Ultrasonic irradiation for ZnO NPs Synthesis and their hepatoprotective effect against acetaminophen induced hepatic toxicity in albino rats: Biochemical, physiological, and histopathological assessments

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-08-20 DOI:10.1016/j.tice.2025.103095

Yasmine A. Mansour , Fady Sayed Youssef , Shaymaa Hussein , Hanan F. AbdEl_Halim , Sameh H. Ismail , Gehad G. Mohamed , Abdelfattah M. Abdelfattah , Rasha M.M. Ezz-Eldin , Heba H. Mahboub , Sherin Ibrahim

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引用次数: 0

Abstract

The present novel trial assesses the prophylactic influence of ZnO NPs in comparison to silymarin against liver damage induced by acetaminophen (APAP). Forty albino rats were allocated into 4 groups (n = `10 rats/ group). Group I (Control), was orally administered 0.9 % NaCl for 21 days. Group II (exposed to APAP) received distilled water (1 ml/kg per day) via oral gavage for 19 successive days followed by APAP (600 mg/kg, PO) twice daily for 2 days. Group III (APAP+ Silymarin) was orally administered silymarin at the dose of 200 mg/kg daily for 19 days followed by APAP (600 mg/kg) via oral gavage twice daily for 2 successive days. Group IV (APAP+ ZnO NPs) was orally given ZnO NPs at the dose of 100 mg/kg daily for 19 days followed by (600 mg/kg/ twice daily) APAP orally for 2 successive days. APAP exhibited a substantial elevation of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, nitric oxide and malondialdehyde levels. Meanwhile, APAP markedly decreased catalase, SOD, GST and TAC levels. A significant elevation in DNA damage inside hepatocytes was noticed. APAP induced many histopathological changes. Conversely, the prophylactic use of ZnO NPs inhibited the elevation of AST, ALT, ALP, NO and MDA as well as the decline of catalase, SOD, GST and TAC levels induced by APAP toxicity compared to group III. Marked improvement of the architecture of hepatic tissue was noticed in group IV. In conclusion, ZnO-NPs were more actual in ameliorating APAP hepatotoxicity compared to silymarin via antioxidant and antiapoptotic pathways.

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超声照射对氧化锌NPs合成及其对白化大鼠对乙酰氨基酚肝毒性的保护作用：生化、生理和组织病理学评价

本研究评估了氧化锌NPs与水飞蓟素对对乙酰氨基酚（APAP）引起的肝损伤的预防作用。40只白化大鼠分为4组（n = ’10只/组）。第一组（对照组）口服0.9% % NaCl，治疗21 d。II组（APAP暴露组）连续19天灌胃蒸馏水（1 ml/kg /天），随后给予APAP(600 mg/kg， PO)，每天2次，连续2天。第三组（APAP+水飞蓟素）给予水飞蓟素200 mg/kg / d口服，连续19 d，随后APAP 600 mg/kg / d灌胃，每天2次，连续2 d。IV组（APAP+ ZnO NPs）以100 mg/kg / d / d的剂量口服ZnO NPs，连续19 d，然后口服600 mg/kg/ 2次/ d的APAP，连续2 d。APAP表现出谷丙转氨酶、天冬氨酸转氨酶、碱性磷酸酶、一氧化氮和丙二醛水平的显著升高。同时，APAP显著降低过氧化氢酶、SOD、GST和TAC水平。肝细胞内DNA损伤显著升高。APAP诱导了许多组织病理改变。相反，与III组相比，预防性使用ZnO NPs可抑制APAP毒性引起的AST、ALT、ALP、NO和MDA的升高，以及过氧化氢酶、SOD、GST和TAC水平的下降。IV组肝组织结构明显改善。由此可见，与水飞蓟素相比，ZnO-NPs通过抗氧化和抗凋亡途径改善APAP肝毒性更实际。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.