Tissue & cell最新文献

{"title":"Trim33 inhibited the growth and relieved the fibrosis of mesangial cells through inducing the ubiquitination degradation of Smad2","authors":"Zhiyuan Liu,&nbsp;Yong Zhang,&nbsp;Hongzhou Li","doi":"10.1016/j.tice.2025.102915","DOIUrl":"10.1016/j.tice.2025.102915","url":null,"abstract":"<div><h3>Background</h3><div>Diabetic nephropathy (DN) poses a significant global health burden due to its progressive nature leading to end-stage renal disease and increased mortality rates. Here, we explored the effects of Tripartite Motif Containing 33 (Trim33) on cell viability and fibrosis of mesangial cells (MCs).</div></div><div><h3>Methods</h3><div>MCs were treated with 30 mM D-glucose (HG) and mice were injected with 50 mg/kg STZ to establish the DN model. Cell growth were detected by CCK-8 and EdU staining. The protein levels of fibronectin (FN), Collagen IV (Col-IV), α-SMA, Smad2, Smad3 and Smad4 were tested by western blot. Furthermore, the interaction between Trim33 and Smad2 were tested by Immunoprecipitation. Wild-type and mutation type of ubiquitin was used to analyze the ubiquitination levels of Smad2. Finally, the injury of kidneys in DN mice were observed by HE staining.</div></div><div><h3>Results</h3><div>Trim33 was down regulated in HG treated MCs and DN mice. Trim33 verexpression decreased the cell viability and proliferation of MCs. Besides, the protein levels of FN, Col-IV, α-SMA, Smad2, Smad3 and Smad4 were decreased after Trim33 overexpression in vivo and in vitro. Furthermore, CO-IP assay confirmed that Trim33 interacted with Smad2. Trim33 regulated the Smad2 ubiquitination through the K48 site of Ub. Trim33 overexpression induced the protein degradation of Smad2. Additionally, Smad2 overexpression reversed the effects of Trim33 on the growth and fibrosis of HG treated MCs.</div></div><div><h3>Conclusion</h3><div>This study demonstrated that Trim33 overexpression inhibited the growth and fibrosis progression in HG treated MCs through inducing the ubiquitination degradation of Smad2 protein.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"95 ","pages":"Article 102915"},"PeriodicalIF":2.7,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the direct influence of growth hormone on adamantinomatous craniopharyngioma cells","authors":"Zhiwei Xiong ,&nbsp;Kai Li ,&nbsp;Milai Yu ,&nbsp;Zijing Wang ,&nbsp;Yihan Wang ,&nbsp;Rongjun Chen ,&nbsp;Weizhao Li ,&nbsp;Keying Zhang ,&nbsp;Yucong Lin ,&nbsp;Zhixuan Zhang ,&nbsp;Haochuan Meng ,&nbsp;Guowu Pu ,&nbsp;Danling Li ,&nbsp;Yongfu Cao ,&nbsp;Junxiang Peng","doi":"10.1016/j.tice.2025.102892","DOIUrl":"10.1016/j.tice.2025.102892","url":null,"abstract":"<div><h3>Purpose</h3><div>This study aims to investigate the impact of growth hormone therapy on the postoperative management of craniopharyngioma and to evaluate the direct influence of growth hormone (GH) on craniopharyngioma.</div></div><div><h3>Methods</h3><div>The research involved the application of varying concentrations of growth hormone to stimulate and cultivate the adamantinomatous craniopharyngioma (ACP) cell line. It focused on assessing the influence of growth hormone on the growth, proliferation, and apoptosis of ACP cells, and explored changes in transcription levels following growth hormone treatment of ACP cells through transcriptome sequencing. The results shed light on the effects and underlying mechanisms through which growth hormone impacts ACP cells.</div></div><div><h3>Results</h3><div>ACP cells were treated with different concentrations of growth hormone (0.01, 0.05, 0.1, 0.5, 1, 10 μg/ml) for 24, 48, and 72 hours. The CCK8 cell viability assay's results indicate that growth hormone stimulation does not significantly increase ACP cell proliferation. Furthermore, sequencing data analysis suggests that growth hormone does not promote the activation of pathways associated with craniopharyngioma proliferation. Immunofluorescence analysis confirmed the presence of GHR receptors on ACP cells. Moreover, flow cytometry experiments focusing on cell cycle and apoptosis in ACP cells show that growth hormone does not affect cell cycle progression or apoptosis.</div></div><div><h3>Conclusion</h3><div>The findings suggest that growth hormone can impact craniopharyngioma directly but it didn’t promote the growth of ACP.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"95 ","pages":"Article 102892"},"PeriodicalIF":2.7,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamin D alleviates chronic stress-induced testicular steroidogenesis disruption in Wistar rats","authors":"Siddhi Srivastava ,&nbsp;Sukriti Srivastava ,&nbsp;Vipul Agarwal ,&nbsp;Mujeeba Rehman ,&nbsp;Rishabh Chaudhary ,&nbsp;Arjun Singh Kaushik ,&nbsp;Sapana Kushwaha ,&nbsp;Vikas Mishra","doi":"10.1016/j.tice.2025.102910","DOIUrl":"10.1016/j.tice.2025.102910","url":null,"abstract":"<div><div>Stress is associated with various health issues. Research has highlighted the relationship between chronic stress and male reproductive health. One of the primary mechanisms underlying stress-induced male reproductive dysfunction is impaired steroidogenesis. In the present study, we validated a chronic unpredictable stress (CUS) model and investigated testicular dysfunction in CUS rats. The CUS paradigm involved exposing rats to a variety of stressors daily for 8 weeks. Vitamin D (10 µg/kg/twice a week, p.o) was administered to CUS rats starting 2 weeks after the onset of stress exposure and continued until the end of study. The stress in rats was confirmed by the occurrence of anxiety and depressive-like behaviours through elevated plus-maze test &amp; novelty-suppressed feeding test and rise in serum corticosterone levels. Testicular dysfunction in CUS rats was assessed via serum gonadotropins, testosterone, cytokines, oxidative stress, and testis-epididymis-sperm morphology. The reduction in steroidogenesis was confirmed via immunohistochemical analysis of 17β-hydroxysteroid dehydrogenase-3 (17β-HSD3), steroidogenic acute regulatory gene (StAR) and vitamin D receptor (VDR) expression. Further, we studied the role of vitamin D in alleviating stress-induced testicular damage and the potential mechanisms underlying steroidogenic alterations in CUS rats. Notably, vitamin D treatment prevented CUS-induced decline in testicular 17β-HSD3, StAR and VDR expression. Moreover, vitamin D ameliorated the CUS-induced reduction in serum testosterone levels. Histological assessment revealed that vitamin D prevented CUS-induced damage in sperm, testis and epididymis morphology. In conclusion, our findings suggest that CUS exposure induces testicular dysfunction, which can be prevented by vitamin D, potentially through the regulation of steroidogenic pathways.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"95 ","pages":"Article 102910"},"PeriodicalIF":2.7,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143829420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pluripotent stem cell-derived gametes: A gap for infertility treatment and reproductive medicine in the future","authors":"Golnaz Shafiei ,&nbsp;Sayyed Alireza Talaei ,&nbsp;Seyed Ehsan Enderami ,&nbsp;Mahmood Khaksary Mahabady ,&nbsp;Javad Amini Mahabadi","doi":"10.1016/j.tice.2025.102904","DOIUrl":"10.1016/j.tice.2025.102904","url":null,"abstract":"<div><div>Infertility affects 10–15 % of reproductive-age couples worldwide, with male infertility linked to sperm dysfunction and female infertility caused by ovulation disorders and reproductive abnormalities. Stem cell research presents a promising avenue for infertility treatment through germ cell differentiation. However, standardizing differentiation protocols and ensuring the functionality of <em>in vitro</em>-derived gametes remain significant challenges before clinical application becomes feasible.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"95 ","pages":"Article 102904"},"PeriodicalIF":2.7,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143800261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression pattern of RNA demethylase ALKBH5 in fetal and adult human testis","authors":"Fang Fang ,&nbsp;Ke Ni","doi":"10.1016/j.tice.2025.102901","DOIUrl":"10.1016/j.tice.2025.102901","url":null,"abstract":"<div><div>N⁶-methyladenosine (m⁶A) is a common post-transcriptional modification of RNAs in eukaryotic cells, which is involved in various biological processes. ALKBH5 is one of the m⁶A demethylases and has been reported to play important roles in mouse testis. But the function of ALKBH5 in human testis remained undiscovered. Here we aimed to analyze the expression and location of ALKBH5 in fetal and adult human testis. We found that fetal human testis is characterized by the formation of testis cords filled with pre-spermatogonia and pre-Sertoli cells, which is significantly distinct from the convoluted seminiferous epithelium in adult testis. ALKBH5 is not only widely expressed in adult human testis, but also expressed in VASA positive pre-spermatogonia, SOX9 positive pre-Sertoli cells, and CYP11A positive pre-Leydig cells in fetal human testis. Moreover, bioinformatics analysis of published RNA-sequencing data (GSE63392) revealed the expression of ALKBH5 in human fetal germ cells is upregulated with the increase of gestational weeks. Thus, our results indicate the potential role of ALKBH5 in fetal human testis development and function.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"95 ","pages":"Article 102901"},"PeriodicalIF":2.7,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Echinatin inhibits the growth and metastasis of human hepatocellular carcinoma cells through p38 and JNK signaling pathways","authors":"Jiayu Wang ,&nbsp;Ziyun Li ,&nbsp;Xueqian Han ,&nbsp;Zhou Xie ,&nbsp;Yajun Hou ,&nbsp;Miao Liu ,&nbsp;Yuhang Cheng ,&nbsp;Qiuping Lu ,&nbsp;Jinyong Luo ,&nbsp;Hongbo Wang","doi":"10.1016/j.tice.2025.102907","DOIUrl":"10.1016/j.tice.2025.102907","url":null,"abstract":"<div><div>Hepatocellular carcinoma (HCC) ranks among the most prevalent cancers, with both a high incidence and a significant mortality rate. Clinical medications are highly toxic to patients and prone to resistance. Natural products are highly valued in the development of antitumour drugs. This study aimed to elucidate the anti-HCC ability and potential mechanism of Echinatin (Ecn), a natural existed flavonoid. Our findings revealed that Ecn suppressed the growth, migration, and invasion of HCC cells and demonstrated a superior inhibitory impact on the development of xenograft tumors. Moreover, Ecn was less toxic to mice and had a good drug safety. Mechanistically, Ecn was found to activate p38 and JNK signaling pathways. Accordingly, the suppressive effect of Ecn on HCC cells was attenuated by the introduction of p38 blocker SB203580 and JNK blocker SP600125. Collectively, our research suggests that Ecn might have anti-HCC properties through the activation of p38 and JNK signaling.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"95 ","pages":"Article 102907"},"PeriodicalIF":2.7,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143800265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulatory effect of Eruca vesicaria seeds essential oil on acetamiprid nephrotoxicity via oxidative stress inhibition and regulation of Cox-2, TNF-α, and PPAR-α pathways","authors":"Fatma Mohamady El-Demerdash ,&nbsp;Laith Taha Mohammed ,&nbsp;Tarek Mostafa Mohamed","doi":"10.1016/j.tice.2025.102905","DOIUrl":"10.1016/j.tice.2025.102905","url":null,"abstract":"<div><div>Acetamiprid (Aceta) is a neonicotinoid insecticide utilized extensively worldwide, and its environmental and human health risks are of concern. <em>Eruca vesicaria</em> is an edible year-round plant that contains a lot of health-promoting phytochemicals and is an excellent source of antioxidants. So, the present investigation was planned to assess the effect of <em>E. vesicaria</em> seed essential oil versus acetamiprid-induced toxicity in rats. Animals were partitioned into 4 groups of seven each: control, <em>E. vesicaria</em> seeds essential oil (ESEO; 0.17 mL/kg), acetamiprid (Aceta; 21.7 mg/kg), and ESEO plus Aceta, respectively. Doses were given orally and daily for 14 days. Results revealed that ESEO has many phytochemical components with high antioxidant activity. Data showed that treatment with Aceta increased lipid peroxidation and decreased the activities of “enzymatic and non-enzymatic antioxidants” in kidney homogenate. Also, disturbance of kidney and liver function biomarkers, lipid profile, and protein content were observed. These are confirmed by the histological, molecular (Cox-2, TNF-α, and PPAR-α), and renal damage biomarkers (KIM-1 and Cystatin C) examination. On the other hand, rats administered ESEO and then treated with Aceta showed significant amelioration in most of the examined indices. To sum up, ESEO has a potent anti-inflammatory, anti-apoptotic, and antioxidant activity that protects against the pronounced harmful effects of Aceta in rat kidneys due to its health-promoting phytochemicals.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"95 ","pages":"Article 102905"},"PeriodicalIF":2.7,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resveratrol-loaded nanofibrous scaffolds combined with menstrual blood stem cells for bone healing applications","authors":"Xiaoyong Yang ,&nbsp;Jian Shi ,&nbsp;Yi Chui ,&nbsp;Ting Wang ,&nbsp;Yongqing Xu","doi":"10.1016/j.tice.2025.102900","DOIUrl":"10.1016/j.tice.2025.102900","url":null,"abstract":"<div><div>In the field of regenerative medicine, bone tissue engineering has emerged as a promising strategy for addressing bone defects and injuries. A key aspect of this field is the development of biomimetic scaffolds that replicate the intricate architecture of native bone tissue, creating an environment conducive to cellular attachment, proliferation, and differentiation. In this study, we developed a novel resveratrol-loaded nanofibrous collagen/polycaprolactone (PCL) scaffold designed to serve as a delivery system for menstrual blood stem cells (MenSCs) to enhance bone healing. This innovative approach integrates the osteogenic, anti-inflammatory, and antioxidant properties of resveratrol with the multipotency and immunomodulatory effects of MenSCs, creating a dual-functional system that enhances bone regeneration, angiogenesis, and immune modulation. The scaffolds were extensively characterized in vitro, evaluating their microarchitecture, biological properties, hemocompatibility, radical scavenging potential, and anti-inflammatory activity. They were then implanted into a rat model with calvarial bone defects to assess their regenerative potential. Our findings indicate that the scaffolds exhibited no cytotoxicity toward MG-63 cells and demonstrated significant anti-inflammatory activity in vitro. In vivo assessments further revealed that scaffolds loaded with resveratrol and MenSCs promoted bone healing by enhancing collagen deposition and new bone formation. Moreover, gene expression analysis showed upregulation of type I collagen, b-FGF, and VEGFa, while TNF-α expression was downregulated, indicating an improved osteogenic and immunomodulatory response. In conclusion, our study highlights the potential of resveratrol-loaded, MenSCs-seeded scaffolds as a cutting-edge, biomimetic strategy for bone regeneration, offering a novel cell- and drug-based platform for advancing bone tissue engineering and regenerative medicine.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"95 ","pages":"Article 102900"},"PeriodicalIF":2.7,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Perinatal Nicotine Exposure on Oxidative Stress and BDNF Levels in the Brain Tissue of Offspring Rats: The Protective Role of Vitamin E","authors":"Beyza Güzide Özerol ,&nbsp;Engin Burak Selçuk ,&nbsp;Elif Gürel ,&nbsp;Muhammed Mehdi Üremiş ,&nbsp;Mehmet Gül ,&nbsp;Semir Gül ,&nbsp;Harika Gözde Gözükara Bağ ,&nbsp;Onural Özhan ,&nbsp;Yusuf Türköz","doi":"10.1016/j.tice.2025.102881","DOIUrl":"10.1016/j.tice.2025.102881","url":null,"abstract":"<div><h3>Objective</h3><div>Nicotine, a well-known neurotoxin, induces oxidative stress in fetal tissues, leading to organ damage and fetal growth retardation. This study aims to evaluate oxidative stress parameters in the brain tissue of rat offspring exposed to perinatal nicotine and assess vitamin E's protective effects.</div></div><div><h3>Methods</h3><div>Twenty-five pregnant rats were administered 10<!--> <!-->mg/L of nicotine and 300<!--> <!-->mg/L of Vitamin E in drinking water starting from the first day of gestation. On gestational day 21, some offspring were euthanized to form the prenatal group. The remaining litters were born naturally, and dams received treatments via drinking water during gestation and lactation (6 weeks). After the lactation period, the pups were weaned and directly treated for an additional 9 weeks, resulting in an overall treatment duration of 15 weeks. Brain tissues were analyzed for MDA, GSH, TOS, TAS, OSI, BDNF, Caspase-3 activity, and histopathological changes.</div></div><div><h3>Results</h3><div>The nicotine-exposed pups exhibited significantly reduced crown-rump length, body mass, and brain mass compared to controls. Nicotine exposure decreased BDNF, GSH, and TAS levels and increased MDA, TOS, and OSI levels. Histopathologically, the nicotine prenatal group showed a significantly higher number of heterochromatic nuclei in brain tissue. Caspase-3 activity did not show a significant increase in nicotine groups compared to the control. Vitamin E supplementation mitigated nicotine-induced brain damage in some measured parameters.</div></div><div><h3>Conclusion</h3><div>Perinatal nicotine exposure induces oxidative damage in the brain tissue of rat offspring, while vitamin E exerts a protective antioxidant effect, preventing nicotine-induced neurotoxicity. Furthermore, the significant reduction in BDNF levels and the increase in heterochromatic nuclei in the nicotine-exposed groups highlight the detrimental impact of nicotine on neurodevelopment, which can be effectively mitigated by vitamin E supplementation.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"95 ","pages":"Article 102881"},"PeriodicalIF":2.7,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143776462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activation of the SIRT1/PGC-1α pathway by HNRNPD promotes vasculogenic mimicry in NSCLC","authors":"Xuefeng Zhang ,&nbsp;Peng Meng ,&nbsp;Peng Wang ,&nbsp;Yaobo Song","doi":"10.1016/j.tice.2025.102903","DOIUrl":"10.1016/j.tice.2025.102903","url":null,"abstract":"<div><div>Lung cancer is the predominant cause of cancer-related fatalities worldwide, with 80 % classified as non-small cell lung malignancies (NSCLC), characterized by a low 5-year overall survival rate and elevated mortality. Current therapies for NSCLC include targeted drugs, immunotherapy, and combination treatments. The low survival rates highlight the urgent need for novel NSCLC treatments. Vasculogenic mimicry is a tumor blood supply system devoid of endothelium, consisting of invasive and spreading. Heterogeneous Nuclear Ribonucleoprotein D (HNRNPD) expression is upregulated in various cancers. We conducted both in vitro and in vivo experiments by knockdown and overexpression of HNRNPD. Cell proliferation, invasion and angiogenesis were simulated in vitro. A mouse model of subcutaneous transplanted tumor was constructed in vivo, and pathological and immunohistochemical tests were performed.This study examined the involvement of HNRNPD in vasculogenic mimicry in NSCLC and its underlying mechanism. Our research demonstrated that HNRNPD was significantly expressed in NSCLC cells. Inhibition of HNRNPD expression impeded the activation of the Sirtuin 1 / PPARG Coactivator 1 Alpha (SIRT1/PGC-1α) pathway, thereby lowering the proliferation, invasion, and vascular mimicry capability of NSCLC cells. In vivo tests additionally validated that the suppression of HNRNPD could impede tumor growth and angiogenesis in NSCLC murine models. This presents a novel potential target for the targeted therapy of NSCLC. Our research demonstrated that HNRNPD facilitates vasculogenic mimicry development and tumor progression in NSCLC via activating the SIRT1/PGC-1α pathway, offering a novel approach for targeted therapy in NSCLC.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"95 ","pages":"Article 102903"},"PeriodicalIF":2.7,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143800262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}