Resveratrol protects against letrozole-induced renal damage in a rat model of polycystic ovary syndrome: A biochemical, histological, and immunohistochemical study

IF 2.7 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-04-26 DOI:10.1016/j.tice.2025.102934

Einas M. Yousef , Samar M. Abd El-moneam , Shimaa Mohammad Yousof , Safaa Abdallah Mohammed , Basma Osman Sultan , Basma S.A. Mansour

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引用次数: 0

Abstract

Background

Polycystic ovary syndrome (PCOS) is a common endocrine disorder that affects 9–18 % of women, often associated with metabolic and renal complications. This study aimed to investigate the renoprotective effects of resveratrol (RSV) in a letrozole-induced rat model of PCOS, focusing on biochemical, histological, ultrastructural, and immunohistochemical alterations.

Methods

Thirty female rats were randomly divided into five groups: negative control, only RSV-treated, PCOS-induced (sham), metformin-treated, and RSV-treated. Serum testosterone, urea, and creatinine levels were assessed. Renal tissues underwent histological, immunohistochemical, ultrastructural, and morphometric analyses. Additionally, TGF-β1 mRNA expression was evaluated using qRT-PCR.

Results

Letrozole administration significantly elevated serum testosterone, urea, and creatinine levels, indicating PCOS-associated renal dysfunction. Histological and ultrastructural analysis revealed severe glomerular and tubular alterations in the sham group. The administration of RSV significantly restored renal architecture and function more effectively than metformin. Immunohistochemistry analysis showed that RSV reduced Proliferating Cell Nuclear Antigen (PCNA) overexpression and restored B-cell Lymphoma 2 (BCL-2) expression, suggesting a protective effect against cellular stress and apoptosis. Moreover, RSV significantly downregulated TGF-β1 expression, indicating its anti-fibrotic and anti-inflammatory role in PCOS-related renal damage.

Conclusion

In a PCOS rat model, RSV protects effectively against letrozole-induced structural and functional renal damage. It demonstrates superior efficacy over metformin in restoring renal function, reducing apoptosis, and mitigating fibrosis. These findings suggest that RSV may serve as a potential adjunct therapy for preventing PCOS-associated renal complications, emphasizing the need for further investigations.

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白藜芦醇对多囊卵巢综合征大鼠模型来曲唑诱导的肾脏损伤有保护作用：生化、组织学和免疫组织化学研究

背景：多囊卵巢综合征（PCOS）是一种常见的内分泌疾病，影响9 - 18% %的女性，通常伴有代谢和肾脏并发症。本研究旨在探讨白藜芦醇（resveratrol， RSV）在来曲唑诱导的PCOS大鼠模型中的肾保护作用，主要从生化、组织学、超微结构和免疫组织化学等方面进行观察。方法30只雌性大鼠随机分为阴性对照组、单纯rsv治疗组、pcos诱导组（假组）、二甲双胍治疗组和rsv治疗组。测定血清睾酮、尿素和肌酐水平。肾组织进行了组织学、免疫组织化学、超微结构和形态计量学分析。qRT-PCR检测TGF-β1 mRNA表达。结果曲唑组血清睾酮、尿素和肌酐水平明显升高，提示pcos相关肾功能不全。组织学和超微结构分析显示假手术组肾小球和肾小管有严重改变。RSV比二甲双胍更有效地恢复肾脏结构和功能。免疫组化分析显示，RSV降低了增殖细胞核抗原（PCNA）的过表达，恢复了b细胞淋巴瘤2 （BCL-2）的表达，提示RSV对细胞应激和凋亡具有保护作用。RSV显著下调TGF-β1表达，提示其在pcos相关肾损害中具有抗纤维化和抗炎作用。结论在PCOS大鼠模型中，RSV对来曲唑所致的肾脏结构和功能损害具有保护作用。它在恢复肾功能、减少细胞凋亡和减轻纤维化方面优于二甲双胍。这些发现表明RSV可能作为预防pcos相关肾脏并发症的潜在辅助治疗，强调需要进一步研究。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.