Tissue & cell最新文献_第10页

Effects of Germanium embedded fabric on the chondrogenic differentiation of adipose derived stem cells 锗嵌入织物对脂肪干细胞软骨分化的影响

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-07-30 DOI: 10.1016/j.tice.2024.102507

Claudia Duranti , Giacomo Bagni , Jessica Iorio , Rossella Colasurdo , Valentina Devescovi , Annarosa Arcangeli

{"title":"Effects of Germanium embedded fabric on the chondrogenic differentiation of adipose derived stem cells","authors":"Claudia Duranti , Giacomo Bagni , Jessica Iorio , Rossella Colasurdo , Valentina Devescovi , Annarosa Arcangeli","doi":"10.1016/j.tice.2024.102507","DOIUrl":"10.1016/j.tice.2024.102507","url":null,"abstract":"<div><p>Osteoarthritis (OA) is a clinical state which is identified by the degeneration of articular cartilage. OA is a common condition (>500 millions of people affected worldwide), whose frequency is anticipated to continue to rise (> 110 % increase worldwide since 2019). The treatment for early-stage OA is based on a combination of therapeutic approaches, which can include regenerative medicine based on Adipose Derived Stem Cells (ADSCs). Germanium embedded Incrediwear® functional Cred40 fabric has been shown to have positive effects on OA clinically and is envisaged to give encouraging effects also on tissue regeneration. Still, the biological mechanisms underlying this therapeutic modality have not yet been fully defined. We tested the hypothesis that Germanium-embedded Incrediwear® functional Cred40 fabric could enhance chondrogenic differentiation. To this purpose, we applied Incrediwear® to human adipose-derived stem cells (hADSCs) induced to chondrogenic differentiation in vitro. Chondrogenic markers (ACAN, SOX9, RUNX2, COL2A1, COL10A1) were quantified following 21 days of treatment. We also assessed extracellular matrix (ECM) deposition (specifically Collagen and glycosaminoglycans (GAGs)) using Alcian Blue and Sirius Red staining.</p><p>Here, we provide pilot data to demonstrate that Germanium-embedded Incrediwear® functional Cred40 fabric can enhance hADSCs chondrogenic differentiation and maturity and potentially induce events of cartilage regeneration.</p></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0040816624002088/pdfft?md5=5f73a1c463658fb094a98884d464c23e&pid=1-s2.0-S0040816624002088-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141917494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the epidermal architecture of Dasyprocta prymnolopha: A potential dermatology research model 探索 Dasyprocta prymnolopha 的表皮结构：潜在的皮肤病学研究模型

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-07-30 DOI: 10.1016/j.tice.2024.102500

Yago Gabriel da Silva Barbosa , Ralph Santos-Oliveira , Luciana Magalhães Rebelo Alencar , Fernando Vagner Lobo Ladd , Fabiane Leite da Silva , Ana Gabriellen Souza do Nascimento , Hermínio José da Rocha Neto , Rebecca Ingryd Coelho de Freitas , Maria Acelina Martins de Carvalho , Napoleão Martins Argôlo Neto

{"title":"Exploring the epidermal architecture of Dasyprocta prymnolopha: A potential dermatology research model","authors":"Yago Gabriel da Silva Barbosa , Ralph Santos-Oliveira , Luciana Magalhães Rebelo Alencar , Fernando Vagner Lobo Ladd , Fabiane Leite da Silva , Ana Gabriellen Souza do Nascimento , Hermínio José da Rocha Neto , Rebecca Ingryd Coelho de Freitas , Maria Acelina Martins de Carvalho , Napoleão Martins Argôlo Neto","doi":"10.1016/j.tice.2024.102500","DOIUrl":"10.1016/j.tice.2024.102500","url":null,"abstract":"<div><p>The agouti (<em>Dasyprocta prymnolopha</em>) is a medium-sized, wild rodent that is highly rustic and docile. Its size and ease of management make it a viable candidate for an alternative animal model to traditional murine subjects. However, data on the epidermal strata of agoutis are lacking, with significant uncertainties persisting regarding their skin’s characterization. This study aimed to describe and quantify the epidermal strata of skin biopsies from male and female agoutis raised in captivity, to further validate the species as a model for dermatological research. Ultrastructural evaluations through atomic force microscopy (AFM) and stereological analyses were conducted, revealing significant differences between the layers of the skin; notably, the dermis exhibited a greater total volume than the epidermis. The findings suggest that the epidermal strata are well-defined, with the volume likely correlating to the size and cellular density of the keratinocytes. Corneodesmosomes and tonofilaments were identified across all epidermal layers, indicating the probable maintenance of anchoring protein activity, even post-cornification of these cells. These results suggest that the agouti may serve as a promising model for dermatological studies, owing to the homogeneity of its cutaneous tissue across different body regions and the distinct volume and morphology of its epithelial stratification, which could enhance the applicability of systematic investigative methods in the future</p></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142040309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The influence of Type I and III collagen on the proliferation, migration and differentiation of myoblasts I 型和 III 型胶原蛋白对肌母细胞增殖、迁移和分化的影响。

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-07-30 DOI: 10.1016/j.tice.2024.102506

Duanyang Wang , Feifan Chang , Zhikang Guo , Ming Chen , Taojin Feng , Mingming Zhang , Xiang Cui , Yuheng Jiang , Jia Li , Yi Li , Jinglong Yan

{"title":"The influence of Type I and III collagen on the proliferation, migration and differentiation of myoblasts","authors":"Duanyang Wang , Feifan Chang , Zhikang Guo , Ming Chen , Taojin Feng , Mingming Zhang , Xiang Cui , Yuheng Jiang , Jia Li , Yi Li , Jinglong Yan","doi":"10.1016/j.tice.2024.102506","DOIUrl":"10.1016/j.tice.2024.102506","url":null,"abstract":"<div><p>Myoblast is a kind of activated muscle stem cell. Its biological activities, such as proliferation, migration, differentiation, and fusion, play a crucial role in maintaining the integrity of the skeletal muscle system. These activities of myoblasts can be significantly influenced by the extracellular matrix. Collagen, being a principal constituent of the extracellular matrix, substantially influences these biological activities. In skeletal muscle, collagen I and III are two kinds of primary collagen types. Their influence on myoblasts and the difference between them remain ambiguous. The purpose of this study is to discover the influence of collagen I and III on biological function of myoblasts and compare their differences. We used C2C12 cell line and primary myoblasts to discover the effect of collagen I and III on proliferation, migration and differentiation of myoblasts and then performed the transcriptome sequencing and analysis. The results showed that both collagen I and III enhanced the proliferation of myoblasts, with no statistical difference between them. Similarly, collagen I and III enhanced the migration of myoblasts, with collagen I was more pronounced in Transwell assay. On the contrary, collagen I and III inhibited myoblasts differentiation, with collagen III was more pronounced at gene expression level. The transcriptome sequencing identified DEGs and enrichment analysis elucidated different terms between Type I and III collagen. Collectively, our research preliminarily elucidated the influence of collagen I and III on myoblasts and their difference and provided the preliminary experimental foundation for subsequent research.</p></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0040816624002076/pdfft?md5=cbf9d33a4276d0b2dee158e4a287e957&pid=1-s2.0-S0040816624002076-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141890180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ENO1 regulates IL-1β-induced chondrocyte inflammation, apoptosis and matrix degradation possibly through the potential binding to CRLF1 ENO1可能通过与CRLF1的潜在结合来调节IL-1β诱导的软骨细胞炎症、凋亡和基质降解。

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-07-30 DOI: 10.1016/j.tice.2024.102504

Zhihua Lu , Dandan Wang , Yuzhe Sun , Yan Dai

{"title":"ENO1 regulates IL-1β-induced chondrocyte inflammation, apoptosis and matrix degradation possibly through the potential binding to CRLF1","authors":"Zhihua Lu , Dandan Wang , Yuzhe Sun , Yan Dai","doi":"10.1016/j.tice.2024.102504","DOIUrl":"10.1016/j.tice.2024.102504","url":null,"abstract":"<div><p>In this study, we aim to investigate the role of enolase 1 (ENO1) in osteoarthritis (OA) pathogenic process and to uncover the underlying mechanism. To this end, we used IL-1β to induce an <em>in vitro</em> OA‑like chondrocyte model in human immortalized chondrocyte C-28/I2 cells. We manipulated the expression of ENO1 and cytokine receptor-like factor 1 (CRLF1) in IL-1β-induced C-28/I2 cells using siRNA and/or overexpression and tested their effects on IL-1β-induced pathologies including cell viability, apoptosis and inflammatory cytokine levels (IL-6 and TNF-α), and the expression of extracellular matrix-related enzymes and major mediators in the NF-κB signaling pathway (p-p65, p65, p-IκBα and IκBα). We used co-immunoprecipitation and immunofluorescence imaging to study a possible binding between ENO1 and CRLF1. Our data showed that IL-1β induction elevated ENO1 and CRLF1 expression in C-28/I2 cells. Silencing ENO1 or CRLF1 inhibited the IL-1β-induced cell viability damage, apoptosis, inflammation, and extracellular matrix degradation. The inhibitory effect of silencing ENO1 was reversed by CRLF1 overexpression, suggesting a functional connection between ENO1 and CRLF1, which could be attributed to a binding between these two partners. Our study could help validate the role of ENO1 in OA pathogenies and identify novel therapeutic targets for OA treatment.</p></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of therapeutic efficacy of adipose tissue-derived mesenchymal stem cells administration in hyperlipidemia-induced aortic atherosclerosis in adult male albino rats 评估脂肪组织间充质干细胞对高脂血症诱导的成年雄性白化大鼠主动脉粥样硬化的疗效。

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-07-29 DOI: 10.1016/j.tice.2024.102498

Rania A. Galhom , Saleh Nasser Saleh Ali , Magdy Mohamed Omar El-Fark , Mona Hassan Mohammed Ali , Hoda Hassan Hussein

{"title":"Assessment of therapeutic efficacy of adipose tissue-derived mesenchymal stem cells administration in hyperlipidemia-induced aortic atherosclerosis in adult male albino rats","authors":"Rania A. Galhom , Saleh Nasser Saleh Ali , Magdy Mohamed Omar El-Fark , Mona Hassan Mohammed Ali , Hoda Hassan Hussein","doi":"10.1016/j.tice.2024.102498","DOIUrl":"10.1016/j.tice.2024.102498","url":null,"abstract":"<div><p>Atherosclerosis (AS) is a common disease seriously detrimental to human health. AS is a chronic progressive disease related to inflammatory reactions. The present study aimed to characterize and evaluate the effects of adipose tissue stem cells (ADSCs) in high-fat diet-induced atherosclerosis in a rat model. The present study comprises thirty-six rats and they were divided into three groups: the control group, the high-fat diet (HFD) group; which received a high-fat diet, and the high-fat diet + stem cells (HFD+SC) group; which was fed with a high-fat diet along with the administration of intravenous ADSCs. Food was given to the animals for 20 weeks to establish dyslipidemia models. After 20 weeks, animals were sacrificed by cervical dislocation; blood was collected to measure total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL), and high-density lipoprotein (HDL); aortae were collected to detect morphologic changes. Rats of the HFD group showed a significant increase in body weight (B.Wt), altered lipid profile increased expression of inducible nitric oxide synthase (iNOS), and decreased expression of endothelial nitric oxide synthase (eNOS). However, in HFD+SC there was a significant decrease in body weight gain and an improvement in lipid profile. Histopathological and ultrastructural variations observed in the aorta of the HFD group when treated with ADSCs showed preserved normal histological architecture and reduced atherosclerosis compared with the HFD group. This was evidenced by laboratory, histological, immunohistochemical, and morphometric studies. Thus, ADSCs reduced TC, TG, and LDL, reduced the expression of iNOS, and increased the expression of eNOS. The high-fat diet was likely to cause damage to the wall of blood vessels. Systemically transplanted ADSCs could home to the aorta, and further protect the aorta from HFD-induced damage.</p></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141856565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PTPN22 through the regulation of Th17/Treg balance acts as a potential target for the treatment of Graves' disease PTPN22通过调节Th17/Treg平衡成为治疗巴塞杜氏病的潜在靶点

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-07-27 DOI: 10.1016/j.tice.2024.102502

Huiyao Cai, Siying Chen, Zhengrong Jiang, Lijun Chen, Xinna Yang

{"title":"PTPN22 through the regulation of Th17/Treg balance acts as a potential target for the treatment of Graves' disease","authors":"Huiyao Cai, Siying Chen, Zhengrong Jiang, Lijun Chen, Xinna Yang","doi":"10.1016/j.tice.2024.102502","DOIUrl":"10.1016/j.tice.2024.102502","url":null,"abstract":"<div><p>Graves' disease (GD) is an autoimmune disease and the most common cause of hyperthyroidism. While the phosphotyrosine phosphatase non-receptor type 22 (PTPN22) variant is associated with GD susceptibility, its precise role and mechanism in GD remain unclear. To investigate this, we induced GD in mice using Ad-TSHR289 and isolated CD4+ T cells from spleen tissues. We conducted a series of experiments, including hematoxylin-eosin staining, enzyme-linked immunosorbent assay (ELISA), immunohistochemistry, flow cytometry, immunofluorescence (IF), reverse transcription quantitative PCR (RT-qPCR), and western blotting. PTPN22 expression was found to be downregulated in GD mice. Overexpression of PTPN22 ameliorated pathological damage and increased serum levels of T4 and thyroid stimulating hormone receptor antibody (TRAb), as well as the ratio of thyroid weight to body weight in GD mice. Furthermore, GD mice exhibited elevated levels of CD4+ and IL-17+ T cells, an increased Th17/Treg ratio, and upregulation of IL-17A mRNA expression. Conversely, there was a decrease in Foxp3+ T cells and transcriptional levels of Foxp3, which were reversed by PTPN22 overexpression. <em>In vitro</em> experiments showed that PTPN22 overexpression in CD4+ T cells from spleen tissues of GD mice enhanced Foxp3 expression while reducing IL-17A expression. Mechanistically, PTPN22 overexpression led to decreased levels of phosphorylated Lck (p-Lck), Lck, phosphorylated Fyn (p-Fyn), Fyn, phosphorylated Zap70 (p-Zap70), and Zap70 in both <em>in vivo</em> and <em>in vitro</em> GD models. In summary, PTPN22 can alleviate thyroid dysfunction in GD by modulating Th17/Treg balance through the downregulation of the Lck/Zap70 signaling axis.</p></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141850420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of macrophages combined with supernatant of mesenchymal stem cell culture and macrophage culture on wound healing in rats 巨噬细胞与间充质干细胞培养液和巨噬细胞培养液相结合对大鼠伤口愈合的影响

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-07-26 DOI: 10.1016/j.tice.2024.102474

Nima Mozaffari , Rahim Mohammadi , Nowruz Delirezh , Rahim Hobbenaghi , Vahid Mohammadi

{"title":"Effect of macrophages combined with supernatant of mesenchymal stem cell culture and macrophage culture on wound healing in rats","authors":"Nima Mozaffari , Rahim Mohammadi , Nowruz Delirezh , Rahim Hobbenaghi , Vahid Mohammadi","doi":"10.1016/j.tice.2024.102474","DOIUrl":"10.1016/j.tice.2024.102474","url":null,"abstract":"<div><p>Wound healing is an orderly sequence of events restoring the integrity of the damaged tissue. It consists of inflammatory, proliferation, and remodeling phases. The objective of the current study was to investigate the effect of local transplantation of cultured macrophage loaded with mesenchymal stem cell/macrophage culture supernatants on wound healing. Sixty-four healthy adult male Wistar rats were randomized into 4 groups of sixteen animals each: 1) SHAM group. 2) MAC-MSC/SN group: One-milliliter application of a mixture comprising mesenchymal stem cell and macrophage culture supernatants in a 1:1 ratio was administered locally to the wound bed. 3) MAC group: Local transplantation of macrophage cells cultured in the wound bed. 4) MAC + MAC-MSC/SN group: Local transplantation of cultured macrophage in combination with mesenchymal stem cell/ macrophage culture supernatants in the wound bed. An incisional wound model was used for biomechanical studies, while an excisional wound model was used for biochemical, histopathological, and planimetric assessments. The wound area was significantly reduced in the MAC + MAC-MSC/SN group compared to other groups (<em>P <</em> 0.05). Biomechanical measurements from the MAC + MAC-MSC/SN group were significantly higher compared to other experimental groups (<em>P <</em> 0.05). Biochemical and quantitative histopathological analyses revealed a significant difference between MAC + MAC-MSC/SN and other groups (<em>P <</em> 0.05). MAC + MAC-MSC/SN showed the potential to improve wound healing significantly. This appears to work by angiogenesis stimulation, fibroblast proliferation, inflammation reduction, and granulation tissue formation during the initial stages of the healing process. This accelerated healing leads to earlier wound area reduction and enhanced tensile strength of the damaged area due to the reorganization of granulation tissue and collagen fibers.</p></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141844540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Momordica cochinchinensis extract alleviates oxidative stress and skin damage caused by fine particulate matter 秦艽提取物可减轻氧化应激和细颗粒物对皮肤的损伤

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-07-25 DOI: 10.1016/j.tice.2024.102496

Seok-Hui Lee , Eun-Ju Kim , Seo-Young Ju , Yong Li , Sei-Jung Lee

{"title":"Momordica cochinchinensis extract alleviates oxidative stress and skin damage caused by fine particulate matter","authors":"Seok-Hui Lee , Eun-Ju Kim , Seo-Young Ju , Yong Li , Sei-Jung Lee","doi":"10.1016/j.tice.2024.102496","DOIUrl":"10.1016/j.tice.2024.102496","url":null,"abstract":"<div><p><em>Momordica cochinchinensis</em> (MC), commonly known as gac fruit, is a tropical fruit rich in antioxidants and bioactive compounds. This research aimed to elucidate the effect of MC on apoptosis induced by fine particulate matter with a diameter of less than 10 μm (< PM<sub>10</sub>) in epidermal keratinocyte HaCaT cells. We found that PM<sub>10</sub> significantly diminish the viability of HaCaT cells through cytotoxic mechanisms. However, the treatment with MC at a concentration of 10 μg/mL notably restored the cellular viability decreased by PM<sub>10</sub>. MC reduced the activation of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (MAPK) by mainly preventing the generation of reactive oxygen species (ROS) in HaCaT cells subjected to PM<sub>10</sub>. Furthermore, MC exhibited a regulatory effect on the expression of genes associated with apoptosis, including B-Cell Lymphoma 2 (Bcl-2), Bcl-2 associated X protein (Bax), and cleaved caspase-3 by inhibiting the activation of the transcription factor nuclear factor-kappa B (NF-κB). These findings demonstrate that MC aids in neutralizing the apoptotic signaling pathway of free radicals produced by environmental pollutants such as PM<sub>10</sub>, which have the potential to damage skin cells and accelerate the aging process.</p></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141850775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Collagen as the extracellular matrix biomaterials in the arena of medical sciences 胶原蛋白是医学领域的细胞外基质生物材料。

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-07-24 DOI: 10.1016/j.tice.2024.102497

Ramachandregowda Sowbhagya , Harsha Muktha , Thippenahalli Narasimhaiah Ramakrishnaiah , Adagur Sudarshan Surendra , Subhas Madinoor Sushma , Chandrashekar Tejaswini , Karunakaran Roopini , Somashekara Rajashekara

引用次数: 0

Dermal derived matrix hydrogel loaded with curcumin improved wound healing in a diabetic rat model 负载姜黄素的真皮衍生基质水凝胶改善了糖尿病大鼠模型的伤口愈合

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-07-23 DOI: 10.1016/j.tice.2024.102495

Suad A. Alghamdi , Mohammed Alissa , Meshari A. Alsuwat

{"title":"Dermal derived matrix hydrogel loaded with curcumin improved wound healing in a diabetic rat model","authors":"Suad A. Alghamdi , Mohammed Alissa , Meshari A. Alsuwat","doi":"10.1016/j.tice.2024.102495","DOIUrl":"10.1016/j.tice.2024.102495","url":null,"abstract":"<div><p>There is a need in clinical practice for new wound healing techniques to address full thickness skin injuries, particularly in individuals with diabetes. Herein we investigated whether dermal derived matrix hydrogel (DMH) loaded with curcumin (Cur) could promote healing in diabetic rats. Sixty diabetic rats were randomly assigned into the non-treated group, DMH group, Cur group, and DMH+Cur group. According to the phases of wound healing, sampling was done on days 7, 14, and 21 for further assessments. Our results indicated that the wound contraction rate, new epidermal length and thickness, number of fibroblasts and vascular length, collagen deposition, and strength properties of the healed wounds were meaningfully increased in the treatment groups than in the non-treated group, and these changes were more obvious in the DMH+Cur ones. In addition, the expression of VEGF and IL-10 genes were meaningfully upregulated in all treatment groups compared to the non-treated group and were greater in the DMH+Cur group. This is while the number of neutrophils and expression levels of TNF-α and IL-1β genes decreased more significantly in the DMH+Cur group compared to the other groups. In conclusion, it was found that using both DMH and curcumin has a greater impact on diabetic wound healing.</p></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141845737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0