Tissue & cell最新文献

{"title":"A preliminary model of an oral dysplastic lesion on a chip","authors":"Roberto Plebani ,&nbsp;Tania Vanessa Pierfelice ,&nbsp;Emira D’Amico ,&nbsp;Mario Romano ,&nbsp;Simonetta D’Ercole ,&nbsp;Giovanna Iezzi ,&nbsp;Morena Petrini","doi":"10.1016/j.tice.2026.103347","DOIUrl":"10.1016/j.tice.2026.103347","url":null,"abstract":"<div><div><em>In vitro</em> models of oral dysplasia fail to recapitulate physiologically relevant tissue-tissue interfaces and other microenvironmental cues. This study aimed to present a preliminary organ-on-a-chip (OoC) model of a precancerous oral cavity lesion (OD-OoC). The objective was to reproduce in a two-channel microfluidic device an <em>in vitro</em> tridimensional (3D) model characterized by an organized interaction between endothelial cells, fibroblasts, and dysplastic oral keratinocytes on a collagen I-coated membrane. On day 1, human umbilical vein endothelial cells (ECs) were introduced in the bottom channel, and the chip was inverted to allow cell adhesion to the lower side of the membrane. The chip was then inverted back to the original position, and human gingival fibroblasts (hGFs) were introduced into the top channel. On day 2, the laminar flow was activated, while uniform layers of hGFs and ECs were forming in the respective channels. On day 3, dysplastic oral keratinocytes (DOKs) were inoculated in the top channel above the hGFs layer. On day 5, the chip was fixed with 4 % paraformaldehyde and stained with antibodies targeting podoplanin, Trop2, and VE-cadherin for staining of hGFS, ECs, and DOKs, respectively. Confocal microscopy confirmed the presence of all cell types, showing fibroblast migration from the top channel to the bottom channel of the chip, where they localized between the membrane and the ECs. DOKs confined to the top channel showed slight and uneven E-cadherin and EpCAM (Epithelial Cell Adhesion Molecule) positivity, but evident positivity for Trop-2, confirming that their phenotype differed from that of healthy epithelial cells. The presented OD-OoC could enable in vitro monitoring of epithelial cell phenotype changes and cell migration across the membrane, suggesting its potential applicability in future oral cancer research.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"100 ","pages":"Article 103347"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146079788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TICAM1 inhibits angiogenesis and accelerates inflammation in the healing of diabetic wounds via the NF-κB pathway","authors":"Shasha Liu ,&nbsp;Qin Xie ,&nbsp;Li Zeng","doi":"10.1016/j.tice.2026.103364","DOIUrl":"10.1016/j.tice.2026.103364","url":null,"abstract":"<div><div>Diabetes and its associated complications have been linked to high glucose environments. Diabetic wound repair is complex, which significantly affects endothelial cell function, and remains a major therapeutic challenge. Currently, few targeted interventions exist to promote effective healing. Here, the function of TICAM1 in diabetic angiogenesis was investigated, aiming to identify potential therapeutic targets to accelerate wound repair. Human umbilical vein endothelial cells (HUVECs) were grown with high glucose or corresponding control conditions and treated with a lentivirus expressing TICAM1-targeting shRNA, Licochalcone D, or a p65 overexpression plasmid. RNA sequencing, Western blotting, tube formation, and quantitative reverse transcription PCR were employed to assess angiogenic capacity and associated signaling pathways. Cell proliferation was evaluated using EdU and CCK-8 assays, and flow cytometry was utilized to measure apoptosis. Cell permeability was assessed using fluorescein isothiocyanate (FITC)-dextran and transendothelial electrical resistance (TEER) assays. Inflammatory mediator levels, including IL-1β, IL-6, and TNF-α, were measured with ELISAs. TICAM1 expression was upregulated in endothelial cells exposed to HG, resulting in reduced angiogenic activity and increased inflammatory cytokine release. Similar endothelial alterations were observed under hyperglycemic conditions across different experimental settings. Knockdown of TICAM1 or treatment with Licochalcone D restored angiogenesis and inhibited inflammation. On the other hand, p65 overexpression reversed the effects of TICAM1 knockdown under HG conditions. In conclusion, TICAM1 impairs angiogenesis and promotes inflammation under HG conditions through the p65-mediated NF-κB axis. These results suggest the potential of TICAM1 in promoting the repair of diabetic wounds.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"100 ","pages":"Article 103364"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146114062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dictyota ciliolata ameliorates hyperglycemia, oxidative stress, and inflammation via modulation of metabolic and signaling pathways in diabetic rats","authors":"Eman Fayad ,&nbsp;Maha A. Aljumaa ,&nbsp;Hanan A. Rizk ,&nbsp;Aml Ghanem ,&nbsp;Amira Hathout ,&nbsp;Amina Rashad Ali ,&nbsp;Dalal Sulaiman Alshaya ,&nbsp;Haitham Ibrahim El-Mekkawy ,&nbsp;Aya Salem ,&nbsp;Osama A. Mohammed ,&nbsp;Karimeldin M.A. Salih ,&nbsp;Ahmed S. Doghish ,&nbsp;Dalia Elebeedy","doi":"10.1016/j.tice.2026.103368","DOIUrl":"10.1016/j.tice.2026.103368","url":null,"abstract":"<div><div>Diabetes mellitus, marked by chronic hyperglycemia and systemic inflammation, causes oxidative stress and multi-organ damage. Marine macroalgae like <em>Dictyota ciliolata</em> contain polyphenols and flavonoids with potential therapeutic effects on metabolic disorders. This study examined the antidiabetic, hepatoprotective, antioxidant, and anti-inflammatory effects of <em>Dictyota ciliolata</em> extract in alloxan-induced diabetic rats. Adult male Wistar rats were allocated into four groups: normal control, diabetic control, diabetic treated with metformin (5 mg/kg), and diabetic treated with <em>Dictyota ciliolata</em> extract (200 mg/kg) for 21 days. Assessments included fasting blood glucose, serum insulin, liver enzymes (ALT, AST, ALP), lipid profile, oxidative stress biomarkers (MDA, SOD, CAT, GPx), pro-inflammatory cytokines (IL-6, TNF-α), and transcription factors (NF-κB). Gene expression of metabolic regulators (PPAR-γ, GLUT4, IRS-1, Nrf2, AMPK) was analyzed via qRT-PCR. Histopathological examination of the liver and pancreas was conducted to assess tissue-level effects. Treatment with <em>Dictyota ciliolata</em> reduced fasting blood glucose by approximately 38 % and increased serum insulin levels by 55 % compared with diabetic controls. The extract restored liver enzyme profiles and lipid parameters while increasing antioxidant enzyme activities by more than 40 % and significantly reducing lipid peroxidation. Furthermore, the extract modulated the levels of inflammatory cytokines. Gene expression analysis confirmed upregulation of PPAR-γ (0.70-fold), GLUT4 (1.42-fold), AMPK (1.17-fold), IRS-1 (1.36-fold), and Nrf2 (1.49-fold) relative to diabetic controls. Histopathological findings supported the biochemical results, revealing preserved hepatic architecture with limited pancreatic recovery. <em>Dictyota ciliolata</em> ethanolic extract exerts multifaceted protective effects in diabetic conditions via regulation of glycemia, oxidative stress, and inflammatory signaling. These findings highlight its potential as a natural therapeutic candidate for diabetes management.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"100 ","pages":"Article 103368"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146133067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paeonol inhibits the progression of endometrial cancer by affecting TRIM26-mediated LDHA ubiquitination modification","authors":"Xu Chen ,&nbsp;Jianxing Liu ,&nbsp;Yongrui Liang ,&nbsp;Zihan Gong","doi":"10.1016/j.tice.2026.103359","DOIUrl":"10.1016/j.tice.2026.103359","url":null,"abstract":"<div><div>Endometrial cancer (EC) is a common gynecological malignancy characterized by abnormal glucose metabolism. Paeonol (Pae), a natural phenolic compound derived from traditional Chinese medicine, exhibits broad-spectrum antitumor activity. However, its role in modulating glycolysis and the underlying molecular mechanisms in EC remain poorly understood. The effects on EC cell viability (CCK-8), proliferation (EdU), apoptosis (flow cytometry), invasion (transwell), migration (wound healing), and tube formation rate were assessed. Glycolytic parameters were measured using corresponding commercial kits. Protein and mRNA expression levels were determined by Western blotting and RT-qPCR. The interaction between tripartite motif protein 26 <strong>(</strong>TRIM26) and lactate dehydrogenase A (LDHA) was investigated through co-immunoprecipitation (Co-IP), cycloheximide (CHX) chase, and ubiquitination assays. A xenograft model was established to examine the <em>in vivo</em> efficacy of Pae. Pae inhibited proliferation, metastasis, tube formation, and glycolysis of EC cells, and induced apoptosis. Pae suppressed EC malignant behaviors by downregulating LDHA expression. TRIM26 promoted ubiquitination-mediated degradation of LDHA. Overexpression of LDHA reversed the tumor-suppressive effects of TRIM26 overexpression in EC cells. TRIM26 knockdown attenuated the antitumor effects of Pae. <em>In vivo</em> experiments demonstrated that Pae inhibited tumor growth and regulated TRIM26/LDHA expression. Pae was found to promote TRIM26 expression, which in turn enhanced TRIM26-mediated ubiquitination and degradation of LDHA, thereby contributing to glycolysis inhibition and suppression of EC progression. These results suggested that Pae might exert its effects by modulating the TRIM26/LDHA axis and supported its potential therapeutic value in EC.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"100 ","pages":"Article 103359"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146189725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic mechanism of Changyong Decoction in suppressing colorectal cancer progression and inducing ferroptosis via the PIEZO1-PI3K/AKT signaling axis","authors":"Mengxue Han ,&nbsp;Shasha Shi ,&nbsp;Zitong Guan ,&nbsp;Wenke Ma ,&nbsp;Xiaolin Wang ,&nbsp;Hao Jiang ,&nbsp;Shuai Man","doi":"10.1016/j.tice.2026.103379","DOIUrl":"10.1016/j.tice.2026.103379","url":null,"abstract":"<div><div>Colorectal cancer is a common type of cancer affecting the digestive system. Its development largely results from unbalanced growth and spread of cancerous cells. Changyong Decoction (CYD), a classical traditional Chinese medicine formula, demonstrates therapeutic effects including blood circulation activation, blood stasis elimination, pus elimination, and mass resolution. Nevertheless, its anti-CRC mechanisms remain incompletely elucidated. This study adopted a comprehensive strategy that integrated network pharmacology, UHPLC-MS/MS, molecular docking, and molecular dynamics simulations to systematically predict the active components, targets, and mechanisms of CYD in the treatment of colorectal cancer (CRC). The antitumor efficacy of CYD was further validated through experimental studies. Network pharmacology identified 10 key targets, with KEGG analysis showing significant enrichment of the PI3K/AKT pathway. UHPLC-MS/MS validation confirmed eight primary bioactive compounds. Molecular docking and molecular dynamics simulations confirmed the stable binding between the main bioactive components of CYD and the key target proteins. The anti-colorectal cancer efficacy of CYD was further assessed in vivo using a zebrafish xenograft model. In vitro studies revealed that CYD established a Piezo1-PI3K/AKT regulatory axis that exerts antitumor effects via multiple mechanisms. In this study, we demonstrated that CYD suppressed tumor progression in colorectal cancer cells by inhibiting proliferation and migration, and inducing ferroptosis and apoptosis. These effects were mediated via down-regulation of Piezo1 expression and synergistic inhibition of the PI3K/AKT signaling pathway.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"100 ","pages":"Article 103379"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146189227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemicals in ethanolic extract of Cinnamomum tamala induce cell cycle arrest, DNA damage and apoptosis in human breast cancer cell lines MDA-MB-231 and MCF-7","authors":"Ishrat Husain ,&nbsp;Anu Chandra ,&nbsp;Mrinal Ranjan Srivastava ,&nbsp;Farzana Mahdi ,&nbsp;Rumana Ahmad","doi":"10.1016/j.tice.2025.103306","DOIUrl":"10.1016/j.tice.2025.103306","url":null,"abstract":"<div><div><em>Cinnamomum tamala</em> (CT), commonly known as ‘Indian bay leaf’ in English and ‘Tejpatta’ in Hindi is a tree belonging to family Lauraceae and is widely used as a culinary condiment with well-known medicinal properties. In the present study, ethanolic extract of dried CT leaves (EECTL) was characterized by HPLC and the antioxidant activity was evaluated using DPPH radical scavenging assay. Anticancer activity of EECTL was evaluated against human breast cancer cell lines MDA-MB-231 and MCF-7 <em>vis-à-vis</em> normal HEK-293 and Vero cells using MTT assay, along with morphological analysis using phase-contrast microscopy. EECTL-induced chromatin condensation and apoptosis were studied using Hoechst and AO/EtBr staining by fluorescence microscopy, as well as by qRT-PCR analysis. Annexin V-FITC/PI double staining for apoptosis detection and cell cycle analysis using PI were performed using flow cytometry. Catechin (R_t = 6.085 min), eugenol (R_t = 7.211 min) and apigenin (R_t = 19.496 min) were identified as the major bioactive components. EECTL was found to induce apoptosis in MDA-MB-231 and MCF-7 cell lines in a dose-dependent manner with IC₅₀ values of 47 and 110 µg/mL, respectively. Interestingly, EECTL did not exhibit any significant effect on normal HEK-293 and Vero cells. The selectivity indices of EECTL with respect to Vero/MDA-MB-231 and HEK-293/MDA-MB-231 were determined as 5.69 and 8.16, respectively, while those for Vero/MCF-7 and HEK-293/MCF-7 were 2.43 and 3.48, respectively. Mechanistically, EECTL caused cell cycle arrest at G₀/G₁ in MDA-MB-231 and sub-G₀ phase in MCF-7 cells, leading to chromatin condensation and apoptosis. DNA damage and fragmentation were confirmed <em>via</em> comet assay. Expression analysis revealed that EECTL upregulated the expression of <em>TP53</em> in MDA-MB-231 and MCF-7 cells. EECTL was found to have significant antioxidant potential against DPPH radicals, with an IC₅₀ of 150 µg/mL. This study is the first to report anticancer potential and mechanism of action of EECTL against breast cancer MDA-MB-231 cells. EECTL shows potential to serve as an adjunct to the main line of treatment in breast cancer and may be of interest for future preclinical and clinical studies aimed at developing integrative breast cancer treatments.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"100 ","pages":"Article 103306"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145981897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distinct impact of splenic denervation and the role of TRPV1 in LIPUS-induced anti-inflammatory milieu in the kidney: Involvement of neurochemicals, Nrf2/HO-1 modulation and change of macrophage phenotype","authors":"Omaima Mohammed Abdelwahed ,&nbsp;Basma Emad Aboulhoda ,&nbsp;Sarah Ali Abdelhameed Gouda ,&nbsp;Tarek Shoukry ,&nbsp;Laila Rashed ,&nbsp;Marwa Alkaffas ,&nbsp;Ebtehal Gamal Abdelhady ,&nbsp;Mansour A. Alghamdi ,&nbsp;Hend Abdalla ,&nbsp;Nivin Sharawy","doi":"10.1016/j.tice.2026.103376","DOIUrl":"10.1016/j.tice.2026.103376","url":null,"abstract":"<div><h3>Background</h3><div>The spleen plays a crucial role during inflammation through its resident macrophages, which may be influenced by modulations in neurochemical levels inside the spleen. Transient receptor potential vanilloid 1 (TRPV1) was reported to modulate the immune responses; however, its role in the spleen remains to be elucidated. We aimed to explore whether TRPV1 influences splenic-derived macrophage phenotypes through alterations in neurochemical levels (Calcitonin Gene-Related Peptide (CGRP), acetylcholine &amp; noradrenaline), and modulation of the Nrf2/HO-1 signaling pathway.</div></div><div><h3>Method</h3><div>Using a glycerol- induced acute kidney injury (AKI) model, we investigated modulation of TRPV1 after splenic denervation (SDN) &amp; capsazepine (CPZ). Furthermore, we assessed the impact of low-intensity pulsed ultrasound (LIPUS), with or without CPZ and/or SDN. The alterations in TRPV1, CGRP, acetylcholine, noradrenaline, macrophage phenotypes, Nrf2, and HO-1 were then evaluated.</div></div><div><h3>Results</h3><div>Our findings revealed that inhibiting TRPV1 or splenic denervation could shift macrophages toward a pro-inflammatory M1 phenotype, associated with decrease in the acetylcholine level and suppression of the Nrf2/HO-1 pathway. Notably, these effects were reversed by LIPUS treatment. TRPV1 influenced the concentrations of CGRP, acetylcholine, and noradrenaline, with significant correlations found between splenic mediators (CGRP and acetylcholine) and the pro-inflammatory M1 phenotype related biomarkers and the Nrf2/HO-1 pathway.</div></div><div><h3>Conclusion</h3><div>Integrity of the splenic innervation showed significant association with CGRP, noradrenaline, and acetylcholine levels. Both splenic nerve and TRPV1 potentially influence the macrophage phenotypes-related biomarkers and the expression of Nrf2/HO-1 pathway.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"100 ","pages":"Article 103376"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146190127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoencapsulated Quercetin Protects Against Testicular Ischemia–Reperfusion Injury Of The Seminiferous Tubules In Rats:Ultrastructural And Biochemical Evidence","authors":"Heba G. Ibrahim ,&nbsp;Adham Ibrahim ,&nbsp;Riham M. El-Moslemany ,&nbsp;Ayat Abdelrahman Abdelrahman Elshazly ,&nbsp;Dina M Rostom","doi":"10.1016/j.tice.2026.103630","DOIUrl":"10.1016/j.tice.2026.103630","url":null,"abstract":"<div><div>Testicular torsion is a urological emergency resulting from twisting of the spermatic cord, leading to acute ischemia. Although surgical detorsion is essential to restore blood flow, it is frequently accompanied by ischemia–reperfusion (IR) injury, which induces oxidative stress, inflammation, apoptosis, and potential infertility. Quercetin (Q) is a naturally occurring flavonoid with potent antioxidant and anti-inflammatory properties; however, its therapeutic application is limited by poor aqueous solubility and low bioavailability.</div></div><div><h3>Aim</h3><div>This study aimed to compare the protective effects of free quercetin and quercetin-loaded lipid nano capsules (QLNC) on seminiferous tubules in a rat model of testicular IR injury.</div></div><div><h3>Methods</h3><div>QLNC prepared using the phase inversion method exhibited a mean particle size of 48.23±2.1<!--> <!-->nm with a high encapsulation efficiency. Adult male rats underwent testicular torsion for 1<!--> <!-->h followed by 2<!--> <!-->h of detorsion. Quercetin or QLNC was administered at an equivalent dose of 20<!--> <!-->mg/kg 30<!--> <!-->minutes prior to detorsion. Blood and testicular tissue were collected for biochemical, histopathological, ultrastructural, and immunohistochemical evaluations.</div></div><div><h3>Results</h3><div>Compared to the IR and IR-Q groups, QLNC treatment markedly preserved seminiferous tubule architecture, reduced spermatogenic-cell depletion, and attenuated interstitial edema, vascular congestion, and inflammatory infiltration. This was supported by significant improvements in Johnsen’s and Cosentino scores. Immunohistochemical analysis revealed a pronounced reduction in inducible nitric oxide synthase expression. Additionally, QLNC significantly restored testosterone levels and reduced oxidative stress and inflammatory biomarkers. Overall, QLNC demonstrated superior protective efficacy compared to quercetin, preserving near-normal histological and biochemical parameters.</div></div><div><h3>Conclusion</h3><div>These findings highlight QLNC as a promising nanotherapeutic strategy for mitigating testicular ischemia–reperfusion injury.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"103 ","pages":"Article 103630"},"PeriodicalIF":2.5,"publicationDate":"2026-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148059873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tumor endothelial cells revisited: Their crucial role in tumor progression","authors":"Domenico Ribatti","doi":"10.1016/j.tice.2026.103317","DOIUrl":"10.1016/j.tice.2026.103317","url":null,"abstract":"<div><div>The vasculature surrounding tumors is not only a transport channel for nutrients, oxygen, and metabolites, but also a pathway for metastasis. This article summarizes the tissue and cellular origin of tumor endothelial cells, analyzes their characteristics, and summarizes their role in tumor progression and metastasis. Due to their distinct characteristics, tumor endothelial cells are a target for anti-angiogenic therapies, which aim to stop tumor blood vessel formation. Emerging therapeutic strategies aim at modulating both tumor endothelial cells and immune cells, not only to block angiogenesis but also to enhance the recruitment and activation of effector cells within the tumor.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"99 ","pages":"Article 103317"},"PeriodicalIF":2.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145967307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immediate and long-term effects of insufficient weight-bearing during growth on hip morphology and histopathology in rats","authors":"Marina Kanehara,&nbsp;Norikazu Nishida,&nbsp;Akinori Kaneguchi,&nbsp;Junya Ozawa","doi":"10.1016/j.tice.2025.103289","DOIUrl":"10.1016/j.tice.2025.103289","url":null,"abstract":"<div><div>Reduced weight bearing during growth can induce bony morphological characteristics of developmental dysplasia of the hip, a risk factor for hip osteoarthritis (OA). However, the long-term effects of reloading remain unknown. This study aimed to evaluate the immediate and long-term effects of hindlimb suspension (HS) during growth on hip bone morphology, alignment, and histopathology. Four-week-old female rats were subjected to HS for four or eight weeks to reduce weight bearing. After 4 or 8 weeks of HS, the rats were reloaded until 54 weeks of age, which corresponds to human middle age. Age-matched animals served as controls. Bone morphology and alignment parameters were measured using three-dimensional reconstructed images from X-ray computed tomography. A histopathological analysis of the femoral head was also performed. Immediately after four and eight weeks of HS, increased femoral anteversion (FeAV) and acetabular anteversion (AcAV), as well as decreased femoral head diameter (characteristics of hip OA), were detected. After reloading until 54 weeks of age, these features persisted, and an increase of distraction index, which are indicators of structural instability, appeared in rats after 4 and/or 8weeks of HS. Furthermore, OA-like changes, such as articular cartilage thinning and subchondral bone sclerosis, were observed in the femoral head after long-term reloading. Additionally, FeAV was significantly correlated with several histopathological indices of femoral head cartilage. These results suggest that reduced weight bearing during growth may lead to hip morphological and alignment abnormalities, which could result in limited OA-like hip joint changes in the long term.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"99 ","pages":"Article 103289"},"PeriodicalIF":2.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145846649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}