinical improvement was noticed. The diagnostic workup included a complete blood count, level of C-reactive protein, and imaging (thoracic radiography, abdominal ultrasound); no abnormalities were revealed. A fine-needle aspirate of the lesion was submitted for cytologic examination (Figure 1).&lt;/p&gt;&lt;p&gt;&lt;b&gt;Cytologic interpretation:&lt;/b&gt; Malignant neoplasm with epithelioid appearance.&lt;/p&gt;&lt;p&gt;The aspirate of the cervical mass was similar on both sides, with moderate-to-high cellularity. The exact location of the mass was not determined. A moderate number of RBCs were present. The predominant population was atypical cells distributed in dense and loose cohesive clusters and less frequently in single cells (Figure 2). Several pseudoacinar structures were present. A small amount of pink extracellular matrix was rarely observed (Figure 3A). Cells were polygonal to infrequently spindle, with variably distinct cell borders and a moderate amount of pale basophilic cytoplasm. Small clear vacuoles were sometimes present in the cytoplasm (Figure 3). Cells with a single large cytoplasmic vacuole (signet ring-like cells) or erythrophagocytosis were rare. Nuclei measured two times the size of an erythrocyte and were oval, eccentric, or less frequently central, with finely stippled chromatin and one to four variably prominent nucleoli. Atypia was moderate: anisocytosis, anisokaryosis, and rare binucleation. Mitotic figures were not observed. The cells were admixed with occasional mast cells, plasma cells, hemosiderin-laden macrophages, and scattered neutrophils.&lt;/p&gt;&lt;p&gt;Excisional biopsy was performed 15 days after the first consultation. The sample, identified as a submandibular lymph node by the veterinary surgeon, was a pear-shaped nodule (2.1 cm in length; 1.5–1 cm in diameter) and was diffusely dark red on cross section. Histologic evaluation revealed an unencapsulated, infiltrative, densely cellular neoplasm, replacing almost all the preexisting lymph node. The latter was identified because of the presence of subcapsular sinuses, trabeculae lined by a well-differentiated endothelium, and rare lymphoid follicles. The sample also included a non-neoplastic lymph node adjacent to the neoplastic lesion, showing sinus erythrocytosis. Neoplastic cells were spindle to polygonal cells forming blood-filled vascular channels supported by a thin collagenous core and, to a lesser extent, solid areas (Figure 4). They had indistinct cell borders, small-to-moderate amount of eosinophilic cytoplasm, and oval nuclei with finely stippled chromatin and frequently a single prominent nucleolus. The atypi","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 S1","pages":"S12-S15"},"PeriodicalIF":1.1,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.13389","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What is your diagnosis? Cerebrospinal fluid from an Angus cow","authors":"Megan G. Palmisano,&nbsp;Koranda Walsh,&nbsp;Susan Bender,&nbsp;Elizabeth Nelson,&nbsp;Rose Nolen-Walston","doi":"10.1111/vcp.13383","DOIUrl":"10.1111/vcp.13383","url":null,"abstract":"&lt;p&gt;A 6-year-old Angus cow was presented on emergency to a tertiary referral hospital with a 2-week history of progressive recumbency. The cow had calved 1 month prior to presentation with notable paresis 2 weeks postpartum. The cow showed no improvement despite treatment with flunixin and dexamethasone at the farm.&lt;/p&gt;&lt;p&gt;Pertinent examination findings included lateral recumbency with an ability to sit sternal, tachypnea (52 breaths/min, RI 20–32), and peripheral lymphadenopathy. The patient's mentation was normal, with no cranial nerve deficits and no muscle atrophy. Both orthopedic evaluation and abdominal palpation per rectum were unremarkable. Serum biochemistry, CBC, including blood smear evaluation, and beta-hydroxybutyrate (BHB) ketones were within the reference intervals. Fibrinogen concentration was increased (1044 mg/dL, RI 300–775), and the California mastitis test (CMT) was negative.&lt;/p&gt;&lt;p&gt;A neurologic etiology was suspected based on the inability rise and a thoracolumbar/lumbosacral neurolocalization was made. Lumbosacral cerebrospinal fluid (CSF) centesis was performed as a rule-out for neurologic etiologies for recumbency. CSF was submitted for cytologic evaluation (Figure 1). The CSF was clear with a total protein of 166 mg/dL (RI &lt; 40 mg/dL), RBC count of 3250 cells/μL (RI 0 cells/μL), and nucleated cell count of 18 cells/μL (RI 0–3 cells/μL).&lt;/p&gt;&lt;p&gt;The patient was given a working diagnosis of enzootic bovine leukosis (EBL) and lymphoma based on CSF analysis and positive serum BLV ELISA (Cornell University Animal Health Diagnostic Center), which reports both high sensitivity and specificity.&lt;span&gt;&lt;sup&gt;1&lt;/sup&gt;&lt;/span&gt; The owner elected euthanasia, given the persistent paresis despite flotation therapy and dexamethasone treatment. Postmortem histopathologic examination of multiple lymph nodes (retroperitoneal and cervical) was performed to support the antemortem diagnosis. The lymph node aspirate was critical to supporting the diagnostic findings of CSF analysis but was not performed as the initial diagnostic of choice due to concern that the result would not definitively diagnose EBL as the cause of the cow's paresis. The lymph nodes were multifocally to diffusely expanded by sheets of neoplastic round cells that distorted the nodal architecture. The cells had scant eosinophilic cytoplasm and large round nuclei approximately two to three times the diameter of an erythrocyte, with coarsely stippled chromatin and one to two prominent nucleoli. Nuclear pleomorphism was moderate, and mitotic figures numbered up to 22 per single high-power (2.37 mm&lt;sup&gt;2&lt;/sup&gt;) field (Figure 2A,B).&lt;/p&gt;&lt;p&gt;Two clinical entities of lymphoma documented in cattle are sporadic and enzootic bovine leukosis, the latter being more common and found in association with bovine leukemia virus (BLV) infection.&lt;span&gt;&lt;sup&gt;2, 3&lt;/sup&gt;&lt;/span&gt; BLV, a retrovirus, is known to be the causative agent of EBL and is a lymphoproliferative infection spread in secretions from inf","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 S1","pages":"S51-S53"},"PeriodicalIF":1.1,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.13383","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Canine and feline liver cytology ( 1st ed.) by Carlo Masserdotti (Ed.), Hoboken, NJ: Wiley-Blackwell. 2023. pp. 288. ISBN: 978-1-119-89555-8—US$149.99 (Hardcover)","authors":"Francisco O. Conrado","doi":"10.1111/vcp.13384","DOIUrl":"https://doi.org/10.1111/vcp.13384","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"53 3","pages":"374-375"},"PeriodicalIF":1.2,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142316696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Death and taxes: Lactate and the Laffer curve","authors":"Rose D. Nolen-Walston,&nbsp;Michael J. Mandel","doi":"10.1111/vcp.13387","DOIUrl":"10.1111/vcp.13387","url":null,"abstract":"<p>In 1980, Ronald Reagan was elected as a president on a simple platform: the need for tax cuts to revive the economy, which was struggling at the time. His theory, and the theory of the supply-side economists who supported him, was simple. Business owners are more likely to hire new workers and invest in new equipment if their after-tax income goes up. The supply-siders of the day believed that if federal tax rates were cut, especially at the top end, business owners would expand, boosting both employment and investment, increasing growth, and potentially even reducing inflation, which was a big problem at the time. Thus, the benefits of the tax cut would “trickle down” to all Americans. One of the main proponents of this theory was an economist called Arthur Laffer, who, in 1974, had been enjoying a late-night supper with a few Republican policy wonks (including Dick Cheney and Donald Rumsfeld, as it happened). The topic of conversation was tax rate and government income and whether President Ford's tax hikes would spur increases in federal revenue. Not necessarily, suggested Laffer, who reportedly whipped out a pen and made the following sketch on his napkin (Figure 1).<span>¹</span>\u0000 </p><p>On the x-axis, we see tax rates ranging from 0% to 100%, and on the y-axis, there is government revenue. It is clear that a tax rate of 0% results in zero income, and the coffers are dry. Conversely, though, Laffer pointed out that a tax rate of 100% would result in the same thing. If the government takes every cent of your salary, you are clearly not going to work at all. So, what if, suggests Laffer's curve, we are here, point A in Figure 2, on the tax curve?</p><p>In this case, high tax rates are actually suppressing taxpayer's capitalist urges. A reduction in the tax rate to point B will paradoxically result in an increase in revenue, not a decrease, as shown here in Figure 3.</p><p>This was an odd thought. Could a drop in the tax rate really increase the amount of money the government collected? We avoid political commentary and allow readers their own interpretation of history as to the results of this experiment.<span>²</span> However, this concept has an interesting application in the consideration of physiology.</p><p>Take lactate, for example. L-lactate is produced by the mammalian cell as a product of anaerobic metabolism,<span>³</span> and is often used clinically as a measure of tissue perfusion. Blood lactate concentration not only assesses both the rate of production as well as clearance,<span>⁴</span> but also, in the very end, it is limited by the capacity of the heart and peripheral vasculature to mobilize the tissue-produced lactate and carry venous blood back to the sampling point. As such, lactate acts as the scorecard of the body; though blood lactate levels cannot tell you what is wrong or how to fix it, it provides useful information to clinicians as to whether they are winning","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"53 3","pages":"291-293"},"PeriodicalIF":1.2,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.13387","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142308623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acid–base and electrolyte changes in dogs after packed red blood cell transfusion","authors":"P. Bou,&nbsp;I. Mesa,&nbsp;R. F. Ferreira,&nbsp;C. Torrente,&nbsp;E. G. Manzanilla,&nbsp;L. Bosch","doi":"10.1111/vcp.13379","DOIUrl":"10.1111/vcp.13379","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Packed RBC (pRBC) transfusions are often necessary to enhance organ perfusion and tissue oxygenation in cases of severe anemia.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>We aimed to describe changes in acid–base and biochemical parameters in dogs after transfusion of pRBC and potential effects on the outcome.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The prospective observational study included anemic dogs requiring pRBC transfusions. Venous blood gas and electrolytes were measured pre-transfusion and repeated within 1 h post-transfusion. Signalment, pre-existing conditions and underlying disease(s), number of days spent of hospitalization, the quantity of transfused pRBC units, and the outcome were also recorded. Associations between analytical values and all other parameters before and after transfusion were assessed using the Wilcoxon test. A generalized linear model was generated for each of the blood gas parameters post-transfusion, including the pre-transfusion values and the characteristics of the animal as fixed effects. The effect of the blood gas parameters on the mortality and hospitalization length was assessed using logistic regression and generalized linear models, respectively.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Twenty-six dogs with different causes of anemia were included in the study, with dogs remaining hospitalized for a median of 3 days (range: 0–11). Pre-transfusion heart rate, respiratory rate, Angap, base excess, and lactate were higher than post-transfusion values. Post-transfusion results showed an increase in hematocrit, hemoglobin, HCO₃-, PCO₂, tCO₂, and chloride. Pre-transfusion hypophosphatemia and receiving an increased volume of transfused pRBC were associated with a longer hospital stay, while higher pre-transfusion lactate values were associated with higher mortality.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>pRBC transfusion in anemic dogs was associated with an improvement in perfusion and acid–base parameters. Pre-transfusion lactate may be an indicator of mortality in dogs.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"53 4","pages":"392-398"},"PeriodicalIF":1.2,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11735664/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thromboelastography in dogs with idiopathic epilepsy treated with phenobarbital monotherapy","authors":"R. García,&nbsp;J. Pastor,&nbsp;C. de la Fuente,&nbsp;S. Añor","doi":"10.1111/vcp.13380","DOIUrl":"10.1111/vcp.13380","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Thromboelastography (TEG) is an effective technique to assess the efficiency of coagulation. Phenobarbital (PB) can induce hematological and coagulation disorders in both animals and humans, but its effects on hemostasis have been little investigated and are poorly understood in dogs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>The aim of this article was to assess coagulation using TEG in a population of dogs with idiopathic epilepsy treated with PB.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>Prospective observational study. TEG was performed in blood samples from dogs with idiopathic epilepsy that were divided in three groups: Two groups of treated dogs that were on phenobarbital treatment for less or more than 6 months, and a control group of healthy dogs. Duration of treatment, dose, phenobarbital serum concentration and selected hematological and biochemical parameters were evaluated and correlated with the TEG results.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>No statistically significant differences were found between groups. None of the animals appeared to be in a hypo- or hypercoagulable state, however 9/19 (47,4%) dogs were classified as hyper-fibrinolytic. A statistically significant negative relationship between MA and G values and increased fibrinolytic activity (LY30) were found. No statistically significant relationship was found between PB dose or PB blood levels and TEG parameters in either group. No bleeding complications were observed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Discussion</h3>\u0000 \u0000 <p>The rise in fibrinolysis might be due to hepatic damage from PB, as indicated by elevated liver enzymes in many dogs with abnormal fibrinolytic patterns. Although TEG showed hyperfibrinolysis in some dogs, the presence of primary or secondary hyperfibrinolysis could not be confirmed due to the lack of D-dimer measurements and liver biopsy. TEG’s sensitivity compared to other fibrinolysis biomarkers like PAP might also affect results.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>TThe cause of hyperfibrinolysis in epileptic dogs treated with phenobarbital remains unclear, with potential links to hepatic effects or handling, and further research is needed to assess its clinical significance.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"53 4","pages":"385-391"},"PeriodicalIF":1.2,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11735646/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}