Tissue & cell最新文献

{"title":"Investigating the enhancement of neural differentiation of adipose-derived mesenchymal stem cell with Foeniculum vulgare nanoemulsions: An in vitro research","authors":"Arya Mojtahedi ,&nbsp;Shima Ghaderi ,&nbsp;Mohsen Ghiasi ,&nbsp;Raheleh Halabian ,&nbsp;Hossein Dehghan ,&nbsp;Arash Padash ,&nbsp;Elahe Eftekhari ,&nbsp;Ali Salimi","doi":"10.1016/j.tice.2025.102806","DOIUrl":"10.1016/j.tice.2025.102806","url":null,"abstract":"<div><h3>Background</h3><div>Neurons, distributed throughout the body, regulate various bodily functions. The recovery of the nervous system is often slow and can be irreversible. Currently, the approach of using mesenchymal stem cells (MSCs) in conjunction with conventional treatments for nervous system injuries is being explored. Nanoemulsions are systems designed for the nanoscale delivery of drug cargoes. <em>Foeniculum vulgare (F. vulgare)</em>, a medicinal plant long utilized in complementary medicine, is the focus of this study. The aim is to utilize nanoemulsions of fennel to induce the differentiation of MSCs into neural-like cells <em>in vitro</em>.</div></div><div><h3>Materials and methods</h3><div>Human adipose-derived mesenchymal stem cells (hADSCs) were commercially purchased. These cells were cultured in DMEM medium containing 10 % fetal bovine serum and 1 % penicillin-streptomycin antibiotic. Based on a sequential extraction method, n-hexane (Hex), ethyl acetate (EtAc), and ethanolic extracts were obtained from the seeds of <em>F. vulgare</em>. To prepare the <em>F. vulgare</em> extract nanoemulsion, the aqueous phase (distilled water), the oily part (<em>F. vulgare</em> extract), Span 80 and Tween 20 were used. The optimal dose of <em>F. vulgare</em> nanoemulsion was determined using the MTT assay and acridine orange/ethidium bromide (AO/EB) staining. Neural differentiation was induced using a specialized differentiation medium on the MSCs, with the prepared nanoemulsions acting as inducers. The neural differentiation of the human differentiated hADSCs was studied and evaluated through Real-time PCR and immunocytochemistry (ICC) techniques on days 7 and 14.</div></div><div><h3>Results</h3><div>The results obtained from the MTT and AO/EB tests indicated that the optimal dose of <em>F. vulgare</em> nanoemulsions is 1 μg/ml. Analysis of neural differentiation index gene expression revealed a significant (P ≤ 0.05) upregulation of <em>MAP-2</em>, <em>β-tubulin III</em>, and <em>NSE</em> genes on days 7 and 14 following treatment with the nanoemulsions. It is noteworthy that the nanoemulsion prepared from the hexane extract of the plant showed a significant increase in the expression of marker genes in the process of neural differentiation. Protein expression analysis demonstrated an increase in MAP-2, β-tubulin III, and NSE (gamma enolase) proteins in response to the nanoemulsion inducers compared to the control group (TCPS).</div></div><div><h3>Discussion</h3><div>Overall, our findings indicate that <em>F. vulgare</em> nanoemulsions have a positive effect on the expression of genes and proteins related to neural differentiation in hADSCs. The proposed protocol may serve as a potential therapeutic strategy in complementary medicine for patients seeking to improve injuries to the nervous system. However, further studies and performance measurements are necessary in future research to confirm these results.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"94 ","pages":"Article 102806"},"PeriodicalIF":2.7,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143520712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roxadustat pre-conditioning and cyclic uniaxial stretching improve tenogenic differentiation potential of human adipose derived mesenchymal stromal cells","authors":"Amirah Zulkifli ,&nbsp;Hui Yin Nam ,&nbsp;Wuey Min Ng ,&nbsp;Nor Faissal Yasin ,&nbsp;Tunku Kamarul","doi":"10.1016/j.tice.2025.102828","DOIUrl":"10.1016/j.tice.2025.102828","url":null,"abstract":"<div><div>Tendon injuries represent a significant challenge to treat owing to their limited intrinsic reparative capacity. The use of mesenchymal stem cells (MSC) offers promising alternative therapeutic option to augments tendon repair. It is hypothesised that the activation of hypoxia inducible factor-1 alpha (HIF-1α), could facilitate the tendon repair process by promoting the proliferation and tenogenic differentiation of MSCs. To demonstrate this, a study was conducted incorporating the use of Roxadustat, a specific hypoxia mimetic mediator and cyclic uniaxial stretching at a frequency of 1 Hz and 8 % strain on adipose derived-mesenchymal stromal cells (ADMSCs). Methods: Cellular morphology, proliferation rate, tenogenic protein and gene expression levels from 8 different treatment groups were compared. These groups include untreated ADMSCs (Control), Roxadustat pre-conditioned ADMSCs (ROX), ADMSCs subjected CAY10585 treatment only (CAY), Roxadustat pre-conditioned ADMSCs with CAY10585 inhibition (ROX+CAY), ADMSCs subjected to uniaxial stretching only (S), Roxadustat pre-conditioned ADMSCs with uniaxial stretching (ROX+S), ADMSCs subjected CAY10585 with uniaxial stretching (CAY+S) and primary tenocytes (Tenocytes). Results: ROX+S group exhibited the highest expression of HIF-1α and demonstrated a significant up-regulation of collagen I and III expressions, increasing by 4.9 and 5.6-fold compared to ROX group, respectively. There is a significant increase of <em>SCX, TNC, TNMD, COLI</em> and <em>COLIII</em> expression in this combination treatment group; (<em>SCX</em>= 9.9, <em>TNC</em>= 12.6, <em>TNMD</em>= 7.0, <em>COLI</em>= 8.0 and <em>COLIII</em>= 10.0-fold). Conversely, the expression of the markers markedly reduced with HIF-1α inhibitor CAY10585. However, uniaxial stretching effectively counteracted the inhibitory effects of CAY10585 in the CAY+ S group, resulting in a 3.9-fold increase in <em>SCX</em> expression compared to CAY treatment alone. Conclusion: HIF-1α accumulation promotes superior tenogenic differentiation of ADMSCs, suggesting that the combination of Roxadustat and cyclic uniaxial stretching may be a potential therapeutic mediator in tendon repair strategies.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"95 ","pages":"Article 102828"},"PeriodicalIF":2.7,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143620390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Micronized cellular adipose matrix purified with a bladed connector contains abundant functional adipose stem cells” [Tissue Cell 89 (2024) 102457]","authors":"Yoshihiro Sowa ,&nbsp;Seiji Sawai ,&nbsp;Kenta Yamamoto ,&nbsp;Ataru Sunaga ,&nbsp;Natsumi Saito ,&nbsp;Takako Shirado ,&nbsp;Yoshihiro Toyohara ,&nbsp;Li Bolun ,&nbsp;Kotaro Yoshimura ,&nbsp;Osam Mazda","doi":"10.1016/j.tice.2025.102810","DOIUrl":"10.1016/j.tice.2025.102810","url":null,"abstract":"","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"94 ","pages":"Article 102810"},"PeriodicalIF":2.7,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of systemic vitamin D administration and local photobiomodulation on bone repair: Histomorphometric, histochemical, and immunohistochemical study in rat calvaria","authors":"Carolina dos Santos Santinoni ,&nbsp;Wesley Fonseca Novaes ,&nbsp;Marcela Lucio Caldeira ,&nbsp;Graziela Garrido Mori ,&nbsp;Liliane Aparecida Tanus Benatti ,&nbsp;Maurício Malheiros Badaró ,&nbsp;Juliana Ribeiro de Andrade ,&nbsp;Thaís Mageste Duque ,&nbsp;Gabriel Leonardo Magrin ,&nbsp;César Augusto Magalhães Benfatti ,&nbsp;Victor Eduardo de Souza Batista ,&nbsp;Edilson Ervolino","doi":"10.1016/j.tice.2025.102814","DOIUrl":"10.1016/j.tice.2025.102814","url":null,"abstract":"<div><h3>Objective</h3><div>The purpose of the present study was to evaluate the influence of systemic vitamin D (Vit D) administration with or without local photobiomodulation (PBM) on bone repair of surgically created critical-size defects (CSD) in rat calvaria.</div></div><div><h3>Material and method</h3><div>Thirty-two rats were used. A 5 mm DTC was created on the calvaria of each animal. The animals were randomly distributed into four experimental groups (n = 8): Control: no treatment; Vit D: systemic Vit D administration through gavage; PBM: local irradiation with low-intensity laser therapy; Vit D/PBM: systemic Vit D administration through gavage and local irradiation with low-intensity laser therapy. The animals were euthanized 15 days after treatments. It was evaluated newly formed bone area (NFBA), percentage of mature and immature collagen fibers (picrosirius red staining and polarized light), immunohistochemical identification of tartrate-resistant acid phosphatase (TRAP), transforming growth factor (TGF), and osteocalcin (OCN). Data were statistically analyzed (ANOVA, Kruskal-Wallis, p &lt; 0.05).</div></div><div><h3>Results</h3><div>All experimental groups presented similar statistical results in the evaluated parameters. Some qualitative differences were observed, as follows. Groups PBM, Vit D, and Vit D/ PBM presented a slight amount of newly formed bone. Group Vit D presented a higher amount of mature collagen fibers. Groups Vit D and PBM presented higher immunoexpression of OCN than other experimental groups.</div></div><div><h3>Conclusion</h3><div>It can be concluded that Vit D and PBM did not significantly increase the amount of bone tissue formed but showed a trend towards enhanced bone maturation in the early healing stages of critical-sized defects surgically created in rat calvaria.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"95 ","pages":"Article 102814"},"PeriodicalIF":2.7,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143551954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polygala crotalarioides attenuates erectile dysfunction through improving Rho/ROCK signaling pathway mediated thyroid and gonadal function in kidney Yang deficiency ED rat","authors":"Xizhi Chen ,&nbsp;Yao Chen ,&nbsp;Weibo Wen","doi":"10.1016/j.tice.2025.102812","DOIUrl":"10.1016/j.tice.2025.102812","url":null,"abstract":"<div><div>This study aimed to explore effectiveness and related mechanisms of <em>Polygala crotalarioides</em> in treating erectile dysfunction (ED). Hydrocortisone was used to establish a kidney-<em>Yang</em> deficiency ED rat model. Levels of triiodothyronine(T3), thyroxine(T4), testosterone(T), estradiol(E2) and expressions of RhoA, ROCK1, ROCK2 were determined. Immunofluorescence staining was used to detect CD34 in penile tissues. Compared with normal rats, T3, T4, T, E2 levels in serum of ED rat model were significantly reduced (P &lt; 0.05), while RhoA, ROCK1, ROCK2 expressions were significantly higher (P &lt; 0.05). High- and medium-dose of <em>Polygala crotalarioides</em> significantly attenuated erectile dysfunction, but low-doses of <em>Polygala crotalarioides</em> were ineffective. High and medium dose of <em>Polygala crotalarioides</em> and sildenafil significantly increased weight of ED rats compared to Model group (P &lt; 0.05). T3, T4, E2, and T levels in low-dose, medium-dose, and high-dose of <em>Polygala crotalarioides</em> group were increased compared to Model group (P &lt; 0.05). Compared with Model group, RhoA, ROCK1, and ROCK2 expressions in penile tissue of rats in low-, medium-, high-dose of <em>Polygala crotalarioides</em> group were reduced (P &lt; 0.05). High-, medium-, and low-dose <em>Polygala crotalarioides</em> significantly increased CD34 compared with ED rat (P &lt; 0.05). In conclusion, <em>Polygala crotalarioides</em> attenuated erectile dysfunction and improved thyroid and gonadal function by regulating Rho/ROCK pathway in kidney <em>Yang-</em>deficiency ED rat.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"94 ","pages":"Article 102812"},"PeriodicalIF":2.7,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143552485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Paracentrotus lividus spines extract antioxidant, antidiabetic, anti-inflammatory, antimicrobial, and mechanistic anticancer: Insights into its composition using UPLC-ESI-MS-based metabolomic profiling","authors":"Maha M. Salem ,&nbsp;Mai M. Elkeiy ,&nbsp;Mona M. El-Gamal ,&nbsp;Khalil M. Saad-Allah ,&nbsp;Abeer A. Khamis","doi":"10.1016/j.tice.2025.102808","DOIUrl":"10.1016/j.tice.2025.102808","url":null,"abstract":"<div><div>Sea urchins are widely distributed in waters worldwide<em>.</em> The present study carried out the in vitro metabolomic bioactivity profiling using UPLCMS/MS of <em>Paracentrotus lividus</em> spines extract. Investigations were also conducted on molecular anticancer, anti-inflammatory, antidiabetic, antioxidant, and antibacterial properties. A comprehensive untargeted metabolic profiling of <em>P. lividus</em> spines extract resulted in the classification of more than 13 metabolites. Their metabolomic quantitative evaluations were assumed by measuring total phenolic, flavonoids, dihydroflavonol, sugar, and protein contents. The <em>P. lividus</em> spines extract exhibited powerful antioxidant capacity using DPPH*, ABTS⁺, reducing power, and phosphomolybdate assays. Moreover, <em>P. lividus</em> spines extract highly elucidated antidiabetic and anti-inflammatory activity by inhibiting α-amylase enzyme and protein denaturation. Further, the spines of the <em>P. lividus</em> exhibited significant antibacterial effects. Besides, extract from <em>P. lividus</em> spines showed a strong cytotoxic impact against a variety of HepG-2 and MCF-7 cancer cell lines. It was discovered that the <em>P. lividus</em> spines extract triggered cell cycle arrest in the sub-G0/G1 phase and suppressed the growth of cancer cells via suppressing mRNA of Akt/MAPK/Bcl-2/c-myc and protein expression of β-Catenin/ki-67. Conclusively, the extract derived from the spines of the sea urchin species <em>P. lividus</em> demonstrates significant potential for utilization in various pharmaceutical industries.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"94 ","pages":"Article 102808"},"PeriodicalIF":2.7,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting TLR4/NF-κB signaling, oxidative stress, and apoptosis by farnesol mitigates cadmium-induced testicular toxicity in rats","authors":"Emad H.M. Hassanein ,&nbsp;Mohammed F. Alotaibi ,&nbsp;Reem S. Alruhaimi ,&nbsp;Mostafa Sabry ,&nbsp;Ghadir A. Sayed ,&nbsp;Ahmed M. Atwa ,&nbsp;Ayman M. Mahmoud","doi":"10.1016/j.tice.2025.102813","DOIUrl":"10.1016/j.tice.2025.102813","url":null,"abstract":"<div><div>Cadmium (Cd) is a highly toxic heavy metal, and its detrimental effects on reproductive health pose a significant risk to the general population. Farnesol (FAR), a sesquiterpene alcohol, exhibits anti-inflammatory, antioxidant, and anticancer properties. This study investigated the protective effects of FAR against Cd-induced testicular toxicity, focusing on its antioxidant and anti-inflammatory mechanisms. Rats were randomly divided into four experimental groups: control, FAR (10 mg/kg), Cd (1.2 mg/kg), and Cd + FAR. Cd administration caused testicular tissue damage, altered hormone levels, oxidative stress and apoptosis, upregulated TLR4/NF-κB signaling and diminished antioxidants. FAR ameliorated gonadotropins and testosterone, prevented tissue damage, and attenuated oxidative stress. Additionally, FAR significantly attenuated the inflammatory response triggered by Cd, as evidenced by reduced levels of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) and suppression of the TLR4/NF-κB signaling pathway. FAR inhibited testicular apoptosis by upregulating the anti-apoptotic protein Bcl-2 and downregulating the pro-apoptotic markers Bax and caspase-3. These results suggest that FAR mitigates Cd-induced testicular toxicity through upregulation of antioxidants, suppression of TLR4/NF-κB signaling, and inhibition of apoptotic pathways. Thus, FAR represents a promising therapeutic agent for protecting against Cd-induced reproductive damage.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"94 ","pages":"Article 102813"},"PeriodicalIF":2.7,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143511531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing prostate cancer cells’ sensitivity to flutamide by resveratrol: An in-vitro study","authors":"Seyedeh Fatemeh Hosseini ,&nbsp;Seyed Reza Yahyazadeh ,&nbsp;Akram Mirzaei ,&nbsp;Rahil Mashhadi ,&nbsp;Helia Azodian Ghajar ,&nbsp;Diana Taheri ,&nbsp;Seyed Mohammad Kazem Aghamir","doi":"10.1016/j.tice.2025.102807","DOIUrl":"10.1016/j.tice.2025.102807","url":null,"abstract":"<div><h3>Background</h3><div>As the most common cancer in men, and its progression poses a significant challenge. New and effective treatment strategies are needed to improve outcomes for patients with prostate cancer. This study examined if resveratrol, a natural substance, could improve prostate cancer cell lines' response to flutamide, a standard antiandrogenic treatment for untreated prostate cancer, while minimizing adverse effects.</div></div><div><h3>Method</h3><div>MTT assay was used to quantify resveratrol and flutamide IC50 values, Annexin-V/PI staining for apoptosis, PI staining for DNA cell cycle, and real-time PCR for <em>BAX, BCL-2,</em> VEGFC, <em>HIF-1α</em>, <em>Snail1</em>, <em>E-Cadherin</em>, and <em>KLK3</em> mRNA levels Scratch-wound, colony-forming, and Hoechst staining analyzed cell migration, proliferation, and nucleus morphology. Spheroid creation in 3D was also considered. All tests used LNCaP, DU145, and PC3 prostate cancer cell lines at various stages.</div></div><div><h3>Results</h3><div>Resveratrol, when combined with flutamide, can reduce malignant cell migration, colony formation, and proliferation and promote apoptosis in prostate cancer cell lines. Even in androgen-unresponsive cell lines (DU145 and PC3), it may benefit flutamide prostate cancer treatment. Apoptosis genes (<em>BAX</em>) were upregulated in LNCaP, DU145, and PC3 cancer cell lines when administered alone or with flutamide. Additionally, flutamide might significantly lower <em>BCL-2</em> levels in PC3 cells. When combined with flutamide, resveratrol increased apoptosis and altered the expression of genes involved in angiogenesis (VEGFC), epithelial-mesenchymal transition (<em>EMT, Snail1</em> and <em>E-Cadherin</em>), and prostate cancer biomarker (<em>KLK3</em>) in prostate cancer cell lines.</div></div><div><h3>Conclusion</h3><div>Resveratrol reduced the dose of flutamide in the treatment of prostate cancer cell lines (LNCaP, DU145, and PC3) and improved its side effects, as well as increasing the sensitivity of cells to flutamide treatment.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"94 ","pages":"Article 102807"},"PeriodicalIF":2.7,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143511536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Dental Pulp Stem Cells (DPSCs) and Periodontal Ligament Stem Cells (PDLSCs): Difference in effect of aspirin on osteoblast potential of PDLSCs and DPSCs","authors":"Fazliny Abd. Rahman ,&nbsp;Fatin Nur Azwa","doi":"10.1016/j.tice.2025.102776","DOIUrl":"10.1016/j.tice.2025.102776","url":null,"abstract":"<div><div>Periodontal Ligament Stem Cells (PDLSCs) and Dental Pulp Stem Cells (DPSCs) are mesenchymal stem cells with the ability to self-renew and differentiate into three lineages. One significant advantage of dental stem cells, such as PDLSCs and DPSCs, is their ease of harvest compared to other types of mesenchymal stem cells (MSCs). While MSCs are highly valued in bone tissue engineering, MSCs sourced from dental tissues, such as PDLSCs and DPSCs, offer promising options for periodontal regeneration because they are more easily accessible and can be collected through minimally invasive methods. Currently, PDLSCs and DPSCs exhibit a strong ability to undergo osteogenic differentiation when stimulated by factors such as growth factors, chemicals, and paracrine signaling. It has been shown that aspirin (ASA) can enhance the osteoblastic potential of PDLSCs and DPSCs, although the exact mechanism remains unclear. This article examines the origin and features of mesenchymal stem cells, the bone regeneration potential of DPSCs and PDLSCs, the factors that enhance their osteogenic differentiation, and a comparison of PDLSCs and DPSCs regarding their proliferation and differentiation abilities. Additionally, we will examine the effects of aspirin on PDLSCs and DPSCs. In conclusion, PDLSCs show a greater effect on osteoblast differentiation.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"94 ","pages":"Article 102776"},"PeriodicalIF":2.7,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143520713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Allicin protects against pancreatic damage induced by zearalenone in rats by inhibiting endoplasmic reticulum stress","authors":"Amany Mohamed Shalaby ,&nbsp;Amira Mostafa Elshamy ,&nbsp;Abdulfatah Mohammed Albakkosh ,&nbsp;Sulaiman Mohammed Alnasser ,&nbsp;Mohammed Alorini ,&nbsp;Fatima A. Jaber ,&nbsp;Mohamed Ali Alabiad ,&nbsp;Sabah Mohamed Hanafy ,&nbsp;Nema Soliman ,&nbsp;Shereen Elsayed Tawfeek","doi":"10.1016/j.tice.2025.102802","DOIUrl":"10.1016/j.tice.2025.102802","url":null,"abstract":"<div><div>Zearalenone (ZEL) is a mycotoxin generated by Fusarium fungus. Ingestion of ZEL-contaminated foods by humans or animals can cause major health concerns. This work assessed the protective role of allicin in mitigating pancreatic damage caused by ZEL in rats. The experimental rats were allocated into control, Allicin (45 mg/kg /day), ZEL (20 mg/kg/ day), and Allicin-ZEL groups. The agents were administered orally for six weeks. ZEL enhanced the serum levels of amylase and lipase, oxidative stress parameters, and endoplasmic reticulum (ER) stress biomarkers, along with a marked decrease in the serum level of insulin. The disturbed architecture of pancreatic acini was demonstrated in the form of vacuolation of acini, degenerated acini with pyknotic nuclei, and infiltration around dilated congested blood vessels, in addition to the presence of dilated intralobular ducts with retained secretions. Also, the islet of Langerhans cells showed vacuolation and darkly stained nuclei. Immunohistochemically, a marked rise in the expression of heat shock protein 70 (HSP70) and P53 and a marked decline in insulin expression were demonstrated. Ultrastructurally, the pancreatic acinar cells and islets of Langerhans cells displayed shrunken irregular nuclei with dilated perinuclear cisternae and dilated rER. Interestingly, co-administration of allicin and ZEL greatly mitigated these detrimental effects. In summary, allicin inhibited pancreatic injury induced by ZEL by decreasing oxidative stress, ER stress, and apoptosis.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"94 ","pages":"Article 102802"},"PeriodicalIF":2.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143471180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}