Tissue & cell最新文献

AP3M2: A key regulator from the nervous system modulates autophagy in colorectal cancer AP3M2：神经系统的一个关键调节因子调节结直肠癌的自噬作用

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-10-31 DOI: 10.1016/j.tice.2024.102593

Maguie El Boustani , Nayla Mouawad , Monah Abou Alezz

引用次数: 0

Phosphocreatine ameliorates hepatocellular apoptosis mediated by protecting mitochondrial damage in liver ischemia/reperfusion injury through inhibiting TLR4 and Agonizing Akt Pathway 磷酸肌酸通过抑制 TLR4 和激动 Akt 通路，改善肝缺血再灌注损伤中由保护线粒体损伤介导的肝细胞凋亡

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-10-30 DOI: 10.1016/j.tice.2024.102599

Fu Han Wang , Eskandar Qaed , Waleed Aldahmash , Mueataz A. Mahyoub , Zhongyuan Tang , Peng Chu , Ze Yao Tang

{"title":"Phosphocreatine ameliorates hepatocellular apoptosis mediated by protecting mitochondrial damage in liver ischemia/reperfusion injury through inhibiting TLR4 and Agonizing Akt Pathway","authors":"Fu Han Wang , Eskandar Qaed , Waleed Aldahmash , Mueataz A. Mahyoub , Zhongyuan Tang , Peng Chu , Ze Yao Tang","doi":"10.1016/j.tice.2024.102599","DOIUrl":"10.1016/j.tice.2024.102599","url":null,"abstract":"<div><div>Hepatic ischemia/reperfusion (HI/R) presents significant challenges in surgical liver transplantation and hepatic ischemic shock, with few effective clinical preventive measures available. This study explores the potential protective effects and underlying mechanisms of phosphocreatine (PCr) in the context of HI/R. We established an in vitro ischemia/reperfusion model using hepatocellular carcinoma HepG2 cells and normal liver L02 cells. For in vivo assessments, C57BL/6 mice were subjected to the HI/R model to evaluate the impact of PCr on liver protection. PCr pretreatment significantly improved liver cell survival rates, maintained mitochondrial membrane potential (MMP), reduced apoptosis, and alleviated oxidative damage and inflammatory responses. Importantly, PCr exerted its protective effects by downregulating TLR4 and activating the Akt signaling pathway, which suppressed inflammation, mitigated oxidative stress, inhibited apoptosis, and modulated key biomarkers, including ALT, AST, IL-6, IL-1β, TNF-α, SOD, MDA, and reactive oxygen species (ROS). Western blot analyses demonstrated PCr's anti-inflammatory effects through the regulation of UCP2, Cyp-D, Cyt-C, and PGC-1α, thereby preserving mitochondrial structure and function, maintaining MMP, and regulating membrane pores. Transmission electron microscopy further highlighted PCr's role in sustaining mitochondrial integrity. In conclusion, our findings suggest that PCr helps maintain mitochondrial homeostasis by intervening in the TLR4 inflammatory pathway and activating the Akt signaling pathway, ultimately reducing liver injury. This study offers new insights and potential treatment strategies for HI/R, providing valuable guidance for future clinical applications.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142560561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the involvement and mutual interaction of wbp2 and yap in embryogenesis with an emphasis on liver function in zebrafish embryos 以斑马鱼胚胎的肝功能为重点，评估 wbp2 和 yap 在胚胎发育过程中的参与和相互作用

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-10-29 DOI: 10.1016/j.tice.2024.102600

Nikita Lykov , Huiling Wang , Mogellah John Panga, Zhanxiang Du, Ziyi Chen, Shitian Chen, Lin Zhu, Ye Zhao

{"title":"Evaluating the involvement and mutual interaction of wbp2 and yap in embryogenesis with an emphasis on liver function in zebrafish embryos","authors":"Nikita Lykov , Huiling Wang , Mogellah John Panga, Zhanxiang Du, Ziyi Chen, Shitian Chen, Lin Zhu, Ye Zhao","doi":"10.1016/j.tice.2024.102600","DOIUrl":"10.1016/j.tice.2024.102600","url":null,"abstract":"<div><div>The Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) play complex roles in liver health, influencing processes such as fibrosis, cancer development, and regeneration. WW domain binding protein-2 (WBP2) primarily enhances the co-translational activity of YAP/TAZ, which is crucial for the progression of liver diseases. Despite existing knowledge, the specific functions of WBP2 and its interactions with YAP remain inadequately understood. This study investigates the expression levels of WBP2 in zebrafish embryos and its molecular interaction with YAP. We employed morpholino-mediated knockdown of <em>wbp2</em> and <em>yap</em>, followed by assessments of liver histology, immunofluorescence, and co-immunoprecipitation. Subsequently, RNA sequencing analyses were conducted to elucidate the signaling pathways and mechanisms underlying the interplay between YAP and WBP2 in liver injury. Our findings highlight the significant interaction between WBP2 and YAP, emphasizing their potential as therapeutic targets for liver diseases.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142560563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dose and time dependent morphodynamic changes in the ovary of nano-nickel treated rats A SEM study 纳米镍处理过的大鼠卵巢中与剂量和时间相关的形态变化 SEM 研究

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-10-29 DOI: 10.1016/j.tice.2024.102598

Meenu Singh, Yeshvandra Verma, SV S. Rana

{"title":"Dose and time dependent morphodynamic changes in the ovary of nano-nickel treated rats A SEM study","authors":"Meenu Singh, Yeshvandra Verma, SV S. Rana","doi":"10.1016/j.tice.2024.102598","DOIUrl":"10.1016/j.tice.2024.102598","url":null,"abstract":"<div><h3>Aims</h3><div>Present study demonstrates dose and time dependent effects of NiONPs (<30 nm) on the ovaries of Wistar rat.</div></div><div><h3>Methods</h3><div>Female rats were gavaged NiONPs or NiOMPs (5 mg/kg b.w.) for 24 h, 15 days and 30 days, euthanized and ovaries thus removed were analyzed for nickel bioaconcentration and processed for scanning electron microscopy. Serum samples were analyzed to compare the effects of nickel nano & microparticles on progesterone and estradiol values.</div></div><div><h3>Results</h3><div>Results confirmed the bioaccumulation of Ni in ovarian tissue. Its concentration was higher in NiONPs treated rats than NiOMPs treated rats. Progesterone level increased whereas estradiol values decreased in NiONPs and NiOMPs treated rats. SEM results also exhibited dose dependent effects on the morphology of corpoluteal complex. The structural changes varied from formation of blebs to distorted microvilli and germinal epithelium.</div></div><div><h3>Conclusion</h3><div>It is hypothesized that NiONPs/NiOMPs are biodegraded into smaller fragments that conjugate with amino acids and or alter downstream signaling pathways, generate ROS and modulate protein structure activity relationships. Finally, these processes manifest into morphological alterations in the ovary. Biopersistence of nickel in female reproductive system may compromise with fertility and reproductive performance of exposed population.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142560562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative microanatomy and histology of spinal and cerebral veins: Implications for dural arteriovenous fistula clinical presentations 脊髓静脉和大脑静脉的显微解剖学和组织学比较：对硬脑膜动静脉瘘临床表现的启示

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-10-28 DOI: 10.1016/j.tice.2024.102597

Etienne Lefevre , Mégane Le Quang , Vincent Jecko , Maxime Nogues , Dominique Liguoro , Franck Bielle , Paul Roblot

{"title":"Comparative microanatomy and histology of spinal and cerebral veins: Implications for dural arteriovenous fistula clinical presentations","authors":"Etienne Lefevre , Mégane Le Quang , Vincent Jecko , Maxime Nogues , Dominique Liguoro , Franck Bielle , Paul Roblot","doi":"10.1016/j.tice.2024.102597","DOIUrl":"10.1016/j.tice.2024.102597","url":null,"abstract":"<div><div>Cranial dural arteriovenous fistulas (DAVFs) that display cortical venous drainage are at risk of hemorrhage, unlike spinal DAVFs, which seldom bleed. The underlying mechanism for this difference is poorly understood. We hypothesized that cerebral veins are more fragile than spinal veins due to differences in histologic compositions. Thus, spinal and cerebral veins from five formalin-fixed human cadavers were examined through macroscopic and histological analysis, using hematoxylin, eosin and safran (HES), and orcein stains to compare them. Twenty-four cerebral veins and thirteen spinal veins were analyzed. The mean diameter of the cerebral veins was 1.02 ± 0.59 mm, while that of spinal veins was 0.52 ± 0.26 mm (p = 0.003). The mean thickness of cerebral veins was similar to their spinal counterparts (0.09 ± 0.07 mm vs 0.06 ± 0.02 mm; p = 0.12). The mean diameter-to-thickness ratio was 13.76 ± 6.05 mm for cerebral veins and 10.06 ± 7.23 mm for spinal veins (p = 0.023). In most of the analyzed vessels, the venous wall was composed of endothelial cells resting on layers of smooth muscle, separated by elastica lamina. Cerebral and spinal veins exhibit distinct calibers while maintaining comparable wall thicknesses, resulting in a greater diameter-to-thickness ratio for cerebral veins compared to spinal veins. This difference may after their resistance to pressure. Furthermore, variations in the transparietal pressure gradient between cranial and spinal subarachnoid space, along with differences in arterial blood flow through the fistulous veins, might contribute to the observed differences in clinical presentation.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142554231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ω-9 monounsaturated fatty acids in Sapindus mukorossi seed oil enhance calcium deposition expression of Wharton’s jelly mesenchymal stem cells 无患子种子油中的ω-9 单不饱和脂肪酸可增强沃顿果冻间充质干细胞的钙沉积表达

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-10-25 DOI: 10.1016/j.tice.2024.102595

Yu-Xuan Huang , Yen-Chung Lin , Chin-Kai Lin , Haw-Ming Huang

{"title":"ω-9 monounsaturated fatty acids in Sapindus mukorossi seed oil enhance calcium deposition expression of Wharton’s jelly mesenchymal stem cells","authors":"Yu-Xuan Huang , Yen-Chung Lin , Chin-Kai Lin , Haw-Ming Huang","doi":"10.1016/j.tice.2024.102595","DOIUrl":"10.1016/j.tice.2024.102595","url":null,"abstract":"<div><div>Promoting osteogenesis is crucial to improve successful bone regeneration in bone tissue engineering. Several studies on osteogenesis have reported positive effects of ω-9 monounsaturated fatty acids (MUFA) on bone regeneration. This study examined the potential of ω-9 monounsaturated fatty acid abundant seed oil mechanically extracted from <em>Sapindus mukorossi</em> (<em>S. mukoro</em>ssi) fruit to improve osteogeneses. After showing the presence of ω-9 MUFA in <em>S. mukorossi</em> seed oil (SM oil) through GC-MS spectrum analysis, the proliferation and differentiation of human umbilical cord Wharton’s jelly mesenchymal stem cells (WJMSCs) under SM treatment was evaluated. Our results indicate that WJMSC differentiation was induced by adding an osteogenesis-induced medium combined with SM oil. A high level of calcium deposition expression in WJMSCs induced by SM oil appears to be due to the effect of oleic acid and eicosenoic acid, both of which are ω-9 monounsaturated fatty acids. In addition, we found major contributors of SM oil-promoted WJMSC osteogenesis to be extracellular signal-regulated kinase (ERKs), c-Jun N-terminal kinase (JNKs), and the p38 MAPK pathway. The enhancement of WJMSC osteogenesis via ERK/MAPK pathways as demonstrated by qPCR analysis indicates the promise of SM oil for stem cell-based bone tissue engineering applications.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142554232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mesenchymal stem cells alleviate autoimmune thyroiditis by modulating macrophage phenotypes and through influencing the STING pathway 间充质干细胞通过调节巨噬细胞表型和影响STING通路缓解自身免疫性甲状腺炎

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-10-25 DOI: 10.1016/j.tice.2024.102596

Haoran Ding , Jiabo Qin , Zhijian Liu , Xianbiao Shi , Wenxian Guan , Jianfeng Sang

{"title":"Mesenchymal stem cells alleviate autoimmune thyroiditis by modulating macrophage phenotypes and through influencing the STING pathway","authors":"Haoran Ding , Jiabo Qin , Zhijian Liu , Xianbiao Shi , Wenxian Guan , Jianfeng Sang","doi":"10.1016/j.tice.2024.102596","DOIUrl":"10.1016/j.tice.2024.102596","url":null,"abstract":"<div><h3>Background</h3><div>Hashimoto's thyroiditis is a chronic autoimmune inflammatory disease with a high prevalence and currently lacks effective treatment options. Previous preclinical and clinical trials have established mesenchymal stem cells (MSCs) as a promising therapeutic approach; however, there is limited research on MSC treatment for Hashimoto's thyroiditis, and the underlying molecular mechanisms remain unclear.</div></div><div><h3>Methods</h3><div>MSCs isolated from 4 to 6-week-old Lewis rats were employed for thyroiditis treatment. The efficacy of MSCs was assessed through histological and serological parameters. Molecular mechanisms of MSC therapy for Hashimoto's thyroiditis were explored by examining macrophage presence within thyroid tissue and relevant pathways.</div></div><div><h3>Results</h3><div>In this study, we observed elevated oxidative stress and endoplasmic reticulum stress within the thyroid tissue of Hashimoto's thyroiditis patients, and MSC therapy effectively mitigated this process. Furthermore, we found that the therapeutic potential of MSCs in the EAT model depended on the STING pathway. MSCs reduced endoplasmic reticulum stress and inflammasome levels within the thyroid tissue by modulating the STING pathway. Additionally, MSCs inhibited the expression of IRE1α in thyroid tissue macrophages, thereby reducing the polarization of M1-type macrophages</div></div><div><h3>Conclusions</h3><div>The STING pathway appears to be a crucial mechanism by which MSCs modulate macrophage polarization in thyroid tissue, offering a potential treatment for thyroiditis.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142533079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coloprotective effects of chebulic myrobalan extract by regulation of AMPK-SIRT1 signaling: A pharmacological and histopathological evaluation 诃子肉苁蓉提取物通过调节 AMPK-SIRT1 信号发挥结肠保护作用：药理学和组织病理学评估

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-10-23 DOI: 10.1016/j.tice.2024.102592

Mandeep Kaur , Debanjan Chatterjee , Shivani Singla , Inder Pal Singh , Gopabandhu Jena

{"title":"Coloprotective effects of chebulic myrobalan extract by regulation of AMPK-SIRT1 signaling: A pharmacological and histopathological evaluation","authors":"Mandeep Kaur , Debanjan Chatterjee , Shivani Singla , Inder Pal Singh , Gopabandhu Jena","doi":"10.1016/j.tice.2024.102592","DOIUrl":"10.1016/j.tice.2024.102592","url":null,"abstract":"<div><div>Ulcerative colitis is a chronic, refractory disease caused by dysregulation of mucosal immune responses to the indigenous bacterial flora as well as genetic and environmental variables. Recently, there has been increasing interest towards the use of herbal medicines for the treatment of ulcerative colitis and the potential benefits could lie in their high patient acceptability, effectiveness, safety, and relatively low cost. It has been reported that Chebulic myrobalan (Terminalia chebula) exhibits anti-oxidant, anti-inflammatory and immunomodulatory properties. The present study was designed to evaluate the protective potential of extract of dried fruit pulp of T. chebula against Dextran sulphate sodium (DSS)-induced ulcerative colitis in male BALB/c mice. Three cycles of DSS (3 % w/v in drinking water), each followed by a seven-day remission phase were used to induce ulcerative colitis in mice. Animals were treated with T. chebula (300 mg/kg and 600 mg/kg) starting from I<sup>st</sup> remission period to the end of the study. Different biochemical assays, histological evaluation and molecular analysis were performed to evaluate the protective effects of <em>T. chebula</em> extract in DSS induced colitis. <em>T. chebula</em> modulates the expression of nuclear factor kappa B, adenosine monophosphate kinase, tumour necrosis factor-alpha, sirtuin 1 and interleukin-1β. Furthermore, it also accorded coloprotective effects against DNA damage, apoptosis, inflammation and nitrosative stress. Finally, it was found that the high dose of the <em>T. chebula</em> extract (600 mg/kg) was found to be more effective than a low dose (300 mg/kg) in restoring the ulcerative colitis induced colonic damage.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142533077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hepatoprotective effect of silymarin-chitosan nanocomposite against aluminum-induced oxidative stress, inflammation, and apoptosis 水飞蓟素-壳聚糖纳米复合材料对铝诱导的氧化应激、炎症和细胞凋亡的肝保护作用

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-10-22 DOI: 10.1016/j.tice.2024.102591

Fatma M. El-Demerdash , Manal M. Ahmed , Wenyi Kang , Tarek M. Mohamed , Aliaa M. Radwan

{"title":"Hepatoprotective effect of silymarin-chitosan nanocomposite against aluminum-induced oxidative stress, inflammation, and apoptosis","authors":"Fatma M. El-Demerdash , Manal M. Ahmed , Wenyi Kang , Tarek M. Mohamed , Aliaa M. Radwan","doi":"10.1016/j.tice.2024.102591","DOIUrl":"10.1016/j.tice.2024.102591","url":null,"abstract":"<div><div>Aluminum (Al) is abundant in the environment, and its toxicity is attributed to free radical formation and subsequent oxidative stress. While silymarin is a well-known antioxidant, its low water solubility and bioavailability limit its therapeutic effects. This study was designated to formulate silymarin chitosan nanoparticles (SM-CS-NPs) and evaluate its ameliorative effect against hepatotoxicity induced by aluminum chloride (AlCl<sub>3</sub>). SM-CS-NPs were prepared by ionotropic gelation method and characterized using different techniques. Rats were distributed into six groups (n=7/group), control, silymarin (SM; 15 mg/kg B.W), silymarin-chitosan nanoparticles (SM-CS-NPs; 15 mg/kg), aluminum chloride (AlCl<sub>3</sub>, 34 mg/kg), SM or SM-CS-NPs administrated orally one hour before the treatment with AlCl<sub>3</sub> for 30 days, respectively. Results showed that supplementation of SM-CS-NPs or SM solo improved the antioxidant state and reduced oxidative stress. On the other hand, the pretreatment with SM-CS-NPs or SM followed by AlCl<sub>3</sub> significantly restored liver functions (AST, ALT, ALP, LDH, total protein, albumin, globulin, and bilirubin) and modulated oxidative stress biomarkers (TBARS and H<sub>2</sub>O<sub>2</sub>), with improved cellular antioxidant defense (SOD, CAT, GPx, GR, GST, and GSH) and maintained normal liver histological structure compared to rats treated with AlCl<sub>3</sub> alone. Furthermore, they alleviated the inflammation and apoptosis by downregulating the expression level of COX-2, caspase-3, and TNFα. This ameliorative effect was stronger with silymarin nanoform than in bulk-state silymarin. According to the findings, silymarin preparation in nanoform boosts its ameliorative and protective effects against AlCl<sub>3</sub> hepatotoxicity.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142508693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human amniotic membrane hydrogel loaded with exosomes derived from human placental mesenchymal stem cells accelerate diabetic wound healing 人羊膜水凝胶负载有提取自人胎盘间充质干细胞的外泌体，可加速糖尿病伤口愈合。

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-10-21 DOI: 10.1016/j.tice.2024.102590

Leila Varyani , Niloofar Ahmadpanah , Rozhin Kasiri , Shadman Shahzamani , Simindokht Tomraee , Aref Jafari , Hosna Mirjalili , Nassim Seyedi Asl

{"title":"Human amniotic membrane hydrogel loaded with exosomes derived from human placental mesenchymal stem cells accelerate diabetic wound healing","authors":"Leila Varyani , Niloofar Ahmadpanah , Rozhin Kasiri , Shadman Shahzamani , Simindokht Tomraee , Aref Jafari , Hosna Mirjalili , Nassim Seyedi Asl","doi":"10.1016/j.tice.2024.102590","DOIUrl":"10.1016/j.tice.2024.102590","url":null,"abstract":"<div><div>Diabetic wound is one of the most common and costly complication in diabetic patients. Hence, numerous studies have been carried out to discover a suitable approach to enhance the process of wound healing. Biological hydrogels are commonly utilized for wound healing due to their suitable properties among different materials available. Herein we investigated whether human amniotic membrane hydrogel (hAMH) loaded with human placental mesenchymal stem cells (PlaMSCs)-derived exosomes could promote healing in diabetic rats. Sixty diabetic rats were randomly assigned into the control group, hAMH group, exosome group, and hAMH+Exosome group. According to the phases of wound healing, sampling was done on days 7, 14, and 21 for further assessments. Our findings showed a significant increase in wound contraction rate, new epidermal length, fibroblast and blood vessel count, collagen density, and the levels of antioxidative factors (GSH, SOD, and CAT) in the treatment groups compared to the control group, with more pronounced effects observed in the hAMH+Exosome group. Furthermore, the levels of bFGF and VEGF gene expression significantly increased in each treatment group when compared to the control group, with the highest levels observed in the hAMH+Exosome group. This occurred as the hAMH+Exosome group showed a greater decrease in neutrophil count, the expression of TNF-α and IL-1β genes, and the levels of an oxidative factor (MDA) compared to the other groups. In summary, the combination of hAMH and PlaMSCs-derived exosomes was determined to have a more significant effect on healing diabetic wounds.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142547648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0