Oxygen-glucose deprivation induces actin spillover in brain endothelial cells

IF 2.7 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-04-29 DOI:10.1016/j.tice.2025.102946

Yiyin Zhao , Xiaojing Zhou , Songbin He , Jingjing Liu , Meng Jin , Jiaqian Li , Lulan Pan , Lin Zhou

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引用次数: 0

Abstract

Stroke is the leading cause of death and disability worldwide, and the mechanisms of stroke onset have not been fully elucidated. The research investigated how actin remodeling functions within brain microvascular endothelial cells (bEnd.3 cell²) when exposed to glucose-oxygen deprivation (OGD³) circumstances. OGD exposure for 6 h in bEnd.3 cell led to increased F-actin polymerization and actin overflow into the supernatant which demonstrated a disruption of intracellular actin balance. This process is mainly mediated by the cofilin and myosin light chain (MLC⁴) phosphorylation. Jasplakinolide further enhanced F-actin polymerization, while Latrunculin B inhibited actin polymerization and alleviated cellular damage. In conclusion, our research has revealed the crucial role of actin overflow driven by cofilin and MLC signals in brain endothelial cell injury, providing new insights into the pathophysiology of stroke.

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氧葡萄糖剥夺诱导脑内皮细胞肌动蛋白溢出

脑卒中是世界范围内导致死亡和残疾的主要原因，其发病机制尚未完全阐明。研究了肌动蛋白重塑在脑微血管内皮细胞（bEnd）内的功能。当暴露于葡萄糖-氧气剥夺（OGD3）的情况下。在bEnd中OGD暴露6 h。3细胞导致f -肌动蛋白聚合增加，肌动蛋白溢出到上清中，表明细胞内肌动蛋白平衡被破坏。这一过程主要由cofilin和myosin轻链（MLC4）磷酸化介导。茉莉烯内酯进一步促进了f -肌动蛋白的聚合，而拉曲库林B则抑制了肌动蛋白的聚合，减轻了细胞损伤。总之，我们的研究揭示了cofilin和MLC信号驱动的肌动蛋白溢出在脑内皮细胞损伤中的重要作用，为卒中的病理生理学提供了新的见解。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.