Tissue & cell最新文献

{"title":"Nucleolin alleviates endotoxemia-induced myocardial dysfunction via inhibiting Drp1-mediated mitochondrial fission","authors":"Ludong Yuan ,&nbsp;Yuting Tang ,&nbsp;Leijing Yin ,&nbsp;Xiaofang Lin ,&nbsp;Pengfei Liang ,&nbsp;Bimei Jiang","doi":"10.1016/j.tice.2025.102964","DOIUrl":"10.1016/j.tice.2025.102964","url":null,"abstract":"<div><h3>Background</h3><div>Our previous study found that nucleolin expression exerted anti-cardiac injury effects by promoting mitochondrial biogenesis; however, it could not explain the increase in mitochondrial fragmentation during myocardial injury. Mitochondrial fragmentation is associated with mitochondrial fission, but it is unknown whether nucleolin regulates mitochondrial fission. Therefore, this study aims to investigate the mechanism by which nucleolin regulates mitochondrial fission in endotoxemia-induced myocardial dysfunction.</div></div><div><h3>Methods</h3><div>Nucleolin myocardial-specific knockout mice were used to construct an endotoxemia-induced myocardial dysfunction model. Mitochondrial membrane potential (MMP), ATP production, Mitotracker Red, Transmission Electron Microscope were measured to assess mitochondrial function. Mitochondria were isolated to observe Drp1 translocation to mitochondria. The expression of pGSK-3β-Tyr216, GSK-3β, pDrp1-Ser637, nucleolin and dynamin-related protein 1 (DNM1L, Drp1) were detected using qRT-PCR and western blot.</div></div><div><h3>Results</h3><div>Following cecum ligation and puncture (CLP) model, cardiac function was impaired, myocardial mitochondrial function declined, mitochondrial morphology became disorganized and fragmented, nucleolin and Drp1 expression was elevated. Myocardial injury and mitochondrial dysfunction were further exacerbated after nucleolin myocardium-specific knockout. Meanwhile, after cellular-level nucleolin interference, it further led to LPS and TNF-α-induced mitochondrial dysfunction and cardiomyocyte damage. Mechanically, nucleolin interference inhibited Drp1 phosphorylation at Ser637 and promoted Drp1 translocation to mitochondria. Myocardial injury caused by nucleolin knockdown was alleviated by the use of P110, an inhibitor of Drp1 mitochondrial translocation.</div></div><div><h3>Conclusion</h3><div>Endotoxemia-induced myocardial dysfunction is accompanied by increased mitochondrial fragmentation. Nucleolin alleviates endotoxemia-induced myocardial dysfunction by enhancing Drp1 phosphorylation at Ser637, inhibiting Drp1 translocation to the mitochondria and mitochondrial fission.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102964"},"PeriodicalIF":2.7,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144115279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The association between XRCC2 and BCORL1 expression with sperm DNA fragmentation index in infertile men candidates undergoing intracytoplasmic sperm injection (ICSI)","authors":"Reza Paknejad ,&nbsp;Farangis Ghassemi ,&nbsp;Masumeh Golestan Jahromi ,&nbsp;Samaneh Zolghadri","doi":"10.1016/j.tice.2025.102982","DOIUrl":"10.1016/j.tice.2025.102982","url":null,"abstract":"<div><h3>Introduction</h3><div>This study aims to investigate the relative gene expression of XRCC2 and BCORL1 in the sperm and its association with DNA fragmentation index (DFI) and sperm parameters in infertile men candidate for fertilization by intracytoplasmic sperm injection (ICSI).</div></div><div><h3>Methods</h3><div>The semen samples from the infertile men candidates were centrifuged using the gradient method to obtain pure sperm. DFI of samples was measured using the sperm chromatin dispersion (SCD) assay. The samples were divided into three groups based on the DFI (with DFI ≤ 15 % (excellent fertility potential), DFI= 15–30 % (good fertility potential), and DFI &gt; 30 % (fair fertility potential). Then, according to WHO criteria, sperm parameters were evaluated. Finally, the expression of XRCC2 and BCORL1 was measured using real-time PCR.</div></div><div><h3>Results</h3><div>This study showed that the expression of XRCC2 in groups with DFI &gt; 30 % was significantly decreased compared to the groups with DFI ≤ 15 % and DFI= 15–30 % (P &lt; 0.05). Furthermore, according to the correlation coefficients data, the mRNA expression level of XRCC2 was correlated positively with fertilization rate and negatively with DFI (p &lt; 0.05). However, there was no association between BCORL1 expression with DFI and other sperm parameters.</div></div><div><h3>Conclusion</h3><div>This investigation revealed that lowering of XRCC2 expression raises sperm DNA damage.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102982"},"PeriodicalIF":2.7,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144194534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Periostin enhances paracrine effect of adipose-derived stem cells on chondrocyte proliferation, adhesion and migration","authors":"Ran Lu,&nbsp;Yong Hou,&nbsp;Xiaogao Wang,&nbsp;Zeyu Guan,&nbsp;Zhonglin Nie,&nbsp;Chaowen Yu,&nbsp;Yong Gao","doi":"10.1016/j.tice.2025.102979","DOIUrl":"10.1016/j.tice.2025.102979","url":null,"abstract":"<div><h3>Background</h3><div>Articular cartilage regeneration after injury remains a difficult clinical problem. Studies have revealed that adipose-derived stem cells (ADSCs) can promote chondrocyte regeneration. This study explored the role of Periostin, a multifunctional extracellular matrix protein, in enhancing the paracrine-mediated effects of ADSCs to promote chondrocyte regeneration.</div></div><div><h3>Material and methods</h3><div>ADSCs were isolated from murine adipose tissue and characterized. Periostin was overexpressed via a lentiviral vector in ADSCs (P-ADSCs). Chondrocytes were isolated and cocultured with P-ADSCs, followed by assessing of cell viability, apoptosis, cell migration and adhesion. In addition, periostin concentrations in the co-culture medium were measured over time by ELISA and detailed densitometric analyses of signaling proteins were performed.</div></div><div><h3>Results</h3><div>Compared with control ADSCs, P-ADSCs significantly enhanced chondrocyte proliferation, reduced apoptosis, and promoted migration and adhesion. ELISA quantification revealed that chondrocytes co-cultured with P-ADSCs showed a significant decrease in pro-inflammatory cytokines of IL-1β, IL-6, IL-18 with less than 0.6 fold-changes, and a significant increase in growth factors of TGF-β, b-FGF, PDGF with more than 4 fold-changes. Moreover, qPCR and immunoblotting demonstrated upregulation of key chondrocyte markers collagen II and Sox9, and activation of FAK/PI3K/Akt/ERK signals.</div></div><div><h3>Conclusions</h3><div>Periostin overexpression in ADSCs enhances their paracrine effect, promoting chondrocyte proliferation, adhesion, migration, and favorable phenotypic marker expression while modulating cytokine secretion. These findings provide a potential strategy for enhancing ADSC-mediated cartilage repair.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102979"},"PeriodicalIF":2.7,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144123493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resolvin D5: A lipid mediator with a therapeutic effect on hepatic steatosis through SIRT6/autophagy","authors":"Do Su Lim ,&nbsp;Sung Ho Ahn ,&nbsp;Hyeon Ji Gwon ,&nbsp;Wonjun Cho ,&nbsp;A.M. Abd El-Aty ,&nbsp;Haci Ahmet Aydemir ,&nbsp;Naveen Sharma ,&nbsp;Soon Auck Hong ,&nbsp;Tae Woo Jung ,&nbsp;Ji Hoon Jeong","doi":"10.1016/j.tice.2025.102980","DOIUrl":"10.1016/j.tice.2025.102980","url":null,"abstract":"<div><div>Resolvin D5 (RD5), a lipid mediator derived from DHA <em>via</em> 5-lipoxygenase signaling, has been shown to resolve inflammation in various disease models. This study aimed to investigate the role of RD5 in the development of hepatic steatosis in individuals with obesity and explore the detailed mechanisms involved. Protein expression was evaluated <em>via</em> Western blot analysis, whereas hepatic lipid deposition was examined <em>via</em> Oil Red O staining and triglyceride quantification. Autophagosomes were detected <em>via</em> MDC staining. Our findings indicated that RD5 treatment normalized lipogenic lipid accumulation, fatty acid uptake, oxidation, apoptosis, and endoplasmic reticulum (ER) stress in palmitate-treated primary hepatocytes. As a cytoprotective signaling pathway, RD5 treatment increased the expression of SIRT6 and autophagy markers, such as those involved in LC3 conversion and p62 degradation. The beneficial effects of RD5 on hepatic lipid metabolism, apoptosis, and ER stress were negated by SIRT6 small interfering RNA or 3-methyladenine, an inhibitor of autophagy. Furthermore, RD5 administration decreased hepatic steatosis, apoptosis, and ER stress in the livers of high-fat diet (HFD)-fed mice. In line with the <em>in vitro</em> results, RD5 treatment elevated SIRT6 and autophagy levels in the livers of HFD-fed mice. These novel findings suggest that RD5 improves hepatic lipid metabolism, apoptosis and ER stress through SIRT6/autophagy signaling, thereby attenuating hepatic steatosis. RD5 may have therapeutic potential for treating nonalcoholic fatty liver disease with minimal side effects.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102980"},"PeriodicalIF":2.7,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144099102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autophagic impairment in endometrial polyps: A potential biomarker and therapeutic target","authors":"Burak Sezgi̇n ,&nbsp;Tuba Edgünlü ,&nbsp;Özgür ilhan Çeli̇k ,&nbsp;Nazlı Can ,&nbsp;Ayşegül Demirtaş Bi̇lgi̇ç","doi":"10.1016/j.tice.2025.102978","DOIUrl":"10.1016/j.tice.2025.102978","url":null,"abstract":"<div><div>Endometrial polyps are a common gynecological condition associated with abnormal uterine bleeding, infertility, and potential malignancies. This study investigated the expression of key autophagy proteins LC3A/B, p62, and Beclin-1 in endometrial polyps. Twenty patients with endometrial polyps and ten healthy controls were enrolled in a prospective randomized controlled trial. Tissue samples were collected via operative hysteroscopy. ELISA and immunohistochemistry were employed to assess the expression of LC3A/B, p62, and Beclin-1. While ELISA results did not reveal significant differences between the two groups, immunohistochemical analysis demonstrated significantly lower levels of LC3A/B, p62, and Beclin-1 in endometrial polyps compared to healthy endometrial tissue (p = 0.041, p = 0.012, and p = 0.003, respectively). These findings suggest that impaired autophagy, as evidenced by reduced levels of these key autophagy proteins, may contribute to the pathogenesis of endometrial polyps. Further research is needed to elucidate the precise mechanisms underlying this association and to explore potential therapeutic implications.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102978"},"PeriodicalIF":2.7,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144123494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hormonal control of the asymmetric uterus of the bat Molossus molossus during pregnancy and lactation: Emphasis on progesterone and estradiol","authors":"Juliana F. Ferraz ,&nbsp;Rodrigo S. de Oliveira ,&nbsp;Cornélio S. Santiago ,&nbsp;Emília M. Soares ,&nbsp;Rejane M. Góes ,&nbsp;Eliana Morielle-Versute ,&nbsp;Sebastião R. Taboga ,&nbsp;Mateus R. Beguelini","doi":"10.1016/j.tice.2025.102974","DOIUrl":"10.1016/j.tice.2025.102974","url":null,"abstract":"<div><div><em>Molossus molossus</em> is an important species of Neotropical insectivorous bat of the family Molossidae. Despite its wide distribution, representativeness, and ecological importance, there are no detailed studies regarding the hormonal control of the female reproductive organs of this species, which presents accentuated morphofunctional asymmetry, with dextro-dominance. Thus, the present study aimed to evaluate the variations in hormone serum levels and in uterine hormonal control of <em>M. molossus</em> during its different reproductive phases, with an emphasis on estradiol and progesterone. Twenty sexually adult females were divided into four sample groups according to the reproductive status — non-reproductive, early pregnancy, late pregnancy and lactating — and subjected to histological, hormonal, and immunohistochemical analysis. The results showed that the uterine dextro-dominance asymmetry of <em>M. molossus</em> is strongly regulated and maintained by the interaction/cross-talk between estradiol and progesterone serum levels with their respective uterine receptors. The right uterine horn presents significantly higher estrogen receptor α and progesterone receptor expression and also greater proliferative activity in the endometrium than the left horn, which favors embryo implantation and development. Significant increases in the estradiol and progesterone serum levels are needed to regulate early pregnancy. The rapid formation of the diffuse portion of the placenta is essential to sustain the high demand for progesterone at the beginning of pregnancy, while the principal portion is not yet formed. Estrogen receptor α and progesterone receptor expression appear to be synchronized to coordinate most of the processes that occur within the uterus of <em>M. molossus</em> throughout the reproductive cycle.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102974"},"PeriodicalIF":2.7,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hedera helix folium extract attenuates methotrexate-induced hepatotoxicity by modulating oxidative stress and inflammatory mediators","authors":"Esra Deniz ,&nbsp;Atilla Topcu ,&nbsp;Aykut Ozturk ,&nbsp;Seda Duman Ozturk ,&nbsp;Kerimali Akyildiz","doi":"10.1016/j.tice.2025.102967","DOIUrl":"10.1016/j.tice.2025.102967","url":null,"abstract":"<div><div>Methotrexate (MTX)-induced hepatotoxicity is linked to oxidative damage and inflammatory processes. <em>Hedera helix folium</em> (HHF) extract protects cells against oxidative damage. We investigated the role of HHF extract in tumor necrosis factor alpha (TNF-α) and interleukin-10 (IL-10)-associated inflammation and oxidative stress in the pathology of MTX-associated liver injury in rats. Forty male rats were divided into one of five equal groups: Control, HHF, MTX, H₁₀₀+MTX and H₂₀₀+MTX. HHF extract was administered via the oral route at 100 mg/kg or 200 mg/kg once daily for seven days, while MTX was administered as a single dose of 20 mg/kg intraperitoneally. Intracardiac blood samples and liver tissue samples were collected at the conclusion of the experiment. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels increased due to MTX. Increased ALT levels were significantly reduced by low-dose HHF and increased AST levels were significantly reduced by high-dose HHF administration. The application of MTX significantly increased malondialdehyde (MDA) and TNF-α levels, while significantly reducing those of glutathione (GSH) and IL-10. High-dose HHF also significantly lowered MDA and TNF-α levels, while significantly increasing those of GSH and IL-10<em>.</em> Histopathological damage findings observed due to MTX were significantly attenuated with high-dose HHF. In addition, the increased caspase-3, p53, and Bcl2 levels caused by MTX decreased with high-dose HHF administration. HHF extract can alleviate liver damage induced by MTX. This extract, which has the ability to reduce damage due to oxidative stress and inflammation, may represent an alternative approach to preventing MTX-induced liver damage.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102967"},"PeriodicalIF":2.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144099103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dorso-ventral skin of flowered racer snake (coluber flourulentus, perreti schatti, 1988): Histological, Keratin immunohistochemical, and ultrastructural characterization","authors":"Ramadan Kandyel ,&nbsp;Saeed Alasmari ,&nbsp;Salem Alharethi ,&nbsp;Bader Albogami ,&nbsp;Abdulrhman Almadiy ,&nbsp;Doaa Gewily ,&nbsp;Fatma A. Madkour ,&nbsp;Mohamed Abumandour","doi":"10.1016/j.tice.2025.102966","DOIUrl":"10.1016/j.tice.2025.102966","url":null,"abstract":"<div><div>Our study, using SEM and histology, aims to characterize the dorsal and ventral skin of <em>Coluber flourulentus</em>, providing new insights into their Keratin immunohistochemical features. The SEM findings revealed overlapping smooth scales on both surfaces, with minor scale morphology variations; the dorsal scales were finger-shaped with a pointed apex, while the ventral scales were flattened with tooth-like edges. Scales on both surfaces reveal two large, spherical-shaped lenticular sensory organs at their apex, dome-shaped receptors/sensilla on the outer surface, and dome-shaped sensilla with dendrites, a transverse cleft, and various pits and pores. Histologically, the micro-ornamentations on the inner-scale surfaces of both surfaces had similar structures with smooth, spiny scales and two layers. The epidermis layer had several layers, including the basal stratum germinativum, α-keratin, the Mesos layer, the β-layer, and the covered Oberhautchen layer. Skin pigmentation varied between both surfaces: the dorsal dermal layer contains melanophores and iridophores, and the ventral dermal layer contains only dark black melanophores under the epidermal layer. Histochemical collagen fiber observations revealed blue-colored fibers in the dermis of dorsal and ventral surfaces, sparse underneath the basement membrane, and visible around pigment cells and muscle bundles. A dark brown reaction was observed in the epidermal cells of both dorsal and ventral surfaces, with high cytokeratin immunolabeling on the ventral surface and moderate cytokeratin immunoreaction on the dorsal surface. In conclusion, scales were crucial for controlling movements and responding to vibrational stimuli from object movements, including those of potential prey or predators.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102966"},"PeriodicalIF":2.7,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IL-37 improves palmitic acid-induced lipid deposition in liver cells by inhibiting ferroptosis to regulate macrophage polarization","authors":"Longqi Zhang,&nbsp;Xinyu Liu","doi":"10.1016/j.tice.2025.102977","DOIUrl":"10.1016/j.tice.2025.102977","url":null,"abstract":"<div><div>Non-alcoholic fatty liver disease (NAFLD), which acts as a predominant contributor to chronic liver disease, remains a pervasive global epidemic. Interleukin-37（IL-37） is documented to have protective effects against various liver diseases. This work focuses on investigating the role and relevant action mechanism of IL-37 in NAFLD. Immunofluorescence assay and Western blot（WB）were used to estimate M1 macrophage markers. For immunofluorescence analysis, images from five randomly selected fields per sample were captured using a confocal microscope (Leica). Fluorescence intensity was quantified by ImageJ software (version 1.53) with background subtraction, and data were normalized to DAPI-positive cells.The lipid Reactive Oxygen Species（ROS）and cell lipid droplet deposition were assessed via BODIPY 581/591 C11 staining and Oil Red O staining. Fe^2 +, triglycerides and cholesterol levels were assessed utilizing appropriate assay kits. WB was adopted for the estimation of proteins associated with ferroptosis and apoptosis. Protein band intensities were quantified using Image Lab software (Bio-Rad) and normalized to β-actin expression. Three technical replicates were analyzed for each biological replicate (n = 3). Our data revealed that IL-37 alleviated PA-stimulated（Palmitic acid-stimulaed）M1 macrophage polarization. It was also identified that IL-37 suppressed lipid accumulation and apoptosis in RAW264.7 cells through inhibiting the polarization of M1 macrophages. Collectively, IL-37 could improve PA-stimulated lipid accumulation and apoptosis in liver cells through suppressing M1 macrophage polarization, which might be mediated by ferroptosis.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102977"},"PeriodicalIF":2.7,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PADI1 aggravates endoplasmic reticulum stress in trophoblast cells by inhibiting the FAK-ERK signaling pathway","authors":"Jianhua Ning ,&nbsp;Songbin Yang ,&nbsp;Fengchun Gao ,&nbsp;Longbin Wang ,&nbsp;Zeyu Lin ,&nbsp;Di Cheng ,&nbsp;Kefeng Fan","doi":"10.1016/j.tice.2025.102976","DOIUrl":"10.1016/j.tice.2025.102976","url":null,"abstract":"<div><div>Preeclampsia (PE) is a multisystemic syndrome of pregnancy that seriously affects maternal and infant healthcare. Here, we identified PADI1 as an abnormally highly expressed gene in PE and investigated its effect on trophoblast cells. According to the analysis results from GEO datasets GSE186257 and GSE143953, PADI1 is highly expressed in the placental samples of PE patients. PADI1 knockdown promoted the growth and migration of trophoblast cells. HTR-8 and Swan-71 cells were treated with 200 nmol/L TG to simulate ERS in vitro. PADI1 knockdown inhibited TG-induced ERS and apoptosis. Mechanistically, PADI1 knockdown downregulated ERS-related factors including IRE1α, XBP1, CHOP, ATF6 and GRP78. The FAK/ERK1/2 signaling pathway has been identified as a downstream target of PADI1 in trophoblast cells. Inhibition of FAK/ERK1/2 effectively hindered the enhancement of cell activity by PADI1 knockdown in TG-treated trophoblast cells. In conclusion, PADI1 was highly expressed in the placental tissues of PE patients. PADI1 knockdown inhibited the ERS-induced apoptosis in trophoblast cells through FAK/ERK1/2 signaling pathway, suggesting the potential role of PADI1 in PE prevention and treatment.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102976"},"PeriodicalIF":2.7,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}