Tissue & cell最新文献

{"title":"Composite systems based on hyaluronic acid and natural polysaccharides for cartilage and bone tissue regeneration: An updated review","authors":"Soumendu Mondal,&nbsp;Trishna Bal","doi":"10.1016/j.tice.2026.103358","DOIUrl":"10.1016/j.tice.2026.103358","url":null,"abstract":"<div><div>The repair of cartilage and bone defects, both structurally and functionally, remains a significant challenge. Traditional treatment methods, such as autogenous and allogeneic bone grafts, face limitations, including issues of availability, cost, immune rejection, and other concerns, making them insufficient to fully address clinical needs. As a result, the integration of biomaterials with tissue engineering strategies has emerged as a promising research direction. Among various materials, hydrogels have attracted considerable attention due to their biological activity, degradability, absorbability, plasticity, and ease of preparation. Hyaluronic acid (HA), a key natural polysaccharide and a major constituent of the extracellular matrix (ECM), has been extensively applied in tissue healing and regeneration because of its excellent biocompatibility, biodegradability, bioactivity, and the availability of reactive functional groups for modification. In particular, HA-composed hydrogels, compared to typical hydrogels, provide a highly adaptable structure and a tissue-mimetic microenvironment that closely resembles the ECM, thereby supporting and enhancing tissue repair and regeneration. In this review, we have discussed the mechanisms through which HA-based hydrogels promote ECM formation in cartilage and bone, their combination with other natural polymers, hyaluronic acid composited injectable hydrogel and the application of 3D bio-printed HA hydrogels for effective cartilage and bone regeneration.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"100 ","pages":"Article 103358"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146100716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immortalized periodontal ligament mesenchymal cells retain undifferentiation, proliferation and osteogenic characteristics","authors":"Ana Carolina Bontempi ,&nbsp;Letícia Faustino Adolpho ,&nbsp;Anne Caroline Teles Campos de Carvalho ,&nbsp;Natália Bispo de Sousa ,&nbsp;Taís Browne de Miranda ,&nbsp;Matheus Kury ,&nbsp;Denise Carleto Andia","doi":"10.1016/j.tice.2026.103326","DOIUrl":"10.1016/j.tice.2026.103326","url":null,"abstract":"<div><div>Periodontal ligament-derived mesenchymal stem cells (PDLCs) are accessible and multipotent, with potential in regenerative therapies. However, their limited replicative capacity restricts long-term <em>in vitro</em> studies and hinders deeper understanding of mineralization mechanisms. To overcome this, we established an immortalized PDLC line (iPDLCs) by introducing the human telomerase reverse transcriptase (<em>hTERT</em>) gene via lentiviral transduction. We compared iPDLCs to primary PDLCs in terms of phenotype, proliferation, and osteogenic differentiation. Surface markers (CD105, CD166, CD34) were assessed by flow cytometry, while gene expression of <em>hTERT</em>, <em>OCT4</em>, <em>NANOG</em>, <em>MAPK14</em>, <em>YAP1</em>, <em>CREB1</em>, <em>RUNX2</em>, <em>SP7</em>, and <em>ALPL</em> was analyzed by RT-qPCR. ALPL activity and Alizarin Red staining were used to evaluate mineralization. iPDLCs showed a 2,300-fold increase in <em>hTERT</em> expression and maintained mesenchymal features, with high CD105/CD166 and low CD34 expression, as well as elevated <em>OCT4</em> and <em>NANOG</em> levels. Upon osteogenic induction, iPDLCs upregulated proliferation-related genes (<em>MAPK14</em>, <em>YAP1</em>, <em>CREB1</em>) and showed early and enhanced expression of osteogenic markers (<em>RUNX2</em>, <em>SP7</em>, <em>ALPL</em>), particularly on day 7 (p ≤ 0.05 vs. PDLCs). Strong correlations were found between <em>YAP1</em>, <em>RUNX2</em>, and <em>SP7</em> expression on days 7 and 10. Despite this early gene activation in iPDLCs, mineral deposition was greater in PDLCs at day 28 (p ≤ 0.05), confirmed by ALPL activity. These findings suggest that iPDLCs retain their undifferentiated state, proliferation capacity, and mineralization potential, while offering a reliable and long-lasting model for studying the molecular mechanisms involved in osteogenic differentiation of PDLCs.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"100 ","pages":"Article 103326"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145969315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Houpu Sanwu decoction alleviates rat intraperitoneal adhesion by regulating macrophage polarization and inhibiting inflammatory responses","authors":"Mei Liu ,&nbsp;Quanxin Chen ,&nbsp;Yan Cui ,&nbsp;Tianming Wang ,&nbsp;Qun Li ,&nbsp;Zhihang Wu ,&nbsp;Richeng Li ,&nbsp;Yuan Tang ,&nbsp;Haibang Pan","doi":"10.1016/j.tice.2026.103371","DOIUrl":"10.1016/j.tice.2026.103371","url":null,"abstract":"<div><h3>Background</h3><div>Intraperitoneal adhesion frequently occurs after abdominal surgery, substantially affecting patients’ quality of life. This study evaluated the therapeutic effect of Houpu Sanwu decoction on intraperitoneal adhesion and its possible molecular mechanism.</div></div><div><h3>Methods</h3><div>Thirty-six male Sprague Dawley rats were randomly assigned to sham, control, fluvastatin and low-, medium-, and high-concentration Hopu Sanwu decoction groups. All groups, except for the sham group, underwent abdominal adhesion modeling. Hematoxylin and eosin staining was conducted to observe the pathological changes in peritoneal tissue. Masson staining was used to observe the development of collagen fibers within the adhesive tissues. The protein levels of MCP-1 in adhesion tissues were detected using immunohistochemistry. The serum levels of IL-1, IL-6, TNF-α, IL-4, and IL-10 were detected using ELISA. RT-PCR and western blotting were utilized to identify the expression levels of the SOCS/JAK2/STAT/PPAR-γ pathway.</div></div><div><h3>Results</h3><div>The occurrence of intraperitoneal adhesion decreased in the low-, medium-, and high-concentration Hopu Sanwu decoction groups compared with the control group, with the most significant decrease observed in the medium-concentration group (P &lt; 0.05). Following Hopu Sanwu decoction treatment, MCP-1 protein expression in adhesive tissue significantly decreased (P &lt; 0.05). Furthermore, markers associated with M1-type macrophages, such as IL-1, IL-6, and TNF-α, exhibited decreased expression levels, whereas markers of M2-type macrophages, such as IL-4 and IL-10, increased post-Hopu Sanwu decoction treatment. Additionally, Hopu Sanwu decoction can activate the SOCS1/STAT6/PPAR-γpathway, inhibit the SOCS3/JAK2/STAT1 pathway, and promote the polarization of macrophages toward M2.</div></div><div><h3>Conclusions</h3><div>These results suggest that Hopu Sanwu decoction is effective in alleviating intraperitoneal adhesion, possibly by inducing M2-type macrophage polarization and modulating the SOCS/JAK2/STAT/PPAR-γ pathway.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"100 ","pages":"Article 103371"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146166638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Astaxanthin-loaded chitosan nanoparticles attenuate chlorpyrifos-induced nephrotoxicity via activation of the Nrf2/HO-1 axis and suppression of oxidative, inflammatory, and apoptotic pathways","authors":"Ahmad A. Obaid ,&nbsp;Mazen M. Ghaith ,&nbsp;Ahmad Najem Alshammari ,&nbsp;Ekramy M. Elmorsy ,&nbsp;Manal S. Fawzy ,&nbsp;Neven A. Ebrahim ,&nbsp;Hamada S. Salem ,&nbsp;Nashwa Mostafa Hussein","doi":"10.1016/j.tice.2026.103332","DOIUrl":"10.1016/j.tice.2026.103332","url":null,"abstract":"<div><h3>Background</h3><div>Chlorpyrifos was shown to cause oxidative, inflammatory, and apoptotic renal damage. The natural Astaxanthin (ASX) exhibits nephroprotective effects, however its limited bioavailability hinders its therapeutic potential. Chitosan nanoparticles were shown to enhance bioavailability and therapeutic delivery of the natural compounds.</div></div><div><h3>Objective</h3><div>This study investigated the renoprotective effects of astaxanthin-loaded chitosan nanoparticles (ASX-CNPs) compared with crude astaxanthin (ASX) against chlorpyrifos (CPF)-induced nephrotoxicity in male Wistar rats.</div></div><div><h3>Methods</h3><div>Ninety rats (235 g) were randomly assigned into six groups (n = 15) and orally treated for 60 days with saline, ASX (40 mg/kg), ASX-CNPs (40 mg/kg), CPF (10 mg/kg), or combinations of CPF with ASX or ASX-CNPs. ASX-CNPs were characterized by transmission electron microscopy and dynamic light scattering, revealing uniform spherical nanoparticles with high stability and an encapsulation efficiency of 84.72 %. Oxidative, inflammatory, and apoptotic pathways were evaluated via different assays</div></div><div><h3>Results</h3><div>CPF exposure significantly impaired renal function, elevating blood urea, creatinine, uric acid, cystatin C, and NGAL levels, while promoting oxidative stress, lipid peroxidation, and DNA damage. CPF also suppressed the Nrf2/HO-1 antioxidant pathway, triggered inflammation and nitrosative stress, and disrupted apoptotic balance by increasing Bax and Caspase-3 while decreasing Bcl-2 expression. Histopathological and ultra-structural analysis confirmed severe renal alterations, including glomerular contraction, structurally disrupted mitochondria, vascular congestion, and tubular degeneration. Co-treatment with ASX-CNPs markedly ameliorated biochemical, oxidative, inflammatory, nitrosative, and apoptotic disturbances, restoring renal morphology close to normal. In contrast, crude ASX provided partial protection, with less pronounced effects on antioxidant enzyme activities, cytokine levels, and tissue architecture. The superior efficacy of ASX-CNPs highlights the advantages of nanoparticle delivery in enhancing bioavailability, stability, and cellular uptake compared with the conventional form.</div></div><div><h3>Conclusion</h3><div>These findings indicate that ASX-CNPs represent a promising nanotherapeutic strategy for preventing CPF-induced kidney injury and demonstrate the enhanced protective potential of nanoformulations over their crude counterparts</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"100 ","pages":"Article 103332"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146039617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SURF4 inhibits autophagy to enhance lung adenocarcinoma progression and stemness through Hedgehog pathway regulation","authors":"Junzheng Zhou ,&nbsp;Shiwei Nie ,&nbsp;Xiaodong Zheng ,&nbsp;Xudong Wei ,&nbsp;Jinrui Zhang ,&nbsp;Xiaojuan Shen ,&nbsp;Weimin Zhang","doi":"10.1016/j.tice.2026.103351","DOIUrl":"10.1016/j.tice.2026.103351","url":null,"abstract":"<div><h3>Background</h3><div>Drug resistance, metastasis, and recurrence are major contributors to the poor survival rates of lung adenocarcinoma (LUAD) patients. Studies suggest that tumor stemness is a key driver of drug resistance, recurrence, and metastasis. Although Surfeit locus protein 4 (SURF4) has been shown to promote tumor cell stemness, the molecular mechanisms by which SURF4 regulates LUAD growth and stemness remain elusive.</div></div><div><h3>Methods</h3><div>Differentially expressed genes were identified using the TCGA-LUAD database to compare LUAD tissues with adjacent normal tissues, followed by experimental validation. qRT-PCR, CCK-8, and flow cytometry assays were used to assess the functional impact of SURF4. Tumor stemness was evaluated by sphere formation assays and Western blotting. In addition, GSEA combined with cellular experiments was employed to validate the mechanism underlying SURF4-mediated regulation of LUAD growth and stemness.</div></div><div><h3>Results</h3><div>There was abnormal upregulation of SURF4 in LUAD tissues and cells. Knockdown of SURF4 enhanced autophagy and suppressed LUAD cell proliferation and stemness. Mechanistically, SURF4 was found to activate the Hedgehog pathway by facilitating Sonic hedgehog (Shh) transport, thereby inhibiting autophagy. Rescue experiments confirmed that SURF4's effects on LUAD growth and stemness were primarily mediated through this mechanism.</div></div><div><h3>Conclusion</h3><div>SURF4 promotes LUAD proliferation and maintains tumor stemness by suppressing autophagy through Hedgehog pathway activation.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"100 ","pages":"Article 103351"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146120219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Indispensable amino acids vastly protect against CCl4-mediated aggravated liver cirrhosis and immunotoxicity","authors":"Zeinab Al-Amgad ,&nbsp;Elsayed S.I. Mohammed ,&nbsp;Mariam M. Jad ,&nbsp;Hend Al-Amgad ,&nbsp;Enas A. Noseer ,&nbsp;Amany Ahmed Abd El-Aziz ,&nbsp;Asmaa W. Basher ,&nbsp;Fatma A. Madkour","doi":"10.1016/j.tice.2026.103357","DOIUrl":"10.1016/j.tice.2026.103357","url":null,"abstract":"<div><div>Without doubt, liver injury and immunotoxicity are patently interconnected. The liver's role as a leading regulator of the immune system is compromised in advanced liver illness, which may cause immune dysfunction. The liver disorders, when they deteriorate the immunity, making it more vulnerable to systemic damage, while certain chemicals can induce liver injury through direct immune responses. Branch-chain amino acids (BCAAs) are indispensable amino acids immensely recommended as pharmacological nutrients for those with chronic liver diseases to restrict the development of systemic complications caused by toxicants. The aim of this dietary treatment for CCl4-induced liver immunotoxicity and remote disorders is to recruit branched-chain amino acids enriched in the protein diet to achieve therapeutic effectiveness. In the current design, 40 male Wister rats (n = 10 per group) were randomly divided into four equal groups: The study included four groups: control, CCl4, CCl4 +BCAAs, and CCl4 +protein diet. Histomorphological analysis was conducted using toluidine blue and Masson's trichrome stains, and immunohistochemical assessment employed the caspase-3 gene as a marker, and standard diagnostic methods were applied to evaluate liver biomarkers. Poisoning by CCl4 emphasized a significant increase in liver transaminases, and unlimited histomorphological deteriorations elicited by severe fibrosis. Significant apoptosis was also demonstrated by the elevated apoptotic expression of caspase-3 in the target tissues. Concurrent supplementation of BCAAs with dietary protein following CCl4 administration returned histomorphological and immunohistochemical alterations to normal architecture and downregulated caspase-3 expression, which correlated with decreased transaminases. Fundamentally proposed that CCl4-induced rats administered a protein diet mainly of plant origin, concomitant with BCAAs, were immunologically effective not only in reducing liver cirrhosis but also as coadjuvant treatment for specific lesions.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"100 ","pages":"Article 103357"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146133048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomes from APCs ameliorate human skin fibroblast senescence via p53/p21 signaling pathway","authors":"Yong-Chao Zhang ,&nbsp;Mei-Shui Wang ,&nbsp;Biao Wang ,&nbsp;Cong-yi Wang ,&nbsp;Yuan-Jun Qin","doi":"10.1016/j.tice.2026.103363","DOIUrl":"10.1016/j.tice.2026.103363","url":null,"abstract":"<div><div>Exosomes have emerged as important resources in skin regenerative medicine. However, only a limited number of studies have demonstrated the anti-aging effects of progenitor cell–derived exosomes. In addition, the development of novel effective progenitor cell–based therapies is crucial for the treatment of skin aging. In this study, the viability and proliferation of human adipose-derived progenitor cells (APCs) from young (18–25 years) and old (60–67 years) donors were compared. Exosomes derived from young (yAPC-Exos) and old (oAPC-Exos) APCs were collected and characterized, and their effects on senescent human dermal fibroblasts (HDFs), as well as the underlying molecular mechanisms, were investigated. The proliferation capacity of aged APCs was significantly reduced. Both yAPC-Exos and oAPC-Exos promoted HUVEC migration and tube formation, as well as HDF migration. Exosome treatment decreased intracellular reactive oxygen species levels and alleviated aging-associated phenotypes in senescent HDFs. These effects occurred primarily through p21 and p53 downregulation and SIRT1 upregulation. Notably, yAPC-Exos exerted more pronounced anti-senescent effects than oAPC-Exos. Taken together, yAPC-Exos may represent an effective therapeutic strategy for aging-related skin pathologies and cosmetic applications.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"100 ","pages":"Article 103363"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146143551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adenosine A₂A receptor as a dual-acting molecular switch: Glial morphological changes and neurovascular tissue remodeling in neuroinflammation and neurodegeneration","authors":"Heba Ibrahim Abd El-Moaty ,&nbsp;Ahmed Sameh ,&nbsp;Sameh Saber ,&nbsp;Rabab S. Hamad ,&nbsp;Elsayed A. Elmorsy ,&nbsp;Norah Suliman Alsoqih ,&nbsp;Alshaimaa A. Farrag ,&nbsp;Hanan Eissa ,&nbsp;Attalla F. El-kott ,&nbsp;Sally Negm ,&nbsp;Mohammed A. AlShehri ,&nbsp;Mohamed A.M. Ali ,&nbsp;Waseem Ali Hasan ,&nbsp;Ahmed Gaafar ,&nbsp;Heba S. Khalifa ,&nbsp;Essam H. Ibrahim ,&nbsp;Kareem Morsy ,&nbsp;Fatma Elnaghy","doi":"10.1016/j.tice.2026.103389","DOIUrl":"10.1016/j.tice.2026.103389","url":null,"abstract":"<div><div>Neuroinflammation appears in a variety of neurological disorders, including multiple sclerosis (MS), Parkinson's disease (PD), Alzheimer's disease (AD), and amyotrophic lateral sclerosis. The adenosine A₂A receptor (A₂AR), a Gs protein-coupled receptor that affects cAMP signaling and downstream kinases like PKA, CREB, and NF-κB, is one of the primary regulators of this process. Context-dependent effects of A₂AR activation include lowering acute inflammation and promoting neuronal survival when stimulated moderately, but increasing glial activation and cytokine production when overexpressed over an extended period of time. In microglia and astrocytes, A₂AR signaling regulates inflammatory pathways mediated by NF-κB and MAPK, affecting oxidative stress, blood-brain barrier (BBB) stability, and excitotoxicity. Acute or transient (short-term) A₂AR activation, on the other hand, increases the production of anti-inflammatory cytokines like IL-10 and enhances neurotrophic support through BDNF. A₂AR antagonists, including istradefylline and SCH58261, may reduce microglial triggering and have neuroprotective benefits, according to clinical and experimental data. The context-dependent activity of the receptor is shown by the fact that total receptor blockage interferes with adaptive immune control. Therefore, the therapeutic challenge is to carefully modify A₂AR signaling in particular cell populations, specifically targeting astrocytic or microglial receptors while maintaining the peripheral immunoregulatory activities. The dual regulatory role of A₂AR in neuroinflammation is summarized in this review along with its molecular mechanisms, disease-specific actions, and therapeutic significance. Developing next-generation neuroprotective strategies that reduce A₂AR signaling's pro-inflammatory and neurotoxic effects while preserving its beneficial homeostatic effects will require an understanding of the temporal and cell-specific dynamics of this signaling.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"100 ","pages":"Article 103389"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146189430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acellular dental pulp and allogeneic cells for strategic pulp regeneration","authors":"Nora Sakina Mohd Noor ,&nbsp;Xin-Jieh Lam ,&nbsp;Ghee-Seong Lim ,&nbsp;May-Nak Lau ,&nbsp;Mang-Chek Wey ,&nbsp;Abilashini Rajendran ,&nbsp;Khong-Lek Then ,&nbsp;Nazmul Haque ,&nbsp;Noor Hayaty Abu Kasim ,&nbsp;Vijayendran Govindasamy ,&nbsp;Noor Hayati Azami","doi":"10.1016/j.tice.2026.103369","DOIUrl":"10.1016/j.tice.2026.103369","url":null,"abstract":"<div><div>Regenerative endodontics aims to restore the damaged pulp-dentin complex, moving beyond revascularization to functional tissue regeneration, which includes innervation. While dental pulp stem cells (DPSCs) have been widely studied, their clinical application is hindered by low cell yield and reduced regenerative capacity in late passages. This study investigates the feasibility of using Wharton’s Jelly mesenchymal stem cells (WJ-MSCs) as an alternative allogenic cell source, combining them with a decellularized human dental pulp (dDP) scaffold as a platform crucial for functional pulp regeneration. Dental pulp tissues were successfully decellularized, confirmed by DNA and hydroxyproline quantification, and histological and SEM analyses showing robust preservation of collagen types I and III with efficient cell removal. WJ-MSCs were then successfully repopulated onto the dDP scaffolds, demonstrating cellular attachment and migration into the ECM. Repopulated dDP constructs exhibited a propensity for osteogenic and odontogenic differentiation, evidenced by Alizarin Red staining for mineral deposition and upregulated gene expressions of osteogenic (DLX5, OSTERIX) and odontogenic (DSPP) markers. Furthermore, neurogenic marker NCAM was upregulated, whereas inhibitory genes (TWIST2, MSX1), angiogenic markers (VE-CADHERIN, VEGF), and immune regulatory marker IDO1 were downregulated following differentiation. Overall, the findings suggest the feasibility of combining WJ-MSCs with dDP scaffolds as a novel approach for functional pulp regeneration, offering a promising pathway for future clinical translation in regenerative endodontics.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"100 ","pages":"Article 103369"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146189721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scanning electron microscopic insights into the oropharyngeal cavity (tongue, laryngeal mound, and palate) of the Eurasian stone-curlew (Burhinus oedicnemus saharae): Anatomical adaptations to its feeding habits","authors":"Mohamed M.A. Abumandour,&nbsp;Basma G. Hanafy","doi":"10.1016/j.tice.2026.103354","DOIUrl":"10.1016/j.tice.2026.103354","url":null,"abstract":"<div><div>This study presents the first ultrastructural analysis of the Eurasian stone-curlew's oropharyngeal cavity through scanning electron microscopy (SEM) and gross anatomical observations, focusing on the tongue, laryngeal mound, and palate to understand structural adaptations related to feeding habits. The short tongue was divided into apex, body, and root, each exhibiting distinct anatomical and morphometric features. SEM showed the apex had a pointed nail, scales, and is bordered laterally by 2–3 membranes. The body is divided into a rostral papillary area and a caudal non-papillary area. The papillary system is restricted to the dorsal surface, especially on the apex and the rostral part of the body. Within this papillary area, there are three distinct filiform papillae types: thick papillae at the apex, elongated, pointed papillae along the lateral margins of the body, and broad, scale-like papillae at the median body region, along with the conical papillae on the papillary crest. Meanwhile, the caudal body part and root were devoid of papillae, yet featured numerous rounded salivary gland openings. The papillary crest had 14 small, pointed papillae arranged symmetrically on each side, with two large papillae at their lateral edges, each dividing into three papillae. The laryngeal mound had a notable glottis and a W-shaped papillary arrangement. The palatine region consists of rostral, middle, and caudal parts, with 2, 8, and 1 ridge(s), respectively. The choana is divided into a papillary rostral part and a non-papillary caudal part. The infundibulum had a median opening with no papillae and multiple sphenopterygoid gland openings.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"100 ","pages":"Article 103354"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146133022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}