冷球蛋白血症并发利什曼婴儿感染的狗和它的干扰与两个自动血液学分析仪。

IF 1.2 4区 农林科学 Q3 VETERINARY SCIENCES
Javier Martínez-Caro, Beatriz Agulla, Alba Benet, Josep Pastor
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引用次数: 0

摘要

一只6岁,完整的雄性英国斗牛犬被提出评估弱点。体格检查发现轻度黏膜苍白和眼部病变。在Urit Smart V5和Sysmex XN-1000V上进行了全血细胞计数,结果显示两种分析仪之间存在一些血液学差异。Urit报告了明显的白细胞增多和血小板增多,Sysmex报告了阻抗和光学RET通道上明显的血小板增多,但光学PLT-F通道上轻度的血小板减少。在血液涂片检查中,有丰富的与冷球蛋白相容的蛋白质物质。红细胞表现出明显程度的异数红细胞增多。白细胞数量与Sysmex总白细胞计数相关,而血小板计数与Sysmex光学PLT-F通道的相关性更好。将血液在毛细管中离心(室温下),鉴定出一种冷球蛋白沉淀物。此外,将血液加热到37°C后很快重新分析,这部分地纠正了大多数干扰。血清蛋白电泳显示明显的高蛋白血症伴轻度低白蛋白血症和明显的高丙种球蛋白血症。诊断影像显示轻度脾肿大。脾细胞学检查显示婴儿利什曼原虫感染,浆细胞增生,髓外造血。这是对感染利什曼原虫的狗的首次描述。并发单克隆高γ球蛋白血症和冷球蛋白血症的婴儿。我们详细描述了低温球蛋白与Urit Smart V5和Sysmex XN-1000V的干扰,以及Sysmex PLT-F在这种情况下的用途。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cryoglobulinemia concurrent with Leishmania infantum infection in a dog and its interference with two automated hematology analyzers.

A 6-year-old, intact male English Bulldog presented for evaluation of weakness. Physical examination revealed mildly pale mucous membranes and ophthalmologic alterations. A complete blood cell count was performed on the Urit Smart V5 and the Sysmex XN-1000V, and showed several hematologic discrepancies between the analyzers. The Urit reported marked leukocytosis and thrombocytosis, and the Sysmex reported marked thrombocytosis on the impedance and optical RET channel but mild thrombocytopenia on the optical PLT-F channel. On the blood smear examination, there was an abundant proteinaceous material compatible with cryoglobulins. The red blood cell exhibited a notable degree of poikilocytosis. The number of leukocytes was found to be correlated with the Sysmex total white blood cell count, while the platelet count demonstrated a better correlation with the optical PLT-F channel from the Sysmex. Centrifugation of the blood in a capillary tube (at room temperature) identified a cryoglobulin precipitate. Additionally, the blood was reanalyzed soon after warming it to 37°C, which partially corrected most of the interferences. The serum protein electrophoresis demonstrated a marked hyperproteinemia with mild hypoalbuminemia and a marked hypergammaglobulinemia. On diagnostic imaging, a mild splenomegaly was noted. Cytology of the spleen showed Leishmania infantum infection, plasma cell hyperplasia, and extramedullary hematopoiesis. This is the first description of a dog infected with Leishmania. infantum with concurrent monoclonal hypergammaglobulinemia and cryoglobulinemia. We present a detailed description of the interference of cryoglobulin with the Urit Smart V5 and the Sysmex XN-1000V, along with the usefulness of Sysmex PLT-F in this condition.

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来源期刊
Veterinary clinical pathology
Veterinary clinical pathology 农林科学-兽医学
CiteScore
1.70
自引率
16.70%
发文量
133
审稿时长
18-36 weeks
期刊介绍: Veterinary Clinical Pathology is the official journal of the American Society for Veterinary Clinical Pathology (ASVCP) and the European Society of Veterinary Clinical Pathology (ESVCP). The journal''s mission is to provide an international forum for communication and discussion of scientific investigations and new developments that advance the art and science of laboratory diagnosis in animals. Veterinary Clinical Pathology welcomes original experimental research and clinical contributions involving domestic, laboratory, avian, and wildlife species in the areas of hematology, hemostasis, immunopathology, clinical chemistry, cytopathology, surgical pathology, toxicology, endocrinology, laboratory and analytical techniques, instrumentation, quality assurance, and clinical pathology education.
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