Translational pediatrics最新文献

{"title":"Balloon dilation therapy for managing anastomotic strictures subsequent to surgical correction of esophageal atresia.","authors":"Yan-Yu Gong, Wen-Jie Wu, Zhen Qin","doi":"10.21037/tp-2024-572","DOIUrl":"10.21037/tp-2024-572","url":null,"abstract":"<p><strong>Background: </strong>Anastomotic strictures (AS) following surgical repair of esophageal atresia (EA) are the most common cause of pediatric esophageal strictures. In this retrospective cohort study, we evaluated the safety and efficacy of balloon dilation in the treatment of AS following EA repair.</p><p><strong>Methods: </strong>Balloon dilation was performed on 31 children with AS following EA repair (the diameter of the balloon ranged from 6-15 mm). Esophagography was conducted before and after each dilation. Change from liquid diets to solid food, alleviation of vomiting, and significant weight gain during the 3 to 6 months of follow-up after balloon dilation constituted an effective treatment. The Chi-squared test was used for data analysis in this retrospective cohort study.</p><p><strong>Results: </strong>A total of 86 balloon dilations were performed on 31 children, with 78 being successful, resulting in a success rate of 90.7%. In addition, 13 children underwent balloon dilations assisted by digital subtraction angiography (DSA). Four children developed restenosis, and one child suffered from esophageal perforation after the initial dilation. At the 3- to 6-month follow-up, the treatment was efficacious in 26 children, with an overall effectiveness rate of 84%.</p><p><strong>Conclusions: </strong>Balloon dilation is a safe and efficacious treatment option for the treatment of AS following surgical repair of EA.</p>","PeriodicalId":23294,"journal":{"name":"Translational pediatrics","volume":"14 6","pages":"1256-1262"},"PeriodicalIF":1.5,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12268777/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chenodeoxycholic acid activates the TGR5/TRPA1-5-HT pathway to regulate intestinal motility in breastfed infants and mouse models.","authors":"Zihong Shan, Chenghui He, Qingyao Yang, Jingjing Xiong, Meng Li, Mingyao Meng, Yongkun Huang","doi":"10.21037/tp-2025-100","DOIUrl":"10.21037/tp-2025-100","url":null,"abstract":"<p><strong>Background: </strong>Previous research has indicated that chenodeoxycholic acid (CDCA) plays a significant role in intestinal motility and is implicated in some gastrointestinal (GI) disorders. However, the impact of CDCA on defecation patterns in infants, particularly those who are breastfed, remains unclear. This study explored the relationship between CDCA and defecation frequency in breastfed infants, focusing on the underlying mechanisms.</p><p><strong>Methods: </strong>We measured CDCA levels in stool samples of infants with varying defecation frequencies using metabolomics, along with studies to explore the impact of CDCA on murine colonic transit and with <i>in vitro</i> cellular studies to elucidate the underlying physiological mechanisms.</p><p><strong>Results: </strong>In our murine model, CDCA treatment increased GI transit distance, enhanced fecal water content, and reduced transit time, without causing significant colonic damage. Additionally, CDCA treatment enhanced 5-hydroxytryptamine (5-HT) secretion and upregulated signaling molecules such as tryptophan hydroxylase 1 (Tph1), G protein-coupled bile acid receptor 1 (TGR5), and transient receptor potential ankyrin 1 (TRPA1). Selective inhibition of these pathways suppressed 5-HT secretion and normalized intestinal motility parameters, confirming their mechanistic role. Moreover, CDCA treatment of rat insulinoma-derived RIN-14B cells also resulted in increased 5-HT secretion.</p><p><strong>Conclusions: </strong>These findings reveal that CDCA significantly correlates with defecation frequency in breastfed infants and likely influences intestinal motility through the TGR5/TRPA1-5-HT signaling axis.</p>","PeriodicalId":23294,"journal":{"name":"Translational pediatrics","volume":"14 6","pages":"1213-1229"},"PeriodicalIF":1.5,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12268862/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of different subtalar joint arthroereisis implants on pediatric flexible flatfoot: a finite element analysis.","authors":"Yun Jin, Wenhao Zheng, Chunhui Chen, Leyi Cai, Huanguang Xie","doi":"10.21037/tp-2025-352","DOIUrl":"10.21037/tp-2025-352","url":null,"abstract":"<p><strong>Background: </strong>Flexible flat foot is a relatively common disease treated by foot and ankle surgery, and subtalar arthroereisis (STA) has gradually become favored by foot and ankle surgeons due to its advantages. However, which sinus tarsi implant is optimal remains controversial. The purpose of this study was to compare the biomechanical outcomes of two types sinus tarsi implants of different sizes via finite element analysis.</p><p><strong>Methods: </strong>A finite element model of pediatric flexible flatfoot deformity was established in which virtual surgery of STA was simulated. Boundary and loading conditions were assumed from the data of previous finite element analysis study. The corrective ability in talar-first metatarsal angle, talonavicular coverage angle, calcaneus pitch angle, and arch height were compared and analyzed. The indexes of plantar stress distribution, the peak von Mises of medial and lateral columns, and strain of the medial ligaments were also compared and analyzed.</p><p><strong>Results: </strong>All the indexes were changed after the placement of the STA implants. The plantar pressure and the load of medial/lateral columns were redistributed, while the strain of the ligaments was also decreased to differing degrees. When the implants of HyProCure and Talar-Fit inserted, the parameters involved were significantly changed. Moreover, the parameters involved were changed obviously in the larger sizes of implant.</p><p><strong>Conclusions: </strong>Both types of implants are beneficial in the treatment of pediatric flexible flatfoot deformity; however, HyProCure exhibited a stronger effect than did Talar-Fit. For both HyProCure and Talar-Fit, the larger sizes were better than the smaller ones.</p>","PeriodicalId":23294,"journal":{"name":"Translational pediatrics","volume":"14 6","pages":"1296-1305"},"PeriodicalIF":1.5,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12268589/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A nomogram model based on routine serum markers for predicting the occurrence of primary cholangitis after Kasai operation for biliary atresia.","authors":"Chunxiao Yang, Tengfei Li, Pu Yu, Jianghua Zhan","doi":"10.21037/tp-2025-170","DOIUrl":"10.21037/tp-2025-170","url":null,"abstract":"<p><strong>Background: </strong>The Kasai procedure is still considered the optimal therapeutic approach for biliary atresia (BA). Nevertheless, the onset of postoperative cholangitis can impede the resolution of jaundice and significantly affect the overall prognosis of the disease. This study aims to develop a nomogram model that precisely forecasted the incidence of cholangitis after the Kasai procedure.</p><p><strong>Methods: </strong>This study retrospectively collected clinical, preoperative, and postoperative serological data from patients with BA who underwent the Kasai procedure at Tianjin Children's Hospital between January 2017 and November 2023. Utilizing multivariable analysis and logistic regression, a clinical nomogram model was developed to predict the occurrence of primary cholangitis postoperatively. To validate the model's accuracy, data from patients with BA at Xi'an Children's Hospital from January 2018 to November 2019 were employed.</p><p><strong>Results: </strong>We identified two independent predictors, neutrophil ratio post-operative to pre-operative ratio (NEU% PPR) and alkaline phosphatase post-operative to pre-operative ratio (ALP PPR), that were significantly associated with the occurrence of primary cholangitis following the Kasai procedure. These predictors were subsequently utilized to construct a nomogram model. The model exhibited an area under the curve (AUC) value of 0.829, surpassing the predictive capabilities of individual predictors. Additionally, through Kaplan-Meier (KM) analysis, we observed a significant correlation between ALP PPR and the occurrence of postoperative primary cholangitis, further supporting the reliability of our nomogram model.</p><p><strong>Conclusions: </strong>This study has successfully established a clinical prediction model that can effectively predict the occurrence of primary cholangitis following the Kasai procedure for BA.</p>","PeriodicalId":23294,"journal":{"name":"Translational pediatrics","volume":"14 6","pages":"1103-1116"},"PeriodicalIF":1.5,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12268703/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IgG4 elevation dilemma at the crossroads of helminth infection and IgG4-related disease: insights from a case report of pediatric paragonimiasis with persistent pericardial effusion.","authors":"Zhan Zhang, Hua Zhou, Feng Fang, Guo Ai","doi":"10.21037/tp-2024-531","DOIUrl":"10.21037/tp-2024-531","url":null,"abstract":"<p><strong>Background: </strong>Immunoglobulin G4 (IgG4)-related disease (IgG4-RD) is a systemic fibroinflammatory disorder defined by elevated serum IgG4, tissue IgG4+ plasma cell infiltration, and multi-organ involvement. Paradoxically, helminth infections may also trigger IgG4 elevation through Th2-polarized immune responses, creating diagnostic ambiguity when overlapping features occur. Critically, no studies have addressed whether IgG4-RD with helminthiasis can coexist in children or how to differentiate them when histopathology is unavailable.</p><p><strong>Case description: </strong>A 10-year-old boy with confirmed pulmonary paragonimiasis developed refractory pericardial effusion and markedly elevated IgG4 [11 g/L, 8.1× upper limit of normal (ULN)]. Despite five courses of praziquantel (75 mg/kg/day), effusions persisted for 8 weeks. Subsequent glucocorticoids (methylprednisolone 2 mg/kg/day) achieved rapid clinical resolution, though lacked histopathology IgG4-RD hallmarks. Longitudinal monitoring revealed a dynamic IgG4 decline (from 11 to 5.25 g/L) post-methylprednisolone therapy. However, residual IgG4 elevation (5.25 g/L, >3× ULN) and absent histopathological features of IgG4-RD left the diagnosis unresolved. Therapeutic monitoring revealed normalized eosinophils and imaging improvement, yet persistent IgG4 suggested potential immune dysregulation beyond parasitic clearance.</p><p><strong>Conclusions: </strong>This case highlights a critical diagnostic dilemma: IgG4 elevation in parasitic infections may mimic or coexist with IgG4-RD. The rapid glucocorticoid response argues against simple parasitic infections, yet incomplete IgG4 normalization post-treatment leaves coexistence unresolved. Clinicians must weigh these possibilities when anti-helminthic therapy fails, particularly when histopathology is absent. Prospective studies should define pediatric-specific IgG4 thresholds to disentangle infection from autoimmunity.</p>","PeriodicalId":23294,"journal":{"name":"Translational pediatrics","volume":"14 6","pages":"1362-1369"},"PeriodicalIF":1.5,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12268758/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intractable epilepsy in m.1630A>G carriers should be treated with a ketogenic diet on a trial basis.","authors":"Josef Finsterer","doi":"10.21037/tp-2025-194","DOIUrl":"10.21037/tp-2025-194","url":null,"abstract":"","PeriodicalId":23294,"journal":{"name":"Translational pediatrics","volume":"14 6","pages":"1383-1384"},"PeriodicalIF":1.5,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12268684/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The association and diagnostic value of maternal serum placental biomarkers for fetal hypospadias and cryptorchidism.","authors":"Chen Jiang, Huimin Zhang, Wen Zhang, Yiming Chen","doi":"10.21037/tp-2025-83","DOIUrl":"10.21037/tp-2025-83","url":null,"abstract":"<p><strong>Background: </strong>Clinically, the diagnosis of hypospadias and cryptorchidism presents a certain degree of complexity due to the variability in anatomical presentation and potential comorbid conditions. This study aimed to evaluate the diagnostic value of maternal serum placental biomarkers [first-trimester (FT) gestation: 10-13⁺⁶ weeks; second-trimester (ST) gestation: 15-20⁺⁶ weeks] to predict hypospadias and cryptorchidism.</p><p><strong>Methods: </strong>We performed a retrospective case-control study, and participants were divided into three groups: a hypospadias group (222 cases), a cryptorchidism group (120 cases), and a control group (311 cases). Receiver operating characteristic (ROC) curve, area under the curve (AUC), sensitivity, and specificity analyses were used to evaluate the performance of the different predictive models constructed with maternal serum pregnancy-associated plasma protein-A (PAPP-A), alpha-fetoprotein (AFP), unconjugated estriol (uE3), and ST free beta human chorionic gonadotropin (ST-free β-hCG) individually or in combination for hypospadias and cryptorchidism.</p><p><strong>Results: </strong>Maternal serum PAPP-A and uE3 multiple of median (MoM) levels in the hypospadias group were lower than these in the control group [0.70 (0.10-2.41) <i>vs.</i> 1.03 (0.33-2.69) MoM; 0.80 (0.25-1.71) <i>vs.</i> 1.01 (0.63-1.56) MoM], while AFP and ST-free β-hCG MoM levels in the hypospadias group were higher than these in the control group [1.40 (0.34-8.27) <i>vs.</i> 1.02 (0.33-3.29) MoM; 1.25 (0.66-3.56) <i>vs.</i> 1.02 (0.52-1.90) MoM]. Meanwhile, maternal serum PAPP-A, AFP, and uE3 MoM levels in the cryptorchidism group were all lower than these in the control group [0.92 (0.31-3.01) <i>vs.</i> 1.03 (0.33-2.69) MoM; 1.00 (0.57-1.98) <i>vs.</i> 1.02 (0.52-1.90) MoM; 0.94 (0.69-1.49) <i>vs.</i> 1.01 (0.63-1.56) MoM], but ST-free β-hCG MoM level in the cryptorchidism group was higher than that in the control group [1.04 (0.28-3.51) <i>vs.</i> 1.02 (0.33-3.29) MoM] (all P<0.001). The best models in the screening protocol were the combined screening of multiple markers (PAPP-A + AFP + uE3) to predict hypospadias (AUC =0.795, P<0.001, sensitivity =0.800, specificity =0.788), and the screening model (PAPP-A + uE3) to predict cryptorchidism (AUC =0.658, P=0.03, sensitivity =0.778, specificity =0.614). Overall accuracy of predictive models was not sufficient.</p><p><strong>Conclusions: </strong>The combined risk model of maternal serum PAPP-A, AFP and uE3 had a relatively better diagnostic value for hypospadias and cryptorchidism than the models constructed with other placental biomarkers individually or in combination.</p>","PeriodicalId":23294,"journal":{"name":"Translational pediatrics","volume":"14 6","pages":"1188-1200"},"PeriodicalIF":1.5,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12268510/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of robot-assisted and laparoscopic pyeloplasty with flexible ureteroscopy for managing concomitant ureteropelvic junction obstruction and renal calculi in children.","authors":"Hongshuai Jia, Guang Yue, Yuandong Tao, Pin Li, Yangyang Wu, Hualin Cao, Xiaowei Zhang, Lifei Ma, Tian Tao, Huixia Zhou","doi":"10.21037/tp-2024-557","DOIUrl":"10.21037/tp-2024-557","url":null,"abstract":"<p><strong>Background: </strong>Ureteropelvic junction obstruction (UPJO) with renal calculi is a common cause of renal impairment in pediatric patients. Surgical intervention, including laparoscopic pyeloplasty (LP) and robot-assisted laparoscopic pyeloplasty (RALP) combined with flexible ureteroscopy, are frequently employed to address both the obstruction and stones. This study aims to compare the safety and efficacy of RALP and LP combined with flexible ureteroscopy in treating pediatric UPJO with renal calculi.</p><p><strong>Methods: </strong>We conducted a retrospective study of pediatric patients with UPJO and renal calculi treated between March 2018 and June 2023. All patients underwent either RALP or conventional LP combined with flexible ureteroscopy. Preoperative imaging and standardized postoperative follow-up were used to assess surgical outcomes.</p><p><strong>Results: </strong>A total of 25 patients were included, with 11 undergoing RALP and 14 undergoing LP. Stone clearance rates were 90.9% for RALP and 85.7% for LP (P=0.25), and both groups achieved a 100% success rate in obstruction resolution. RALP was associated with a longer operative time (212.7±15.4 <i>vs</i>. 185.0±13.8 min, P=0.03) but less blood loss (48.2±9.5 <i>vs</i>. 60.2±11.3 mL, P=0.01). The RALP group also had a trend toward shorter hospital stays (6.5±1.2 <i>vs</i>. 7.7±1.6 days, P=0.07).</p><p><strong>Conclusions: </strong>Both RALP and LP combined with flexible ureteroscopy are effective for treating pediatric UPJO with renal calculi. RALP offers benefits in terms of reduced blood loss and hospital stay, while LP remains a cost-effective alternative. Further studies with larger samples and longer follow-up are needed to validate these findings.</p>","PeriodicalId":23294,"journal":{"name":"Translational pediatrics","volume":"14 6","pages":"1129-1136"},"PeriodicalIF":1.5,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12268701/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Up-regulated vitronectin in Kawasaki disease shock syndrome serves as a potential biomarker.","authors":"Zhimiao Wei, Baoling Bai, Yang Zheng, Hongmao Wang, Mingming Zhang, Qin Zhang, Xiaohui Li","doi":"10.21037/tp-2025-159","DOIUrl":"10.21037/tp-2025-159","url":null,"abstract":"<p><strong>Background: </strong>Kawasaki disease shock syndrome (KDSS) pathogenesis involves an immune inflammatory response related to Kawasaki disease (KD) that damages microvessel endothelial cells, leading to microcirculatory disorders. Its clinical manifestations are characterized by hypotension, poor peripheral perfusion, and a high risk of coronary artery lesions (CALs). Currently, there are few reports on biomarkers based on endothelial cell dysfunction in KDSS. This study aims to identify potential biomarkers of endothelial dysfunction in KDSS at the protein level.</p><p><strong>Methods: </strong>In this study, we recruited age- and sex-matched participants, consisting of KDSS patients, KD patients, and healthy control (HC) children. The inflammatory indicators and cytokines were compared between the KD and KDSS groups. The endothelial barrier function was assessed by dynamically measuring the cell impedance in human coronary artery endothelial cells (HCAECs). Tandem mass tag (TMT)-based proteomics was used to profile the differentially expressed proteins (DEPs) in KDSS plasma. Function and pathway enrichment analyses were performed for related pathways involved in KDSS pathology. Key proteins were validated through Western blotting.</p><p><strong>Results: </strong>Inflammatory cytokines were significantly higher in the KDSS group than in the KD group, and included interleukin-6 (IL-6) (259.37±385.20 <i>vs.</i> 32.96±22.84 pg/mL, P=0.02); soluble interleukin-2 receptor (sIL-2R) (2,529.78±2,016.38 <i>vs.</i> 1,250.50±359.76 pg/mL, P=0.01); and interleukin-10 (IL-10) (63.20±49.91 <i>vs.</i> 12.85±9.47 pg/mL, P=0.005). The KDSS plasma treatment led to earlier and more severe barrier dysfunction in the HCAECs than that seen with the KD plasma treatment. Among the 455 plasma proteins analyzed, 58 were up-regulated and 52 were down-regulated in the KDSS patients. Moreover, 13 DEPs were identified as potential key proteins, and these DEPs primarily associated with cell activation signaling, inflammatory cascades, and endopeptidase activity regulation. Vitronectin was validated to be up-regulated in the KDSS patients.</p><p><strong>Conclusions: </strong>This study provides a potential plasma proteomic profile for KDSS. Vitronectin may serve as a pathogenesis-based diagnostic biomarker for KDSS.</p>","PeriodicalId":23294,"journal":{"name":"Translational pediatrics","volume":"14 6","pages":"1230-1244"},"PeriodicalIF":1.5,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12268671/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of myeloid-derived suppressor cells on retinal epithelial cells in retinopathy of prematurity model.","authors":"Wanyi Huang, Qiaoqun Ou, Yuanchun Liu, Jiaqi Hu, Lina Wang","doi":"10.21037/tp-2024-578","DOIUrl":"10.21037/tp-2024-578","url":null,"abstract":"<p><strong>Background: </strong>Retinopathy of prematurity (ROP) is a proliferative vascular disease with a high incidence rate. Myeloid-derived suppressor cells (MDSCs) are a type of stem cell that possesses remarkable regenerative and reparative capabilities. However, the role of MDSCs as a stem cell therapy in the treatment of ROP remains unclear. Therefore, this study aimed to explore the effects of MDSC therapy on the expression of glial fibrillary acidic protein (GFAP) and vascular endothelial growth factor (VEGF) in a model of ROP, thereby preliminarily assessing the therapeutic potential of MDSCs in ROP.</p><p><strong>Methods: </strong>Bone marrow-derived MDSCs were obtained from an 8-week-old male C57BL/6J mouse, which served as the cell donor. The cells were divided into the model group, which was established by culturing hydrogen peroxide (H₂O₂; 300 µM) with adult retinal pigment epithelial cell line-19 (ARPE-19) alone to create the ROP model; the model + medium group having ARPE-19 and medium; and the model + MDSCs group, having ARPE-19 and MDSCs.</p><p><strong>Results: </strong>The expression levels of GFAP and VEGF in the model + MDSCs group were significantly lower compared to the model group. In the model + MDSCs group, GFAP and VEGF expression levels were significantly reduced. Immunofluorescence analysis revealed a significant increase in GFAP and VEGF expression in the model + medium group. In contrast, the expression levels of GFAP and VEGF in the model + MDSCs group were markedly decreased.</p><p><strong>Conclusions: </strong>Co-culturing with MDSCs significantly reduces the expression levels of GFAP and VEGF in the model group, thereby inhibiting pathological neovascularization, inflammatory responses, and immune-mediated damage. This intervention subsequently improves the pathological processes associated with ROP.</p>","PeriodicalId":23294,"journal":{"name":"Translational pediatrics","volume":"14 6","pages":"1147-1155"},"PeriodicalIF":1.5,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12268656/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}