Tissue & cell最新文献

{"title":"Exercise ameliorates cardiac injury induced by nandrolone decanoate through downregulation of osteopontin and mTOR expressions.","authors":"Mohamed Aref, Wesam Mr Ashour, Nanees F El-Malkey, Haifa A Alqahtani, Mohamed A Nassan, Noha Ali Abd-Almotaleb, Gamal A Salem","doi":"10.1016/j.tice.2025.102932","DOIUrl":"https://doi.org/10.1016/j.tice.2025.102932","url":null,"abstract":"<p><p>Nandrolone-decanoate (NA), a synthetic anabolic steroid, negatively impacts cardiac function. While exercise is known to benefit cardiovascular health, its effects on individuals misusing anabolic steroids require further study. Osteopontin (OPN) and mammalian target of rapamycin (m-TOR) are crucial in inflammation-related cardiovascular diseases and can be influenced by exercise, though results are inconclusive. This study aims to examine how exercise affects NA's cardiac adverse effects and the potential role of OPN and m-TOR. The study involved 52 male rats divided into four groups: control, exercise-only, NA-treated (15 mg/kg/day S.C for 8 W), and combined exercise and NA treatment. Researchers measured blood pressure, heart rate (HR), serum cardiac enzymes, CRP, IL-1B, IL-6, Brain Natriuretic Peptide (BNP) and conducted macro and micromorphological assessments. Additionally, immunohistochemical analysis of cardiac OPN and mTOR was performed. The NA-treated group showed significant increases in blood pressure, HR, weight, and cardiac enzymes compared to the control group. Exercise significantly improved these parameters in the combined exercise and NA treatment group, except for blood pressure. All groups exhibited an increase in cardiac weight relative to the control. The NA-treated group displayed marked hyaline degeneration and necrosis in cardiac tissues, with increased cell diameter and excess collagen deposition, which was less severe in the combined exercise (EX) and NA treatment group. NA treatment significantly elevated inflammatory mediators and the area percentage of OPN and m-TOR expression. These markers were significantly reduced in the combined exercise and NA treatment group. BNP was remarkably raised in EX+NA group compared to all other groups. Exercise mitigated NA-induced cardiac damage by reducing inflammation, possibly through the downregulation of cardiac OPN and m-TOR expression.</p>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"95 ","pages":"102932"},"PeriodicalIF":2.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of type 1 diabetes mellitus on male fertility and embryo quality in superovulated mice.","authors":"Begum Alyürük, Yusufhan Yazir, Zeynep Ece Utkan Korun, Özcan Budak, Ender Yalçinkaya Kalyan, Kamil Can Kiliç","doi":"10.1016/j.tice.2025.102941","DOIUrl":"https://doi.org/10.1016/j.tice.2025.102941","url":null,"abstract":"<p><strong>Objective: </strong>We aimed to compare embryo quality, sperm morphology, motility, and fertilization obtained from male mice with type 1 diabetes mellitus (T1DM) induced by streptozotocin (STZ) in control and diabetic mice undergoing in vitro fertilization (IVF).</p><p><strong>Methods: </strong>CD-1 male mice were divided into control and DM groups, with an i.p. injection of 100 mg/kg STZ to induce T1DM. One month later, the mice were euthanized to investigate the effects of STZ-induced T1DM on the reproductive system. Sperms were obtained from the epididymis and vas deferens. The morphology and motility of the cells were evaluated. Follicle development was stimulated by controlled ovarian stimulation, and oocytes were collected by extracting oviducts and ovaries from female mice housed under controlled environmental conditions with ad libitum access. Both groups underwent IVF with fertilized zygotes followed up until the third day before embryo quality was compared.</p><p><strong>Results: </strong>Female mice bred with diabetic males exhibited significantly lower fertilization rates than the controls (p < 0.05). Sperm from diabetic mice displayed abnormalities in shape and movement, with reduced motility and fertilization. Embryos from male diabetic mice exhibited a higher incidence of developmental arrest during early embryogenesis. Although no significant differences in oocyte quality were observed, embryos from diabetic mice exhibited higher growth rates. These findings highlighted the T1DM's detrimental effects on sperm morphology, motility, fertilization, and early embryonic development, thus contributing to our understanding of reproductive complications.</p><p><strong>Conclusion: </strong>In conclusion, our findings demonstrated that T1DM significantly impaired sperm morphology, motility, and fertilization capacity, leading to reduced embryo quality and increased developmental arrest. These results highlight the detrimental impact of DM on male reproductive potential and underscore the importance of glycemic control in optimizing outcomes in assisted reproductive techniques such as IVF.</p>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"95 ","pages":"102941"},"PeriodicalIF":2.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative therapeutic strategies used in the regenerative medicine of the intervertebral disc degeneration","authors":"Najah Elmounedi ,&nbsp;Hassib Keskes","doi":"10.1016/j.tice.2025.103069","DOIUrl":"10.1016/j.tice.2025.103069","url":null,"abstract":"<div><div>Low back pain (LBP) is one of the top disorders that affects patients’ quality of life and imposes a heavy socio-economic burden. It is estimated that over 80 % of the global population will experience LBP at least once in their lifetime. Approximately 40 % of these cases are linked to intervertebral disc (IVD) degeneration. Current treatments are predominantly symptomatic, focusing on pain relief rather than addressing the root cause of intervertebral disc degeneration (IDD). However, recent advances in our understanding of IDD pathophysiology have paved the way for novel, etiological therapeutic strategies, particularly regenerative medicine approaches targeting the IVD. This review first outlines the key physiopathological mechanisms involved in IDD. It then examines emerging therapeutic strategies, including exogenous repair through cell therapy and tissue engineering, as well as endogenous repair via molecular biotherapy and gene therapy, emphasizing their potential and applicability in combating disc degeneration.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"97 ","pages":"Article 103069"},"PeriodicalIF":2.5,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144767228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flavonoid-rich Deverra tortuosa extract mitigates ethanol-induced gastritis via modulation of cytokine networks, oxidative pathways, and mucosal immunotoxicity","authors":"Mohamed F. Abdelhameed ,&nbsp;Mosab Gad ,&nbsp;Heba A.S. El-Nashar ,&nbsp;Rehab F. Taher ,&nbsp;Rehab F. Abdel-Rahman ,&nbsp;Asmaa S. Abd Elkarim ,&nbsp;Alaa M. Ali ,&nbsp;Mohamed A. Farag ,&nbsp;Abdelsamed I. Elshamy","doi":"10.1016/j.tice.2025.103067","DOIUrl":"10.1016/j.tice.2025.103067","url":null,"abstract":"<div><div>Gastric ulcers are a major gastrointestinal disorder influenced by environmental and lifestyle factors, warranting for the discovery of effective therapeutic alternatives. <em>Deverra tortuosa</em> (Desf.) DC., a traditional medicinal plant, has demonstrated potential gastroprotective properties. This study assesses the anti-ulcerative effects of phenolic-rich ethanolic extract of <em>D. tortuosa</em> (DT-ETOH) against ethanol-induced gastritis in rats, alongside its underlying mechanisms of action. Comprehensive metabolites profiling via UHPLC-ESI-qTOF-MS/MS identified 35 bioactive compounds, primarily flavonoids and phenolic acids, along with coumarins, sterols, and fatty acids. Oral administration of DT-ETOH significantly mitigated gastric ulceration by reducing the ulcer index and oxidative stress markers, enhancing antioxidant enzyme activity (SOD, GSH), and modulating key inflammatory mediators (TNF-α, IL-6). Notably, DT-ETOH administration led to the upregulation of Keap-1 and HO-1 expression, increased iKba levels, and suppression of iNOS, indicating its role in oxidative stress regulation and inflammation suppression. Histopathological findings further confirmed the protective effects of DT-ETOH, demonstrating improved gastric mucosal integrity. These results provide evidence of DT-ETOH's gastroprotective, antioxidant, and anti-inflammatory properties, supporting its potential as a natural agent for gastric ulcer management.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"97 ","pages":"Article 103067"},"PeriodicalIF":2.5,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144757667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alterations of matrisome gene expression in multipotent mesenchymal stromal cells under physiological hypoxia in vitro","authors":"Diana Matveeva ,&nbsp;Elena Andreeva,&nbsp;Yulia Rudimova,&nbsp;Aleksandra Gornostaeva,&nbsp;Danila Yakubets,&nbsp;Irina Andrianova,&nbsp;Ludmila Buravkova","doi":"10.1016/j.tice.2025.103064","DOIUrl":"10.1016/j.tice.2025.103064","url":null,"abstract":"<div><div>Low O₂ level (physiological hypoxia) is an important physical parameter in local tissue niches of multipotent mesenchymal stromal cells (MSCs). Hypoxia preconditioning is actively applied in cell therapy and regenerative medicine protocols. In the present study, the effect of physiologic hypoxia <em>in vitro</em> (5 % O₂) on the extracellular matrix of MSCs from the stromal-vascular fraction of human adipose tissue was investigated. Compared to standard cell culture conditions (20 % O₂), the genes encoding structural and regulatory proteins of the extracellular matrix (matrisome) were differentially expressed in MSCs under physiologic hypoxia. There was a significant downregulation of genes coding structural glycoproteins (<em>COMP, ELN</em>) in the core matrisome and upregulation of genes encoded matrisome-associated proteins with pro-migratory (<em>CXCL12</em>) and antioxidant (<em>SRPX, SERPINF1</em>) functions. At different O₂ levels, there were no significant differences in immunocytochemically identified the core matrisome proteins: collagen I, fibronectin, osteonectin, versican, nor in the expression of the corresponding genes. Meanwhile, variations in packaging patterns of the fibrils were, however, demonstrated using scanning electron microscopy. Under 5 % O₂ the activities of the soluble matrix metalloproteinases MMP-1 and MMP-2 were reduced. Thus, physiological hypoxia modulates the matrisome properties, and understanding this may be important for gaining mechanistic insights into the activity of MSCs in local tissue microenvironments, as well as in the protocols for scaffold production based on native ECMs for use in regenerative medicine.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"97 ","pages":"Article 103064"},"PeriodicalIF":2.5,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144757520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"B7-H3 enhances the malignant phenotype of colorectal cancer by activating the Wnt/β-catenin pathway mediated by CYP1B1","authors":"Xingxiang Liu ,&nbsp;Jie Ding ,&nbsp;Qiumei Zong ,&nbsp;Yong Mao","doi":"10.1016/j.tice.2025.103066","DOIUrl":"10.1016/j.tice.2025.103066","url":null,"abstract":"<div><h3>Background</h3><div>Colorectal cancer (CRC) is a prevalent malignancy. Previous studies have identified aberrant expression of Cytochrome P450 1B1 (CYP1B1) in human CRC tissues; however, the exact role and molecular mechanism of CYP1B1 in CRC remain unknown.</div></div><div><h3>Methods</h3><div>This study initially assessed the expression of CYP1B1 in human colorectal cancer samples and cell lines. Subsequently, siRNA or overexpression plasmid of CYP1B1 was transfected into CaCo-2 or SW480 cells, and cell proliferation, cycle, migration, and invasion were analyzed using CCK8, Colony-formation, Ethynyl-20-deoxyuridine incorporation, flow cytometry, wound healing and transwell assays, and the expression level of CYP1B1 was determined by quantitative real-time polymerase chain reaction and western blotting.</div></div><div><h3>Results</h3><div>The results indicated that up-regulation of CYP1B1 in CRC samples compared to normal tissues, with its expression level correlating with the local infiltration status. CYP1B1 influenced the proliferation of CRC cells, induced epithelial-mesenchymal transition through the Wnt/β-catenin pathway, thereby promoting cell migration and invasion. Additionally, CYP1B1 expression could be regulated by B7-H3 through the PI3K-AKT pathway.</div></div><div><h3>Conclusions</h3><div>Those findings suggested that overexpression of CYP1B1 enhances the migration and invasion of human CRC cells by activating the PI3K/Akt signaling pathway through B7-H3.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"97 ","pages":"Article 103066"},"PeriodicalIF":2.5,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144781133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The possible role of lycopene on the cerebellum of albino rat prenatally exposed to valproic acid: Animal model of autism spectrum disorder","authors":"Lubna T. Eid ,&nbsp;Mostafa M. El-Habeby ,&nbsp;Noha M. Issa ,&nbsp;Nermeen M. Nooreldien","doi":"10.1016/j.tice.2025.103062","DOIUrl":"10.1016/j.tice.2025.103062","url":null,"abstract":"<div><div>Autism is a serious neurodevelopmental disorder with a rising global prevalence. Prenatal exposure to valproic acid (VPA), a common antiepileptic drug, is associated with autism in offspring. Lycopene, a potent antioxidant and anti-inflammatory compound, may counteract VPA-induced neurotoxicity. To the best of our knowledge, this research is the first attempt to assess the beneficial role of lycopene supplementation in a VPA model of autism. In total, 30 pregnant female albino rats were grouped into five groups: control, lycopene-treated (5 mg/kg/day orally), VPA-treated (50 mg/kg/day orally), VPA-protected with lycopene, and VPA-treated with lycopene. After the scarification of rats, biochemical, histological, immunohistochemical, and ultrastructural analyses were performed on the cerebellum. VPA produced degenerative changes in the cerebellum with increased glial fibrillary acidic protein (GFAP) and Bax while decreasing myelin basic protein (MBP) and Tau1 expressions. Moreover, it increased the brain levels of malondialdehyde (MDA), acetylcholinesterase enzyme (AChE), tumor necrosis factor-alpha (TNF-α), and glutamate. It also reduced brain-derived neurotrophic factor (BDNF) and superoxide dismutase (SOD) levels. Lycopene reversed these effects by reducing oxidative stress and inflammatory markers, and restoring antioxidant levels. In conclusion, lycopene mitigated VPA-induced cerebellar damage through its antioxidant and anti-inflammatory effects and its ability to modulate neurotransmission, suggesting a potential therapeutic role in autism.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"97 ","pages":"Article 103062"},"PeriodicalIF":2.5,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144772672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A collagen-based amniotic membrane scaffold combined with photobiomodulation accelerates wound repair in diabetic rats through modulation of inflammation and tissue regeneration","authors":"Ahmed Hjazi","doi":"10.1016/j.tice.2025.103063","DOIUrl":"10.1016/j.tice.2025.103063","url":null,"abstract":"<div><div>Chronic diabetic wounds present significant challenges to effective tissue repair due to persistent inflammation and impaired regeneration. In this study, we investigated the therapeutic potential of a bioengineered collagen scaffold derived from the human amniotic membrane (CSAM), used alone or in combination with photobiomodulation therapy (PBMT), in a diabetic rat wound model. Forty diabetic rats were allocated into four groups: control, CSAM, PBMT, and CSAM+PBMT. Wound healing was assessed on days 4 and 8 post-injury. Combined treatment (CSAM+PBMT) significantly enhanced wound closure and histological regeneration, with notable increases in fibroblast and blood vessel density, epidermal and dermal thickness, collagen deposition, and expression levels of VEGF and bFGF compared to other groups. Additionally, this group exhibited a marked reduction in neutrophil infiltration and pro-inflammatory cytokines, including IL-1β, TNF-α, and NF-κB. These results demonstrate that the synergistic application of PBMT and CSAM fosters a pro-regenerative wound microenvironment by suppressing inflammation and promoting cellular proliferation and extracellular matrix remodeling. This combinatory approach offers a promising therapeutic avenue for improving diabetic wound healing outcomes.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"97 ","pages":"Article 103063"},"PeriodicalIF":2.5,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144739533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A combined therapeutic approach: Parthenolide and vincristine modulate autophagy in B-cell acute lymphoblastic leukemia","authors":"Elmira Zarei ,&nbsp;Sepide Namdari ,&nbsp;Farahnaz Zare ,&nbsp;Gholamreza Rafiei Dehbidi ,&nbsp;Mansoureh Nazari ,&nbsp;Gholamhossein Tamaddon ,&nbsp;Azadeh Omidkhoda","doi":"10.1016/j.tice.2025.103057","DOIUrl":"10.1016/j.tice.2025.103057","url":null,"abstract":"<div><h3>Background</h3><div>Parthenolide (PTL), derived from <em>Tanacetum parthenium</em>, is known for its anti-inflammatory and anti-cancer properties, primarily through NF-κB inhibition and modulation of reactive oxygen species. This study investigates PTL's potential as a complementary treatment for B-Cell Acute Lymphoblastic Leukemia, focusing on its effects on autophagy, apoptosis, and proliferation in the Nalm-6 cell line, alongside vincristine, with the aim of addressing challenges such as multi-drug resistance.</div></div><div><h3>Methods</h3><div>Nalm-6 cells were treated with increasing concentrations of PTL and VCR to determine their IC50 values, followed by combination treatments with sub-IC50 doses. Apoptosis was assessed using flow cytometry, while changes in autophagy-related gene expression (BECN1, ATG10, LC3, and P62) were measured via Real-time PCR, and LC3 protein levels were analyzed through Immunoblotting.</div></div><div><h3>Results</h3><div>The results revealed that both PTL and VCR inhibited Nalm-6 cell proliferation in a concentration and time-dependent manner, with a synergistic effect observed in combined treatments. This combination also increased apoptosis and significantly enhanced the expression of autophagy-related genes ATG10, LC3 and BECN1 while decreasing P62 expression. Additionally, VCR and PTL increased LC3B-II protein levels.</div></div><div><h3>Conclusion</h3><div>Overall, the study indicates that combined treatment promotes autophagy and apoptosis, suggesting that PTL could be a beneficial adjunct to chemotherapy for treating B-ALL in the future.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"97 ","pages":"Article 103057"},"PeriodicalIF":2.5,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144739531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ELAVL1 promotes ferroptosis-induced inhibition of osteogenic differentiation in diabetic osteoporosis by downregulating SIRT1","authors":"Bi Huang ,&nbsp;Jie Jiang ,&nbsp;Xiang Ou ,&nbsp;Meng Hao ,&nbsp;Huige Shao","doi":"10.1016/j.tice.2025.103060","DOIUrl":"10.1016/j.tice.2025.103060","url":null,"abstract":"<div><h3>Objective</h3><div>Ferroptosis can implicate in the pathogenesis of diabetic osteoporosis (DOP). This study aimed to determine whether Sirtuin 1 (SIRT1) modulates DOP through ELAV-like RNA binding protein 1 (ELAVL1)-mediated ferroptosis.</div></div><div><h3>Methods</h3><div>MC3T3-E1 cells were cultured in high glucose (HG) medium, and osteogenic differentiation was assessed by evaluating RUNX2, OCN, ALP activity, and calcium deposition via Alizarin Red S staining. Ferroptosis was evaluated by measuring GPX4, ACSL4, ROS, MDA, and Fe^2 + . A DOP mouse model was established using C57BL/6 J mice fed a high-fat diet combined with 1 % streptozotocin injection. Femoral histopathology, body weight, fasting blood glucose (FBG), and ferroptosis were analyzed.</div></div><div><h3>Results</h3><div>HG exposure inhibited osteogenic differentiation, characterized by the reduction in RUNX2, OCN ALP activity and calcium accumulation in MC3T3-E1 cells (<em>P</em> &lt; 0.05); it induced ferroptosis, including the decrease in GPX4 and the increase in ACSL4, ROS, MDA and Fe²⁺ (<em>P</em> &lt; 0.05). SIRT1 overexpression or treatment with Fer-1 reversed HG-induced ferroptosis and restored osteogenic differentiation (<em>P</em> &lt; 0.05). SIRT1 was shown to bind ELAVL1, and its overexpression suppressed ELAVL1(<em>P</em> &lt; 0.05). In HG-stimulated cells co-overexpressing SIRT1 and ELAVL1, both ferroptosis and osteogenic markers resembled those in cells exposed to HG (<em>P</em> &lt; 0.05). DOP mice exhibited elevated FBG, reduced body weight, increased ELAVL1, and decreased SIRT1(<em>P</em> &lt; 0.05); these mice showed impaired femoral histology. Ferroptosis was evident in DOP mice (<em>P</em> &lt; 0.05). Silencing ELAVL1 improved femoral tissue integrity, inhibited ferroptosis, promoted osteogenic differentiation, restored body weigh, and upregulated SIRT1 in DOP mice (<em>P</em> &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>ELAVL1 repressed SIRT1 translation to interrupt ferroptosis-mediated osteoblastic differentiation, thereby inhibiting DOP progression.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"97 ","pages":"Article 103060"},"PeriodicalIF":2.5,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144757521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}