Tissue & cell最新文献

{"title":"Inhibition of proliferation, migration and invasion of RM-1 cells by roemerine: Insights from in vitro and in vivo studies.","authors":"Juntai Xu, Hongbin Ma","doi":"10.1016/j.tice.2024.102693","DOIUrl":"https://doi.org/10.1016/j.tice.2024.102693","url":null,"abstract":"<p><p>Prostate cancer is the second leading cause of cancer-related among men globally, with a rising incidence rate, particularly in developing countries. This highlights the urgent need to identify novel therapeutic agents. Roemerine, a naturally abundant alkaloid, has demonstrated antibacterial and antitumor properties, suggesting its potential utility in prostate cancer treatment. This study evaluates the effects of roemerine on RM-1 prostate cancer cells using in vitro assays and an in vivo nude mouse subcutaneous xenograft tumor model. Through Cell Counting Kit-8(CCK8), MTT, Wound-Healing, and Transwell assays, we assessed roemerine's impact on RM-1 cell proliferation, migration, and invasion. Additionally, the antitumor efficacy of roemerine was evaluated in nude mice bearing subcutaneous RM-1 tumors. Our findings reveal that roemerine significantly inhibits RM-1 cell activity, migration, invasion, and tumor growth. These results highlight the potential of roemerine as a novel antitumor agent for prostate cancer, providing a foundation for further mechanistic and pharmacological investigations.</p>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"93 ","pages":"102693"},"PeriodicalIF":2.7,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"METTL3/IGF2BP1 promotes the development of triple-negative breast cancer by mediating m6A methylation modification of PRMT7.","authors":"Wanli Lu, Shenghu Yang","doi":"10.1016/j.tice.2024.102690","DOIUrl":"https://doi.org/10.1016/j.tice.2024.102690","url":null,"abstract":"<p><strong>Background: </strong>PRMT7 is upregulated in breast cancer and promotes tumor metastasis. Here we aimed to explore the function and mechanism of PRMT7 in triple-negative breast cancer (TNBC).</p><p><strong>Methods: </strong>The expression of PRMT7, METTL3 and IGF2BP1 was detected by immunohistochemistry (IHC), qRT-PCR and western blot. Cell viability and proliferation were measured using MTT and EdU assay. Flow cytometry and TUNEL assays were used to evaluate apoptosis. Invasion and migration were assessed by transwell and wound healing assays, respectively. Glucose consumption and lactate production were measured to assess glycolysis. In addition, the interaction between METTL3 and PRMT was verified by methylated RNA immunoprecipitation. The roles of METTL3 and PRMT in vivo were investigated through a xenograft model.</p><p><strong>Results: </strong>PRMT7 was upregulated in TNBC tissues and cells, and the knockdown of PRMT7 inhibited cell proliferation, invasion, migration and glycolysis, but induced apoptosis in TNBC cells. METTL3/IGF2BP1 enhanced PRMT7 expression by mediating the m6A methylation modification of PRMT7. Besides, METTL3 knockdown suppressed the progression of TNBC cells and regulated the WNT/β-catenin pathway via PRMT7. Moreover, silencing METTL3 restrained TNBC tumor growth in vivo through regulating PRMT7.</p><p><strong>Conclusion: </strong>METTL3/IGF2BP1 facilitates the progression of TNBC by mediating m6A methylation modification of PRMT7.</p>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"93 ","pages":"102690"},"PeriodicalIF":2.7,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of adult stem cells-derived mitochondria transfer combined with curcumin administration into ARPE-19 cells in age-related macular degeneration model.","authors":"Kamil Can Kılıç, Gökhan Duruksu, Ahmet Öztürk, Selenay Furat Rençber, Buket Kılıç, Yusufhan Yazır","doi":"10.1016/j.tice.2024.102687","DOIUrl":"https://doi.org/10.1016/j.tice.2024.102687","url":null,"abstract":"<p><strong>Objective: </strong>Mitochondria transfer from human Wharton's Jelly-derived mesenchymal stem cells (hWJ-MSCs-mt) and human endometrium-derived mesenchymal stem cells (hE-MSCs-mt), along with curcumin, were explored as potential treatments for age-related macular degeneration (AMD) caused by mitochondrial inefficiency, using a retinal model to assess impacts of curcumin and hWJ-MSCs-mt or hE-MSCs-mt on AMD.</p><p><strong>Methods: </strong>ARPE-19 cells established an in vitro AMD model. Cells were exposed to 0-50 μM curcumin for 24 hours to determine optimal concentration by assessing their viability. Immunofluorescence examined SOD1, TNF-α, and TGF-β levels at optimal hydrogen peroxide (H₂O₂) concentration. β-galactosidase staining and DCFH analysis evaluated H₂O₂-induced cellular senescence. Immunofluorescence assessed REP65, CRALBP1 (RLBP1), Pink1, and Parkin expression, whereas qRT-PCR analyzed Nrf2, Ire1a, ARMS2, HTRA1, RPE65, RLBP1, NOX4, and TOMM20 expression following co-treatment with curcumin and hWJ-MSCs-mt or hE-MSCs-mt.</p><p><strong>Results: </strong>Curcumin improved ARPE-19 cell survival under H₂O₂-induced oxidative stress by regulating SOD1, TNF-α, TGF-β, DCFH, and MDA levels. hWJ-MSCs-mt transfer increased RLBP1 and Parkin expression, whereas curcumin reduced Parkin expression. hE-MSCs-mt transfer upregulated Parkin, RPE65, Pink1, and RLBP1 expressions, with curcumin enhancing RPE65 expression. hWJ-MSCs-mt and curcumin combined more effectively downregulated expressions of stress-related genes (Nrf2, Ire1α, NOX4) and improved expression of mitochondrial function gene (TOMM20). hE-MSCs-mt transfer with curcumin synergistically enhanced expression of retinal health markers (RPE65, RLBP1) and downregulated expression of damage-associated genes (HTRA1, ARMS2) in AMD models.</p><p><strong>Conclusion: </strong>Curcumin combined with hWJ-MSCs-mt or hE-MSCs-mt is a potential AMD therapy owing to its anti-inflammatory properties; however, further in vivo and human studies are needed to confirm its efficacy and safety.</p>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"93 ","pages":"102687"},"PeriodicalIF":2.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gbp2 driving macrophages dynamics in murine heart transplant.","authors":"Baotong Zhang, Wenbin Ji, Duowei Wang, Guoshan Chen, Wenhao Xiong, Feng Qi","doi":"10.1016/j.tice.2024.102695","DOIUrl":"https://doi.org/10.1016/j.tice.2024.102695","url":null,"abstract":"<p><strong>Background and objective: </strong>Organ transplantation is a vital treatment for patients with end-stage organ diseases, and macrophages play a key role in the rejection process. This study seeks to pinpoint key genes responsible for the dynamic changes in macrophages during rejection and to evaluate their impact on macrophage polarization through bioinformatics analysis.</p><p><strong>Methods: </strong>We selected single-cell sequencing data of mouse heart transplant models from Genome Sequence Archive to construct a dynamic landscape of immune cells during acute rejection. Key genes involved in macrophage changes were screened using pseudotime analysis and hdWGCNA. The mouse heart transplant models also were established to validate changes of the key genes during rejection.</p><p><strong>Results: </strong>Bioinformatics analysis identified Gbp2 as the key gene driving macrophage dynamics during rejection, which was also confirmed in another dataset showed Gbp2 levels increased in macrophages during acute rejection. Further experiments validated the upregulation of Gbp2 in both tissues and macrophages during acute rejection, and in vitro experiments confirmed Gbp2 increasing in M1 macrophages.</p><p><strong>Conclusion: </strong>Gbp2 is a key gene that regulates macrophage polarization during acute rejection, making it a potential therapeutic target for the acute rejection.</p>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"93 ","pages":"102695"},"PeriodicalIF":2.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan hydrogel incorporated with bone marrow mesenchymal stem cell-derived exosomal TIMP2 to inhibit angiogenesis in cholangiocarcinoma.","authors":"Fei Song, Dan Xu, Jiayin Che, Ming Huang, Hongyang Li","doi":"10.1016/j.tice.2024.102694","DOIUrl":"https://doi.org/10.1016/j.tice.2024.102694","url":null,"abstract":"<p><strong>Objective: </strong>Cholangiocarcinoma (CCA) presents a therapeutic challenge due to its aggressiveness and poor survival rates. This study introduces an approach using tissue inhibitor of metalloproteinase 2 (TIMP2)-enriched bone marrow mesenchymal stem cell-derived exosomes (BMSC-Exo) encapsulated in chitosan hydrogels (CS), intending to provide novel insight into the CCA treatment.</p><p><strong>Methods: </strong>BMSC-Exo was characterized by using TEM, nanoparticle tracking analysis, and western blotting. Role of TIMP2 in CCA was explored using bioinformatics analysis. Therapeutic efficacy and mechanisms of BMSC-Exo/CS in CCA were assessed through cell viability tests and colony formation assays. Angiogenic and Wnt/β-catenin signaling pathways-related key factors were detected through RT-qPCR or western blotting.</p><p><strong>Results: </strong>BMSC-Exo displayed typical cup-shaped morphology and was positive for exosomal markers CD9 and TSG101, but negative for endoplasmic reticulum marker Calnexin, with a diameter of 124.6 nm. BMSC-Exo combined with CS showed synergistic anti-proliferative effects in CCA cells. High-expression TIMP2 samples indicated a better prognosis of CCA patients, and BMSC-Exo/CS increased the TIMP2 expression in CCA cells. Mechanistically, BMSC-Exo/CS TIMP2 overexpression inhibited key factors related to angiogenesis (VEGFA and VEGFR2) and Wnt/β-catenin pathway (β-catenin and c-Myc), thereby reducing CCA cell viability. Notably, these inhibitory effects were reversed by a Wnt signaling agonist (BML-284).</p><p><strong>Conclusion: </strong>The study validates the therapeutic potential of BMSC-Exo/CS TIMP2 in CCA treatment. This innovative approach targets angiogenesis and Wnt/β-catenin signaling, providing a new avenue for more effective and comprehensive CCA therapies.</p>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"93 ","pages":"102694"},"PeriodicalIF":2.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fibrin scaffold encapsulated with epigallocatechin gallate microspheres promote neural regeneration and motor function recovery after traumatic spinal cord injury in rats.","authors":"Mohammed Alissa, Abdullah Alghamdi, Mohammed A Alshehri","doi":"10.1016/j.tice.2024.102691","DOIUrl":"https://doi.org/10.1016/j.tice.2024.102691","url":null,"abstract":"<p><p>Traumatic spinal cord injury (TSCI) is a serious medical issue where there is a loss of sensorimotor function. Current interventions continue to lack the ability to successfully enhance these conditions, therefore, it is crucial to consider alternative effective strategies. Currently, we investigated the effects of fibrin scaffold encapsulated with epigallocatechin gallate (EGCG) microspheres in the recovery of SCI in rats. A total of sixty mature male Sprague-Dawley rats were separated into four groups of the same size: TSCI, fibrin, EGCG, and Fibrin+EGCG. Samples of tissue were gathered at the location of the injury for additional examination. The treatment groups showed significantly higher levels of neurons, antioxidative biomarkers (T-AOC: total antioxidant capacity, GSH: glutathione, and SOD: superoxide dismutase), neurofilament light polypeptide (NEFL) and interleukin 10 (IL-10) genes, and neurological function scores compared to the TSCI group, with the Fibrin+EGCG group displaying the most noticeable improvements. Throughout the treatment process, there was a notable reduction in the amounts of apoptotic and glial cells, as well as levels of malondialdehyde (MDA) and proinflammatory genes (TNF-α: tumor necrosis factor alpha and IL-1β: interleukin-1 beta), especially in the Fibrin+EGCG group compared to the TSCI group. Our findings suggest that EGCG enclosed in microspheres could enhance the prevention of injury spreading and the enhancement of pathological and behavioral symptoms when delivered to the location of spinal cord injury using a fibrin scaffold.</p>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"93 ","pages":"102691"},"PeriodicalIF":2.7,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxymatrine ameliorates epithelial mesenchymal transition in IgA nephropathy induced rats.","authors":"Rajiv Jash, Himangshu Sekhar Maji, Arnab Chowdhury, Kousik Maparu, Sanket Seksaria, Priyanka Gupta, Arghya Paria, Arijit Nandi, Anwesha Das, Bornika Chattaraj, Ds Nb K Prasanth","doi":"10.1016/j.tice.2024.102671","DOIUrl":"https://doi.org/10.1016/j.tice.2024.102671","url":null,"abstract":"<p><p>In this study, we investigated the efficacy of oxymatrine, a phytochemical alkaloid, in reducing inflammation and fibrosis in a rat model of IgA nephropathy (IgAN) through modulation of the TGF-β/SMAD signaling pathway. Thirty Sprague Dawley rats were randomized into control, IgAN, and treatment groups, the latter receiving oxymatrine postinduction of IgAN. Induced by bovine serum albumin, carbon tetrachloride, and lipopolysaccharides, the disease model was validated by immunofluorescence and histopathological analyses, confirming significant renal deposition of IgA and increased fibrosis markers (IL-6, TGF-β, SMAD 3, and α-SMA). Oxymatrine treatment led to a notable decrease in urine protein levels and red blood cells at 10 weeks, suggesting reduced kidney damage. There was no significant impact on the SGOT or SGPT levels, while it reduced the BUN, serum ALB and creatinine levels, indicating minimal hepatotoxicity and renoprotective effects. Histopathology demonstrated preservation of the glomerular diameter in the treatment group. Immunofluorescence and ELISA revealed a reduction in the levels of proinflammatory (IL-6,TNF-α) and profibrotic (TGF-β, SMAD 3, and α-SMA) markers in treated rats, suggesting that oxymatrine has renoprotective effects on the inhibition of pathological EMT processes and fibrosis in IgAN. Our results suggest that oxymatrine is a potential therapeutic agent for IgAN that attenuates disease progression by targeting the TGF-β/SMAD pathways involved in EMT and fibrosis. Further research is warranted to establish long-term efficacy and safety profiles.</p>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"93 ","pages":"102671"},"PeriodicalIF":2.7,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphology of the head-associated exocrine glands in Cornitermes cumulans with the description of a novel gland for the worker caste.","authors":"Ana Maria Costa-Leonardo, Vanelize Janei, Lorena Maria Gardesani Bacci, Iago Bueno da Silva","doi":"10.1016/j.tice.2024.102688","DOIUrl":"https://doi.org/10.1016/j.tice.2024.102688","url":null,"abstract":"<p><p>Exocrine glands are important mediators of communication in eusocial insects and the description of novel glands reflects the complex context in which these animals live. Here we revisit the head-associated glands in workers of the Neotropical termite Cornitermes cumulans through histological analysis and describe a novel gland for this caste, the intramandibular glands. This structure is located underneath the cuticle of the dorsodistal part of each mandible. The glands showed an epithelial arrangement, but the cytological morphology is complex, comprising classes I and III of secretory cells. The present data highlight the importance of the intramandibular glands in the worker caste and demonstrate different morphology of these glands in Isoptera, probably related to the specialized function of the castes. Features of active glandular activity were also observed in the mandibular, labral, and salivary glands of these workers. Despite the intramandibular glands being also found in workers of other social insects, their occurrence in termites was restricted to the soldier caste of Machadotermes. Even so, their cytological structure differs from those of C. cumulans workers. The likely function of the worker intramandibular glands is discussed considering the separate-nest life type present by C. cumulans and tasks performed by workers. The chemical nature of the secretion and the occurrence of the glands in other termite taxa still require further investigation.</p>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"93 ","pages":"102688"},"PeriodicalIF":2.7,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decorin alleviates non-alcoholic fatty liver disease in rats with polycystic ovary syndrome.","authors":"Hany A Elkattawy, Amira Ebrahim Alsemeh, Lashin Saad Ali, Mona Mostafa Ahmed, Asmaa Monir Eltaweel, Farha M Shaikh, Ahmed Behiry, Ahmed El-Sayed Hassan, Deema Kamal Sabir, Dalia Mahmoud Abdelmonem Elsherbini, Sahar K Ali, Madaniah Omar Zakari, Moaz Abdullah Mojaddidi, Ehab Kamal Ali, Yasser M Elbastawisy, Shimaa Hadhoud","doi":"10.1016/j.tice.2024.102689","DOIUrl":"https://doi.org/10.1016/j.tice.2024.102689","url":null,"abstract":"<p><p>Endocrine multisystem defect polycystic ovary syndrome (PCOS) causes hyperandrogenism and infertility. Half of PCOS women have (non-alcoholic fatty liver disease) NAFLD, which increases metabolic disease risk. We tested decorin's effect on NAFLD and related processes in PCOS. NAFLD+PCOS, PCOS+decorin, and control rats were studied. Decorin was evaluated on NAFLD/PCOS rats. Test group rats received HF for eight weeks to generate NAFLD. The rats got 1 mg/kg letrozole orally daily for 21 days to diagnosis PCOS. Afterward, rats got injectable decorin for 14 days. Body weight, liver weight, liver coefficient Abdominal Circumference (AC) and body mass index (BMI) were determined. Blood triglycerides (TG), total cholesterol, LDL-c, AST, and glucose were measured. The insulin, testosterone, estrogen, LH, and FSH were measured by ELISA. GPx, SOD, MDA, TNF-, and Caspase-3 liver immunohistochemistry were evaluated. NAFLD liver tissues in PCOS models showed biochemical and histological alterations. NAFLD+PCOS raised BMI, AC, liver weight, and coefficient. Blood glucose, insulin resistance, TG, ALT, and AST increased. Lipid abnormalities (TG, cholesterol, LDL-c, and HDL-c), oxidative stress markers (MDA, SOD, and GPx), and liver dysfunction were found. Low serum E2 and high T supported PCO. Decorin reduced model rat BMI, liver weight, coefficient, insulin resistance, TG, ALT, and AST. It reduced liver inflammation, improved liver extract lipids, and normalized MDA, SOD, and GPx. In the model group, decorin lowered serum T, E2, LH, caspase 3, and TNF-alpha. Decorating improved NAFLD/PCOS group liver histology and function. Decorin reduces hepatosteatosis by reducing liver inflammation, oxidative stress, and dyslipidemia.</p>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"93 ","pages":"102689"},"PeriodicalIF":2.7,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myricetin alleviates the mechanism of IL-1β production caused by the endocrine-disrupting chemical Di(2-ethylhexyl) phthalate in RAW 264.7 cells.","authors":"Ji-Yeon Park, Sei-Jung Lee","doi":"10.1016/j.tice.2024.102683","DOIUrl":"https://doi.org/10.1016/j.tice.2024.102683","url":null,"abstract":"<p><p>Myricetin, a flavonoid present in numerous fruits, vegetables, and medicinal plants, is recognized for its potent antioxidant, anti-inflammatory, and anti-cancer activities. Nevertheless, its involvement in mitigating inflammation caused by the endocrine-disrupting chemical Di(2-ethylhexyl) phthalate (DEHP), commonly used in polyvinyl chloride (PVC) manufacturing to improve flexibility, has not been investigated. Here, we found that DEHP markedly increased IL-1β production through inflammatory pathways in RAW 264.7 murine macrophages. Treatment with myricetin at a concentration of 10 μM significantly reduced the elevated IL-1β levels. Myricetin achieves this by inhibiting the activation of protein kinase C (PKC) and extracellular signal-regulated kinase (ERK), which are driven by reactive oxygen species (ROS), thereby suppressing IL-1β transcription via nuclear factor-kappa B (NF-κB). Additionally, myricetin prevents ROS-induced activation of the NLRP3 inflammasome and subsequent caspase-1 activation, further decreasing IL-1β production. These dual actions highlight myricetin's therapeutic potential in countering the oxidative stress-mediated inflammatory pathways triggered by environmental toxins like DEHP.</p>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"93 ","pages":"102683"},"PeriodicalIF":2.7,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142830006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}